• Title/Summary/Keyword: acidic treatment

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Isolation and Differential Expression of an Acidic PR-1 cDNA Gene from Soybean Hypocotyls Infected with Phtophthora sojae f. sp. glycines

  • Kim, Choong-Seo;Yi, Seung-Youn;Lee, Yeon-Kyung;Hwang, Byung-Kook
    • The Plant Pathology Journal
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    • v.16 no.1
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    • pp.9-18
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    • 2000
  • Using differential display techniques, a new acidic pathogenesis-related (PR) protein-1 cDNA (GMPRla) gene was isolated from a cDNA library of soybean (Glycinemax L.Merr, cultivar Jangyup) hypocotyls infected by Phytophthora sojae f. sp. glycines. The 741 bp of fulllength GMPRla clone contains an open reading frame of 525 nucleotides encoding 174 amino acid residues (pI 4.23) with a putative signal peptide of 27 amino acids in the N-terminus. Predicted molecular weight of the protein is 18,767 Da. The deduced amino acid sequence of GMPRla has a high level of identity with PR-1 proteins from Brassica napus, Nicotiana tabacum, and Sambucus nigra. The GMPRla mRNA was more strongly expressed in the incompatible than the compatible interaction. The transcript accumulation was induced in the soybbean hypocotyls by treatment with ethephon or DL-$\beta$-amino-n-butyric acid, but not by wounding. In situ hybridization data showed that GMPRIa mRNAs were usually localized in the vascular bundle of hypocotyl tissues, especially phloem tissue. Differences between compatible and incompatible interactions in the timing of GMPRla mRNA accumulation were remarkable, but the spatial distribution of GMPRla mRNA was similar in both interactions. However, more GMPRla mRNA was accumulated in soybean hypocotyls at 6 and 24 h after inoculation.

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Treatment of dental erosion caused by intrinsic and extrinsic etiology: a case report (외인성 및 내인성 요인에 의한 치아부식에 대한 치료 증례)

  • Lee, Gyeong-Je;Jin, Soo-Yoon;Kim, Hee-Jung;Min, Jeong-Bum
    • Journal of Dental Rehabilitation and Applied Science
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    • v.33 no.1
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    • pp.42-46
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    • 2017
  • Dental erosion is defined as tooth structure loss by acidic chemical substance. It is caused by extrinsic factors such as acidic foods, drugs, and working environments, and also intrinsic factors such as gastric acid regurgitation in gastro-esophageal disorder or intensive vomiting in bulimia nervosa. These lesions can be treated with direct resin filling, laminate or full contour crown depending on the intensity of clinical problem. This is a case report about treatment of rare clinical case: labial erosion of anterior tooth caused by frequent intake of acidic fruit and palatal erosion of anterior tooth caused by intensive vomiting in bulimia nervosa.

Changes on Physical Properties of Chrome Leather by Treatment with Artificial Perspiration (크롬유혁의 인공땀액 처리에 의한 물성변화)

  • Yoon, Jeong Ah;Choi, Suk Chul;Lee, Yang Hun;Chun, Tae Il
    • Textile Coloration and Finishing
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    • v.7 no.4
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    • pp.25-32
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    • 1995
  • This study was carried out to examine the influences of fatigue and perspiration on the deterioration of chrome leather for shoes. The samples were treated with acidic and alkaline artificial perspirations and applied cyclic tensile stress, and the elastic recovery from fatigue strain, tensile strength and elongation, stiffness etc. by regions and directions were determined. The physical properties generally indicate considerable difference by regions and directions due to the thickness, entangling state, and arrangement direction of fiber buldles within the leather. By the treatment of artificial perspirations, the length, weight, strength, elastic recovery from fatigue of the lether are decreased and the thicknes, elongation, and stiffness are increased; and their extents by the alkaline perspiration are higher than those by the acidic one. The elastic recovery from fatigue of samples are decreased with increasing fatigue cylcle; and indicate regressive relations against the logarithm of the elapsed time, and the means of regression coefficients are 2.73 > 2.55 > 2.22 for samples treated by the acidic perspiration, untreated, and treated by the alkaline perspiration, respectively. The tensile strength is decreased and elogation is increased by fatigue, but the changes are diminished likely due to a rearrangment of fiber bundles with increasing fatigue cycle. And the samples become a little flexible immediately after fatigue; but stiffen again with elastic recovery from fatigue strain, consequently for some cases their stiffness after recovery are rather greater than those before fatigre.

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The Synthesis of Kaolinitic Clay Minerals from Domestic Diatomite by Hydrothermal Process (국내산 규조토의 수열반응에 의한 Kaolinite질 인공점토의 합성에 관한 연구)

  • 김남일;박계혁;정창주
    • Journal of the Korean Ceramic Society
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    • v.31 no.11
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    • pp.1401-1413
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    • 1994
  • The synthesis of kaolinite mineral from domestic diatomite for silica resource, commercial vailable gibbsite or alumina for alumina resource were made under various hydrothermal treatment, and the sythetic effect of acidic mineralizers, temperature treatment with time duration, particle size of alumina on formation of kaolinite mineral and the plastic properties of synthesized kaolinite were investigated. The various acidic mineralizers which are HCl, HNO3, H2SO4 and Oxalic acid were employed for hydrothermal reaction in the range of 0.01 mol/ι to 2 mol/ι concentration of each mineralizers. It was found that HCl in the level of 1 mol/ι solution produced highly yields of well-crystallized and platy form kaolinite mineral and gave the most effective extraction of iron oxide, compared to that of others, that HNO3 produced highly yield of kaolinite but lower extraction of iron oxide, that H2SO4 produced low yield of kaolinite and formed alunite mineral, and that oxialic acid formed spherical crystalline kaolinite and gave low extraction of iron oxide. Moreover, it showed that kaolinite minerals were well synthesized in a wide range of less than 2 mol/ι acids, but were poorly synthesized at more than 2 mol/ι acids. However, boehmite and kaolinite were coexistently formed in the temperature range of 18$0^{\circ}C$ and 20$0^{\circ}C$ when the calcined diatomite and gibbsite were involved. The well-ordered kaolinite mineral as a platy form was highly synthesized in the temperature range of 220 and 24$0^{\circ}C$, when the same marterials as above were used with treatment of 1 mol/ι HCl solution. The results also revealed that the size of crystalline platy form kaolinite, synthesized from alumina and calcined diatomite with treatment in 1 mol/ιHCl solution at 24$0^{\circ}C$, was much larger than that of gibbsite and calcined diatomite shown previously, and that kaolinite and corundum minerals were coexistently formed under any hydrothermal treatment conditions. The plasticity of synthesized kaolinite from under 2 ${\mu}{\textrm}{m}$ alumina and calcined diatomite was very poor, and that of the synthesized kaolinite from raw diatomite and gibbsite gave higher than that of calcined diatomite and gibbsite.

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Determination of Antibiotic Residues: I. Extraction and Clean-up Methods for Solid Samples_A Review (시료 중 잔류 항생제 분석 방법: I. 고상 시료 전처리 방법)

  • Kim, Chansik;Ryu, Hong-Duck;Chung, Eu Gene;Kim, Yongseok;Rhew, Doug Hee
    • Journal of Korean Society on Water Environment
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    • v.32 no.6
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    • pp.600-627
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    • 2016
  • Korea is one of the countries with a large veterinary antibiotics market, although antimicrobial resistance in bacteria is becoming a serious issue in many countries. The Korean government started to take interest in estimating the effects of livestock manure on rivers and agricultural soils and in monitoring of heavy metals, organic pollutants and antibiotics in the ambient water and soil. In this paper, pre-treatment methods to separate the selected antibiotics from solid samples were reviewed. It is essential to select an efficient and appropriate procedure for pre-treatment due to the high proportion of proteins and organics in biosolid samples. Pre-treatment consists of extraction followed by clean-up. Initially, homogenized samples were extracted by sonication, mechanical agitation or pressurized liquid extraction with methanol/acetonitrile/water mixture under acidic/basic conditions depending on the compound. However, aminoglycosides and colistin were extracted with 5% trichloroacetic acid and HCl, respectively. Since the ${\beta}-lactams$ are easily decomposed in acidic and basic conditions, they were extracted in neutral pH. Filtration with a membrane (pore size, $0.2{\mu}m$) or solid phase extraction with HLB and methanol, as eluents, was normally applied for the clean-up. At least, three different pre-treatment procedures should be adopted to screen all the selected antibiotics in solid samples.

Studies on Heat Stability of Egg Albumen Gel 1. Effects of Heating Time and Temperature, PH and NaCl Concentration on Heat Stability of Egg Albumen Gel (난백겔의 열안정성에 관한 연구 1, 가열온도와 시간, pH 및 NaCl농도가 난백겔의 열안정성에 미치는 영향)

  • 유익종;김창한;한석현;송계원
    • Korean Journal of Poultry Science
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    • v.17 no.2
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    • pp.127-133
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    • 1990
  • This study was undertaken to find out the effect of heating time and temperature, pH and NaCl concentration on the heat stability of egg albumen gel during heat treatment. With the transient decrease at 110-$130^{\circ}C$, hardness of heat-set albumen gel was increased as the heating temperature increased. The cohesiveness showed similar trend as well. The lightness was decreased while the yellowness was increased as the heating time and temperature increased. Heat-set albumen gel showed maximum hardness at pH 4.5-5.0 and pH 9.0 High heat treatment($120^{\circ}C$, 30min) showed higher hardness at alkaline range compared to low heat treatment($96^{\circ}C$, 30min.). Color of the albumen gel was relatively dark at acidic range and bright at alkaline range. High heat treatment caused darker albumen gel at alkaline range and brighter albumen gel at acidic range. The addition of NaCl increased hardness and cohesiveness of the albumen gel and improved the lightness after high heat treatment regardless of NaCl concentration.

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Syntheses and Characterisitics of New Photochromic Spiroxazine Dyes (새로운 광변색성 Spiroxazine색소의 합성과 특성)

  • 김성훈
    • Journal of the Korean Graphic Arts Communication Society
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    • v.12 no.1
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    • pp.159-168
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    • 1994
  • The Aggregation State of thin vapor deposite Film made by Cyanine Dye were changed into Monomeric molecular state(M) and Dimeric Aggregation(D) at the acidic treatment, into reversible H-Aggregation (H) at alkalic gas or heat treatment. Photo-electric properties were higher H than D and M, this stats were effective in enhancing Photoelectric-conversion characteristic. Substitute group of Meso-Position being stronger electronic Donors, Electronic density is higher and Electric conductivity is enhanced, we confirmed Oscillator Strength calculated by PPP-Molecular Orbital Calculation and Absorption Spectra at solvent stats were agreed good.

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Effect of Cupric Ion on the PSII Activity in Isolated Chinese Cabbage Chloroplasts (배추 엽록체의 광계II 활성에 미치는 구리이온의 영향)

  • 박인호
    • Journal of Plant Biology
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    • v.30 no.3
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    • pp.181-187
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    • 1987
  • Copper inhibited PSII-mediated O2 evolution (H2OlongrightarrowDCIP, H2OlongrightarrowSiMo) but not PSImediated O2 uptake(DCIP. Asc.longrightarrowMV) in isolated Chinese cabbage chloroplasts. Copper toxicity on PSII-mediated O2 evolution was higher at alkaline condition than at acidic condition and was inhanced by light illumination after copper treatment. The increased toxicity by light illumination was not recovered by subsequent dark treatment. The inhibitory effect of copper on H2OlongrightarrowDCIP reaction was higher than that on H2OlongrightarrowSiMo reaction. This result suggests that there may be another inhibitory site of copper on PSII other than water oxidizing side of PSII.

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Anticancer Activities by Combined Treatment of Red Ginseng Acidic Polysaccharide (RGAP) and Anticancer Agents (홍삼산성다당체 (RGAP)와 항암제의 병용투여에 의한 항암시너지 효과)

  • Kwak, Yi-Seong;Kim, Young-Sook;Shin, Han-Jae;Song, Yong-Bum;Park, Jong-Dae
    • Journal of Ginseng Research
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    • v.27 no.2
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    • pp.47-51
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    • 2003
  • Synergistic anticancer activities of red ginseng acidic polysaccharide (RGAS) showing immunomodulatory activity were evaluated by combined treatment with anticancer agents such as Cyclophosphamide (CY) or 5-Fluorouracil (5-FU) in experimental tumor models. The combined treatment of RGAP (100 mg/kg) and CY (3 mg/kg) exhibited 71% of survival rate in lift span of sarcoma 180-bearing mice, while single treatment of RGAP (100 mg/kg) and CY (3 or 10 mg/kg) exhibited 43, 14 and 43% of survival rates, respectively. In addition, when RGAP (100 mg/kg) was administered in combination with 5-FU (2.5 mg/kg) to sarcoma 180 tumor-bearing mice, higher survival rate was found when compared with RGAP or 5-FU treatment alone. Moreover, tumor weights in LL/2 lung carcinoma-bearing mice treated combined with RGAP (100 mg/kg) and 5-FU (5 or 10 mg/kg) was obviously decreased when compared with 5-FU alone. These results suggest that clinical trials of RGAP as an adjuvant in cancer chemotheraphy can be higly feasible.