• Journal of Korean Society of Environmental Engineers
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• v.27 no.12
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• pp.1277-1284
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• 2005

Alkylphenol Polyethoxylate(APEs) and their metabolites were determined in the aquatic environment in the central Nakdong river basin. The concentrations of APE's ranged between $0.62{\sim}11.70\;{\mu}g/L$ from the Nakdong and the Kumho rivers, and were $70.00{\sim}212.50\;{\mu}g/L$ in the samples from the 3rd industrial complex stream and the Dalseo stream, which are both heavily polluted by industrial wastewater and domestic wastewater. The APEs revealed a removal rate of more than 87% by biodegradation and adsorption etc. in the wastewater treatment plant. Nonylphenol polyethoxylates(NPnEO) and Nonylphenol carboxylic acid(NPnEC) consisted of APE metabolites shifted from NP($n=4{\sim}10$)EO and NP($n=4{\sim}10$)EC to NP($n=1{\sim}3$)EO and NP($n=1{\sim}3$)EC or removed by the adsorption of activated sludge during the biological wastewater treatment process. Upper streams have a higher distributed rate of NP($n=7{\sim}10$)EO than water downstream. Continuous monitoring is necessary for non-point sources as well as point sources, such as a wastewater treatment plant. Effluent concentrations of nonylphenol(NP) in industrial wastewater and domestic wastewater averaged about 4.33 and $1.70\;{\mu}g/L$, respectively. In addition, the removal rate average was 90% in the wastewater treatment plant. NP concentrations in the rivers did not exceed $1.0\;{\mu}g/L$, which are prescribed by environmental risk concentration in the USA and Europe. However, NP required continuous monitoring, which detected over $0.1\;{\mu}g/L$ in all river areas.

• Analytical Science and Technology
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• v.29 no.3
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• pp.114-125
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• 2016

In this study, 165 wastewater discharge facilities in 10 business types were investigated with regard to 24 specific hazardous substances that included heavy metals, VOCs, CN, and phenol in the Gwangju city. Cu in the range from from 0.008 to 35.420 mg/L was detected in all business types and the detection rate was 46.8 %. Other heavy metals, such as Cd, As, Hg, Pb, and Cr⁺⁶ were detected as well. However, their detection rates ranged between 0.6 and 1.8 %. CN and phenol were detected in one and five facilities, respectively. 12 species of VOCs were detected: chloroform 80.6 % (0.42 to 81.60 μg/L), benzene 16.4 % (1.49 to 3.31 μg/L), trichloroethylene 11.5 % (1.78 to 6.02 μg/L), 1,1-dichloroethylene 10.3 % (1.23 to 5.89 μg/L), and dichloromethane 8.5 % (0.28 to 968.86 μg/L) in the detection rate order. The concentration of VOCs was detected in trace amounts, except for dichloromethane that exceeded the effluent quality standard in three business types, namely, metal manufacturing, food industry, and car washing facility. Chloroform was detected in all business types, where 24.88 μg/L were detected in the laundry business and 53.41 μg/L in the water supply business; the mean concentration of chloroform in these two business types was higher than elsewhere. Therefore, for the disposal of non-degradable specific hazardous substances in industrial wastewater, it is necessary to introduce physical and chemical processes, such as activated carbon adsorption, fenton oxidation, ozone treatment, as well as photocatalyst and the UV radiation.

• Korean Chemical Engineering Research
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• v.49 no.2
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• pp.187-194
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• 2011

In compliance with an international law about the ocean dumping of the sludge, the proper sewage treatment process which occurs from the wastewater treatment process has been becoming problem. Generally the sewage and the sludge are controlled from anaerobic condition when the sewage is treated and land filled, where the methane$(CH_{4})$ and the nitrous oxide $(N_{2}O)$ from this process are discharged. Because these gases have been known as one of the responsible gases for global warming, the wastewater treatment process is become known as emission sources of green house gases(GHG). This study is to suggest a new approach of estimate and environmental assessment of greenhouse gas emissions and sludge emissions from wastewater treatment processes. It was carried out by calculating the total amounts of GHG emitted from biological wastewater treatment process and the amount of the sludgegenerated from the processes. Four major biological wastewater treatment processes which are Anaerobic/Anoxic/Oxidation$(A_{2}O)$, Bardenpho, Virginia Initiative Plant(VIP), University of Cape Town(UCT)are used and GPS-X software is used to model four processes. Based on the modeling result of four processes, the amounts of GHG emissions and the sludge produced from each process are calculated by Intergovernmental Panel on Climate Change(IPCC) 2006 guideline report. GHG emissions for water as well as sludge treatment processes are calculated for environmental assessment has been done on the scenario of various sludge treatments, such as composting, incineration and reclamation and each scenario is compared by using a unified index of the economic and environmental assessment. It was found that Bardenpho process among these processes shows a best process that can emit minimum amount of GHG with lowest impact on environment and composting emits the minimum amount of GHG for sludge treatment.

• Korean Journal of Ecology and Environment
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• v.36 no.3 s.104
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• pp.322-335
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• 2003

Nutrient fluxes and sediment oxygen demands (SOD) were measured with intact sediment cores collected from three stations in the downstream of Nagdong River. The sediments were subjected to controlled oxic and hypoxic conditions and temperature gradients (from $10^{\circ}C$ to $30^{\circ}C$) of the overlying waters in laboratory batch system. The effect of temperature and labile layer thickness of the sediment on SOD were examined. $PO_4\;^{3-}$ and $NH_4\;^+$ fluxes were elevated above $20^{\circ}C$ and large mobilities were observed when they were coupled with a hypoxic and high-temperature condition. In the well oxygenated conditions, $PO_4\;^{3-}$ fluxes were negative or negligible but $NH_4\;^+$ fluxes ranged from 1.3 mg N $m^{-2}\;hr^{-1}$ to 2.3 $m^{-2}\;hr^{-1}$. Temperature quotients($Q_{10}$) of $PO_4\;^{3-}$ fluxes were 3.7 ${\sim}$ 7.3 ranges to have the most high values. $PO_4\;^{3-}$ and $NH_4\;^+$ fluxes had the logarithmic increase with temperature, while $NO_3\;^-$ was negatively absorbed to the sediment and linearly correlated with the temperature. $SiO_2$ fluxes showed no difference among oxic and hypoxic conditions and sediment texture. The nutrient fluxes would be closely correlated with pore water chemistry of sediments and activated by the top sediment layer composition such as labile organic matters or algal detritus. The ecological implications of the nutrient fluxes were discussed in terms of sources and sinks of nutrients coupled to algal productions in the Nagdong River.

• Journal of Animal Environmental Science
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• v.8 no.1
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• pp.9-16
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• 2002

This study was conducted to develope the new solid separating system which can be efficiently and economically removed the solid parts in high pollutants concentration of pig slurry. The pollutants concentration, BOD$_{5}$ , COD and SS of the slurry used in this study was 15,990($\pm$2,389)mg/l, 20,004($\pm$5,512)mg/l and 26,486($\pm$5,935)mg/l, respectively. After removal of solid part in slurry, the pollutants concentration, BOD$_{5}$, COD and SS was change into 5,617($\pm$690)mg/l, 5,553($\pm$633)mg/land 1,456($\pm$341)mg/l, respectively in the Fixed biological membrane tank. The reduction of the pollutants concentration of suspend liquid through membrane will be allowed to greatly improve the water purification by an Activated sludge method. This separating system consisted of a temporary storage, a circulating tank and a Fixed Biological membrane tank. A temporary storage which has a draining system of screw type and an aeration device played a tremendous role in draining the solid by filled an aeration of 0.3 l/min. A Fixed Biological membrane tank of which a styrofoam filled in a 2/3 volume as a Biological media was fixed by a stainless steel net (pore size : 0.5mm) to separate the liquid layer of influx in them. The separating system efficiency factors were the speed of screw motor, cycle number of slurries in a circulating tank and moisture contents of solid effluent through the screw path. Although the pollutants concentration was very variable in temporary storage, the final concentration of $BOD_5$ and SS, except COD of the suspended liquid in a Fixed biological membrane were not different regardless of cycle number of a circulating tank. Moisture contents of effluent from temporary storage was 73% under the speed 1 ppm of screw motor and 62% under the 1/4rpm of it.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.3 no.2
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• pp.1-15
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• 1983

This paper has concentrated on estimating the possibility and mathematical analysis for the application of BFB to the treatment of nightsoil with low dilution rate. The experiment for the study of this purpose was conducted by continuous type reactor at $20^{\circ}C$, varying F/M ratio from 0.12 to 0.37 and dilution ratio from 2 to 10, and in it provided matted reticulated polypropylene sheets for the solid supports. The obtained results showed that the application of BFB to the treatment of nightsoil would be more effective than any other biological treatment process. Also, it has observed that the optimum dilution ratio was about 5 times and the optimum HRT was about 17 hours, and then it was estimated that the reactor volume and the quantity of weak water could be reduced to the extent of 70 percent and 80 percent. The experimental results of BFB could be analysed by the mathematical models applied to complete mixing activated sludge process. The substrate removal rates which were obtained by McKinney's($K_m$) and EcKenfelder's($K_e$) equation was 1.784/hr and $2.0{\times}10l/mg{\cdot}day$, and substrate was removed very rapidly compared to those of conventional type biological treatment processes. The biomass yield coefficient($a_5$), the endogeneous respiration rate(b), the synthesis oxygen demand rate($a{_5}^{\prime}$), and the endogeneous respiration oxygen demand rate(b') were 0.349, 0.0237/day, 0.495 and 0.0336, respectively.

• Journal of the Mineralogical Society of Korea
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• v.19 no.4 s.50
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• pp.277-289
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• 2006

This study was carried out to find a new material having high removal efficiency for the harmful red tide. C. polykrikoides grow very fast and accumulate into dense and visible patches near the surface of the seawater ('Water bloom'). Some mineral medicines and Hwangto (reddish soil consist of clay minerals and Fe-oxides) were used in this study to remove C. polykrikoides. The pre-determined sprinkling ratio of mineral vs. seawater which contains approximately 5,000 cells/mL of C. polykrikoides was 10 g/L. In order to quantify the removal efficiency, the density of living cells was measured by counting with the Intervals of 0, 10, 30, and 60 minutes after sprinkling. Five Hwangtos feom different localities were examined in this study. It is found that a material with a high concentration of Fe and Al was the most effective to remove C. polykrikoides. After the sprinkling of the Hwangto showing the best removal efficiency in the test, 99% of total algaes were found to be eliminated within 60 minutes. Jeokeokji showed the highest removal efficiency among clay mineral medicines(92% removal efficiency after 60 minutes), and the rests in decreasing order are as follows: Gamto (91%) > Baekseokji (89%) > Hydromica (81%). In addition, Fe-oxide mineral medicine similarly looking as fine-grained earthy Daejaseok showed 100% removal efficiencyafter 30 minutes, and Wooyoeryang, 95% after 60 minutes. It is noted that even little addition (1 g/L) of Daejaseok, 10% of Hwangto concentration into seawater showed the removal efficiency of 100% after 60 minutes. From the results, it could be concluded that the fine-grained earthy Daejaseok was the most effective natural mineral medicine to remove the C. polykrikoides from seawater. Under the microscope the removal mechanism was found to be activated in the following order: adsorption, swelling of chain colony, chain colony crisis and algaecide.

• Applied Microscopy
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• v.24 no.2
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• pp.78-92
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• 1994

Lysozyme has been reported to be present in the secretory granules of the Paneth cell, and lysozyme immunoreactivity has been detected by immunogold method in Paneth cells of the intestine of human, mouse and rat. The present study was aimed at clarifying the intracellular distribution and changes of the lysozyme immunoreactivity in rabbit Paneth cell after common bile duct ligation of rabbit, using the electron microscope immunogold technique. Healthy adult rabbits weighing about 2kg body weight were divided into normal and bile duct ligated groups. Common bile duct ligation was performed aseptically under ether anesthesia. Experimental animals were sacrificed on the 1st, the 3rd, the 5th, the 7th and the 14th day after the operation. Mucosal specimens from the intestinal gland of ileum were fixed in 2.5% glutaraldehyde-1.5% paraformaldehyde, followed by 1% osmium tetroxide, embedded in araldite mixture, cut with LKB-V ultratome. Ultrathin sections were placed on parlodion coated nickel grids (200mesh). The section-bearing grids were floated upside down on the added substance in a moist chamber at room temperature except for the primary antibody step, which was at $4^{\circ}C$. Sections were etched with a saturated solution of sodium m-periodate for 60min. After etching, sections were pretreated with 0.02M tris buffered saline (TBS), pH 8.4, with 1% bovine serum albumin (BSA, Sigma) for 60min, then treated polyclonal rabbit anti-human lysozyme (Dakipatts) diluted 1 : 50 in TBS with 0.1% BSA for 20hr. Subsequently, grids were incubated 60min in biotinylated goat anti rabbit IgG (Amersham) diluted 1 : 100 in TBS with 0.1% BSA. After this, sections were incubated 60min on streptavidin gold G10 (Amersham) diluted 1 : 50 in TBS with 0.1% BSA. After each step, the grids were briefly rinsed with TBS with 0.1% BSA. After the strepavidin gold step, the sections were jet washed with distilled water. Counterstain of the sections performed by uranyl acetate and lead citrate, and observed with JEM 100 CX II electron microscope. Observed results were as follow; 1. Secretory granules of mouse Paneth cells have a lysozyme immunoreactivity and also eosinophil leucocyte of rabbit applied for the positive-control stain, are well labeld with gold particles. 2. Normal rabbit Paneth cells have a lysozyme immunoreactivity restricted on the secretory granules. 3. Amount lysosomes containing myelin figures in the Paneth cells were significantly increased from 5th day after the common bile duct ligation. 4. Immunoreactivity of Paneth cell secretory granules were more activated on the 3rd day after the common bile duct ligation as compared with those of the normal animal. But the lysozyme immunoreactivity were decreased from the 5th day after the common bile duct ligation. 5. Considering the above finding, lysozyme contained Paneth cell are affected following of common bile duct ligation, whereas lysosomes containing myelin-figure do not exhibit any immunoreactive relationship with those of secretory granules.

• Journal of the Korean Society for Marine Environment & Energy
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• v.9 no.1
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• pp.14-20
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• 2006

In this study, the feasibility of simultaneous removal of organic materials and nitrogen in the waste-water from fisheries processing plant was evaluated using entrapped mixed microbial cell technique(EMMC) process. The experiment was performed using activated sludge from municipal sewage treatment plant which was immobilized with gel matrix by cellulose triacetate. It was found that the stable operation at the treatment system which is composed of anoxic and oxic tank, was possible when the organic and nitrogen loading rates were increased stepwise. The organic and nitrogen loading rates were applied from 0.65 to $1.72kgCOD/m^3/d$ and from 0.119 to $0.317kgT-N/m^3$ with four steps, respectively. The maximum nitrogen loading rate which could satisfy the regulated effluent standard of nitrogen concentration, was $0.3kgT-N/m^3/d$. The removal efficiency of total nitrogen was decreased apparently as increasing nitrogen loading rates, whereas the removal efficiency of ammonium nitrogen was effective at the all tested nitrogen loading rates. Therefore, it was concluded that nitrification was efficient at the system. Nitrate removal efficiency ranged from 98.62% to 99.51%, whereas the nitrification efficiency at the oxic tank ranged 94.0% to 96.9% at the tested loading rates. The removal efficiencies of chemical oxygen demand(COD) and those of total nitrogen at the entire system ranged from 94.2% to 96.6% and 73.4% to 83.4%, respectively.

• Korean Journal of Plant Resources
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• v.29 no.1
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• pp.1-10
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• 2016

Obesity is a pro-inflammatory state that contributes to the development of metabolic disorders such as hyperlipidemia, insulin resistance, type 2 diabetes, non-alcoholic fatty liver, and cardiovascular disease. In this study, we evaluated the inhibition of adipogenesis in 3T3-L1 cells and in high-fat diet (HFD)-induced obese mice by Peucedanum japonicum Thunberg L. water extract (PJT). Lipid accumulation measurement indicates that PJT markedly inhibited adipogenesis in a dose-dependent manner. RT-PCR results demonstrated that the mRNA expression of adipogenic transcription factors such as peroxisome proliferator-activated receptor-γ (PPARγ) and CCAAT/enhancer binding protein-α (C/EBPα) in 3T3-L1 cells were significantly down-regulated by PJT treatment. Oral administration of PJT (100, 300, and 500 ㎎/㎏, b.w/daily for 4 weeks) was conducted in high-fat diet induced obese mice and C57BL/6 mice. The PJT-administered group of HFD-induced mice had a lower body weight gain, along with decreased serum levels of glucose, triglycerides, and total cholesterol compared with the control mice, however, the HDL-cholesterol/total cholesterol ratio was increased. Furthermore, the elevated mRNA expression levels of adipogenesis related genes in the white adipose tissue of obese mice were significantly suppressed by PJT. These results indicate that PJT exhibits anti-obesity effects in obese mice by decreasing in serum lipid levels and lipogenesis related gene.