• 제목/요약/키워드: acidic treatment

검색결과 574건 처리시간 0.035초

Isolation and Differential Expression of an Acidic PR-1 cDNA Gene from Soybean Hypocotyls Infected with Phtophthora sojae f. sp. glycines

  • Kim, Choong-Seo;Yi, Seung-Youn;Lee, Yeon-Kyung;Hwang, Byung-Kook
    • The Plant Pathology Journal
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    • 제16권1호
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    • pp.9-18
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    • 2000
  • Using differential display techniques, a new acidic pathogenesis-related (PR) protein-1 cDNA (GMPRla) gene was isolated from a cDNA library of soybean (Glycinemax L.Merr, cultivar Jangyup) hypocotyls infected by Phytophthora sojae f. sp. glycines. The 741 bp of fulllength GMPRla clone contains an open reading frame of 525 nucleotides encoding 174 amino acid residues (pI 4.23) with a putative signal peptide of 27 amino acids in the N-terminus. Predicted molecular weight of the protein is 18,767 Da. The deduced amino acid sequence of GMPRla has a high level of identity with PR-1 proteins from Brassica napus, Nicotiana tabacum, and Sambucus nigra. The GMPRla mRNA was more strongly expressed in the incompatible than the compatible interaction. The transcript accumulation was induced in the soybbean hypocotyls by treatment with ethephon or DL-$\beta$-amino-n-butyric acid, but not by wounding. In situ hybridization data showed that GMPRIa mRNAs were usually localized in the vascular bundle of hypocotyl tissues, especially phloem tissue. Differences between compatible and incompatible interactions in the timing of GMPRla mRNA accumulation were remarkable, but the spatial distribution of GMPRla mRNA was similar in both interactions. However, more GMPRla mRNA was accumulated in soybean hypocotyls at 6 and 24 h after inoculation.

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외인성 및 내인성 요인에 의한 치아부식에 대한 치료 증례 (Treatment of dental erosion caused by intrinsic and extrinsic etiology: a case report)

  • 이경제;진수윤;김희중;민정범
    • 구강회복응용과학지
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    • 제33권1호
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    • pp.42-46
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    • 2017
  • 부식이란 산성의 화학적 물질에 의한 치아 경조직의 손상으로 정의된다. 이에 대한 원인인 산성 음식, 약물, 작업환경 등의 외부적 원인과 위장관 장애에 의한 위산의 역류나 거식증에 의한 의도적 구토의 내부적 원인에 의해 발생된다. 이는 임상적 증상의 심각도에 따라 레진 충전, 라미네이트 수복 또는 전장관 수복으로 치료한다. 본 증례는 잦은 산성 과일의 섭취로 인한 전치부 순측 부식 및 거식증으로 인한 의도적 구토로 전치부 구개측 부식이 일어난 드문 상황에 대한 치료이다.

크롬유혁의 인공땀액 처리에 의한 물성변화 (Changes on Physical Properties of Chrome Leather by Treatment with Artificial Perspiration)

  • Yoon, Jeong Ah;Choi, Suk Chul;Lee, Yang Hun;Chun, Tae Il
    • 한국염색가공학회지
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    • 제7권4호
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    • pp.25-32
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    • 1995
  • This study was carried out to examine the influences of fatigue and perspiration on the deterioration of chrome leather for shoes. The samples were treated with acidic and alkaline artificial perspirations and applied cyclic tensile stress, and the elastic recovery from fatigue strain, tensile strength and elongation, stiffness etc. by regions and directions were determined. The physical properties generally indicate considerable difference by regions and directions due to the thickness, entangling state, and arrangement direction of fiber buldles within the leather. By the treatment of artificial perspirations, the length, weight, strength, elastic recovery from fatigue of the lether are decreased and the thicknes, elongation, and stiffness are increased; and their extents by the alkaline perspiration are higher than those by the acidic one. The elastic recovery from fatigue of samples are decreased with increasing fatigue cylcle; and indicate regressive relations against the logarithm of the elapsed time, and the means of regression coefficients are 2.73 > 2.55 > 2.22 for samples treated by the acidic perspiration, untreated, and treated by the alkaline perspiration, respectively. The tensile strength is decreased and elogation is increased by fatigue, but the changes are diminished likely due to a rearrangment of fiber bundles with increasing fatigue cycle. And the samples become a little flexible immediately after fatigue; but stiffen again with elastic recovery from fatigue strain, consequently for some cases their stiffness after recovery are rather greater than those before fatigre.

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국내산 규조토의 수열반응에 의한 Kaolinite질 인공점토의 합성에 관한 연구 (The Synthesis of Kaolinitic Clay Minerals from Domestic Diatomite by Hydrothermal Process)

  • 김남일;박계혁;정창주
    • 한국세라믹학회지
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    • 제31권11호
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    • pp.1401-1413
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    • 1994
  • The synthesis of kaolinite mineral from domestic diatomite for silica resource, commercial vailable gibbsite or alumina for alumina resource were made under various hydrothermal treatment, and the sythetic effect of acidic mineralizers, temperature treatment with time duration, particle size of alumina on formation of kaolinite mineral and the plastic properties of synthesized kaolinite were investigated. The various acidic mineralizers which are HCl, HNO3, H2SO4 and Oxalic acid were employed for hydrothermal reaction in the range of 0.01 mol/ι to 2 mol/ι concentration of each mineralizers. It was found that HCl in the level of 1 mol/ι solution produced highly yields of well-crystallized and platy form kaolinite mineral and gave the most effective extraction of iron oxide, compared to that of others, that HNO3 produced highly yield of kaolinite but lower extraction of iron oxide, that H2SO4 produced low yield of kaolinite and formed alunite mineral, and that oxialic acid formed spherical crystalline kaolinite and gave low extraction of iron oxide. Moreover, it showed that kaolinite minerals were well synthesized in a wide range of less than 2 mol/ι acids, but were poorly synthesized at more than 2 mol/ι acids. However, boehmite and kaolinite were coexistently formed in the temperature range of 18$0^{\circ}C$ and 20$0^{\circ}C$ when the calcined diatomite and gibbsite were involved. The well-ordered kaolinite mineral as a platy form was highly synthesized in the temperature range of 220 and 24$0^{\circ}C$, when the same marterials as above were used with treatment of 1 mol/ι HCl solution. The results also revealed that the size of crystalline platy form kaolinite, synthesized from alumina and calcined diatomite with treatment in 1 mol/ιHCl solution at 24$0^{\circ}C$, was much larger than that of gibbsite and calcined diatomite shown previously, and that kaolinite and corundum minerals were coexistently formed under any hydrothermal treatment conditions. The plasticity of synthesized kaolinite from under 2 ${\mu}{\textrm}{m}$ alumina and calcined diatomite was very poor, and that of the synthesized kaolinite from raw diatomite and gibbsite gave higher than that of calcined diatomite and gibbsite.

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시료 중 잔류 항생제 분석 방법: I. 고상 시료 전처리 방법 (Determination of Antibiotic Residues: I. Extraction and Clean-up Methods for Solid Samples_A Review)

  • 김찬식;류홍덕;정유진;김용석;류덕희
    • 한국물환경학회지
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    • 제32권6호
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    • pp.600-627
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    • 2016
  • Korea is one of the countries with a large veterinary antibiotics market, although antimicrobial resistance in bacteria is becoming a serious issue in many countries. The Korean government started to take interest in estimating the effects of livestock manure on rivers and agricultural soils and in monitoring of heavy metals, organic pollutants and antibiotics in the ambient water and soil. In this paper, pre-treatment methods to separate the selected antibiotics from solid samples were reviewed. It is essential to select an efficient and appropriate procedure for pre-treatment due to the high proportion of proteins and organics in biosolid samples. Pre-treatment consists of extraction followed by clean-up. Initially, homogenized samples were extracted by sonication, mechanical agitation or pressurized liquid extraction with methanol/acetonitrile/water mixture under acidic/basic conditions depending on the compound. However, aminoglycosides and colistin were extracted with 5% trichloroacetic acid and HCl, respectively. Since the ${\beta}-lactams$ are easily decomposed in acidic and basic conditions, they were extracted in neutral pH. Filtration with a membrane (pore size, $0.2{\mu}m$) or solid phase extraction with HLB and methanol, as eluents, was normally applied for the clean-up. At least, three different pre-treatment procedures should be adopted to screen all the selected antibiotics in solid samples.

난백겔의 열안정성에 관한 연구 1, 가열온도와 시간, pH 및 NaCl농도가 난백겔의 열안정성에 미치는 영향 (Studies on Heat Stability of Egg Albumen Gel 1. Effects of Heating Time and Temperature, PH and NaCl Concentration on Heat Stability of Egg Albumen Gel)

  • 유익종;김창한;한석현;송계원
    • 한국가금학회지
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    • 제17권2호
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    • pp.127-133
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    • 1990
  • 가열온도와 시간, pH 및 NaCl 농도가 난백겔의 열안정성에 미치는 영향을 검토하기 위하여 가열처리 후 난백겔의 특성을 검토하였으며 그 결과는 다음과 같다. 난백을 90~$170^{\circ}C$까지 온도와 시간별 가열처리 한 결과. 110~$130^{\circ}C$ 영역에서 경도가 떨어졌으나 온도가 높아짐에 따라서 급격히 증가하였다. 응집성의 경우는 12$0^{\circ}C$까지 감소한 후 $130^{\circ}C$에서 급격히 증가하였다. 명도는 온도가 높아질수록 시간이 길어질수록 낮아졌으며 황색도는 높아졌다. 난백의 경도는 pH 7을 중심으로 산성영역에서는 고온처리가 높았으며 알카리영역에서 밝았다. 고온처리의 경우가 저온처리에 비해 산성영역에서는 보다 밝았으며 알카리 영역에서는 보다 어두웠다. 염의 첨가에 의해 난백의 경도는 저온처리 시에는 큰 변화가 없었으나 고온처리시 다소 증가하였고 응고집성은 저온처리시 서서히 증가하였으며 고온처리시 0.5%까지 급격히 증가하였다. 색택은 고온고리시 명도가 증가하였고 황색도는 감소하였으며 농도별 차이는 크지 않았다.

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새로운 광변색성 Spiroxazine색소의 합성과 특성 (Syntheses and Characterisitics of New Photochromic Spiroxazine Dyes)

  • 김성훈
    • 한국인쇄학회지
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    • 제12권1호
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    • pp.159-168
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    • 1994
  • The Aggregation State of thin vapor deposite Film made by Cyanine Dye were changed into Monomeric molecular state(M) and Dimeric Aggregation(D) at the acidic treatment, into reversible H-Aggregation (H) at alkalic gas or heat treatment. Photo-electric properties were higher H than D and M, this stats were effective in enhancing Photoelectric-conversion characteristic. Substitute group of Meso-Position being stronger electronic Donors, Electronic density is higher and Electric conductivity is enhanced, we confirmed Oscillator Strength calculated by PPP-Molecular Orbital Calculation and Absorption Spectra at solvent stats were agreed good.

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배추 엽록체의 광계II 활성에 미치는 구리이온의 영향 (Effect of Cupric Ion on the PSII Activity in Isolated Chinese Cabbage Chloroplasts)

  • 박인호
    • Journal of Plant Biology
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    • 제30권3호
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    • pp.181-187
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    • 1987
  • Copper inhibited PSII-mediated O2 evolution (H2OlongrightarrowDCIP, H2OlongrightarrowSiMo) but not PSImediated O2 uptake(DCIP. Asc.longrightarrowMV) in isolated Chinese cabbage chloroplasts. Copper toxicity on PSII-mediated O2 evolution was higher at alkaline condition than at acidic condition and was inhanced by light illumination after copper treatment. The increased toxicity by light illumination was not recovered by subsequent dark treatment. The inhibitory effect of copper on H2OlongrightarrowDCIP reaction was higher than that on H2OlongrightarrowSiMo reaction. This result suggests that there may be another inhibitory site of copper on PSII other than water oxidizing side of PSII.

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홍삼산성다당체 (RGAP)와 항암제의 병용투여에 의한 항암시너지 효과 (Anticancer Activities by Combined Treatment of Red Ginseng Acidic Polysaccharide (RGAP) and Anticancer Agents)

  • 곽이성;김영숙;신한재;송용범;박종대
    • Journal of Ginseng Research
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    • 제27권2호
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    • pp.47-51
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    • 2003
  • Sarcoma 180 암세포를 이식한 마우스에서 면역조절 작용을 갖는 홍삼산성다당체 (RGAP)를 항암제 cyclophosphamide (CY)와 병용투여한 결과, RGAP (100 mg/kg)와 CY (3 mg/kg)의 병용투여는 고용량의 CY (10 mg/kg) 단독투여보다 강한 항암작용을 나타내었다. 또한 RGAP (100 mg/kg)과 CY (10 또는 20 mg/kg)과의 병용투여는 CY의 단독투여보다 LL/2 폐종양을 보다 강하게 억제하였다. RGAP (100 mg/kg) 와 5-fluorouracil (5-FU) (2.5 mg/kg)을 병용투여하였을 때도 sarcoma 180 암세포를 이식한 마우스에서 5-FU 단독투여군에 비해 현저한 항암시너지 효과를 나타내었다. LL2 폐 종양에 미치는 결과에서도 RGAP (100 mg/kg)와 5-FU (5 또는 10 mg/kg) 병용투여군은 항암제 단독투여군보다 강한 항종양 효과를 나타내었다. 이상의 결과로부터 RGAP는 항암제 CY 또는 5-FU와 병용투여시 sarcoma 180 및 LL/2 폐종양에 대해서 저용량 항암제의 사용으로 고용량의 항암제 사용에서와 유사한 항암효과를 나타낼 수 있어서 항암효과를 극대화 시킬뿐만아니라 항암제 사용에 의한 면역독성과 같은 부작용을 경감시킬 수 있는 항암치료 보조제로서의 사용가능성을 제시하였다.