• 상하수도학회지
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• 제18권4호
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• pp.480-485
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• 2004

Fatty acid methyl ester (FAME) technology was evaluated as a monitoring tool for quantification of Nocardia amarae causing a nuisance foaming problem in activated sludge process. The identified signature peak was 19:1 alcohol as a reliable unique peak to N. amarae. Chemostat study revealed that the distribution and quantity of fatty acid peaks were dependent on the growth stage of Nocardia. The FAME results were similar for two relatively high dilution rates; however, the amounts of signature peaks extracted from the 4 and 6 day cultures were significantly higher. This dependence of signature peaks on the physiological state of the organism may be a useful information to assess the health of microbial populations in activated sludge. A laboratory scale batch foaming potential experiment provided a critical foaming level depending on Nocardia population. This critical Nocardia level determined in this study was in terms of either the threshold filament intersections number or the threshold signature FAME amount. The threshold peak area of signature FAME (19:1 alcohol) and corresponding filament counts were 430PA/mg VSS and $1.45{\times}10^6$ intersections/g VSS, respectively. The threshold signature FAME level could be effectively applied as a criterion for diagnosing foam occurrence in activated sludge system.

• 상하수도학회지
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• 제11권3호
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• pp.67-78
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• 1997

The objective of study was to examine to transient response of hydraulic shock loading in activated sludge process for treatment of municipal sewage. The general experiment approach was to operate the system under steady-state(pre-shock), then to apply step changes during 24hours in fourfold hydraulic shock loading at the same organic loading. Performance was assessed in both the transient state and the new steady-state(post-shock). Three bench scale activated sludge reactors were operated to investigate the effect of fourfold hydraulic shock loading on TSS and COD removal efficiency. In activated sludge reactors operated with 13hours and 7hours of HRT, effluent quality of all reactors was not changed for few effects, and also showed no foaming and no sludge bulking. Those results are the same as sludge withdrawn reactors. The effect of fourfold hydraulic shock loading on the activated sludge reactors operated with 3hours of HRT was most severe. The effluent quality was deteriorated significantly and generate foaming in reactors. Less than 24hours after the fourfold shock loading applied, the activated sludge system seemed to attain a new steady-state condition as show by effluent.

• 한국환경과학회:학술대회논문집
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• 한국환경과학회 1998년도 가을 학술발표회 프로그램
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• pp.2-4
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• 1998

Proliferation of Nocardia amarae cells in activated sludge has often been associated with the generation of nuisance foams. Despite intense research activities in recent years to examine the causes and control of Nocardia foaming in activated sludge, the foaming continued to persist throughout the activated sludge treatment plants in United States. In addition to causing various operational problems to treatment processes, the presence of Nocardia may have secondary effects on the fate of heavy metals that are not well known. For example, for treatment plants facing more stringent metal removal requirements, potential metal removal by Nocardia cells in foaming activated sludge would be a welcome secondary effect. In contrast, with new viosolid disposal regulations in place (Code o( Federal Regulation No. 503), higher concentration of metals in biosolids from foaming activated sludge could create management problems. The goal of this research was to investigate the metal sorption property of Nocardia amarae cells grown in batch reactors and in chemostat reactors. Specific surface area and metal sorption characteristics of N. amarae cells harvested at various growth stages were compared. Three metals examined in this study were copper, cadmium and nickel. Nocardia amarae strain (SRWTP isolate) used in this study was obtained from the University of California at Berkeley. The pure culture was grown in 4L batch reactor containing mineral salt medium with sodium acetate as the sole carbon source. In order to quantify the sorption of heavy metal ions to N amarae cell surfaces, cells from the batch reactor were harvested, washed, and suspended in 30mL centrifuge tubes. Metal sorption studies were conducted at pH 7.0 and ionlc strength of 10-2M. The sorption Isotherm showed that the cells harvested from the stationary and endogenous growth phase exhibited significantly higher metal sorption capacity than the cells from the exponential phase. The sequence of preferential uptake of metals by N. amarae cells was Cu>Cd>Ni. The specific surFace area of Nocardia cells was determined by a dye adsorption method. N.amarae cells growing at ewponential phase had significantly less specific surface area than that of stationary phase, indicating that the lower metal sorption capacity of Nocardia cells growing at exponential phase may be due to the lower specific surface area. The growth conditions of Nocardia cells in continuous culture affect their cell surface properties, thereby governing the adsorption capacity of heavy metal. The comparison of dye sorption isotherms for Nocardia cells growing at various growth rates revealed that the cell surface area increased with increasing sludge age, indicating that the cell surface area is highly dependent on the steady-state growth rate. The highest specific surface area of 199m21g was obtained from N.amarae cell harvested at 0.33 day-1 of growth rate. This result suggests that growth condition not only alters the structure of Nocardia cell wall but also affects the surface area, thus yielding more binding sites of metal removal. After reaching the steady-state condition at dilution rate, metal adsorption isotherms were used to determine the equilibrium distributions of metals between aqueous and Nocardia cell surfaces. The metal sorption capacity of Nocardia biomass harvested from 0.33 day-1 of growth rate was significantly higher than that of cells harvested from 0.5- and 1-day-1 operation, indicatng that N.amarae cells with a lower growth rate have higher sorpion capacity. This result was in close agreement with the trend observed from the batch study. To evaluate the effect of Nocardia cells on the metal binding capacity of activated sludge, specific surface area and metal sorption capacity of the mixture of Nocardia pure cultures and activated sludge biomass were determined by a series of batch experiments. The higher levels of Nocardia cells in the Nocardia-activated sludge samples resulted in the higher specific surface area, explaining the higher metal sorption sites by the mixed luquor samples containing greater amounts on Nocardia cells. The effect of Nocardia cells on the metal sorption capacity of activated sludge was evaluated by spiking an activated sludge sample with various amounts of pre culture Nocardia cells. The results of the Langmuir isotherm model fitted to the metal sorption by various mixtures of Nocardia and activated sludge indicated that the mixture containing higher Nocardia levels had higher metal adsorption capacity than the mixture containing lower Nocardia levels. At Nocardia levels above 100mg/g VSS, the metal sorption capacity of activate sludge increased proportionally with the amount of Noeardia cells present in the mixed liquor, indicating that the presence of Nocardia may increase the viosorption capacity of activated sludge.

• 한국환경보건학회지
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• 제20권2호
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• pp.28-38
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• 1994

New problems have been recently posed on the abnormal foaming (Scum) in an aeration tank and the sludge flotation in a final sedimentation tank during the activated sludge process. However, the activities of the causing bacteria, Nocardia-amarae in an aeration tank have not been searched out at all. Therefore, in this article the activities of Nocardia-amarae in an aeration tank have been closely examined by means of the changes of (F/M) ratio, SRT and inflowing substrate using continuous type and fed-batch type. Summarized results of experiments are as follows. 1. Regrading continuous culture when synthetic wastewater was used substrate neither the increase in the number of Nocardia-amarae in the aeration tank nor the Occurrence of Scum was observed. 2. In the case of fed-batch culture, Nocardia-amarae in the aeration tank increased due to the partial change in substrate and the effect of SRT was significant. 3. Once the scum was formed and the quantity of added Nocardia-amarae and substrate were not changed, the effect of STR was not significant.

• Journal of Microbiology
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• 제37권2호
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• pp.66-72
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• 1999

Mycolic acid-containing actinomycetes associated with extensive foaming in the aeration basin of the activated sludge process were isolated and analyzed by phenotypical, chemotaxonomical and phylogenetic methods. Whole cell sugar patterns of two isolates were pattern A. The nearly complete sequences of the 16S rRNA genes (rDNAs) of the isolates were determined and compared by using several tree-making algorithms. With polyphasic methods, strain SCNU1 was identified as Gordona sputi, and strain SCNU5 assigned to the genus Tsukamurella. The presence of opportunistic pathogens of chronic lung infections within foams can cause public health problems and render waste-treatment processes inefficient.

• 대한환경공학회지
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• 제36권3호
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• pp.172-177
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• 2014

가압형 부상법에 비해 낮은 압력에서 미세기포를 발생시키는 저압형 부상법을 슬러지 부상 농축에 적용하여 그 성능을 검증하고 실제 적용가능성을 확인하였다. 파일롯 규모의 저압형 부상조를 충남 N.S. 하수처리장에 설치하여 혼합슬러지의 농도, 응집제 주입량 및 혼합슬러지 대비 미세기포수의 비율과 같은 운전 변수가 혼합슬러지 농축에 미치는 영향을 확인하였다. 미세기포는 내부 압력이$1.5kgf/cm^2$으로 유지된 미세기포발생기에서 공기와 기포조제가 포함된 물을 고속 충돌 방식으로 발생시켰으며, 이를 부상농축 실험에 사용하였다. 장기운전 시 유입된 혼합슬러지의 SS 농도는 평균 14,400 mg/L였으며, 응집제 농도 27.6 mg/L, 기포조제 농도 4.0 mg/L, 혼합슬러지 대비 미세기포수의 비를 9.7%로 하여 저압형 부유부상조를 운전한 결과, 60,300 mg/L의 농축슬러지 고형물함량과 99% 이상의 고형물 회수율을 얻었다. 이 경우의 고형물 표면적 부하율은 $30kg/m^2/hr$로 2011년 환경부에서 제정한 하수도 상압 부상농축 시설기준 25 $kg/m^2/hr$을 상회하였다.

• 한국물환경학회지
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• 제26권1호
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• pp.81-88
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• 2010

This study was conducted to find the capability of comparison of overall oxygen transfer coefficient in the membrane coupled high performance reactor (MPHCR) in treating high organic loading wastewater. Effluent quality had been analyzed while the influent organic loading rate was changed from 2 to $7kg\;COD/m^3{\cdot}day$. The oxygen transfer coefficients had been investigated using two-phase nozzle for operating variables which were internal circulation flowrate (5~8 L/min), air flow rate (0.0125~0.2 L/min), liquid temperature ($10{\sim}20^{\circ}C$), and pure-oxygen flow rate (0.0125~0.2 L/min). The overall oxygen transfer coefficient was increased with flowrate of internal circulation and air and high temperature. Especially, internal circulation flow rate showed distinct effect on overall oxygen transfer coefficient due to an increase of gas holdup and air-liquid contract area by two-phase nozzle. In the high range of organic loading rate from 4 to $7kg\;COD/m^3{\cdot}day$, the removable efficiency of COD was 91%. Conventional activated sludge process usually treat organic loading from 0.32 to $0.64kg\;COD/m^3{\cdot}day$ however, the MPHCR can treat 10 to 20 times higher if it would be compared to the conventional activated sludge process. Foaming problem often happened and caused biomass wash out of the reactor, therefore, the foaming should be controlled for the enhanced operation.