• Title/Summary/Keyword: acidophilic $\alpha$-amylase

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Cloning and Expression of an Acidophilic $\alpha$-Amylase Gene from Bacillus circulans in Escherichia coli (Bacillus circulans의 호산성 $\alpha$-amylase 유전자의 클로닝 및 발현)

  • 이종석;김지연;김한복;이동석
    • Korean Journal of Microbiology
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    • v.36 no.2
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    • pp.112-118
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    • 2000

  • A new gene encoding an acidophilic TEX>$\alpha$-amylase of Bacillus cil-culans KCTC3004 was cloned into Eschericlzia coli using pUC19 as a vector. The gene localized in the 5.8 kb PstI DNA fragment was expressed independently of its orientation in the cloning vector showing enzyme activity about 40 times greater than that produced by the original B, circulans The optimum pH and temperature of the cloned enzyme were pH 3.6 and 45^{\circ}C.$ respectively. The enzyme hydrolyzed starch to produce maltotriose and maltooligosaccharides. The SDS-PAGE and zymopram of the enzyme produced in E coli(p.4L850) indicated a molecular weight of 55,000.

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