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Necrotizing Enteritis Caused by Pharyngostomum cordatum Infection in a Stray Cat

  • Kim, Ji-Hyeon;Lee, Kyunghyun;Sohn, Woon-Mok;Kim, Ha-Young;Lee, Yu-Ran;Choi, Eun-Jin;So, ByungJae;Jung, Ji-Youl
    • Parasites, Hosts and Diseases
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    • v.57 no.1
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    • pp.17-20
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    • 2019
  • A stray female cat of unknown age, presenting bright red watery diarrhea, was submitted to the Animal and Plant Quarantine Agency for diagnosis. In the small intestines extracted from the necropsied cat, numerous white oval-shaped organisms were firmly embedded in the mucosa and there was thickening of intestinal wall. Histopathological analysis revealed severe necrotizing enteritis, together with atrophied intestinal villi, exfoliated enterocytes, and parasitic worms. Recovered worms were identified as Pharyngostomum cordatum by morphological observation and genetic analysis. Although P. cordatum is known to occur widely in Korea, this is the first clinical description of an infection by P. cordatum causing severe feline enteritis.

Analysis of Antioxidant Enzyme Activity During Seedling Growth of Leymus chinensis Trin Under Salt and Dehydration Stresses (고염과 건조 스트레스 처리 조건 동안 양초 유식물체의 항산화효소 활성 분석)

  • Shim, Donghwan;Nam, Ki Jung;Kim, Yun-Hee
    • Journal of Life Science
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    • v.28 no.7
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    • pp.772-777
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    • 2018
  • To understand the adaptability of Leymus chinensis forage grass to environmental stresses, we analyzed the $H_2O_2$ scavenging activity based on several antioxidant enzymes and total phenolics content, including peroxidase (POD), ascorbate peroxidase (APX), and catalase (CAT), in shoots and roots subjected to salt and dehydration stresses during seedling growth. After NaCl or PEG treatment, plants showed reduced seedling growth under over 200 mM NaCl or 30% PEG treatment condition in shoots and roots compared with the control condition. In addition, plants showed high enzymatic activity of CAT in the shoots, whereas they exhibited high activity levels of APX and POD in the roots in both the NaCl and PEG treatment conditions. These results seem to indicate that Leymus chinensis seedlings responding to salt and dehydration stresses during initial growth is associated with enhanced activity of $H_2O_2$ scavenging antioxidant enzymes in the shoots or roots. The plants also showed high levels of total phenolics under NaCl treatment, with a high concentration in both the shoots and roots. Our results showed that the induced activity patterns of APX in the roots and CAT in the shoots indicate that low $H_2O_2$ levels were mainly maintained through tissue-specific redox homeostasis involving enzymes such as APX and CAT during salt and dehydration stresses. This study highlights the importance of antioxidant enzymes in the establishment of Leymus chinensis seedlings under high salinity conditions, such as typical desertification.

Validity of the New Caries Activity Test using Real-Time Polymerase Chain Reaction (실시간 중합효소연쇄반응 방법을 이용한 새로운 치아우식 활성 검사법의 유효성)

  • Kwon, Doyoun;Kim, Heejin;Nam, Okhyung;Kim, Misun;Choi, Sungchul;Kim, Kwangchul;Lee, Hyoseol
    • Journal of the korean academy of Pediatric Dentistry
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    • v.45 no.3
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    • pp.354-362
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    • 2018
  • Periogen is a new caries activity test using real-time polymerase chain reaction. The aim of this study was to assess the validity of Periogen by evaluating the correlation with dmft, dmfts indices and comparing with Cariview and caries risk assessment tool (CAT). 83 children under 6 participated in this study. Dmft, dmfts indices and CAT were collected through an examination of oral health status. Plaque samples for Periogen and Cariview were collected and manipulated according to the manufactures' instructions. The correlation coefficient of Periogen, Cariview and CAT with the dmfts index were 0.38, 0.56 and 0.66 in each (p < 0.01). The sensitivity of Periogen, Cariview and CAT were 43%, 76% and 95% and specificity were 80%, 72% and 74% respectively. Area under curve under the receiver operating characteristic curves in each method indicated 0.69, 0.81 and 0.85. CAT and Cariview were more effective in evaluation the risk of dental caries than Periogen so far. To be used Periogen clinically, more improvements for higher validity were needed.

Determination for Inflow Routes of Insects Caused by Manufacturing and Drinking Process in Korean Rice-wine (한국 전통약주에서 제조 및 음용 과정 중 발생하는 곤충의 혼입 경로에 대한 판정)

  • Kim Seung-jin;Lee Jeoung-hoon;Choi Yuung-hwan;Kim Gye-won
    • Journal of Food Hygiene and Safety
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    • v.20 no.3
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    • pp.123-127
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    • 2005
  • To determine the possibility of inflow routes for insect in Korean rice-wine, we investigated catalase (CAT) activity and oxygen bubble formation through stereoscopic microscope in pasteurized insects (bee, fly, fruit fly) treated with $H_2O_2$. The pasteurization condition was 30 and 60 min heating at 65 and $70^{\circ}C$. Bubble was not shown under the CAT level of 50 $\mumoles/min/ml$. CAT activity level was more sensitive compared with oxygen bubble formation, but the CAT activity had correlation with oxygen bubble formation method. We also tested bubble formation at room temperature, 65 and $70^{\circ}C$ for 30 days. The bubble formation was slowly decreased in all insects at room temperature during experiment, but it was rapidly decreased at 65 and $70^{\circ}C$. The fruit fly was not shown bubble formation at 65 and $70^{\circ}C$. These results suggest that bubble farmation method was a new simple method for inflow routes of insects caused by manufacturing and drinking process in pasteurized Korean rice-wine.

The Effects of the Makgeolri on the Antioxidative Activity in the Endotoxin LPS-treated Rats (내독소인 LPS로 처치된 흰쥐에 대한 막걸리 항산화 활성효과)

  • Kwon, Ryun-Hee;Chae, Go-Yeon;Ho, Boe-Jin
    • Journal of Food Hygiene and Safety
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    • v.26 no.2
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    • pp.166-170
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    • 2011
  • Modem people have begun to have the nationwide interest in the rice wine called Makgeolri which is one of the traditional Korean alcoholic liquors. This study was performed to investigate the effects of San sung Makgeolri extract (SM) on antioxidation together with the determination of pH and dissolved oxygen (DO) in the progress of fermentation in the lipopolysaccharide(LPS)-treated rats. We examined the levels of SOD (superoxide dismutase), CAT (catalase), GPx (glutathione peroxidase) in liver homogenates and the histopathological observations in liver tissue. LPS-treated group markedly decreased the levels of SOD, CAT and GPx. But SM + LPS-treated group significantly increased the levels of them. Furthermore, the antioxidative effects of SM were supported by the histopathological observations in liver tissue which showed severe inflammation and necrosis in LPS-treated group, compared to the attenuated inflammation and necrosis in SM + LPS-treated group. This results suggested that SM could be a candidate of antioxidative material in spite of alcoholic liquors.

Radiographic Diagnosis of Esophageal Obstruction by Persistent Right Aortic Arch in a Kitten (어린 고양이에서 우대동맥궁잔존에 의한 식도폐색의 방사선학적 진단)

  • 이기창;이희천;정성목;권오경;최민철
    • Journal of Veterinary Clinics
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    • v.20 no.2
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    • pp.248-251
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    • 2003
  • Esophageal obstruction as a result of persistence of the right aortic arch was diagnosed in a 3-month-old male Persian cat. Regurgitation right after weaning and retardation were noted in this cat. Survey radiographic signs on the lateral view include ventral deviation of the thoracic trachea caused by draping of the dilated esophagus over the dorsum of the trachea and a distinct interface of the dorsal wall of the esophagus silhouetting with the cranial thoracic hypaxial muscles. On the ventrodorsal view, the cranial mediastinum was widened with soft tissue density. The trachea was deviated to the right. In an esophagram, the segmental dilation of the esophagus with constriction of the lumen just cranial to the heart base was apparent. Thoracotomy was performed and corrective surgery was carried out. At surgery, it was confirmed that the esophagus was constricted at the cranial to the base of the heart.

Acquired myasthenia gravis in a domestic shorthair cat with cranial mediastinal mass

  • Song, Doo-Won;Kang, Min-Hee;Park, Hee-Myung
    • Korean Journal of Veterinary Research
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    • v.56 no.2
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    • pp.121-123
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    • 2016
  • A 7-year-old castrated male domestic short-hair cat presented with anorexia, constipation, depression, and voice alteration. Physical and neurological examinations revealed hyperthermia ($40.5^{\circ}C$), ventroflexion of the neck, reduced responses to external stimuli, generalized muscle weakness, and exercise intolerance. Thoracic radiographs revealed the presence of a cranial mediastinal mass. The history, clinical signs, and other examination results were compatible with acquired myasthenia gravis (MG). Acetylcholine receptor (AChR) antibody titers were determined to confirm MG and the serum AChR antibody concentration was 1.24 nmol/L (reference interval, < 0.3 nmol/L). This is the first diagnosis of acquired MG in a cat in Korea.

Partial Pancreatectomy Using Ultrasonic Scalpel for Exocrine Pancreatic Ductal Papillary Carcinoma in a Cat

  • Park, Jiyoung;Lee, Young Won;Lee, Hae-Beom;Jeong, Seong Mok
    • Journal of Veterinary Clinics
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    • v.35 no.6
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    • pp.294-298
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    • 2018
  • This report presents a rare case of feline exocrine pancreatic ductal carcinoma including treatment and prognosis. A 4.6-year-old castrated male American Shorthair cat, weighing 5 kg was presented with intermittent vomiting, diarrhea and loss of appetite. Through physical examination and radiography, a cranial abdominal mass was identified. Exploratory laparotomy was carried out. The largest mass was connected to the left lobe of the pancreas, and there were several small, nodular masses scattered along the abdominal lining. The pancreatic mass was removed using an ultrasonic scalpel, and the patient recovered favorably. Histopathologically, the resected lesion was diagnosed as an exocrine pancreatic ductal papillary carcinoma. The patient was maintained with conservative therapy and euthanized on post-operative day 262. Partial pancreatectomy using an ultrasonic scalpel was accomplished safely without evidence of pancreatic leakage.

Clinical evaluation and surgical intervention for diaphragmatic eventration mimicking peritoneopericardial hernia in a cat

  • Min-Jung Ko;Kyoung-Min Song;Hun-Young Yoon
    • Journal of Veterinary Science
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    • v.25 no.2
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    • pp.19.1-19.6
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    • 2024
  • A 2-year-old spayed female British Shorthair cat presented with an increased frequency and duration of cough since infant period. Based on radiographic, ultrasonographic, and computed tomography findings, peritoneopericardial diaphragmatic hernia was considered so that repair surgery was planned. During celiotomy, lax diaphragm was identified instead of defect. Transabdominal diaphragmatic plication was performed to resolve lax diaphragm and to prevent recurrence by overlapping relatively normal part of diaphragm. Diagnosed with diaphragmatic eventration postoperatively, the cat showed improvement in clinical signs and imaging results. Transabdominal diaphragmatic plication is a suitable treatment; the patient maintained normally during a 14-month follow-up period.

CNS Relapsed T-cell Lymphoma in a Young Cat (어린 고양이에서 발생한 중추신경계로 재발한 T세포 림프종)

  • Seo, Kyoung-Won;Oh, Ye-In;Han, Sei-Myoung;Go, Du-Min;Lee, Jeong-Ha;Youn, Hwa-Young
    • Journal of Veterinary Clinics
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    • v.31 no.3
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    • pp.226-232
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    • 2014
  • An 8-month-old domestic shorthair cat presented with decreased activity and anorexia. Diagnostic imaging revealed cranial mediastinal mass and enlarged mesenteric lymph nodes. Fine needle aspirates showed a marked increase in malignant lymphocytes. Multicentric lymphoma (stage V-b) was diagnosed. The cat treated with COP protocol chemotherapy, and complete remission was induced. CNS relapse developed 314 days after the initiation of chemotherapy. Treatment with rescue protocol greatly reduced the clinical signs for a short period. The cat was in partial remission for 33 days and overall survival time was 383 days. Multicentric T-cell lymphoma with brain involvement was confirmed after necropsy by histopathology and immunohistochemistry.