• 한국임상수의학회지
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• 제19권2호
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• pp.153-158
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• 2002

Ovaries from total 192 slaughtered cows(154 Korean native cows and 38 Holstein cows) were collected during the slaughtering process in Kimhae, Changyoung and Yangsan abattoirs in Kyungnam province from January 2001 to January 2002. In order to investigate incidence of the ovarian cysts, anatomical, histological observations were performed and also TUNEL methods and PCNA antibody by immunogistochemical methods for diagnostic accuracy of cysts in a few ovaries were applied. Apoptotic positive cells by TUNEL method appeared not or a few in cystic walls but appeared more number in normal large follicular walls and the proliferative positive cells by PCNA antibody appeared numerous in normal large follicular walls but not or a few in cystic walls. The incident rates of ovarian cysts were 19.5% in Korean native cows and 18.4% in Holstein cows. The incident rates of ovarian cysts in Holstein cows were lower than that of Koran native cows. The incident rates of follicular cysts and luteal cysts in Korean native cows were 11.7% and 7.8% respectively. The incident rates of follicular cysts and luteal cysts in Holstein cows were 10.5% and 7.9%, respectively. Higher incidence proportions of ovarian cysts according to seasons in Korean native cows were ordered as spring (29.8%), autumn (21.4%) winter (14.3%) and summer (6.7%). Rates of cows with single cyst and multiple cysts were 63.3%(19 heads /30 heads) and 36.7%(11 heads/30 heads) in 30 cystic Korean native cows, respectively. Cystic cows with corpus luteums were 50.0%(15 heads) in 30 Korean native cows and 42.9%(3 heads) in 7 dairy cows, respectively. Among 15 cystic Korean native cows with corpus luteums, rates of cows with single corpus luteum were 66.7%(10 heads) and rates of multiple corpus luteum were 33.3%(5 heads ), respectively. The average diameter of cysts and corpus luteums in cystic ovaries were 21.0$\times$17.1 mm and 18.1$\times$13.8 mm in 30 Korean native cows and 20.6$\times$17.7 mm and 19.3 $\times$ 14.9 mm in 7 Holstein cows, respectively. So the average sizes of cysts in cystic ovaries were larger than those of corpus luteums.

• 한국임상수의학회지
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• 제19권2호
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• pp.147-152
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• 2002

Ovaries from total 192 slaughtered cows, 154 Korean native cows and 38 dairy cows were collected during the slaughtering process in Kimhae, Changyoung and Yangsan abattoirs in Kyungnam province from January 2001 to January 2002. Rates of pregnant and non-pregnant and ovarian findings were invested. Rates of pregnant cows in 192 slaughtered cows were 12.5% (24 cows) and in difference of cow breeds, 11.0% (17 cows) in 154 Korean native cows and 18.4% (7 cows) in 38 dairy cows from total 192 cows, respectively. Ages of fetuses in pregnant Korean native cows were mostly less than 4 months and ages of fetuses in dairy cows were mostly about 7-8 months. Cows which each diameter of follicles and corpus luteums in same cow was more than 5-6 mm in diameter were 69.8% (134 cows) in total 192 slaughtered cows and in difference of cow breeds, 64.7% (11 cows) in 17 Korean native cows and 57.1% (4 cows) in 7 dairy cows. Mean diameter of foliicles and corpus luteums in Korean native cows are 13.7$\pm$5.6$\times$ 11.2$\pm$4.6mm and 17.5$\pm$4.6$\times$14.6$\pm$4.0 mm in non-pregnat cows, and are 11.0$\pm$4.8$\times$9.1 $\pm$ 2.6mm and 21.2$\pm$2.9$\times$18.3$\pm$ 2.7 mm in pregnant cows, respectively. Mean diameter of follicles and corpus luteums in dairy cows are 15.8$\pm$7.1 $\times$ 14.3$\pm$ 6.0 mm and 20.3$\pm$5.9$\times$16.9$\pm$ 5.8 mm in non-pregnant cows, and are 10.1 $\pm$ 3.0$\times$9.2$\pm$2.3 mm and 23.0$\pm$ 1.7$\times$20.1 $\pm$ 1.3 mm in pregnant cows, respectivley. The above findings indicate that the co-appearance rate of follicles and corpus luteums in same cows are higher in both pregnant and non-pregnant cows. Compared in pregnant and non-pregnant cow ovaries, mean size of follicles are smaller in pregnant cows but size of corpus luteums are more larger in pregnant cows than in non-pregnant cows. Correlation of the follicle size (Y) and corpus luteum size (X) in same cows developed each other in inversive size. Those correlative formulas appeared to be Y = -0.2022X+17.175 in Korean native cows and Y= -0.5754 X+24.153 in dairy cows.

• 한국수정란이식학회지
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• 제27권1호
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• pp.57-62
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• 2012

Efficient oocyte activation is a key step for the success of nuclear transfer in cloning. Ionomycin sequentially combined with 6-DMAP is now widely used to activate normal oocytes for analytical studies of oocyte activation and to activate reconstructed oocytes after nuclear transfer. The present study investigated sources of oocytes, duration of ionomycin and 6-DMAP, laser and electric stimulation in goat oocyte activation in order to optimize the protocols. Goat ovaries were collected in individual abattoirs during the breeding season and were delivered to the laboratory within 6 h in saline with 100 IU/ml streptomycin and 0.05 mg/ml penicillin. The oocytes were denuded from the cumulus cell by pipetting with 0.2% hyaluronidase in PBS at 20~22 hr post maturation. Oocytes with the polar body were selected and assigned to four groups for parthenogenetic activation. To examine the effect of duration of ionomycin treatment, oocytes after 20~22 hr of maturation were treated with 2.5 uM ionomycin for 1 or 5 min times and then cultured in 2 mM 6-DMAP for 2 or 4 hr. The activated oocytes were cultured in mSOF at $38.5^{\circ}C$ in $CO_2$ 5%, $O_2$ 5% and $N_2$ 90% multi incubator. Cleavage and blastocyst development was observed at 48 hr and day 8 of culture $in$ $vitro$, respectively. Activation rates of oocytes exposed to ionomycin for 1 min(86.4%) were significantly higher than those treated for 5 min(74.3%) duration. This indicated that 1 min ionomycin treatment was most suitable for activation of goat oocytes. The duration of 6-DMAP treat duration was in 2 mM 6-DMAP for 2 hr after 1 min exposure to 2.5 uM ionomycin. The activation rate of oocytes incubated in 6-DMAP for 2 hour(82.5%) was significantly higher than those in oocytes treated with 4 hr(75.5%).

• 한국동물위생학회지
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• 제34권4호
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• pp.321-331
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• 2011

Mycobacterium bovis, a member of the M. tuberculosis complex (MTC), is the causative agent of bovine tuberculosis. Detection of M. bovis and M. tuberculosis using conventional culture- and biochemical-based assays is time-consuming. Therefore, a simple and sensitive molecular assay for rapid detection would be of great help in specific situations such as faster diagnosis of bovine tuberculosis (bTB) infection in the abattoirs. We developed a novel multiplex real-time PCR assay which was applied directly to biological samples with evidence of bTB and it was allowed to differentiate between M. bovis and M. tuberculosis. The primers and TaqMan probes were designed to target the IS1081 gene, the multi-copy insertion element in the MTC and the 12.7-kb fragment which presents in M. tuberculosis, not in the M. bovis genome. The assay was optimized and validated by testing 10 species of mycobacteria including M. bovis and M. tuberculosis, and 10 other bacterial species such as Escherichia coli, and cattle lymph nodes (n=113). The tests identified 96.4% (27/28) as M. bovis from the MTC-positive bTB samples using conventional PCR for specific insertion elements IS1081. And MTC-negative bTB samples (n=85) were tested using conventional PCR and the real-time PCR. When comparative analyses were conducted on all bovine samples, using conventional PCR as the gold standard, the relative accuracy of real-time PCR was 99.1%, the relative specificity was 100%, and the agreement quotient (kappa) was 0.976. The detection limits of the real-time PCR assays for M. bovis and M. tuberculosis genomic DNA were 10 fg and 0.1 pg per PCR reaction, respectively. Consequently, this multiplex real-time PCR assay is a useful diagnotic tool for the identification of MTC and differentiation of M. bovis and M. tuberculosis, as well as the epidemiologic surveillance of animals slaughtered in abattoir.

• 대한수의학회지
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• 제35권2호
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• pp.327-336
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• 1995

This study was carried out to develop the animal protein(feed for fry) that was isolated, purified and lyophilized the rumen ciliates from the healthy rumen contents which have $10^5-10^6/g$ ciliates and were discarded in abattoirs. The rumen ciliates are non-pathogenic, anaerobic and the weight of this protozoa is 2% of rumen content. The rumen protozoan and bacterial proteins both have a biological value for rats of 80-81, which is higher than the 72 of brewer's yeasts. Furthermore, the true digestibility and net protein utility of the protozoan protein are 91 and 73, much higher than those of bacterial(74 and 60) or yeast(84 and 60) proteins. The amino acids of rumen protozoa is nutritionally superior than the others. The size of rumen ciliates is $30-200{\times}20-110{\mu}m$ and so we had isolated and purified the rumen ciliates from the rumen contents by the physical methods. The purified rumen protozoa was lyophilized with freezing dryer. The results of this experiment were as follows : 1. Population dynamics of protozoan ciliates in slaughtered rumens; % of samples which small ciliates were predominated was 82.5%(52/63) and that of large ciliates was 17.5%(11/63). 1) predominant species of small ciliates were Entodinium ovinum and E nanellum. 2) predominant species of large ciliates were Epidinium ecaudatum and Diploplastron affine. 2. The lyophilized rumen ciliates which were isolated and purified from 1 kg of rumen content at the pH 6.2-6.8 was about 7.0 gram. 3. The nutrient analysis of lyophilized rqmen ciliates(LRC) was as follows: 1) Proximate analysis of the LRC and the composition of fry feed; moisture 8.05%(below 10.0), protein 35.37%(45), fat 5.39%(4.5), fiber 1.23%(below 2.5), ash 2.25%(below 15.0), Ca 0.26%(below 2.0), P 0.14%(below 1.1), energy 4,608.11(fish meal 5000 cal/g) 2) Amino acids (% in crude protein) of the LRC and the rotifer(Brachionus plicatilis); Arg 5.19%(4.50), His 2.50%(1.55), Ile 5.29%(3.45), Leu 8.11%(5.85), Lys 10.34%(6.15), Met 2.25% (0.85), Phe 5.66%(3.80), Thr 5.14% (3.45), Val 4.18%(3.90), Ala 4.13%(3.35), Asp 13.26%(8.25), Glu 16.62%(9.20), Gly 4.23%(3.10), Pro 3.25%(5.05), Ser 4.85%(3.85), Tyr 5.04%(3.05) 3) Fatty acids(% in fat) of the LRC and the rotifer(biological feed ; Brachionus plicatilis); myristic acid(C14:0) 3.27%(0.3), myristoleic acid(C14:1) 0.83%(-), palmitic acid(C16:0) 39.11% (23.5), palmitoleic acid(C16:1) 2.81%(2.0), stearic acid(C18:0) 9.36%(5.6), oleic acid(C18:1) 25.54%(3.5), linoleic acid(C18:2) 15.05%(32.9), linolenic acid(C18:3) 1.74%(9.8). Judging from the above investigated results, the analytical data of proximate analysis, amino acids, fatty acids of the purified and lyophilized rumen protozoa are reasonable for the feed of freshwater fishes(fry and fingerling). But it was disappointed of our expectation that the crude protein of lyophilized rumen ciliates contains low percentage, it was thought that because of the small ciliates(starch digester) in beef cattle rumens which were administered the concentrated feed, is much difficult to isolate and purify than the large ciliates(fiber digester).