• Applied Biological Chemistry
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• v.24 no.4
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• pp.238-244
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• 1981

The thin Layer chromatographic determination of ${\alpha}-solanine $ in potato tuber was investigated. The solanine was extracted with methanol-chloroform mixture (2 : 1, v/v) and precipitated as the crude glycoalkaloid by the addition of ammonium hydroxide. The solanine in the crude precipitate was separated by TLC using a solvent system of 1% $NH_4OH-absolute$ ethanol-chloroform (1:2:2, v/v). The purity of ${\alpha}-solanine $ was confirmed by infrared spectrophotometry. The ${\alpha}-solanine $ separated by TLC was determined at the wavelength of 310nm after coloration with concentrated sulfuric acid-1% paraformaldehyde, with the molar absorptivity of 2,090.

• Korean Journal of Food Science and Technology
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• v.16 no.4
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• pp.388-391
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• 1984

The purpose of this report was to investigate change in glycoalkaloid content (${\alpha}-chaconine$, ${\alpha}-solanine$) which contained in the cortex and medulla part during storage of two varieties of potato, Irish Cobbler and May Queen at $1^{\circ}C$ and $20^{\circ}C$. The tubers of both varities stored at $20^{\circ}C$ showed sprouting and ${\alpha}-chaconine$ and ${\alpha}-solanine$ contained in cortex part increased, particularly the extent of increase was more noticeable in May Queen than in Irish Cobbler. The content of glycoalkaloid in tubers stored at $1^{\circ}C$ also increased continuously. A small amount of ${\alpha}-chaconine$ was detected in the medulla part of both varieties, but ${\alpha}-solanine$ was not detected at all.

• Korean journal of food and cookery science
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• v.23 no.2 s.98
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• pp.245-251
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• 2007

The purpose of this study was to examine how potato glycoalkaloid(PGA) contents changed as storing temperatures and periods changed for potatoes(Superior) cultivated in Korea. Some potatoes were stored at $5^{\circ}$C for 80 days, and others were stored at $20^{\circ}$C for 40 days. PGA contents were measured every 20 days for the potatoes stored at $5^{\circ}$C and 10 days for those stored at $20^{\circ}$C. The results can be summarized as follows. The amounts of a-chaconine and a-solanine measured on the first day of storage were 54.22 mg/100 g and 26.57 mg/100 g, respectively. Thus, the a-chaconine content was almost twice as much as the a-solanine content. The sprouts of potatoes stored at $5^{\circ}$C grew by 0.4 cm in 20 days, 1 cm in 40 days, 1.8 cm in 60 days, and 5.2 cm in 80 days. Furthermore, the PGA content increased by 3.5% in 20 days, 11.6% in 40 days, 23.4% in 60 days, and 41.4% in 80 days, compared to the PGA content on the first day. The sprouts of potatoes stored at 20 grew by 0.5 cm in 10 days, 2.3 cm in 20 days, and 7.4 cm in 40 days. Furthermore, PGA content increased by 12.5% in 10 days, 36.6% in 20 days, 44.8% in 30 days and 48.4% in 40 days, compared to the PGA content on the first day. Thus, we determined that potatoes stored at $20^{\circ}$C showed faster sprout growths and faster increases in PGA content than those stored at $5^{\circ}$C.

• Proceedings of the Korean Society of Food and Cookery Science Conference
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• 2003.05a
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• pp.102-102
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• 2003

감자에는 적은 양이지만 자연독의 종류인 glycoalltaloid가 함유되어 있다. glycoalkaloid의 대표적인 성분은 a-solanine과 B-chaconine으로 이들을 20rng 이상 섭취할 때에는 건강에 유해하고 그 함량이 많을 시에는 쓴맛의 원인이 된다. 지금까지의 생감자에 함유된 glycoalkaloid에 관한 연구는 몇편 보고된 바 있으나, 실제 소비자가 구매하여 섭취하는 감자 가공제품에 관한 연구는 찾아보기드문 실정이다. 따라서 본 연구는 시판 감자 가공품을 시료로 하여 그에 함유되어 있는 glycoalkaloid의 함량을 조사히여 시판 감자 가공품에 대한 몇 가지의 정보를 제공하고자 한다. (중략)

• Food Science and Biotechnology
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• v.18 no.1
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• pp.113-117
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• 2009

A method was developed using enhanced liquid chromatography coupled with electrospray ionization mass spectrometry for the analysis and quantitation of 2 main potato glycoalkaloids, $\alpha$-chaconine, and $\alpha$-solanine, without any pre-concentration or derivatisation steps. Calibration curves generated by this technique exhibited a linear dynamic range from 0.025 to $50{\mu}g/mL$ and from 0.05 to $50{\mu}g/mL$ for $\alpha$-chaconine and $\alpha$-solanine, respectively. Matrix effects were evaluated by comparing calibration curves measured in matrix-matched and solvent-based systems. Ion suppression due to matrix effects was weak and extraction recoveries of 88 to 114% were obtained in different sample matrices spiked with analyte concentrations ranging from 15 to $35{\mu}g/mL$. Potatoes that had been genetically modified to tolerate glufosinate contained the same glycoalkaloid levels as their non-transgenic counterpart. We suggest complementing compositional comparison assessment strategy by validating quantitative analytical methods for the toxic glycoalkaloids in potato plants.

• Journal of the East Asian Society of Dietary Life
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• v.15 no.2
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• pp.207-212
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• 2005

To determine changes of chlorophyll and potato glycoalkaloid(PGA) contents in potato tubers after lightening, Dejima potatoes cultivated in Korea were used and PGA was analyzed by HPLC. Potatoes were stored under fluorescent and UV light exposure or darkness for 7 days at $5^{\circ}C$. The contents of chlorophyll and glycoalkaloid in the peel of potatoes exposed to fluorescent light were increased to $84\%,\;69.5\%$ respectively compared to potatoes stored without lightening. While a marked effect of lightening on the peel of potatoes exposed to fluorescent light, UV light lead to no change of chlorophyll and less increase in glycoalkaloid than fluorescent did. The increase of chlorophyll was provoked by only fluorescent light. Cortex part did not accumulate any chlorophyll or glycoalkaloid.

• Journal of the Korean Home Economics Association
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• v.42 no.4
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• pp.187-194
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• 2004

The concentration of potato(Solanum tuberosum L.) glycoalkaloids(PCA) (i.e., ${\alpha}$-chaconine and ${\alpha}$-solanine) in Korean cultivated potato plant, and in the different varieties, parts and weights of the potato tubers, were determined by high-performance liquid chromatography(HPLC). The highest concentrations of PGA in potato plants were found in the roots, followed by the stems and leaves. A large quantity of PGA existed in the periderm of 'Atlantic' potatoes, whereas 'Irish Cobbler' contained the lowest amount in five cultivated potato tubers.'Irish Cobbler' potatoes were divided into three parts and the PGA content in each part was determined. It was found that both of the end parts of the potatoes contained higher PGA than the middle part. The PGA contents in four different sizes of potatoes increased toward the smaller size. Thesignificantly high level of PGA was contained in the smallest size potato tubers, which have been used widely for Korean cooking.

• Journal of the East Asian Society of Dietary Life
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• v.16 no.5
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• pp.600-606
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• 2006

This survey was conducted to investigate the usage of potato and recognition of glycoalkaloid among residents aged from their teens to over fifties in Daegu city and Gyeongbuk Province. The preferred purchase places for the respondents were traditional markets (41.8%), big discount markets (23.8%), and supermarkets (14.9%), in order. Freshness (52.1%) was the most important criterion followed by size (12.5%), sprout (10.1%) and producing district (6.6%), in order. Most (77.6%) respondents preferred small amount below $2{\sim}3kg$ per purchase. Potato recognition revealed that respondents knew relatively well that potato sprouts contain toxins (M=4.30), that the major potato toxin is solanine (M=3.86) and that potato contains toxins when its color turns to green (M=3.70). However, respondents did not recognized well that the potato peel contains toxins (M=3.00), or that this toxin is chaconine (M=2.48).

• Korean journal of food and cookery science
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• v.20 no.1
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• pp.76-80
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• 2004

The potato tubers of Solanum tuberosum L. contain potato glycoalkaloids (PGA), a class of naturally occurring toxicants. The primary constituents of this class of compounds in potatoes are $\alpha$-chaconine and $\alpha$-solanine. The purpose of this study was to examine the effects of various cooking methods on the PGA contents in the common Korean potato Irish Cobbler variety and those in commercially produced potato products consumed in Korea. After cooking, most potatoes showed big decreases in their PGA contents compared to the uncooked samples, with the exception of baked potatoes, which maintained 90.86％ of their PGA contents. The PGA levels in boiled and blanched potatoes were reduced by 91.68 and 84.17％, respectively. However, the levels in samples boiled in 1％ salted water were only reduced by 52.10％. Potatoes fried and sauteed in oil had their PGA contents reduced to 49.42 and 49.51％ of their original levels. The reductions in the relative PGA contents by cooking method were in the order boiling〉blanching〉boiling with 1％ NaCl〉steaming〉sauteing〉frying〉microwaving〉baking. The highest remaining PGA content of the potato products in Korea was in potato chips, at 69.57mg/100g, but made with imported potatoes.

• Korean journal of applied entomology
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• v.39 no.3
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• pp.157-163
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• 2000

In a previous report, 50 potato cultivars were screened for infestation levels by the coppery click beetle (Selatosomus puncticollis Motschulsky) in the field. Subsequently, we selected 10 potato cultivars (Anco, Atlantic, Bintje, Dejima, Denali, Jopung, Irish Cobbler, Namsuh, Shepody, and Superior) to evaluate feeding preferences by wireworms, and to analyze some factors associated with resistance. The injury rates and number of holes in potato tubers damaged by larvae of S. puncticollis were checked in the field and laboratory. Additionally, some of their chemical characters (contents of glycoalkaloids, total-nitrogen, Ca, K, Mg, sugars, and starch) were quantified. And finally correlation analysis was conducted to see whether there is a possible relationship between these characteristics and the damage level. The tuber injury rates by S. puncticollis larvae were generally high showing 19% to 73% of damage level. The highest number of tuber hole damaged by S. puncticollis larvae was found on cv. Namsuh, but generally fewer on cvs. Anco, Atlantic, Bintje, Denali and Superior. No activities for $\alpha$-chaconine, $\alpha$-solanine and $\alpha$-tomatine at a concentration of 2,500 ppm were found to S. puncticollis larvae. The contents of glycoalkaloids in tuber were different depending on cultivars. In tubers, cv. Superior contained the highest level of 18.8 mg%, but cv. Irish Cobbler had the lowest level of 6.39 mg%. Concentrations of reducing sugars and total free sugars in tubers of cv. Namsuh were 0.71 % and 2.95%, but 0.26% and 1.77% in those of cv. Dejima, respectively. For the content of potato starch, cvs. Bintje, Dejima and Irish Cobbler showed higher level, but cvs. Jopung and Shepody lower. The highest contents of total nitrogen, Ca, K and Mg in tuber were found on cvs. Anco, Atlantic, Anco and Jopung, respectively. From correlation analyses, injury rate by S. puncticollis larvae was correlated with total nitrogen content (r = -0.71435*) and total sugar content in tuber (r = 0.78018*). Such information will become essential in developing integrated pest management programs and also in breeding new potato cultivars resistant to the wireworms.