• 아시안잔디학회지
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• 제11권1호
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• pp.73-85
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• 1997

Although zoysia grass (Zoysia spp.) has a lot of excellent chracteristics as warm-season grass, it have been limited in use due to slow establishment, low seed production, poor shade tolerance and other factors. Breeding trials have been continued from 1900's, much attentions have been paid especially in U.S.A., Korea and Japan recently. In U.S.A., more than 24 varieties had been evaluated at National Turfgrass Evaluation Program(NTEP) from 1991 to 1995 and some were regsistered as commercial. After the 6th International Turfgrass Research Conference at Japan in 1989, Japan Turfgrass In-corporation (JTI) sponsored by private companies and government carried out breeding programs for pest, salt, and shade tolerant and herbicide resistant varieties. JTI also has been trying to im-prove vigor and breed evergreen zoysia Korean breeders collected germplasms since 1960's. After USDA breeders came to Korean penesula in 1982, Korean breeders joined with USDA zoysia breeding project for several years. Many interspecific hybrids and natural selected varieties were breeded that period both in U.S.A and Korea. Breeding objectives were to extend green color period, improve leaf quality and density, and better leaf color at dormant stage. Since 1990's, zoysia grass breeding trials are getting more diverse in many points such as random amplified polymorphic DNA (RAPD) assay for ecotype identification. The objectives of this study are to evaluate germplasms in Korea, and also review the present status and future prospect in zoysia grass breeding in the world.

• Journal of Microbiology and Biotechnology
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• 제21권4호
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• pp.333-340
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• 2011

Herbicide-tolerant Zoysia grass has been previously developed through Agrobacterium-mediated transformation. We investigated the effects of genetically modified (GM) Zoysia grass and the associated herbicide application on bacterial community structure by using culture-independent approaches. To assess the possible horizontal gene transfer (HGT) of transgenic DNA to soil microorganisms, total soil DNAs were amplified by PCR with two primer sets for the bar and hpt genes, which were introduced into the GM Zoysia grass by a callus-type transformation. The transgenic genes were not detected from the total genomic DNAs extracted from 1.5 g of each rhizosphere soils of GM and non-GM Zoysia grasses. The structures and diversities of the bacterial communities in rhizosphere soils of GM and non-GM Zoysia grasses were investigated by constructing 16S rDNA clone libraries. Classifier, provided in the RDP II, assigned 100 clones in the 16S rRNA gene sequences library into 11 bacterial phyla. The most abundant phyla in both clone libraries were Acidobacteria and Proteobacteria. The bacterial diversity of the GM clone library was lower than that of the non- GM library. The former contained four phyla, whereas the latter had seven phyla. Phylogenetic trees were constructed to confirm these results. Phylogenetic analyses of the two clone libraries revealed considerable difference from each other. The significance of difference between clone libraries was examined with LIBSHUFF statistics. LIBSHUFF analysis revealed that the two clone libraries differed significantly (P<0.025), suggesting alterations in the composition of the microbial community associated with GM Zoysia grass.

• 한국작물학회지
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• 제66권3호
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• pp.265-269
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• 2021

본 연구는 새로운 종자형 잔디 개발을 위한 유전자원의 분석을 통해 기초자료를 제공하고자 수행되었다. 우리나라에 자생하고 있는 조이시아그래스종 잔디는 주로 포복경이나 지하경을 통해 번식하며, 자연적으로 발아가 잘 되지 않아 종자형 육종을 잘 이루어지지 않고 있었다. 하지만 종피 처리 기술이 개발된 이후, 종자형 잔디 개발이 이루어지고 있다. 종자형 잔디를 개발하기 위해 확보하고 있는 잔디 유전자원에서 꽃대 형성률을 확인할 필요성이 생겼으며, 이 실험에서 549개 라인을 확인해 본 결과, 43개 라인, 즉 7.83%의 라인에서만 꽃대가 출현한 것으로 확인할 수 있었다. 상관분석을 실시 해 본 결과, 이식 후 생존률과 포복경 생성률은 유의하게 양의 상관관계(Rho = 0.44)를 보였고, 이러한 경향은 20% 이상 꽃대를 형성한 것으로 나타난 라인에서 상관관계가 더 높은 경향(Rho = 0.55)을 보였다. 하지만 이식 후 생존률과 포복경 생성률은 꽃대 출현과 상관관계가 매우 낮은 것으로 확인할 수 있었고(Rho = -0.11 and Rho = -0.06), 20% 이상의 꽃대 출현을 보인 43개 라인에서는 유의한 결과를 얻지 못하였다. 따라서 종자형 잔디 개발을 위해서는 이식 후 꽃대 출현률이 저조한 것을 감안, 더욱 많은 유전자원을 확보하는 것이 필요하며, 꽃대 출현이 늦게 나타나는 것을 고려하여 이것과 상관관계가 초기 확인 가능한 특질을 찾아, 꽃대 출현을 조기에 예측할 수 있는 기법이 요구된다.

• 아시안잔디학회지
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• 제5권2호
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• pp.95-114
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• 1991

This paper was designed to estimate the interaction of the first productivity, light conditions and to analyze the ecophysiological productivity, growth characteristics grown under various shading conditions in Zoysia japonica . The results summarized this experiment were as follows; 1.After transplanting the grass, increasing rate of leaf number and total leaf length was remarkahly high at the early growth stage in the control and the 30% shading plot. 2.The increasing rate of leaf area ratio (LA R) in all the experimental plot was remarkable high at the early growth stage after transplanting the grass. Especially, the control and 30% shading plot in compared with another plots, were remarkably high at rate of leaf area. 3.The length and the node number of rhizome in the control and 30% shading plot were also high and the difference was significantly large by the growth stage. 4.Chlorophyll content was very high at 36days after transplanting and then decreased. Increasing rate of shade resulted in its low content. The rate of chlorophyll a to b was decreased by in-creasing rate of shade. 5.The content of soluble sugar in the control and 30% shading plot was low at the early growth after transplanting and high at the late growth stage. Its content in the plot of 70% shading was remarkably low throughout the growth stage. 6.The increasing rate of standing plant in all the experimental plots was high at the early growth stage after transplanting. Increasing rate of shade was typically low through all the growth stage.

• 아시안잔디학회지
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• 제2권1호
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• pp.5-30
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• 1988

The object of this experiments was to know the salt tolerance of Fults and other lawn grasses. Fults, Olympic (Tall fescue, Festuca arundinacea Schred), Ceeping red fescue, Kentucky bluegrass and Zoysia grass (Z. japonica S.) were grown in hydroponics with vermiculite at various concentrations of NaCl. Hoagland's solution was used as the basic solution (control), and the concentrations of Cl to it were 1000, 2000, 3000, 4000 and l5OOOppm, respectively. Each was cultivated under the circumstances during 2 months. The results obtained are summarized as follows: 1.The growth of Fults, Olympic, Creeping red fescue and others were better at Cl lOOOppm than control. In the 5OOOppm application, Fescues become worse and 23.9% of them were withered. In concentration of Cl above 9OOOppm, it was impossible to live. 2.In the l0000~l1000ppm application, Olympic and Kentucky bluegrass were become worse and most of them died. 3.Fults were almost possible to live in the below of 9OOOppm, but they began to die in the above of 10000 ppm. 4.With the increasing concentration of Cl, plants were dwarfed and the number of stems, leaves and roots were reduced, but it was especially observed that the number of stolons of Creeping red fescue were increased at 1000~4000ppm. 5.Fults grass was the most salt tolerant turfgrass, but was impossible to live at salt level of about 36 millimhos (Exchange NaCI conductivity-ppm). Among the grasses, according to salt tolerance, they were arranged as follows. Fults > Zoysia japonica S. > Ky belugrass > Olympic grass > Creeping red fescue 6.The number of leaves, stems, tillers, and dry weight of Olympic grass, Fults and others were increased more at Cl 1OOOppm application than control, but in the above 4OOOppm application, those of plants were decreased. 7.The productivity of all grasses under the experiments was increased at 3.l75millimhos (Exchange NaCi conductivity ppm) in the concentration of Cl. The each dry-weight of Olympic, Creeping red fescue, Kentucky bluegrass and Zoysia grass was decreased at 8.85millimhos, and the weight of Fults grass was also decreased remarkably at 12.20millimhos. 8.As the result of this experiments, most plants grow normally at low concentration of NaCI-l000ppm. That seems to stimulate more the grasses to grow than non-salt.

• 한국토양동물학회지
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• 제7권1_2호
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• pp.45-49
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• 2002

골프장에 재식된 들잔디, Zoysia japonica에서 피해 원인을 조사하던 중 뿌리혹선충이 다수 기생하고 있었다. 이 선충의 가해특성과 형태적 특징을 조사하였다. 이 선충은 뿌리에 혹을 형성하지 않으며, 뿌리 겉으로 후반부를 드러낸 암컷은 난낭을 형성하고 있다. M. marylandi의 주요 특징으로는, 암컷의 경우 Perineal 패턴은 둥글며 높게 위치하여 있고, 음문과 항문은 함몰되어 있으며, 물결 모양의 주름은 거칠며 넓게 공간을 형성하고 있다. 유충의 체장은 390.3 $\mu\textrm{m}$ (290-430), 꼬리 길이는 55.1 $\mu\textrm{m}$ (49-59)이며, 4개의 측선을 이룬다 수컷의 구침길이는 18.1 $\mu\textrm{m}$ (15-24), 교접자 길이는 26.2 $\mu\textrm{m}$ (23-29)이며, 4-5개의 측선수를 가진다.

• 아시안잔디학회지
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• 제4권2호
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• pp.125-131
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• 1990

This experiment was carried out in drder to study the changes of morphological characters and thatch weight on N-level and survey times N-levels were 0, 21, 28, 35, 42 and 49 g/m$^2$), respectively. The results are as follows. 1. Thatch weight was significantly different among survey times, and the interaction of survey time N-level. 2. The growth of Zoysia grass and thatch accumulation were incresed by higher N~level The highest value was obtained in all survey times. 3. Most survey characters and thatch accumulation was decreased by growth progressed. 4. Thatch increase rate(TIR) was the lowest at 28g Nm$^2$and T2-T3 period. 5. TIR was the positive significant correlated(P<0.05) with N-level, root weight, clipping weight and thatch weight.

• 아시안잔디학회지
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• 제8권2호
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• pp.101-103
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• 1994

In June 1994, the circular parch of zoysiagrasse(Zoysia japonica) occurred in golf courses in Kyeongnam, Kycooggi province, Korea The causal agent identified as Antonina graminis Maskell (Rhodeagrass scale). A. gramninis settle on a grass leaf shearh and stem, and then suck plants juices, causing daroaged plants to wither and die. Adult scales were legless, immobile, and shell-like protective cover with waxy and 2~Amm in size. Immature scales had three pairs of leg, and were mobile, O.4~ O.9mm in size.

• 아시안잔디학회지
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• 제6권2호
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• pp.89-98
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• 1992

Rhizoctonia oryzae, R. oryzae-sativae 및 R. solani의 균사융합군의 각 균주에 대한 배양특성과 creeping bentgrass 및 들잔디에 대한 병원성과 살균제의 효과에 대하여 조사하였던 바 다음과 같다. 1. 균사생육에 미치는 온도의 영향을 보면 네가지 형으로 나눌 수 있는데, R. oryzae와 R. oryzae-sativae는 생육적온이 $30~35^{\circ}C$인 호고온형이었고, R. solani의 균사융합군의 각 균주도 세가지 형으로 나뉘었는데, 즉 생육적온이 $25~30^{\circ}C$인 고온형, 20~25$^{\circ}C$인 중간형 및 $20^{\circ}C$인 저온형이었다. 2. 접종실험에서 creeping bentgrass에 대해서는 R. oryzae와 R. solani의 AG-1( I A), AG-1( I B), bentgrass분리균 및 벼 분리균이 강한 병원성을 나타냈고, 그 다음으로 AG-2-1, AG-4, AG-5 및 AG-2-2가 중 또는 약한 병원성을 나타냈다. 들잔디에 대해서는 R. oryzae와 R. solani의 들잔디 분리균이 강한 병원성을 나타냈고 AG-1( I B)와 AG-5가 중정도의 병원성을 나타냈다. 3. 살균제의 효과는 creeping bentgrass의 brown patch병과 들잔디의 large patch병에 대해서 capro와 mytan등이 가장 좋았고 brown patch병에 대해서는 thiopan과 pencycuron이, large patch병에 대해서는 tolos와 thiopan이 각각 그 다음으로 좋았다.

• 아시안잔디학회지
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• 제13권1호
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• pp.1-20
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• 1999

This study was carried out to investigate the interaction between productivity and light condition and to analyze the material productivity and productive charactaristics under different hours of light in Zoysia japonica. 1. Rate increasement of leaf number and total leaf length was remarkably high at the early growth stage in the control and 9 hour light treatment. The rate gradually decreased as growth proceeded. But 3 hour treatment was very low in the rate from its early growth stage through the whole test period. 2. The increasing rate of leaf area ratio (LAR) in all the experimental plots was remarkably high at the early growth stage after transplanting the grass. The shorter photoperiod resulted remarkable lower increasement of LAR. 3. The rhizome growth rate of the 9 hour photoperiod was high contrast to the 3 hour photoperiod treatment. The increasing rate of node number was also showed similar trend. 4. Chlorophyll content was very high at the 36 days after transplanting and then decreased gradually. Chlorophyll content on 3 hours photoperiod plot severely decreased. The ratio of chlorophyll a to b was decreased significantly at short day treatment. 5. The content of soluble sugar was increased at shorter photoperiod. The highest ratio of sugar content was on August, 28 by HPLC method. Such a result was attributed to accumulation of sugar in spite of low synthesis of ploysacchrides, translocation by low consumption. 6. The increasing rate of standings in all light treatment was high at the early growth stage after transplanting. Short photoperiod treatment by 3hour showed especially low prganic productivity on Zoysia japonica.