• Journal of Microbiology and Biotechnology
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• 제28권6호
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• pp.1030-1036
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• 2018

Bacillus strains produce various types of antibiotics, and random mutagenesis has traditionally been used to overproduce these natural metabolites. However, this method leads to the accumulation of unwanted mutations in the genome. Here, we rationally designed a single nucleotide substitution in the degU gene to generate a B. subtilis strain displaying increased plipastatin production in a foreign DNA-free manner. The mutant strain (BS1028u) showed improved antifungal activity against Pythium ultimum. Notably, pps operon deletion in BS1028u resulted in complete loss of antifungal activity, suggesting that the antifungal activity strongly depends on the expression of the pps operon. Quantitative real-time PCR and lacZ assays showed that the point mutation resulted in 2-fold increased pps operon expression, which caused the increase in antifungal activity. Likewise, commercial Bacillus strains can be improved to display higher antifungal activity by rationally designed simple modifications of their genome, rendering them more efficient biocontrol agents.

• ETRI Journal
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• 제45권1호
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• pp.119-130
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• 2023

We propose a quantized gradient search algorithm that can achieve global optimization by monotonically reducing the quantization step with respect to time when quantization is composed of integer or fixed-point fractional values applied to an optimization algorithm. According to the white noise hypothesis states, a quantization step is sufficiently small and the quantization is well defined, the round-off error caused by quantization can be regarded as a random variable with identically independent distribution. Thus, we rewrite the searching equation based on a gradient descent as a stochastic differential equation and obtain the monotonically decreasing rate of the quantization step, enabling the global optimization by stochastic analysis for deriving an objective function. Consequently, when the search equation is quantized by a monotonically decreasing quantization step, which suitably reduces the round-off error, we can derive the searching algorithm evolving from an optimization algorithm. Numerical simulations indicate that due to the property of quantization-based global optimization, the proposed algorithm shows better optimization performance on a search space to each iteration than the conventional algorithm with a higher success rate and fewer iterations.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제11권1호
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• pp.302-320
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• 2017

In this paper, an innovative robust feature detection and matching strategy for visual odometry based on stereo image sequence is proposed. First, a sparse multiscale 2D local invariant feature detection and description algorithm AKAZE is adopted to extract the interest points. A robust feature matching strategy is introduced to match AKAZE descriptors. In order to remove the outliers which are mismatched features or on dynamic objects, an improved random sample consensus outlier rejection scheme is presented. Thus the proposed method can be applied to dynamic environment. Then, geometric constraints are incorporated into the motion estimation without time-consuming 3-dimensional scene reconstruction. Last, an iterated sigma point Kalman Filter is adopted to refine the motion results. The presented ego-motion scheme is applied to benchmark datasets and compared with state-of-the-art approaches with data captured on campus in a considerably cluttered environment, where the superiorities are proved.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제17권3호
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• pp.1035-1048
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• 2023

The IEEE 802.11 WLAN adopts a random backoff algorithm for its collision avoidance mechanism, and it is well known that the contention-based algorithm may suffer from performance degradation especially in congested networks. In this paper, we design an efficient backoff algorithm that utilizes a reinforcement learning method to determine optimal values of backoffs. The mobile nodes share a common contention window (CW) in our scheme, and using a Q-learning algorithm, they can avoid collisions by finding and implicitly reserving their optimal time slot(s). In addition, we introduce Frame Size Control (FSC) algorithm to minimize the possible degradation of aggregate throughput when the number of nodes exceeds the CW size. Our simulation shows that the proposed backoff algorithm with FSC method outperforms the 802.11 protocol regardless of the traffic conditions, and an analytical modeling proves that our mechanism has a unique operating point that is fair and stable.

• Ocean Systems Engineering
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• 제1권3호
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• pp.249-261
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• 2011

The stochastic nonlinear dynamic behavior and probability density function of ship rolling are studied using the nonlinear dynamical systems approach and probability theory. The probability density function of the rolling response is evaluated through solving the Fokker Planck Equation using the path integral method based on a Gauss-Legendre interpolation scheme. The time-dependent probability of ship rolling restricted to within the safe domain is provided and capsizing is investigated from the probability point of view. The random differential equation of ships' rolling motion is established considering the nonlinear damping, nonlinear restoring moment, white noise and colored noise wave excitation.

• 대한원격탐사학회지
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• 제40권2호
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• pp.141-150
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• 2024

Ground subsidence in urban areas is mainly caused by anthropogenic factors such as excessive groundwater extraction and underground infrastructure development in the subsurface composed of soft materials. Global Navigation Satellite System data with high temporal resolution have been widely used to measure surface displacements accurately. However, these point-based terrestrial measurements with the low spatial resolution are somewhat limited in observing two-dimensional continuous surface displacements over large areas. The synthetic aperture radar interferometry (InSAR) technique can construct relatively high spatial resolution surface displacement information with accuracy ranging from millimeters to centimeters. Although constellation operations of SAR satellites have improved the revisit cycle, the temporal resolution of space-based observations is still low compared to in-situ observations. In this study, we evaluate the extraction of a time-series of surface displacement in Incheon Metropolitan City, South Korea, using the small baseline subset technique implemented using the commercial software, Gamma. For this purpose, 24 COSMO-SkyMed X-band SAR observations were collected from July 12, 2011, to August 27, 2012. The time-series surface displacement results were improved by reducing random phase noise, correcting residual phase due to satellite orbit errors, and mitigating nonlinear atmospheric phase artifacts. The perpendicular baseline of the collected COSMO-SkyMed SAR images was set to approximately 2-300 m. The surface displacement related to the ground subsidence was detected approximately 1 cm annually around a few Incheon Subway Line 2 route stations. The sufficient coherence indicates that the satellite orbit has been precisely managed for the interferometric processing.

• BMB Reports
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• 제54권10호
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• pp.489-496
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• 2021

Chromatin has highly organized structures in the nucleus, and these higher-order structures are proposed to regulate gene activities and cellular processes. Sequencing-based techniques, such as Hi-C, and fluorescent in situ hybridization (FISH) have revealed a spatial segregation of active and inactive compartments of chromatin, as well as the non-random positioning of chromosomes in the nucleus, respectively. However, regardless of their efficiency in capturing target genomic sites, these techniques are limited to fixed cells. Since chromatin has dynamic structures, live cell imaging techniques are highlighted for their ability to detect conformational changes in chromatin at a specific time point, or to track various arrangements of chromatin through long-term imaging. Given that the imaging approaches to study live cells are dramatically advanced, we recapitulate methods that are widely used to visualize the dynamics of higher-order chromatin structures.

• 한국유체기계학회 논문집
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• 제3권4호
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• pp.15-21
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• 2000

An anemometer employing the bulk PTC thermistor as the sensing element is investigated in this study. The numerical and experimental works are carried out to improve the sensitivity problem of the element by focusing fluid dynamics point of view. The typical shape of the sensing element has been used as a rectangular type, but this shape has a sensitivity problem because of flow separations on the sharp edge when the flow direction is different from that of the sensing element. In order to reduce the reading error, the installer has to be very careful about the flow direction. The reading error fluctuation by time as well as the sensitivity problem can be improved considerably through this study. It can be concluded that the small change of the sensor shape can improve the performance of the flow sensor.

• 대한임베디드공학회논문지
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• 제12권1호
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• pp.27-34
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• 2017

The signal transmitted from radar is not reflected from a single point when the signal reflected by complex target. Resultantly, the amplitude and phase of the received signal can be changed because the target has lots of scatterers. The changes of the amplitude and the phase mean Glint and RCS, respectively. Although the Glint and RCS that caused by the same scatters are uncorrelated, however, they are not independent completely. Therefore, this paper proposes a method for generating the Glint and RCS by using same random number generator. And the time correlations of the Glint and RCS are respectively implemented in frequency domain by using each power spectral density of them.

• Management Science and Financial Engineering
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• 제19권2호
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• pp.49-53
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• 2013

A Markov-chain Monte Carlo sampling algorithm samples a new point around the latest sample due to the Markov property, which prevents it from sampling from multi-modal distributions since the corresponding chain often fails to search entire support of the target distribution. In this paper, to overcome this problem, mode switching scheme is applied to the conventional MCMC algorithms. The algorithm separates the reducible Markov chain into several mutually exclusive classes and use mode switching scheme to increase mixing rate. Simulation results are given to illustrate the algorithm with promising results.