• Korean Journal of Fisheries and Aquatic Sciences
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• v.8 no.4
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• pp.234-241
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• 1975

Reproductive cycle of Haliotis discus hannai Ino was studied based on the samples which were collected in Yeosu area from January, 1973 to December, 1974. Particular emphasis was paid on the seasonal variation of gonad maturity factor, coefficieot of gonad maturity, fatnesss, and relationship of gonad weight to growth of the abalone. seasonal fluctuation of water temperature and induction of artificial spawnings were also checked. The abalone of this area spawns twice a year: spring spawning(early May-late July) and Autumn spawning(early September-late November). peak spawning occurs from late May to middle of June for spring spawning: it occurs from late September to early November for Autumn spawning. Gonad maturity factor was closely related to the spawning seasons. For the spring spawning Gonad maturity factor reached to its maximum value as 18.84 in April; for the Autumn spawning it reached to its maximum value as 22.65 in September. Annual minimum value was 12.65 in January. Annual maximum values of coefficient of gonad maturity were 44.10 in May and 49.05 in September and the annual minimum was 10.09 in January. Fatnesswas highest in July as 0.221 and it was lowest in October as 0.1058. Ratios of shell length to gonad weight were formulated as follows : $$In\;September\;GW=0.000003710SL^{3.7860}$$ $$In\;January\;GW=0.000021198SL^{2.8564}$$ $$Annual\;mean\;GW=0.00000005013SL^{4.2273}$$ Relations of total weight to gonad weight were formulated as follows : In September GW=0.1918TW-4.9231 In January GW=0.0992TW-0.1759 Annual maan GW=0.1568UW-3.5256.

• The Korean Journal of Malacology
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• v.29 no.3
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• pp.181-187
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• 2013

In order to investigate environmental stress inducible genes in abalone, we analyzed differentially expressed transcripts from a Pacific abalone, Haliotis discus hannai, after exposure to heat-, cold- or hyposalinity-shock by suppression subtractive hybridization (SSH) method. 1,074 unique sequences from SSH libraries were composed to 115 clusters and 986 singletons, the overall redundancy of the library was 16.3%. From the BLAST search, of the 1,316 ESTs, 998 ESTs (75.8%) were identified as known genes, but 318 clones (24.2%) did not match to any previously described genes. From the comparison results of ESTs pattern of three SSH cDNA libraries, the most abundant EST was different in each SSH library: small heat shock protein p26 (sHSP26) in heat-shock, trypsinogen 2 in cold-shock, and actin in hyposalinity SSH cDNA library. Based on sequence similarities, several response-to-stress genes such as heat shock proteins (HSPs) were identified commonly from the abalone SSH libraries. HSP70 gene was induced by environmental stress regardless of temperature-shock or salinity-stress, while the increase of sHSP26 mRNA expression was not detected in cold-shock but in heat-shock condition. These results suggest that the suppression subtractive hybridization method is an efficient way to isolate differentially expressed gene from the invertebrate environmental stress-response transcriptome.

• Journal of Microbiology and Biotechnology
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• v.34 no.2
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• pp.379-386
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• 2024

Basophils and mast cells are specialized effector cells in allergic reactions. Haliotis discus hannai (abalone), is valuable seafood. Abalone male viscera, which has a brownish color and has not been previously reported to show anti-allergic activities, was extracted with acetone. Six different acetone/hexane fractions (0, 10, 20, 30, 40, and 100%) were obtained using a silica column via β-hexosaminidase release inhibitory activity-guided selection in phorbol myristate acetate and a calcium ionophore, A23187 (PMACI)-induced human basophils, KU812F cells. The 40% acetone/hexane fraction (A40) exhibited the strongest inhibition of PMACI-induced-β-hexosaminidase release. This fraction dose-dependently inhibited reactive oxygen species (ROS) production and calcium mobilization without cytotoxicity. Western blot analysis revealed that A40 down-regulated PMACI-induced MAPK (ERK 1/2, p-38, and JNK) phosphorylation, and the NF-κB translocation from the cytosol to membrane. Moreover, A40 inhibited PMACI-induced interleukin (IL)-1β, IL-6, and IL-8 production. Anti-allergic activities of A40 were confirmed based on inhibitory effects on IL-4 and tumor necrosis factor alpha (TNF-α) production in compound (com) 48/80-induced rat basophilic leukemia (RBL)-2H3 cells. A40 inhibited β-hexosaminidase release and cytokine production such as IL-4 and TNF-α produced by com 48/80-stimulated RBL-2H3 cells. Furthermore, it's fraction attenuated the IgE/DNP-induced passive cutaneous anaphylaxis (PCA) reaction in the ears of BALB/c mice. Our results suggest that abalone contains the active fraction, A40 is a potent therapeutic and functional material to treat allergic diseases.

• The Korean Journal of Malacology
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• v.31 no.2
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• pp.83-92
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• 2015

The effect of different intermediated rearing method by expanding the attached floor space in order to highly density culture on the growth characteristics and survival rate of the juvenile abalone, Haliotis discus hannai, were investigated in land-based tanks. The intermediated culture methods was determined thru the shelter counts and layer for 10 month with two replicates : the single layer shelter (SLS), the double layer shelter (DLS), the triple layer shelter (TLS) and the single layer shelter under net cage (SLSNC). In addition, the culture on shallow race way tank had to set up as culture of the ditch raceway tank (CDRT) and the floor race way tank (CFRT). In the growth performance of reared abalone (initial mean shell length $54.18{\pm}7.39mm$ and weight $1.93{\pm}0.14g$) at experimental tanks, that the absolute growth rate (ARG), daily growth rate (DGR) and specific growth rate (SGR) to the shell length and shell breadth was not significant at each experimental tanks except SLSNC. As well as too, weight gain (WG), daily weight gain (DWG) and specific weight gain (SWG) to weight was not significant at each experimental tanks except SLSNC, too. Survival rates of CDRT and CFRT was lower than those of different experimental tanks (P < 0.05). Therefore, these results is showed that high density different intermediated rearing method by expanding the attached floor space for juvenile H. discus hannai was not have difference as growth performance and survival rate both one layer shelter and multi layer shelter. Also, it is considered that shallow race way tank was not useful rearing for the juvenile intermediate culture of H. discus hannai in land based.

• Food Science and Preservation
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• v.17 no.4
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• pp.533-540
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• 2010

Overall composition, and fatty acid, amino acid, and nucleotide-type compound levels in wild (Haliotis gigantea) and cultured abalone (Haliotis discus hannai), were investigated. Wild abalone had a higher moisture content than did cultured abalone, but the converse was true for crude protein content. In overall composition, crude lipid level was higher in the viscera than in the meat, with the greatest level, $2.02{\pm}0.15%$ (w/w), observed in the viscera of wild abalone. The major fatty acids were palmitic acid (16:0), oleic acid (18:1n-9), eicosatrienoic acid (20:3n-3, ETA), eicosapentaenoic acid (20:5n-3, EPA), and docosahexaenoic acid (22:6n-3, DHA). The omega-3 fatty acid content (EPA and DHA) was higher in wild than in cultured abalone. A total of 17 amino acids were detected in all abalone samples, most of which had high levels of aspartic acid, glutamic acid, glycine, and arginine, and low amounts of cysteine, methionine, and histidine. Glutamic acid was the most abundant of all amino acids. The content of free amino acids was related to taste score. The major free amino acids were taurine, alanine, and arginine, of which taurine was the most abundant, and was present at higher levels in wild compared to cultured abalone. The total contents of nucleotide-related compounds in wild and cultured abalone were 12.93 mg/100g and 30.75 mg/100g, respectively.

• The Korean Journal of Malacology
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• v.29 no.4
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• pp.303-311
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• 2013

This study investigated survival, falling and structural changes of foot in Haliotis discus hannai with various water temperature and salinity. Experimental conditions were composed of control with $20^{\circ}C/33.5$ psu, higher temperature group with 23, 26, 29 and $32^{\circ}C$, lower temperature group with 3, 6, 9 and $12^{\circ}C$, and different salinities group with 33.5, 26.8, 20.1, 13.4, 6.7 and 3.3 psu. Experimental period was 7 days. Survival and falling rate of the abalone exposed to 7 days at control and experimental condition of $12^{\circ}C$ and $23^{\circ}C/33.5$ psu were 100% and 0%, respectively. Mortality and falling rate of the abalone exposed to the different temperature and salinity were dose dependent by experimental condition. Histopathological changes in epithelial and muscular layer of foot showed more obvious with experimental condition of salinity decrease than different temperature condition.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.74-74
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• 2003

In present study, attempts were made to preserve abalone (Haliotis discus hannai) sperm in liquid form at low temperature, to evaluate the effect of various diluents in short-term storage on sperm, and cryopreservation procedures were optimized for the cryoprotectants as well as freezing rates, in terms of the motility and survival rate, and the ultrastructural changes of sperm after short-term storage and cryopreservation were observed. The abalone sperm reached maximum motility until about 4min after activation. The motility was constant for about 16min, after which it dropped gradually, and about 50min later all motility ceased. Threshold activation of sperm was found in 40% artificial seawater (ASW), and motility increased as the concentration of ASW increased. In Hanks balanced salt solution without calcium (Ca-Free HBSS, 300 and 400 mOsmol/kg) and 10%, 20%, and 30% ASW the sperm was immotile, and motility once again restored incompletely only in HBSS of 300 and 400 mOsmol/kg, 20% and 30% ASW after 100% ASW was added. Sperm motility was extended following 20 days of cold storage only in 70% and 100% ASW. A high motility index of 3.5-4.5 was observed for the first 8 days in 70% and 80% ASW. In other diluents sperm motility was constant less than 10 days, and the motility index was obviously lower than that of sperm in 70% and 100% ASW. After 20 days of cold storage survival rates of 10.2%-20.7% were obtained in ASW and 300 mOsmol/kg HBSS, and that in 400 HBSS (65.3%) was significantly higher than others. The constant period of sperm motility stored in 70% ASW was longer obviously than that in 100% ASW after 6 days of storage, and the time to maximum motility of sperm stored in 70% increased gradually, while the difference in which of sperm in 100% ASW was not significant. The sperm plunged into liquid nitrogen all died except that sperm using 15% glycerol as cryoprotectant restored 10.4% of motility. The highest motility index (3.4) was obtained with 5% glycerol and freezing procedure: $50^{\circ}C$/min from $20^{\circ}C$ to $-80^{\circ}C$.

• The Korean Journal of Malacology
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• v.29 no.3
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• pp.233-238
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• 2013

The effect of three different diets (formulated diet, FD; Undaria pinnatifida, UP; Laminaria japonica, LJ) on growth in the Haliotis discus hannai for 90 days was investigated. The shell length of UP ($80.62{\pm}1.92$ mm) and LJ ($81.14{\pm}1.16$ mm) were significantly faster than those of FD ($79.38{\pm}1.69$ mm) (P < 0.05). However, shell breadth and total weight were no significant difference among three diets. On the other hand, the weight gain of FD (16.65% for 0-45day and 25.71% for 45-90day) tend to have higher than those of UP (14.57% and 23.30%) and LJ (12.65% and 24.51%). This results shows that seaweed diets (UP and LJ) help the shell growth of abalone and formulated diet (FD) help the weight gain of abalone. Therefore, the growth of shell and muscle will depend on different diets.

• Proceedings of the Korean Aquaculture Society Conference
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• 2003.10a
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• pp.44-44
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• 2003

Gene expression in five tissues of the abalone (Haliotis discus hannai) was investigated using an expressed sequence tag (EST) analysis. Randomly selected clones were obtained from cDNA libraries constructed with gill (GI), digestive diverticula(DD), hepatopancreas (HP), foot/mucus (FM) and rectangular muscle (RM). Of 1,235 clonesanalyzed (288 clones for GI, DD, HP each,166 for FM, and 205 for RM), 741 (60.0%) clones in total turned out to share significant similarity with the sequences from NCBI GenBank (less than 10/sup -3/ of e-values), 423 sequences showed poor similarity (> 10/sup -3/), and 71 sequences didn't match with any sequences in GenBank. The percent unique sequence (singleton) was ranged from 56.1% (RM) to 74.7% (FM) among libraries. On the other hand, overall percent singleton was 55.3% when all the ESTs from five libraries were assembled into contigs. Analysis of the organisms represented by the best hit for each EST (e-values < 10/sup -3/) showed that 23.8% matched with mammalian entries, 24.0% with mollusks, 14.4% with insects, 11.6% with fish and 26.2% with others. The expressed patterns differed among the tissues when judged by the categorization of the sequences from each library into 10 broad functional classes. In all the libraries, the class I (no hit o. poor similarity) was the largest category with an average of 40.1%. This largest class was followed by class V (general metabolisms) in DD (21.9%), GI (14.6%) and HP (16.7%), while the 'cell structure and motility'(class VI) was the second largest class in remaining two libraries (31.2% for RM and 9.6% for FM). The class IX (cell division and proliferation) was the smallest class in all the libraries (less than 3%). This report provides the first tissue-specific lists of expressed abalone genes, which could be a fundamental basis for genomics program of abalone species.

• The Korean Journal of Malacology
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• v.32 no.1
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• pp.1-7
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• 2016

The developmental stage of germ cells during oogenesis can be categorized into six stages with histological features: (1) oogonium, (2) previtellogenic oocyte, (3) initial vitellogenic oocyte, (4) early active vitellogenic oocyte, (5) late active vitellogenic oocyte and (6) ripe oocyte. The size of oocyte, nucleus and nucleolus illustrated the increase tendency but size ratio of nucleolus to nucleus was decreased during oogenesis. During oogenesis the stainability in the cytoplasm of oocyte changes from basophilic to eosinophilic in H-E stain. And egg stalk and outer jelly membrane was developed in the oocyte. These histological changes are seemed to be yolk accumulation in the oocyte and preparation process for spawning.