• Title/Summary/Keyword: acid chloride

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The Effect of Metal Compounds on Phospholipid Biosynthesis and Fatty Acid Composition in Escherichia coli and Bacillus subtilis (Escherichia coli와 Bacillus subtilis의 당지질 생합성과 지방산 조성에 미치는 여러가지 금속화합물의 영향)

  • 이소은;이종삼
    • Journal of Environmental Health Sciences
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    • v.21 no.2
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    • pp.54-67
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    • 1995
  • The biosynthesis of galactolipid and galactose and their composition of fatty acid in E. coli and B. subtilis treated ] with copper chloride (10 ppm), nickel chloride (50 ppm), manganese chloride (100 ppm) during the culture were analyzed. The contents of MGDG, DGDG and total lipids in treatment with metal compounds were lower to compared with the control. In E. coli, the major fatty acid unitized for biosyntheis of MGDG were palimitic acid (ave. 36.87%) and linolenic acid (ave. 14.79%) in control. In MGDG, the major fatty acids were utilized for palmitic acid (ave. 20.00%) and myristic acid (ave. 7.32%) in treatment with copper chloride, lauric acid (ave. 11.71%) and linolenic acid (ave. 11.06%) in manganese chloride treatment. And in nickel chloride treatment, it was palmitic acid (ave. 36.16%) and oleic acid (ave. 6.43%) were use in MGDG formation. In DGDG, in copper chloride treatment, it was lauric acid (ave. 19.41%) and oleic acid (ave. 9.95%) in biosynthesis of galactolipid. and in treatment with nickel chloride linolenic acid (ave. 15.39%) and linoleic acid (ave. 13.51%), in manganese chloride treatment palmitic acid (ave. 29.76%) and palmitoleic acid (ave. 11.35%) were used in DGDG formation. In B. subtilis, the major fatty acids utilized for biosynthesis of galactolipid was palmitic acid (ave. 30.86%) and linolenic acid (ave. 8.36%) in control. Otherwise, in MGDG, the major fatty acids were utilized for palmitic acid (ave. 28.92%) and stearic acid (ave. 13.25%) in treatment with copper chloride, and palmitic acid (ave. 15.73%) and lauric acid (ave. 11.88%) in manganese chloride treatment. It was continned that nickel chloride treatment was palmitic acid (ave. 35.16%) and palmitoleic acid (ave. 12.47%). The major fatty acids in DGDG were utilized for palmitic acid(ave. 34.19%) and linoleic acid (ave. 17.45%) in copper chloride treatment, and lauric acid (ave. 11.16%) and myrisitic acid (ave. 8.65%) in manganese chloride treatment. In treatment with nickel chloride, it was palmitoleic acid (ave. 10.30%) and myristic acid (ave. 7.81%) were used galactolipid formation.

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The Effects of Metal Compounds on the Phospholipid Metabolism in Bacillus subtilis;

  • Ma, Hye-Young;Jung, Kyung-Suk;Jang, Jae-Seon;Lee, Chong-Sam
    • Journal of Environmental Health Sciences
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    • v.23 no.2
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    • pp.1-11
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    • 1997
  • The synthesis of phospholipid and the composition of fatty acid in B. subtills treated with copper chloride (10 ppm), manganese chloride (100 ppm), and nickel chloride (50 ppm) during the culture were analyzed to compare with the control. The levels of growth, total lipid, phosphatidylethanolamine(PE), phosphatidylcholine(PC), phosphatidylglycerol(PG), and cardiolipin(CL) in B. subtilis treated with copper chloride were decreased predominantly. But, the biosynthesis of phosphatidylinositol(PI) was not affected by the metal compounds. The major fatty acids utilized for the formation of phospholipid were palmitic acid(average 19.00%) and stearic acid(average 9.88%) in the control. In the copper chloride treatment, however, palmitic acid (average 17.35%) and oleic acid(average 15.99%) made use of the major fatty acid during the biosynthesis of phospholipids. It was showed that oleic acid(average 17.87%) and stearic acid (average 13.78%) in thee manganese chloride treatment, and palmitic acid(average 15.00%) and myristic acid(average 14.24%) in the nickel chloride treatment were utilized.

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Studies on the synthesis and antitubercular activity of acyl derivatives of isonicotinic acid hydrazide (Isonicotinic acid hydrazide의 acyl 유도체 합성및 항균작용에 관한 연구)

  • 고현기
    • YAKHAK HOEJI
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    • v.13 no.1
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    • pp.43-46
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    • 1969
  • Five new acyl derivatives of isonicotinic acid hydrazide such as N-(2,4-dichlorophenoxyacetyl)-isoniotinic acid hydrazide (I), N-(p-nitrobenzoyl)-isonicotinic acid hydrazide (II), N-benzoylisonicotinic acid hydrazide (III), N-furoylisonicotinic acid hydrazide (IV) and N-(p-aminobenzoyl)-isonicotinic acid hydrazide (V) were synthesized. They were obtained by the action of 2,4-dichlorophenylacetyl chloride, p-nitrobenzoyl chloride, benzoyl chloride, furoyl chloride and p-aminobenzoyl chloride with isonicotinic acid hydrazide in pyridine solution. Evaluated for their in vitro antitubercular activity against Mycobaterium tuberculosis H$_{37}$ R$_{\upsilon}$ N-furoylisonicotinic acid hydrazide (IV) showed antitubercular activity at 1${\gamma}$/ml.

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Growth Inhibitory Effects of Chloride Salts and Organic Acid Salts Against Food-Borne Microorganisms (Chloride염 및 유기산 칼슘염의 식중독 미생물에 대한 증식 억제 효과)

  • 이나영;김용석;신동화
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.32 no.8
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    • pp.1233-1238
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    • 2003
  • The growth inhibitory effects of chloride salts and organic acid salts against six food-borne microorganisms (Bacillus cereus ATCC 11778, Escherichia coli O157:H7 ATCC 43894, Listeria monocytogenes ATCC 19111, Salmonella Typhimurium ATCC 14028, Staphylococcus aureus ATCC 25923, Vibrio parahaemolyticus ATCC 17802) were determined using Bioscreen C in broth medium. The growth inhibitory concentrations of sodium chloride and potassium chloride on B. cereus were 7 and 9%, respectively. E. coli O157:H7 and S. aureus were inhibited by treatment of 3% calcium chloride. Magnesium chloride showed growth inhibitory effect on B. cereus, S. Typhimurium, and S. aureus at 5%. The order of growth inhibition effects by organic acid salts was calcium propionate>calcium acetate>calcium lactate. Calcium chloride (3%) with 0.01% lactic acid showed strong inhibition on the growth of S. Typhimurium and exhibited stronger growth inhibition than calcium chloride alone (5%). We concluded that calcium chloride and calcium propionate had strong growth inhibitory activities and that calcium chloride and sodium chloride in combination with lactic acid had stronger inhibitory activities than that of chloride salts alone.

Phospholipid and Fatty Acid Metabolism in Escherichia coli-On the Effects of Various Metal Compounds

  • Ma, Hae-Young;Choi, Jong-Hak;Lee, Chong-Sam
    • Journal of Environmental Health Sciences
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    • v.22 no.2
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    • pp.114-123
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    • 1996
  • Escherichia coli에 copper chloride 50 ppm, manganese chloride 100 ppm, nickel chloride 100 ppm을 각각 처리하여 세포를 배양하는 동안에 이들 세포에서 일어나는 인지질 생합성 및 지방산 조성의 변화를 대조구와 비교하여 분석하였다. 세포의 생장과 total lipid, phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol, cardiolipin은 대조구조에 비해 금속 화합물 처리구에서 저해되었는데 nickel chloride가 가장 큰 억제 효과를 나타내었다. 그러나 phosphatidylinositol은 금속화합물의 영향을 받지 않았다. 인지질 생합성에 이용된 주요 지방산은 대조구는 palmitic acid(평균 25.47%)와 palmitoleic acid(평균 12.27%)가 인지질 생합성에 도입되었고 copper chloride 처리구는 palmitic acid(평균 30.13%)와 stearic acid(평균 9.12%)로 나타났다. manganese chloride 처리구와 nickel chloride 처리구에서는 모두 palmitic acid(평균 24.16%, 평균 21.77%)와 linoleic acid(평균 9.48%, 평균 11.88%)가 인지질 생합성에 이용된 주요 지방산으로 분석되었다.

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ClC Chloride Channels in Gram-Negative Bacteria and Its Role in the Acid Resistance Systems

  • Minjeong Kim;Nakjun Choi;Eunna Choi;Eun-Jin Lee
    • Journal of Microbiology and Biotechnology
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    • v.33 no.7
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    • pp.857-863
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    • 2023
  • Pathogenic bacteria that colonize the human intestinal tract have evolved strategies to overcome acidic conditions when they pass through the gastrointestinal tract. Amino acid-mediated acid resistance systems are effective survival strategies in a stomach that is full of amino acid substrate. The amino acid antiporter, amino acid decarboxylase, and ClC chloride antiporter are all engaged in these systems, and each one plays a role in protecting against or adapting to the acidic environment. The ClC chloride antiporter, a member of the ClC channel family, eliminates negatively charged intracellular chloride ions to avoid inner membrane hyperpolarization as an electrical shunt of the acid resistance system. In this review, we will discuss the structure and function of the prokaryotic ClC chloride antiporter of amino acid-mediated acid resistance system.

Effect of Browning Inhibitors on Quality Property of Fresh-Cut Strawberries (갈변억제제 처리가 신선 절단 딸기의 품질 특성에 미치는 영향)

  • Chung, Hun-Sik;Moon, Kwang-Deog
    • Food Science and Preservation
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    • v.19 no.1
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    • pp.26-30
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    • 2012
  • The effect of browning inhibitors on quality properties of fresh-cut strawberries was investigated. Half-cut strawberries were treated with dipping solutions alone and with combinations of 1% ascorbic acid, 1% citric acid, 1% sodium chloride, and 1% magnesium chloride, were packaged with low density polyethylene bags, and were kept for 24 hours at $4^{\circ}C$. The levels of oxygen and carbon dioxide in the packages of the samples treated with the combined solutions were the highest and the lowest, respectively. The loss of L value and flesh firmness of fresh-cut strawberries was retarded by the combined solutions than by the dipping solutions alone. The soluble solids and pH were not affected by the browning inhibitor. These results suggest that mixture of ascorbic acid, citric acid, sodium chloride, magnesium chloride may prevent browning and softening of fresh-cut strawberries.

Synthesis of 2-acetyl 11-keto-.DELTA.12;13 oleanolic ketol acetate and other derivatives (2-acrtyl-11-keto-.DELTA.12;13-oleanolic ketol acetate급 기타 유도체의 합성)

  • 김경호
    • YAKHAK HOEJI
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    • v.6 no.1
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    • pp.25-27
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    • 1962
  • The derivative of glycyrrhetinic acid which have similar effects of desoxycorticosterone acetate was prepared glycyrrhetinyl chloride by the chlorination with thionyl chloride at low temperature. 2-Acetyl-11-keto-.DELTA. : 13 oleanolic ketol acetate (30) was synthesized by diazotating glycyrrhetinyl chloride with diazomethan and the acetylation with anhydro acetic acid. 2-Acetyl-.DELTA.12 : 13 oleanolic ketol acetate (28) was synthesized by chlorinating oleanolic acid with thionyl chloride, and by diazotatating oleanolyl chloride with diazomethan and the acetylation with anhydroacetic acid.

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Antimicrobial Effect of Calcium Chloride Alone and Combined with Lactic Acid Injected into Chicken Breast Meat

  • Alahakoon, Amali U.;Jayasena, Dinesh D.;Jung, Samooel;Kim, Hyun Joo;Kim, Sun Hyo;Jo, Cheorun
    • Food Science of Animal Resources
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    • v.34 no.2
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    • pp.221-229
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    • 2014
  • Chicken breast meat was injected with calcium chloride alone and in combination with lactic acid (0.01% and 0.002%, respectively). The inhibitory effects of the treatments on microbial growth were determined in the injected chicken breast meat stored at $4^{\circ}C$ under aerobic packaging condition for 0, 3, and 7 d. Calcium chloride combined with 0.002% and 0.01% lactic acid reduced microbial counts by 0.14 and 1.08 Log CFU/g, respectively, however, calcium chloride alone was unable to inhibit microbial growth. Calcium chloride combined with 0.01% lactic acid was the most effective antimicrobial treatment and resulted in the highest initial redness value. Calcium chloride alone and combined with lactic acid suppressed changes in pH and the Hunter color values during storage. However, injection of calcium chloride and lactic acid had adverse effects on lipid oxidation and sensory characteristics. The higher TBARS values were observed in samples treated with calcium chloride and lactic acid when compared to control over the storage period. Addition of calcium chloride and lactic acid resulted in lower sensory scores for parameters tested, except odor and color, compared to control samples. Therefore, the formulation should be improved in order to overcome such defects prior to industrial application.

The Study of Textural Characteristics of Soybean Curd Prepared with various Coagulants (각종응고제에 따른 두부의 Texture 특성에 관한 연구)

  • 문수재
    • Journal of the Korean Home Economics Association
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    • v.17 no.1
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    • pp.12-19
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    • 1979
  • Five kinds of soybean curd were propared with five coagulants, such as, calcium sulfate, calcium chloride, magnesium chloride, glucono delta lactone and acetic acid. The products were evaluated by the sensory and objective examination. Optimal concentrations of each coagulant were determined. Soybean curd preparation was also standardized. The textural characteristics of the five soybean curds which were made by the standard recipe were measured by a Texturometer and a Penetrometer. The results were as follows : 1. From the proliminary study, the optimal concentration of coagulants for the soybean curd preparation, as determined by the sensory evaluation was 1.84% of calcium sulfute, 1.05% of calcium chloride. 1.84% of calcium sulfute, 1.05% of calcium chloride. 1.84% of magnesium chloride, 1.97% of glucono delta lactone and 0.48% 11of acetic acid. 2. As the result of the sensory evaluation, the most acceptable soybean curd was determined to be one with acetic acid. Next, in order of accetability , were magnesium chloride, calcium chloride, glucono delta lactone, calcium sulfate soybean curds and commerical soybean curd. 3. Through the objective examination of the five soybean curds by a Texturometer and a Penetrometer, it was found out that, calcium sulfate soybean curd was the hardest and the hardness decreased in order of glucono delta lactone, magnesium chloride, calcium chloride, and acetic acid soybean curd. Acetic acid soybean curd, the most acceptable , was 0.47 TU ; and calcium sulfate soybean curd, the least acceptable, was 1.73 TU.

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