• Title/Summary/Keyword: acidic pH

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A Method for Direct Application of Human Plasmin on a Dithiothreitol-containing Agarose Stacking Gel System

  • Choi, Nack-Shick;Chung, Dong-Min;Yoon, Kab-Seog;Maeng, Pil-Jae;Kim, Seung-Ho
    • BMB Reports
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    • v.38 no.6
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    • pp.763-765
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    • 2005
  • A new simplified procedure for identifying human plasmin was developed using a DTT copolymerized agarose stacking gel (ASG) system. Agarose (1%) was used for the stacking gel because DTT inhibits the polymerization of acrylamide. Human plasmin showed the lowest activity at pH 9.0. There was a similar catalytically active pattern observed under acidic conditions (pH 3.0) to that observed under alkaline conditions (pH 10.0 or 11.0). Using the ASG system, the primary structure of the heavy chain could be established at pH 3.0. This protein was found to consist of three fragments, 45 kDa, 23 kDa, and 13 kDa. These results showed that the heavy chain has a similar structure to the autolysed plasmin (Wu et al., 1987b) but there is a different start amino acid sequence of the N-termini.

A Study on the Formaldehyde Release from Durable Press Finished Rayon Fabrics (DP 가공된 레이온 직물의 포름알데하이드 방출에 관한 연구)

  • Yeo Sook-Young;Kim Eun-Ae
    • Journal of the Korean Society of Clothing and Textiles
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    • v.13 no.1 s.29
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    • pp.59-66
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    • 1989
  • This paper is concerned with formaldehyde release from durable press finished rayon fabrics. $100\%$ rayon fabrics were treated with 3 kinds of commercial N-methylol crosslinking agents using a pad-dry cure technique. Aqueous extractions of fabric samples were carried out at $40^{\circ}C$ under pH's of 4, 7, 10. Formaldehyde release was evaluated for the types of resins, catalyst concentrations and extraction conditions. Results indicated that the higher concentration of catalyst leads to the more fixation of resin on the fabric. Total formaldehyde released to the extract was decreased as the catalyst concentration increased. For the resin types, the amount of formaldehyde released was in the order of DMU>MDMDHEU>DMDHEU. Free formaldehyde content in the extract was in the order of pH10>pH4>pH7. This result proved that resins are least resistant to alkaline hydrolysis and the N-C bond cleavage under alkaline condition. Under acidic condition, however, N-methylol formaldehyde was accumulated before the release of free formaldehyde. This suggested the C·0 bond cleavalge to form carbonium - immonim intermediate.

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Abiotic Degradation Degradation of the Herbicide Oxadiazon in Water

  • Rahman Md. Mokhlesur;Park, Jong-Woo;Park, Man;Rhee In-Koo;Kim, Jang-Eok
    • Journal of Applied Biological Chemistry
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    • v.49 no.4
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    • pp.157-161
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    • 2006
  • The performance of abiotic degradation of oxadiazon was investigated by applying zerovalent iron(ZVI), potassium permanganate($KMnO_4$) and titanium dioxide($TiO_2$) in the contaminated water. Experimental conditions allowed the disappearance of oxadiazon in the abiotic system. The degradation of this herbicide was monitored in buffer solutions having pH 3, 5 and 7 in the presence of iron powder in which the maximum degradation rate was achieved at acidic condition(pH 3) by 2% of ZVI treatment. The oxidative degradation of oxadiazon was observed in aqueous solution by $KMnO_4$ at pH 3, 7 and 10 in which the highest disappearance rate was found at neutral pH when treated with 2% of $KMnO_4$. The catalytic degradation of oxadiazon in $TiO_2$ suspension was obtained under dark and UV irradiation conditions. UV irradiation enhanced the degradation of oxadiazon in aquatic system in the presence of $TiO_2$. Conclusively, the remediation strategy using these abiotic reagents could be applied to remove oxadiazon from the contaminated water.

The study on the formation of titanic acid by dehydration of TiCl4 (TiCl4 가수분해에 의한 titanic acid의 생성에 관한 연구)

  • Kim, Hern;Kim, Dae-Woong;Lee, Kyung-Hee;Baik, Woon-Phil
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.8 no.2
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    • pp.343-349
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    • 1998
  • The effect of pH on the dehydration reaction of $TiCL_4$ solution. KOH and HC1 were used as a accelerater and retarder in dehydration. Results are follow. Neutralization point is pH 7.4 in the system of $TiCL_4$- KOH and the production which is produced at acidic side is Ti -gel of poly metatitanic acid. The production which is produced at alkalic side is aligomer and crystalline potasium titanate is not detected.

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The Kinetics and Mechanism of Nucleophilic Addition of Mercaptan to a ${\beta}$-Nitrostyrene in Acidic Media(III) n-Propylmercaptan and tert-Butylmercaptan (酸性溶煤 속에서의 $\beta$-Mercaptan에 대한 親核性 添加反應에 관한 硏究 (III) n-Propylmercaptan 및 tert-Butylmercaptan 과의 반응)

  • Park, Ok-Hyun;Kim, Tae-Rin
    • Journal of the Korean Chemical Society
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    • v.12 no.4
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    • pp.177-183
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    • 1968
  • The rate equations for the nucleophilic addition reactions of n-propyl- and tert-buryl mercaptan to 3,4-methylenedioxy-$\beta$-nitrostyrene over wide pH range were obtained. From this equations, the rate constants for n-propyl-and tert-butylmercaptide ions at high pH were obtained numerically as $1.26{\times}10^8$ and $3.98{\times}10^6\;M^{-2},\;sec^{-1}$, and for n-propyl- and tert-butyl mercaptan at low pH, $7.07{\times}10^{-3}$ and $1.5{\times}10^3\;M^{-1},\;sec^{-1}$ respectively.

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The removal of Nitrate-nitrogen from ground water by electrodialysis (전기투석을 이용한 지하수 중의 질산성질소 제거)

  • Min, Ji-Hee;Kim, Han-Seung
    • Journal of Korean Society of Water and Wastewater
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    • v.22 no.3
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    • pp.307-314
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    • 2008
  • In this study, the effects of applied voltage, solution pH and coexistence of other ions such as sulfate ion (${SO_4}^{2-}$) and chloride ion ($Cl^-$) were investigated on the removal of nitrate-nitrogen ($NO_3{^-}-N$) from ground water by electrodialysis. The examined operating conditions were evaluated for optimizing the removal efficiency of $NO_3{^-}-N$. Real ground water samples taken from a rural area of Yongin city and artificial ones with components similar to the real ground water were tested for the study, which contained $NO_3{^-}-N$ concentration of 17mg/L that exceeds current drinking water quality standard of 10 mg/L. The increase in the removal rate of $NO_3{^-}-N$ was observed as the applied voltage increased from 5V to 30V, while no significant increase in the removal rate appeared at the applied voltage beyond 20V during a given operating time. The removal rate appeared to get lower at both acidic and basic condition, compared to neutral pH. Coexistence of of ${SO_4}^{2-}$and $Cl^-$ demanded much longer operating time to achieve a given removal rate or to meet a certain level of treated water concentration. When nitrate ion was combined with ${SO_4}^{2-}$and $Cl^-$, the removal rate was reduced by 4.29% and 10.83%, respectively.

Gas-Sensing Membrane Electrodes for the Determination of Dissolved Gases (I). Continuous-Automated Determination of Nitrite Ion Using Tubular PVC Membrane Type of pH Electrode (용해기체 분석용 기체 감응막 이온선택성 전극 (제 1 보). 관형 PVC 막 pH 전극을 이용한 아질산이온의 연속·자동화 정량)

  • Heung Lark Lee;Jong Hoon Yun
    • Journal of the Korean Chemical Society
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    • v.33 no.4
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    • pp.388-398
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    • 1989
  • A continuous-automated method for the determination of nitrite ion using gas-sensing membrane electrode was developed. The pH electrode of tubular PVC membrane type was used as a detector of this system. The slope of linear response of the electrode measured at optimum conditions for the continuous-automated determination of nitrite ion was 63.5 mV/decade. The concentration range of linear response and detection limit were 2.5 ${\times}10^{-4}{\sim}\;7.5{\times}10^{-2}$M and $8.0{\times}10^{-5}$M, respectively. This detection system was not only less interfering to acidic gas species than other methods but also less time consumable for determination.

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Quality Characteristics of Porridge Made from Rice Flour Processed into Rice Powder (쌀가루전용 품종으로 제조한 죽의 품질 특성)

  • Lee, Youn Ri;Song, Yu Ri;Kim, Ji Su;Jung, Hye Jin;Oh, Mi Reu
    • The Korean Journal of Food And Nutrition
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    • v.33 no.5
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    • pp.584-587
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    • 2020
  • The value of using rice flour processed into rice powder in preparing porridge was evaluated by measuring the moisture content, pH, total starch, reducing sugar, and color content. The moisture content of the rice flour and the pH of porridge made with rice flour were measured. Rice flour contained 77.55% moisture and the moisture content of the porridge made from rice flour processed into rice powder was 91.40 to 92.47%. The pH of rice flour porridge was acidic at 6.53 to 6.95. The color of the porridge was also measured. The L* values ranged from 82.62 to 97.55, the a* values ranged from -0.09 to 0.08, and the b* values ranged from -2.74 to 1.91.

Drug Release Control of Poloxamer-Poly(acrylic acid) Interpenetrating Polymer Networks (폴록사머-폴리아크릴산 IPNs의 약물 조절 방출)

  • Byun, Eun-Jung;Park, Joo-Ae;Lee, Seung-Jin;Kim, Kil-Soo
    • YAKHAK HOEJI
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    • v.41 no.1
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    • pp.22-29
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    • 1997
  • Poloxamer-poly (acrylic acid) (PAA) interpenetrating polymer networks (IPNs) were prepared via matrix polymerization of acrylic acid with poloxamer prepolymer. The equilibrium s welling of poloxamer/PAA IPNs was determined in various pH medium. The swelling of poloxamer/PAA IPNs was more affected by pH difference compared with the swelling of homo PAA gel due to protonation and deprotonation of the PAA network, followed by reversible formation and dissociation of the interpolymer complex due to hydrogen bonding between acidic hydrogens and ether oxygens. Nonionic/anionic/cationic drugs were incorporated into IPN matriceds as a model drug and their release behavior was studied. Nonionic, drug revealed release patterns depending solely on pH dependent swelling kinetics. In contrast, the release of ionic drugs was significantly affected by ionic drug-polymer interaction as well as the swelling kinetics.

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Growth Response to Acid Rain, Mg Deficiency and Al Surplus, and Amelioration of Al Toxicity by Humic Substances in Pitch Pine Seedlings

  • Joon-Ho Kim
    • Journal of Plant Biology
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    • v.37 no.3
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    • pp.301-308
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    • 1994
  • The individual and combined effects of acidic rain, Mg deficiency (-Mg) and Al surplus (+Al) on the growth of shoots and roots of pitch pine seedlings and the effect of humic substances (Lit) on Al toxicity were investigated. The growth of height and dry matter were not significantly less for pitch pine seedlings sprayed with simulated acid rain (SAR) of pH 3.5 than for those sprayed with SAR of pH 5.6. But treatments of Al and +Al-Mg in soil solution reduced the growth of seedlings in terms of height of shoots, and dry matter of shoots or roots. Effect of Mg deficiency on the growth of seedlings was apparent only when Al was treated simutaneously. The growth of seedlings, regardless of rain pH, decreased in the following order: control=-Mg>Lit+Al>+Al>+Al-Mg. Treatments of Al and +Al-Mg in soil solution reduced the total length of secondary and teritary roots of seedlings regardless of rain pH, and decreased in the following order: the primary root

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