• Environmental Analysis Health and Toxicology
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• v.16 no.1
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• pp.9-16
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• 2001

It is known that alcoholics have significantly lower mitochondrial aldehyde dehydrogenase (ALDH)s'activity than do normal subjects or nonalcoholics with liver disease. However, there are only few reports that explain the reasons behind this reduction of ALDHs'activities. In this study, ALDH activity is inhibited by acetaldehyde, a substrate for ALDH However, the addition of glutathione (GSH) protected ALDH activities against the inhibitory effects of acetaldehyde in vitro. Furthermore, when GSH depletion is induced using diethyl maleate (DEM) in rats by 24% in cytosol and 43% in mitochondria, ALDH activities were also depressed by 31% and 63%, respectively compared to non-treated rats without significant reductions in other hepatic enzymes. These results suggest that ALDHs'activities are closely related to the concentration of acetaldehyde and/or cellular GSH contents . Therefore in alcoholic liver disease, increased productions of acetaldehyde and decreased contents of mitochondrial GSH may involved in the depression of ALDHs'activities.

• Journal of Environmental Science International
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• v.19 no.6
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• pp.671-680
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• 2010

The present study evaluated residential exposure to atmospheric formaldehyde and acetaldehyde according to distance from the a dyeing industry complex (DIC). This purpose was achieved by measuring concurrently the outdoor air concentrations in residences near the DIC and a certain distance away, plus the outdoor air concentrations at two industrial areas within the DIC boundary. Formaldehyde concentrations (median values of 24.3 and $22.5{mu}g/m^3$ in IS1 and IS2, respectively) were higher than acetaldehyde concentrations (median values of 7.4 and $7.3{mu}g/m^3$ in IS1 and IS2, respectively) at both sites. However, there was no significant difference in the industrial outdoor air concentrations of both formaldehyde and acetaldehyde between the two sites. In addition, the median formaldehyde concentration from the residential site near the DIC (RS1) was about 1.5 times higher than that from the residential site far away from the DIC(RS2), and the median acetaldehyde concentration from RS1 was about 1.3 times higher than that from RS2. It is noteworthy that the mean or median risk as well as these maximum risks are well above the USEPA's permissible risk level of $10^{-6}$ from environmental exposure. This suggests that appropriate management for formaldehyde and acetaldehyde is necessary in order to decrease risk of the residents of study areas, regardless of the distance from the DIC.

• The Korea Journal of Herbology
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• v.34 no.1
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• pp.67-74
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• 2019

Objectives : Alcohol hangover is a common phenomenon which basically occurs after heavy drinking. Moreover, heavy alcohol consumption leads to acute and chronic diseases. We investigated the effect of herbal mixture (SJ) on alcohol metabolism in serum or/and liver. Methods : 5W-old Sprague Dawley rats were used for the study. To overnight fasted rats, 0.9% saline or SJ extract was administrated per os before alcohol treatment. Then, 40% alcohol was orally administrated to all rats in 30 mins. Ethanol, acetaldehyde concentrations, alcohol dehydrogenase (ADH), acetaldehyde dehydrogenase (ALDH) activities were measured by assay kits. Aspartate aminotransferase (AST) and alanine transaminase (ALT) were measured by analyzer. Results : Ethanol and acetaldehyde concentrations lowered in SJ groups compared with CON group. Especially, acetaldehyde concentration significantly decreased in SJ groups compared with CON group. AST and ALT levels tended to increase in SJ groups compared with CON group but there was no significant difference between CON group and SJ groups. ADH activity in serum was higher in SJ groups than CON group but no significant difference in liver. ALDH activity in both serum and liver showed significantly increased in SJ groups compared with CON group. Conclusions : Treatment of SJ extract showed not only reducing concentration of alcohol and acetaldehyde but also increasing activities of ADH and ALDH. These results suggest SJ may influence in alcohol metabolism via control of metabolic enzyme activities and metabolite. Therefore, SJ, herbal mixture, might have a function of preventing hangover after drinking alcohol.

• Preventive Nutrition and Food Science
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• v.6 no.1
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• pp.62-65
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• 2001

Persimmon has been known to help alcohol intoxication in Korea for a long time and is prepared as a processed food. The effect of dried persimmon snacks on alcohol metabolism was determined in vivo. Eight Korean men (25~27 years old) were administered 3.5 mL/kg of alcohol (22.5%, w/v) with or without a dried persimmon snack (2g/kg). The levels of alcohol in the blood and of acetaldehyde in the blood and the urine were determined by alcoholmeter and assay using alcohol dehydrogenase and HPLC, respectively. All subjects showed decreased levels of alcohol in blood, and six subjects showed a decrease of alcohol in urine after consumption of ethanol with dried persimmon snacks. Concentration of acetaldehyde in the blood an urine decreased significantly in three and five of the eight subjects, respectively. Reduction was more significant for alcohol than for alcohol than for acetaldehyde with administration of ethanol and dried persimmon snacks. This in vivo result suggests that dried persimmon snacks are effective in decreasing th concentration of alcohol after alcohol intake.

• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.5
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• pp.954-958
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• 2001

Perismmon has been consumed for long times in Korea and used as a drug for a long time in Korea, It was known to help alcohol intoxication. Ingested alcohol is metabilized by alcohol dehydrogenease and acetaldehyde dehydrogenase in liver. Alcohol dehydrogenease activator and acetaldehyde dehydrogenase activator(ALDHA) was detercted in persimmon. The oncentration of ALDHA was determined and compared in different havesting time, species, and available processed foods. The level of ALDHA was highest in persimmon (Fuyu) harvested in November. Lower ALDHA activities were found in its processed foods. Persimmon and its processed foods are expected to be effective in decreasing the concentration of alcohol and acetaldehyde after alcohol intake.

• Korean Chemical Engineering Research
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• v.54 no.1
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• pp.22-35
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• 2016

In this study CALPUFF modeling was performed to establish a correlation between regions of frequent civil odor complaints near Gumi national industrial complex and odor-emission facilities of synthetic fiber manufacturers in the same area as main acetaldehyde-emission point sources. As a result of the CALPUFF modeling, the maximum concentration of acetaldehyde in Gumi national industrial complex was reduced from O ($10^{-5}g/m^3$) to O ($10^{-6}g/m^3$) upon improving emission facilities of T company so that the maximum concentrations of acetaldehyde frequently appeared in complex 3. In addition, the predicted range of the maximum acetaldehyde concentration in Gumi national industrial complex was also improved in comparison with that prior to improving emission facilities of T company. These maximum concentrations of acetaldehyde obtained to estimate the expected contribution of total acetaldehyde point source by CALPUFF modeling showed the similar values to those measured in 'HAPs investigation in the region of Gumi-Daegu' and were consistent to the trend of civil odor complaints. Therefore, the expected contribution of total acetaldehyde point source was validated. The relative contribution of T company upon improving its emission facilities was predicted to be lowered by more than factor of two, compared to that prior to improving its emission facilities. To the contrary, the relative contribution of W company upon improving emission facilities of T company was predicted to be increased by more than factor of two, compared to that prior to improving emission facilities of T company. This indicates that the contribution of aldehyde point sources of W company was relatively increased upon improving emission facilities of T company.

• Journal of Korean Society for Atmospheric Environment
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• v.11 no.4
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• pp.339-349
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• 1995

The purpose on this study is to optimize the chromatographic determination method of trace aldehydes in ambient air. Carbonyl compounds in urban air were trapped at $C_{18}$ DNPH-coated cartridges, and generated hydrazone derivatives were separated by HPLC and detected by UV-vis spectroscopic detector at 360nm. Formaldehyde and acetaldehyde compounds could be isolated from urban(Seoul) air with more than 95% collection efficiency. The analytical detection limits for formaldehyde and acetaldehyde are 0.06pp $b_{v}$, 0.08pp $b_{v}$ for 108 L air samples, respectively. The precision of this method are 3 .sim. 4%(RSD) for mutiple injection of hydrazone standards. Separation of seven dinitrophenylhydrazones could be achieved in appoximately 20 minutes operation time using $C_{18}$ column with apprepriate eluent. The method was applied to the analysis of aldehydes and ketone in Seoul ambient air. The 24-h ambient levels of formaldehyde, acetaldehyde reached up to 6 .sim. 14 and 3 .sim. 8ppbv, respectively. The daily average concentration ratio were 0.60 for acetaldehyde/formaldehyde.yde.

• The Korean Journal of Food And Nutrition
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• v.30 no.4
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• pp.735-741
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• 2017

This study was conducted to investigate if the supplement formula may improve alcohol metabolism in healthy adult men. In a double-blinded, randomized, crossover study, subjects were administrated yeast extract mixtures (YEM, n=15), Hovenia dulcis mixtures (HDM, n=15), placebo (PLA, n=15), and control (CON, n=15) in an oral dose followed by one week washout periods. At each visit (0, 1, 2, 3, 4 week), subjects drank 450 mL, 20.1 percent alcohol after administered mixtures. Blood was drawn periodically (0, 0.25, 0.5, 1, 2, 4, 6, 15 hours). Fifteen subjects completed the protocol and were included in the analysis. Plasma ethanol concentration was lower in YEM (10 percent) and the HDM (5 percent) groups. The area under the curves (AUC) and $C_{max}$ for plasma ethanol were significantly decreased only in the YEM group, when compared with the CON group. The AUC and $C_{max}$ for plasma acetaldehyde concentration were significantly decreased in the YEM (45 and 54 percent) and the HDM (35 and 53 percent) groups respectively, when compared with PLA (p<0.01). Together, these findings validate that YEM or HDM improved alcohol metabolism and hangover syndromes, leading to reduce alcohol concentration and acetaldehyde concentration without adverse effects.

• Journal of Physiology & Pathology in Korean Medicine
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• v.21 no.2
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• pp.387-391
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• 2007

Recently, much attention has been paid to developing various kinds of fermented herbal extracts, a new type of traditional herbal medicine, in the field of Korean traditional medicine. The fermentation of medicinal herbs is intended to exert a favorable influence on digestability, bioavailavility and pharmacological activity of herbal extract in the gastrointestinal tract. It also produces a number of fermentation products that intensify the nutritional and pharmacological aspects of the medicinal herbs. In order to develop a functional beverage of alleviating the aftereffects of the previous drinks, the extracts (HP-1) of fermented herbal mixture, including Artemisia capillaris Thunb., Lonicera japonica Thunberg, and Hovenia dulcis Thunb., were prepared and the medicinal effect as a hangover cure was evaluated in ethanol-loaded rats. The enzyme activities of alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase-2 (ALDH2) were analyzed by measuring the concentration of blood ethanol and acetaldehyde. The mRNA expression of ADH and ALDH2 was also investigated through RT-PCR analysis. In the HP-1-treated group, the concentration of blood ethanol was significantly reduced at one hour after loading of ethanol, as compared to that in the saline-treated group. The reduced ethanol was converted to acetaldehyde, which resulted in rapid increase in acetaldehyde concentration in an hour. Acetaldehyde was started to decrease at 5 hours after ethanol loading. It implies that HP-1 is highly effective to stimulate the activities of ADH and ALDH2. The HP-1 treatment also activated the mRNA expression of ADH and ALDH. This study suggests that fermented herbal extract, HP-1 can be used as a functional beverage of alleviating the alcohol-induced hangover symptoms by stimulating the activities and gene expression of hepatic alcohol metabolizing enzymes.

• Korean Journal of Environmental Agriculture
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• v.30 no.3
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• pp.281-287
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• 2011

BACKGROUND: According to Korean regulations, bottled waters (BWs) can not be treated with chemical disinfectants like chlorine, so UV and ozone disinfection is applied. During the past several years, chemicals were detected in some BWs, and the public was concerned about the safety of BWs. METHODS AND RESULTS: Mineral waters were stored for 180 days at $25^{\circ}C$ and $50^{\circ}C$, tested acetaldehyde and formaldehyde by HPLC. When mineral waters were put in a PET bottles, the formaldehyde level ranged from 5 to $66{\mu}g/L$ during 180 days at $50^{\circ}C$. While the acetaldehyde level ranged from 31 to $221{\mu}g/L$, it was low than $16{\mu}g/L$ in glass bottle. CONCLUSION(s): This result showed that formaldehyde and acetaldehyde were detected higher in PET bottles than glass bottles, these also increased depending on the time of storage. Concentration of formaldehyde and acetaldehyde could be significantly influenced by the time of storage and temperature.