• Applied Microscopy
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• v.44 no.3
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• pp.96-99
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• 2014

Study on the egg envelope of an endemic Korean freshwater fish, Coreoleuciscus splendidus was carried out by light and scanning electron microscopes during its spawning season. The egg envelope on the entire egg is filled with plenty of long cylinder-like villi before and after fertilization. The fertilized eggs consist of two regions, the attaching and non-attaching parts to stick to the substrates. The villi covering the fertilized eggs have three kinds of villi in its length: 1) normal- sized villi of an average of $10.6{\mu}m$ on the non-attachment part; 2) longer and more slender villi over about $20{\mu}m$ on the attachment part, at least being two times longer than those of the non-attachment; and 3) shorter villi under about $5.0{\mu}m$ around the micropyle, with half the size of the non-attachment villi. The micropyle rotated in a counter-clockwise direction with five pieces of the ridges.

• The Korean Journal of Zoology
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• v.36 no.2
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• pp.304-318
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• 1993

1991년 8월부터 그이듬해 7월까지 채취된 무지개 송어(Oncorhvnchus mykiu) 난모세포의발달 단계를 이해하기 위하여 광필 및 전자현미경을 이용하여 본 각구를 실시하였다. 건국 대학교 축산대학 양수.장에서 수온이 14-16$^{\circ}C$이고 용존산소량이 7.6 $\pm$ 0.3ppm. PH 5.8 $\pm$ 0.2인 자연채광 상태인 1개의 10m3 탱크에서 N R.C. 사양표준에 준한 펠렛트 배합사료를 급여하여 연중 사육중인 체중 700-1,2009의 한복새끼인 무지개 송어 암컷 120마리를 공시희.로 이용 하였다. LPO(late perinucleolus oocyte), EMO(early maturing oocyte) 단계에서 과립막 세포와 협막세포와 같은 난포막 세포는 단층구조로 부터 입 방형구조로 변화되었고. 완전한 성장, 성숙 및 과숙기에 방사대(Eons rsdiat3)에 있는 pore canals의 입구가 봉쇄되었다. 방사대는 맨바깥목에 균일하면서 점은 두께의 막과 나선형의 두꺼운 복의 2개의 막으로 구성되어 있으며. 성숙기에 있는 난모세포에는 수초 및 자갈에 붙을 수 있는 온수성 어종 보다 상대적으로 얇은 점액성의 막이 그 주위를 둘러싸고 있다. 난모세포가 성장함에 따라 세포질 주변부 및 임립등 세포내에 막대기 형태의 미토콘드리아와 비대해진 내형질세강의 육가 급증하였다. 전자밀도가 높은 소포는 방사대에 인접한 난세포질 주변부에 많이 존재하며. 이는 성장중인 방사대의 pore Canals을 통해서 간으로 부터 합성된 단백질과 탄수화물을 수송하는 것과 밀접한 관계가 있는 것으로 사료된다. 자연적인 조건하에서 fP포막은 난형성 및 이에 영향을 미치는 성호르몬의 발달에 충분한 대르몬을 계절에 따라 분비시키는 데에 커다란 영향을 미치는 것으로 나타났다.

• Journal of Marine Life Science
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• v.7 no.1
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• pp.55-59
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• 2022

This study was conducted to provide basic information on the sexual mature and reproductive biology of Trichiurus lepturus. During the oogenesis, size of oocyte and nucleus showed the increase tendency but size ratio of nucleolus to nucleus was decreased. In the result of H-E stain, the stainability of the cytoplasm was changed from basophilic to eosinophilic. The egg diameter of initial vitellogenic oocyte was about 63.2 (±12.7) ㎛. In the cytoplasmic cortex, eosinophilic yolk nucleus was observed. The size of mature oocyte was 216.6 (±24.7) ㎛ and GVBD (germinal vesicle breakdown) was observed. The size of ripe oocyte was 317.9 (±80.9) ㎛ and the thickness of zona radiata was 4.2 (±1.7) ㎛. The oocyte developmental type is considered to belong to the group synchronous type. The accumulation of yolk in the oocyte, like most teleost, is thought to be of two types: exogenous and endogenous accumulation.

• Korean Journal of Animal Reproduction
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• v.16 no.4
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• pp.289-299
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• 1993

The polypeptide patterns of cellular and follicular components were analysed by SDS-PAGE and two dimensional(2-D)electrophoresis combined with isoelectric focusing (IEF) to establish protein profiles in each of the components in porcine follicles. Oocyte-cumulus complexes were cultured in M16+FCS+Gn at 39 in an atmosphere of 5% CO$_2$, in air for 35 h. At the end of the culture, the zona-free oocyte, ZP alone and cumulus cells were prepared and analysed either on 10% SDS-PAGE for the protein profile at the first dimensional gel or 2-D protein pattern. The amounts of each samples were determined for the visualization with Coomasie brilliant blue (CBB) or silver staining, thus giving useful information for the identification of specific proteins in the components or appropriate amount of samples for proper visualization. Oocyte showed 25 and 114 kd major protein band. Other minor components were additionally visualized with CBB on the same gel after silver staining procedure. Cumulus cells also showed specific proteins which is not present in the oocytes. The number of cumulus cell was proper to give major bands with CBB and additional minor bands with silver staining. To establish the degree of contamination from the remnant of the corona radiata to the ZP, zonae were differently prepared or analysed by SDS-PAGE.The preparation of the ZP in this study did not showed any contamination judged by the protein profile of the components. Also follicular fluid showed its specific protein profile without any significant differences among the different sizes of follicles. The established protein profile of each follicular component should be helpful for the identification and elimination of contaminated components, i. e., antigen preparation or immunological studies. The results also suggest that the preparation of each components in the study was appropriate and can be used for a further sensitive biochemical analysis in mammalian oocytes and early embryos.

• The Korean Journal of Zoology
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• v.31 no.4
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• pp.273-282
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• 1988

생쥐에 PMSG와 hOG를 주사한 후 난자-난구복합체의 미세구조의 변화를 환찰함으로세 난구세포의 분산현상을 규명하고자 본 실험을 행하였다. 난자는 PMSG 주사후 48시간까지 별 다른 변화가 없었고 다만 표면막에 miGrOVilli와 Coaled pit의 수가 감소하는 경향을 보였다. 그러나 PMSG-hCG주사 12시간 후에 배란된 난자의 표면은 microvilli와 coated pit가 사라져서 평평하게 되었다. 방사관세포는 PMSG주사 48시간 후메 밀착해 있던 투명대와 간격이 생기기 시작하였고, 투명대를 통관하여 난자의 표면막과 desmosome으로 연결되어 있던 세포질돌기도 퇴화의 징후를 보였다. PMSG-hCG주사 후에는 급속히 격리, 분산되고 세포질돌기는 퇴화하였으며 dermo-some도 사라겼다. 난구세포들은 대조군에서 밀집되어 있었고 거의 gap junction으로 연결되어 있었는데, PMSG주사 24시간 후에는 모양이 등글게 되고 더욱 밀집되었으며, 48시간 후에는 거의 loose junction으로 연결되었고 분산되기 시작하였다. 결국 PMSG-hCG주사 If시간 후에는 완전히 분산되었고 거의 모두 핵응축과 괴사현상을 보였다. 난자- 난구 복합체의 분산은 배란전에 PMSG에 의하여 시작되고 hCG에 의하여 촉진 완결된다는 것이 확실하다. The ultrastructural changes of the oocyte-cumulus complexes of mouse alter injection of PMSG and hOG have been investigated in order to elucidate expansion phenomenon of the cumulus cells. The oocytes until 48 hours after PMSC injection showed no change except a tendency of decrease in numbers of microvilli and the coated pelts on surface membrane. However, surface membrane of the ovulated oocytes 12 hours after PMSC-hCC injection changed to be smooth due to disapperance of microvilli and coated pits. Corona radiate cells tightly attaching to zona pe]lucida 48 hours after PMSC injection began to be detached and their cytoplasmic processes connected by desmosome to oocyte surface membrane showed a degeneration symptom. Thereafter the detachment and degeneration were accelerated by hCG injection and followed by disappearence of desmosome. The cumulus cells in control group were compacted and connected by almost 9aP junction each another. Ite cumulus cells 24 hours after PMSG injection were changed to be round form and more tightly compacted. However, the cumulus cells 48 hours after PMSG injection were connected by almost loose junction and showed the beginning of expansion. Eventuallv, the cumulus cells 12 hours a%or PMSG-hCG injection were completely expanded, and became pvknotic and necrotic in most It is clear that the expansion of oocyte-cumulus complex were initiated by PMSC, then accelerated and completed by hCG before ovulation.

• Applied Microscopy
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• v.32 no.1
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• pp.67-80
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• 2002

This study was carried out to investigate the effects of deer antler extract on the regeneration of peripheral nerves. Sprague-Dawley male rats weighing about 300 gm were fed deer antler extract for 1, 2, and 3 weeks per oral (1.5 ml/100 gm B.W.), respectively, once a day and transected both sides of sciatic nerve of each leg. After keeping for 6 hours, sciatic nerves taken from proximal part of transected region were treated with conventional transmission electron microscopical method and then observed with electron microscope. The results obtained were summarized as follows; 1. Sciatic nerves of normal control group were not showing any sprouts and electron dense axolemmal projections were frequently observed. 2. Sciatic nerves of saline treated groups were showing axonal sprouts at the nodes of Ranvier. The length of them was usually short, and numerous vesicles, vacuoles and organelles including neurofilament were contained. The number of nodes of Ranvier containing sprouts from 100 longitudinal sectioned nerve fibers was 29 (29%) in 1 week treated group, 32 (32%) in 2 weeks treated group, and 30 (30%) in 3 weeks treated group, respectively. 3. Sciatic nerves of deer antler treated groups were showing axonal sprouts at the node of Ranvier as well. Although most of the sprouts were short, some sprouts of 2 weeks and 3 weeks treated groups were quite long. Sprouts usually contained numerous vesicles, vacuoles and cell organelles such as neurofilaments and mitochondria. The number of nodes of Ranvier containing sprouts from 100 longitudinal sectioned nerve fibers was 38 (38%) in 1 week treated group, 46 (46%) in 2 weeks treated group, and 48 (48%) in 3 weeks treated group respectively. The results described above explain pretreatment of deer antler extract improves the sprout formation of transected sciatic nerves, and then it suggests deer antler may be effective for the regeneration of peripheral nerves.