• Title/Summary/Keyword: absorption line

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Taurine Transporter Activity in the Human Colon Carcinoma cell Line(HT-29) is Decreased during Cell Differentiation (인체 소장상피세포주(HT-29)의 분화단계에 따른 타우린수송체 활성의 변화)

  • 박태선
    • Journal of Nutrition and Health
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    • v.33 no.6
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    • pp.660-667
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    • 2000
  • Previous studies on the effect of age on intestinal taurine transport in animals have invariably shown a decline in the activity of the transport system with increasing age. In the present study changes in taurine transporter activity were observed during cell differentiation in the human colon carcinoma cell line HT-29 This cell line exhibits various enterocytic characteristics when differentiated and therefore has frequently been used to study the characteristcs and regulation of nutrient and drug absorption in the small intestine at the cellular level. Pre-treatment of the cells with $\beta$-alanine(10mM) reduced the taurine transport activity to 33% of the value for the control cells(p<0.05) which implies that taurine and $\beta$-alanine share a common $\beta$-amino acid transport system for their celluar uptake in the HI-29 was continued until 21 days post seeding. Kinetic studies of the taurine transporter were conducted in the HT-29 cell line with varying taurine concentration(5-60$\mu$M) in the uptake medium Both Vmax and the Michaelis-Menten constant(Km) of taurine transporter were decreased as differentiation of the HT-29 cell line was progressed ; Vmax of the taurine transporter in cells incubated for 4, 14 and 21 days post seeding was 2.79$\pm$3.4m 16.89$\pm$1.74, and 0.85$\pm$0.08 and 0.32$\pm$0.01nmol.mg protein-1 .30min-1 respectively(p<0.001) and Km was 42.3$\pm$3.4, 16.89$\pm$1.74, and 11.2$\pm$3.0$\mu$M respectively (p<0.01) These results indicate that the activity of sodium dependent active taurine transport system in the HT-29 cell line is decreased as confluent cells are differentiated. This phenomenon in cell culture system corresponds well with the earlier observation of lower intestinal taurine transport activity in suckling rats compared to that in adult animals although direct relationship of cell differentiation with in vivo aging process needs further verification.

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A New k-Distribution Scheme for Clear-Sky Radiative Transfer Calculations in Earth's Atmosphere. Part II: Solar (Shortwave) Heating due to H2O and CO2

  • Ming-Dah Chou;Jack Chung-Chieh Yu;Wei-Liang Lee;Chein-Jung Shiu;Kyu-Tae Lee;Il-Sung Zo;Joon-Bum Jee;Bu-Yo Kim
    • Korean Journal of the Atmospheric Sciences
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    • v.78 no.9
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    • pp.2657-2675
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    • 2021
  • A new k-distribution scheme of longwave radiation without the correlated-k-distribution assumption is developed. Grouping of spectral points is based on the line-by-line (LBL)-calculated absorption coefficient k at a few sets of reference pressure pr and temperature θr, where the cooling rate is substantial in a spectral band. In this new scheme, the range of k(pr, θr) of a band is divided into a number of equal intervals, or g groups, in log10(kr). A spectral point at the wavenumber ν is identified with one of the g groups according to its kν(pr, θr). For each g group, a Planck-weighted k-distribution function Hg and a nonlinearly averaged absorption coefficient ${\bar{k}}_g(p,{\theta})$ are derived. The function Hg and the absorption coefficient ${\bar{k}}_g(p,{\theta})$ constitute the new k-distribution scheme. In this k-distribution scheme, a spectral point can only be identified with a g group regardless of pressure and temperature, which is different from the correlated-k distribution scheme. The k-distribution scheme is applied to the H2O, CO2, O3, N2O, and CH4 absorption bands, and results are compared with LBL calculations. To balance between the accuracy and the computational economy, the number of g groups in a band of a given gas is chosen such that 1) the difference in cooling rate is <0.1 K day-1 in the troposphere and <1.0 K day-1 in the stratosphere and 2) the difference in fluxes is <0.5 W m-2 at both the top of the atmosphere and the surface. These differences are attained with 130 g groups, which is the sum of the g groups of all five gases.

Saturation Absorption Spectroscopy for Two Photon Coherence of 85Rb D1 lines (85Rb D1선에서 이광자 결맞음을 고려한 포화흡수 분광)

  • Nho, J.W.;Kang, M.I.;Ryu, J.W.;Moon, H.S.
    • Korean Journal of Optics and Photonics
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    • v.17 no.4
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    • pp.305-311
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    • 2006
  • We propose a 7-level atom model, which takes into account two-photon coherence effects in saturation absorption spectroscopy. Using this model we explained spectral change with laser intensity and some of crossover resonance lines, which cannot be explained with Nakayama theory. The 7-level model consists of two upper levels and five lower levels, which account for $\pi-\pi$ polarization of both pump and probe beams in Zeeman sub levels. We compared our 7-level model with 4-level Nakayama theory for 5S$_{1/2}$ - 5P$_{1/2}$ transition line in $^{85}$Rb atoms. The results of the 7-level model calculation agree well the saturation absorption spectra data according to laser intensities.

Optimization of shock absorption system for lunar lander considering the effect of lunar regolith (달 토양 특성을 고려한 달착륙선 충격흡수장치의 최적화)

  • Yang, Soon Shin;Kang, Yeon Chul;Son, Jae Yeon;Oh, Min Hwan;Kim, Jeong Ho;Cho, Jin Yeon
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.42 no.4
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    • pp.284-290
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    • 2014
  • To successfully explore the moon by lunar lander, it is essential to guarantee the safe landing of lunar lander. Therefore, efficient shock absorption system of lunar lander should be designed in order to reduce landing impact force. Also, for more practical design of lunar lander, it is important to consider the effect of lunar regolith. In the line of thought, finite element model of lunar lander considering the effect of lunar regolith is developed. To reduce landing impact force, optimization of shock absorption system for lunar lander has been carried out. In optimization, sequential approximate optimization method based on meta-model is used. Through the result of optimization, it is verified that landing impact force on lunar lander can be efficiently reduced by the present optimization procedure.

STARS AS GRAVITATIONAL WAVE DETECTORS

  • KHOSROSHAHI H. G.;SOBOUTI Y.
    • Journal of The Korean Astronomical Society
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    • v.29 no.spc1
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    • pp.277-278
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    • 1996
  • In attempts to detect gravitational waves, the response of some celestial systems such as the earth[l] or binary systems[2] to such waves have been investigated. Following this line of thought, here we study the possibility of excitation of the oscillation modes of a polytropic star by gravitational radiation and calculate the relevant absorption cross sections.

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THE ASSOCIATION OF NEUTRAL HYDROGEN WITH HII REGIONS AND RADIO SOURCES

  • Minn, Young-Key
    • Journal of The Korean Astronomical Society
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    • v.18 no.2
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    • pp.100-110
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    • 1985
  • The HI features associated with HII regions and radio sources in the galactic-plane are searched in the Maryland-Green Bank Galactic 21-cm Line Survey. Among the twenty-eight such objects, twenty-five show HI depression features, two no feature, and an emission feature with excess HI brightness temperature. Most of these feature are surrounded by strong HI emissions. The depth of the HI depression is proportional to the radio continuum brightness temperature. The angular dimensions of the HI feature and radio source are comparable. The small HI depressions shown at the positions of HII region located in the outer solar circle are considered to be HI self-absorption features of very cold HI gas.

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Determination of Acetaminophen in Tablet Formulations by Second Derivative Ultraviolet Spectrometry (2차 미분 자외부 분광광도법에 의한 정제중의 아세트아미노펜 정량)

  • 유경수;고익배
    • YAKHAK HOEJI
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    • v.30 no.2
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    • pp.100-103
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    • 1986
  • The second derivative absorption spectra of acetaminophen in commercial tablet forms were measured without prior separation from other pharmaceutical adjuvants. The second derivative spectra of acetaminophen were found to have no influence on the characteristic absorbance band of it. A plot of 25 sets of the ordinate (Z) values with various acetaminophen concentrations mixed in other additives gave a straight line (correlation coefficient, 0.9999) and the slope was 2.901${\times}10^{-2}Z$ value/($\mu\textrm{g}$/ml). The procedures were sufficiently sensitive, precise and economical for the assay of tablets of acetaminophen.

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Luminescence, Excitation and Far-infrared Spectroscopy of cis-$\alpha$-Dichlorotriethyleneteraminechromium(III) Chloride

  • 최종하
    • Bulletin of the Korean Chemical Society
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    • v.19 no.5
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    • pp.575-579
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    • 1998
  • The 77 K luminescence and excitation spectra, and 298 K infrared and absorption spectra of $cis-{\Alpha}-[Cr(trien)Cl_2]Cl{\cdot}H_2O$ (trien=triethylenetetramine) have been measured. Ligand field electronic transitions due to spin-allowed and spin-forbidden are assigned. The zero-phonon line in the excitation spectrum splits into two components by $198\; cm^{-1}$, and the large $^2E_g$ splitting can be reproduced by the modern ligand field theory. It is confirmed that nitrogen atoms of the trien ligand have a strong σ-donor character, but chloride ligand has weak σ- and π -donor properties toward chromium(III) ion.