• 한국식생활문화학회지
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• 제21권3호
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• pp.319-324
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• 2006

묵은 배추김치의 휘발성 성분들을 고진공승화법을 이용하여 분리, 농축하였으며, GC-MS로 분석하였다. 총 62가지의 휘발성 성분들이 검출되었으며, 7개의 sulfur-containing compounds, 8개의 terpenes, 5개의 esters, 8개의 acids, 15개의 alcohols, 2개의 nitriles, 2개의 ketones, 11개의 aliphatic hydrocarbons, 4개의 miscellaneous compounds등으로 구성되어 있었다. 이 중 특히, acids인 acetic acid와 butanoic acid가 높은 함량을 나타내었다. 묵은 배추김치에서 향기활성성분을 규명하기 위해 고진공승화법을 이용하여 휘발성 성분들을 분리 및 농축 후 향 추출물 희석분석법으로 flavor dilution(FD) factor($Log_3FD$)를 구하였으며, GC-MS 및 GC-Olfactometry(GC-O)법으로 휘발성 성분들을 동정하였다. 이 결과 묵은 배추김치에서 총 16종의 화합물이 검출되었다. 이중 시큼한 냄새, 군덕내의 특성을 지닌 acetic acid, butanoic acid가 높은 FD factor를 보였으며, acids외에 높은 (FD > 9)를 나타내는 성분들로는 linalool, 2-vinyl-4H-1,3-dithin 등이 있었다.

• 식품과학과 산업
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• 제50권4호
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• pp.24-49
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• 2017

Volatile flavor compounds of cheonggukjang and doenjang, which are the most representative Korean soybean fermented foods, were compiled throughout literature review. Total of 225 and 404 volatile flavor compounds were found in cheonggukjang and doenjang, respectively. The most characteristic volatile flavor compounds in cheonggukjang are thought to be pyrazine compounds. In addition, acids, such as 2-methyl propanoic acid, butanoic acid, 2-methyl butanoic acid, and 3-methyl butanoic acid, contribute to aroma characteristics of cheonggukjang. On the other hand, ester compounds are the most predominant volatile flavor compounds in doenjang. Ninety six ester compounds were detected in doenjang while 22 ester compounds were identified in cheonggukjang. Pyrazine compounds and acids also play an important role in the flavor of doenjang. Compilation of volatile flavor compounds from cheonggukjang and doenjang will provide basic information to food industry to understand and improve aroma characteristics of cheonggukjang and doenjang.

• The Korean Journal of Ecology
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• 제19권4호
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• pp.329-340
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• 1996

The extracts of selected plants and analyzed phenolic compounds were used to study the effects of alleloKDICicals on seed germination and seedling growth. HPLC analysis of the aqueous extracts of seven species identified 15 phenolic compounds including caffeic acid. Among them, protocatechuic acid was detected at 65.87ppm and 6.84ppm, in Erigeron canadensis and Pinus rigida, respectively. And the extract of P. rigida showed the strongest inhibitory effect on seed germination. The extract of P. rigida leaves significantly inhibited germination and radicle growth of Raphanus sativus var. hortensis for. acanthiformis in direct proportion to concentration. However, germination of Cassia mimosoides var. nomame was stimulated by the treated extracts at the same concentrations, but root growth was inhibited at high concentrations. Except chlorogenic acid, eleven of the twelve phenolic compounds inhibited the germination of R. sativus var. hortensis for. acanthiformis. In the case of C. mimosoides var. nomame, some phenolic compounds such as chlorogenic acid, vanillic acid, protocatechuic acid, ferulic acid, gallic acid and ${\rho}-coumaric$ acid stimulated germination, while the others reduced it.

• 생약학회지
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• 제30권3호
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• pp.345-349
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• 1999

Five compounds were isolated from the BuOH fraction of Polygonum bistorta (Polygonaceae). On the basis of spectral data, these compounds were established as caffeic acid, quercimeritrin, avicularin, gallic acid and protocatechuic acid. The inhibitory activies on $3{\alpha}-hydroxysteroid$ dehydrogenase $(3\;{\alpha}-HSD)$ of isolated compounds were compared. $IC_{50}$ value of isolated compounds were $133.57\;{\mu}g/ml\;(caffeic\;acid)$, $89.1\;{\mu}g/ml\;(quercimeritrin)$, $189.85\;{\mu}g/ml\;(avicularin)$, $140.69\;{\mu}g/ml\;(gallic\;acid)$ and $165.27\;{\mu}g/ml\;(protocatechuic\;acid)$ respectively. Although all compounds showed lower inhibition activities than BuOH fraction $(IC_{50}<50\;{\mu}g/ml)$ of Polygonum bistorta, it showed higher inhibition activities than aspirin $(IC_{50}\;246.81\;{\mu}g/ml)$.

• Archives of Pharmacal Research
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• 제14권1호
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• pp.48-51
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• 1991

Amides from amino acid ester and nalidixic acid were synthesized. The solubility characteristics and partition coefficient of the compounds were studied. The hydrolysis of the compounds was studied in the simulated gastric fluid and simulated intestinal fluid. Some compounds showed better antibacterial activity than nalidixic acid.

• 한국식품과학회지
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• 제28권2호
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• pp.232-239
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• 1996

천연에 광범위하게 존재하고 있는 각종 폐놀성 화합물은 니트로화 반응의 저해제로 관여하거나 항산화제로 작용하는 것으로 알려져 있어 본 연구에서는 이들 각종 페놀성 화합물의 아질산염 소거작용과 전자공여 작용 유무를 알아보기 위하여 이들 화합들의 농도를 $0.1mM{\sim}6mM$용액을 조제한 후, 이 액 일정량을 이용해 아질산염 소거작용과 전자공여 작용을 비교하였다. 아질산염 소거율에서 phenolic acid류는 높은 활성을 보였으며, 일부는 pH 1.2, 3.0에서 flavonoids보다 높은 활성을 나타내었다. 전자공여능에서 phenolic acid류 가운데 hydroxybenzoic acids는 gallic acid> gentisic acid> syringic acid> protocatechuic acid> salicylic acid> vanillic acid> benBoic acid>p-hydroxy benzoic acid 순으로 나타났으며, hydroxycinnarnic acids가운데 전자공여능의 크기는 hydrocaffeic acid> caffeic acid> ferulic acid> p-coumaric acid>trans-cinnamic acid 순으로 나타났고, flavonoids 가운데에서는 (+)catechin> rutin> quercetin>naringin=hesperidin 순으로 나타났다. 또한 tannic acid를 포함한 기타 phenol성 화합물에 없어서도 높은 전자공여능을 나타내었다.

• Natural Product Sciences
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• 제23권2호
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• pp.75-83
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• 2017

The optimization and microwave assisted extraction of stem bark of Terminalia arjuna, quantitative estimation of the marker compounds arjunic acid and arjunolic acid using HPTLC and the evaluation of free radical scavenging activity has been performed in this study. The central composite design was used for optimization and the values of parameters for optimized batch of microwave assisted extraction were 1000 W (Power), 3 minutes (Time) and 1/120 (Solid/solvent ratio). The solvent system to carry out the HPTLC was toluene: acetic acid: ethyl acetate (5: 5: 0.5) and quantitative estimation was done using standard equations obtained from the marker compounds. The in-vitro free radical scavenging activity was performed spectrophotometrically using ascorbic acid as standard. The value of estimated percentage yield of arjunic acid and arjunolic acid was 1.42% and 1.52% which upon experimentation was obtained as 1.38% and 1.51% respectively. The DPPH assay of the different batches of microwave assisted extraction and marker compounds taken suggested that the marker compounds arjunic acid and the arjunolic acid were responsible for the free radical scavenging activity as the batch having the maximum percentage yield of the marker compounds showed best free radical scavenging effect as compared to standard ascorbic acid. The $IC_{50}$ value of the optimized batch was found to be 24.72 while that of the standard ascorbic acid was 29.83. Hence, the yield of arjunic acid and arjunolic acid has direct correlation with the free radical scavenging activity of stem bark extract of Terminalia arjuna and have potential to serve as active lead compounds for free radical scavenging activity.

• Archives of Pharmacal Research
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• 제21권6호
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• pp.723-728
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• 1998

The chemical behaviours of 4-methyl-2-oxo-2H-benzopyran-7-yl oxoacetyl hydrazine (2) towards some different reagents such as anhydride compounds, aromatic aldehydes, carb on disulphide, and nitrous acid yielded the corresponding pathalazine derivatives (3, 4, 5), hydrazone derivative (6), 1,3,4-oxadiazole derivative (7, 8, 9) and acid azide (10) respectively. Treatmen of 10 with absolute alcohols, amines and ethyl amino acid ester gave the corresponding carbamate derivative (11), substituted urea derivative (12) and ethyl substituted alkyl acetate (13) respectively. The biological activity of some synthesized compounds was evaluated.

• 한국연초학회지
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• 제22권2호
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• pp.107-113
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• 2000

This study was carried out to investigate the changes in composition of the non-volatile organic acid, fatty acid and polyphenolic compounds during air-curing in burley tobacco leaves, and the effect of curing methods on the contents in air-cured leaves. The air-cured variety, (Nicotiana tabacum cv KB108) was normally grown at the Chonju tobacco experiment station in 1998. Plants designated for the each curing methods were harvested on the same date, and the ripe leaves for primed curing were harvested at the tenth leaf position from the top on the stalk. The major compounds of non-volatile organic acid and fatty acid were malic, citric, oxalic, palmitic, and linolenic acid. The concentrations of malic acid, unsaturated fatty acids, chlorogenic acid and rutin in cured leaves were remarkably decreased during curing, while citric acid was increased. The changes of these compounds showed the similar pattern during both primed and stalk curing. In connection with curing methods, the contents of malic, linoleic and linolenic acid were higher in excessive cured leaves than those in primed cured or stalk cured leaves, while the content of citric acid was lower in excessive cured leaves than that in primed cured or stalk cured leaves.

• 한국연초학회지
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• 제22권1호
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• pp.84-90
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• 2000

This study was conducted to determine the acid and phenol compounds in smoke total particulate matter(TPM) by the different tobacco variety, and grade of tobacco leaves. Sixteen kinds of tobacco leaves which were flue-curd, burley, orient, reconstituted tobacco, expanded stem, and expanded cut tobacco, were selected for this study. After collecting a TPM by using smoking machine, the concentration of TPM components was analyzed by GC. Acid components of TPM of mainstream smoke were different from the variety and grade. The order of the highest concentration of acid compounds in TPM was flue-cured > orient> burley> expanded cut tobacco> reconstituted tobacco> expanded stem. Though lactic acid and glycolic acid concentrations in flue-cured tobacco were twice higher than those in burley tobacco, the contents of 2-furoic acid and 3,4-dihydroxy butanoic acid in burley tobacco were higher than those in flue-cured tobacco. The content of phenolic compounds in the high grade and thick leaves was higher than that in other tobacco leaves. Phenol and catechol compounds in burley CD3W-2 revealed the least value in concentration among the samples tested. Pyrocatechol and hydroquinone concentrations in flue-cured tobacco were 2-3 times higher than those in burley and orient tobacco.