• Journal of Preventive Medicine and Public Health
• /
• v.26 no.1 s.41
• /
• pp.110-125
• /
• 1993

This study was designed to determine whether non-violent criminal and normal control on the basis of concentration of levels of trace mineral and toxic metal by analysis of human scalp hair. The subjects were selected 87 nonviolent criminal from a prison population and 120 normal control from periodic health checks for study. Hair samples were taken from the napes and Minnesota Multiple Personality Inventory (MMPI) was performed also. Five trace mineral (Zn, Cu, Mg, Fe, Na) and two toxic metal (lead, cadmium) contents were determined by an atomic absorption spectrometer. The contents of zinc and magnesium in hair of non-violent criminal were significantly lower than the control group (p<0.01). In the case of lead and cadimum, mean value of criminal group was significantly higher than control group. Significantly higher T-score of MMPI was seen in non-violent criminal group fur psychopathic deviate (Pd), paranoia scale (Pa), and Mania scale (Ma) than control group, but T-score of depression scale (D) was significantly higher in the control group. In the non-violent criminal group, the content of copper inversely proportion to T-score of Hs, D, Hy, Pd, Mf, Pa, Pt, Sc, Si except Ma, also Zinc inversely proportion to T-score of Hy, Mf, Pa, Pt. These results suggest that difference of some hair mineral contents exist between criminal and normal control group. Thus further studies are necessary to determine whether violent and nonviolent criminal group attributed biochemical imbalance with carefully constructed and controlled studies.

• Natural Product Sciences
• /
• v.20 no.3
• /
• pp.160-169
• /
• 2014

44 compounds and 9 minerals were isolated from and detected in the New Zealand deer velvet antler Cervus elaphus var. scoticus L$\ddot{o}$nnberg. The chemical structures of (1 - 26) were identified on the basis of the spectroscopic methods and comparisons with literature, respectively. The structures were identified as cholesterol (CS, 6), 7-keto-CS (7), $7{\beta}$-hydroxy-CS (8), and $7{\alpha}$-hydroxy-CS (9), and included 12 steroid $3{\beta}$-O-(palmitic/stearic/myristic acid esters; PM/SA/MS) [CS-$3{\beta}$-O-PM (1 - 1), CS-$3{\beta}$-O-SA (1 - 2), CS-$3{\beta}$-O-MR (1 - 3), 7-keto-CS-$3{\beta}$-O-PM (2 - 1), 7-keto-CS-$3{\beta}$-O-SA (2 - 2), 7-keto-CS-$3{\beta}$-O-MR (2 - 3), $7{\beta}$-hydroxy-CS-$3{\beta}$-O-SA (3 -1), $7{\beta}$-hydroxy-CS-$3{\beta}$-O-PM (3 - 2), $7{\beta}$-hydroxy-CS-$3{\beta}$-O-MR (3 - 3), $7{\alpha}$-hydroxy-CS-$3{\beta}$-O-SA (4 - 1), $7{\alpha}$-hydroxy-CS-$3{\beta}$-O-PM (4 - 2), and $7{\alpha}$-hydroxy-CS-$3{\beta}$-O-MR (4 - 3)], dinonyl phthalate (5), 8 nucleic acids analogues [uracil (10), deoxyguanosine (11), deoxyuridine (12), uridine (13), deoxyadenosine (14), adenosine (15), inosine (16), and guanosine (17)], and the 9 free amino acids [L-phenylalanine (18), L-isoleucine (19), L-leucine (20), L-tyrosine (21), L-valine (22), L-proline (23), L-threonine (24), L-alanine (25), and L-hydroxyproline (26)]. Also, there are 8 kinds of amino acids [asparagine, serine, glutamine, glycine, histidine, arginine, methionine, and lysine], 2 sialic acids [N-acetylneuraminic acid (27), ketodeoxynonulosonic acid (28)], and 9 minerals [Na > K > Ca > Mg > Fe > Zn > B > Al > Cu] were detected from the autoaminoacid analyzer and ICP spectrometer, HPAEC-PAD/HPLC-FLD, respectively. 9 kinds of oxycholesterol-$3{\beta}$-O-fatty acid ester (2 - 1, 2 - 2, 2 - 3, 3 - 1, 3 - 2, 3 - 3, 4 - 1, 4 - 2, and 4 - 3) and 3 nucleic acids (12, 14, and 15) were isolated from the velvet antler for the first time. 6 kinds of steroids (7, 8, 9, 2 - 1, 3 - 1, and 4 - 1) were examined for their anti-proliferative effects against L1210, P388D1, K562, MEG-01, KG-1, MOLT-4, A549, HepG2, MCF-7, SK-OV-3, and SW-620 cancer cell lines. They showed anti-proliferative effects with $IC_{50}$ values of 0.06, 2.16, 2.42, > 50.0, 1.66 and $8.31{\mu}M$ against L1210, while the values were 24.05, 9.44, 5.22, 0.25. 9.48 and $49.77{\mu}M$ against P388D1, respectively. The others were inactive.

• Applied Biological Chemistry
• /
• v.18 no.4
• /
• pp.194-202
• /
• 1975

This study was conducted to study the properties of the water-soluble natural chelating agents from garbage compost and activated sewage sludge responsible for Fe chelation, which is closely associated with the effectiveness in correcting iron chlorosis in plant. The water-soluble fraction of these materials was fractionated by menas of Sephadex gel filtration and the fractions of Fe chehates were traced by radioactive $^{59}Fe$. The fractions were examined by ultraviolet and infrared. spectroscopy and stability constants for Fe. The water-soluble fraction from garbage compost was separated by Sephadex G-25 into approximately four fractions. Most of the added $^{59}Fe$ was associated with fraction I, which appeared at the void volume. Further fractionation by Sephadex G-50 indicated that the molecular weight of water-soluble chelating agents is in the approximate range of 5000 to 10,000. The water-soluble fraction from activated sewage sludge gave six fractions by Sephadex G-25. Most of the added $^{59}Fe$ was found in the fraction I,II, and III, The molecular weights of most chelating agents associated with $^{59}Fe$ appeared to be less than 5,000 and those of fraction I that appeared at the void volume was in the range of 5,000 to 1,000. Discrepancy between radio activity count and UV absorption indicated the heterogeneity of the fractions obtained by Sephadex gel filtration. Ultraviolet absorption spectra of all fractions separated by Sephadex G-25 and containing chelating agents showed no differences. Fraction IV and V of sewage extract showed absorption maxima and shifting similar to nucleic acid components suggesting the presence of decomposition products of nucleic acid. Similarity fraction VI contained phenolic type amino acid groups. Fraction I of compost extract contained most of the added $^{59}Fe$ and showed weak but extra definite absorption in the 1230, and $1270cm^{-1}$ region, suggesting that extra oxygen groups in polyphenolic structure were probably involved in Fe chelation. In sewage extract, fraction I,II, and III in which most of the $^{59}Fe$ was found, showed strong definite polypeptide absorption in the region of $1540cm^{-1}$ due to NH deformation and C-N stretching of amide groups in the peptidebond. These extra functional groups in fraction I, II, and III appeared to be associated with Fe chelation. The other fractions, not associated with $^{59}Fe$, still have carboxyl and hydroxyl groups, suggesting that these functional groups in these water extracts may not independently form the Fe chelates. Precipitation of ferric hydroxide precluded measuring the stability constants for Fe-chelates. However, the formation constants for Zn chelates as log K values for compost extract and sewage extract at pH 4.0 from which the strength of chelation with Fe could be presumed, were 8.23, and 9.75, respectively, indicating strong complexation with metals. The chelating capacity of compost extract containing 6.5 g organic matter per liter was 0.82 mM, and that of sewage extract containing 5.3 g per liter was 0. 64 mM.

• The Journal of Korean Academy of Prosthodontics
• /
• v.19 no.1
• /
• pp.17-27
• /
• 1981

This study was conducted to determine the chemical composition and the mechanical properties of four commercially available low gold-based crown and bridge alloy produced in Korea. Four dental casting gold-silver-palladium alloys, i.e., A, B, C and D (code of alloys) were selected for the evaluation of chemical composition, ultimate tensile strength, elongation. values and Vickers hardness. The chemical composition of test specimens was analyzed by both emission spectrography and wet gravitation method with a 1.5gm of low gold ingot. The tensile properties and Vickers hardness was determined with cast specimens treated in following three conditions; as-cast, softening heat treatment and hardening heat treatment. The tensile testing bars were cast in accordance with the model designed by Gettleman and Harrison (1969) which was modified from the A. D. A. Specification No. 14 for dental chromium-cobalt casting alloy. Nine tensile test specimens were made from a split silicone mold for each of the test alloys to the size of 2.5mm in diameter and a gauge length of 10mm. All four alloys were handled in accordance with conventional methods used in Type III gold alloys. Ultimate tensile strength and elongation were measured on an Instron Universal Tensile Testing Machine (Model 1125, Japan) operated at a crosshead rate of 0.1cm/min. Elongation values were measured using Digital Measuring Microscope (MS-152, FUSOH, Japan). Vickers hardness was determined with a Vickers Hardness Tester (Model VKH-l, Japan) at a 1.0kg load on a mounted tensile test specimen. The following results were obtained from this study; 1. All tested alloys were composed of Au, Ag, Pd, Cu, Zn and Fe in common. The composition rate of gold for all four alloys was found in the range of $42{\sim}47$ weight % as shown below. Alloy A; Au 45%, Ag 40.2%, Pd 5.76%, others 9.04%. Alloy B; Au 47.1%, Ag 29.03%, Pd 6.98%, others 16.92%. Alloy C; Au 45%, .Ag 26.9%, Pd 6.83%, others 21.07%. Alloy D; Au 41.8%, Ag 34.4%, Pd 6.95%, others 16.85%. 3. The ultimate tensile strength of the four alloys was in the range of $31{\sim}82kg/mm^2$. The test results were shown in the below order from the highest value; As-cast condition; D, B, C, A. Softening heat treament; B, C, D, A. Hardening heat treatment; D, B, C, A. 4. The test :results of the elongation rate for each alloy were in the range of $0.5{\sim}18%$. The test results were shown in the below order from the highest value; As-cast condition; A, D, B, C. Softening heat treatment; A, C, D, B. Hardening heat treatment; C, D, B, A. 5. Vickers hardness for each of the four alloys was in the range of $120{\sim}230$. The test results were shown in the below order from the highest value; As-cast condition; C, B, D, A Softening heat treatment; D, B, C, A. Hardening heat treatment; D, A, C, B. 6. There were no differences in the physical properties between as-cast condition and softening heat treatment.

• Food Science and Preservation
• /
• v.25 no.1
• /
• pp.136-144
• /
• 2018

This study aimed to provide basic evidence regarding the development of materials by analyzing the physicochemical properties and antioxidant activities of Streptococcus thermophilus KCCM 3782 strain-fermented Cordyceps militaris grown on Tenebrio molitor. Among the solvents tested, DPPH radical scavenging activity was the highest in the hot water-extracted sample after 30 min of extraction. Moisture content decreased, whereas crude protein and fat content increased, after lactic acid bacteria-mediated fermentation. Sodium, magnesium, calcium, and zinc contents increased, with potassium levels attaining the maximum value, whereas free amino acid content decreased after the fermentation. Among Hunter's color values, a value increased to $66.7{\rightarrow}149.92$ after fermentation, whereas the L and b values decreased to $15.79{\rightarrow}-15.75$ and $54.45{\rightarrow}0.01$, respectively. Cordycepin content assay increased from 7.02 mg% to 8.66 mg%. The DPPH free radical scavenging activity of the fermented product was 91.92 EDA%, which was higher than that of the extract (84.69 EDA%). The ABTS free radical scavenging and superoxide dismutase (SOD) activities were also higher in the fermented products.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.41 no.2
• /
• pp.205-212
• /
• 2012

This study was performed to investigate the prevalence of metabolic syndrome (MS) and assess nutrient intake levels for the purpose of improving MS risk factors. The participants in this study were 512 adults consisting of 271 men and 241 women aged 30 and over, who visited a public health center for a medical check up. The diagnosis of MS subjects was adapted from the NCEP-ATPIII guidelines and the WHO Asia-Pacific Area criteria for obesity. The MS group was defined as subjects displaying three or more risk factors, and the non MS group was defined as those displaying two or less risk factors. A dietary survey was conducted using the 24-hour recall method. The number of subjects displaying MS syndrome factors was 158 (30.9%), broken down into, 89 men and 69 women. Regarding risk factors in the MS group, the prevalence of waist circumference was 40.5%, hypertension 34.2%, hyperglycemia 31.0%, low HDL-cholesterol 24.7%, and hypertriglycemia 19.6%. BMI, sistolic blood pressure, blood glocose, blood triglyceride, and blood HCL-cholesterol of the MS group were significantly higher compared to the non MS group. Male subjects in the MS group reported high intakes of cereals, sugar, fruits, meat and poultry, oil and fats, and beverages and total food intake was significantly higher compared to the non MS group. Women in the MS group reported high intakes of meat and poultry, milk and dairy products, beverages, and seasonings, and total food intake was higher compared to the non MS group. Dietary diversity score (DDS) was 3.82~4.04, which was not significant among the groups. In men, dietary variety score (DVS) was 16.3 in the MS group and 19.4 in the non MS group, whereas in women, the DVS was 15.2 in the non MS group and 17.0 in the MS group. In GMVDF pattern, 11111 pattern was 30.7%, followed by 01111 for men and 11101 for women. Calorie, fat, and cholesterol intakes in men as well as, calorie, fat, and folate intakes in women in the MS group were higher compared to the non MS group. Intakes of protein, P, Fe, Na, vitamin $B_1$, vitamin $B_2$, niacin, vitamin E, and Zn were higher than the KDRIs. On the other hand, intakes of Ca, K, fiber, vitamin $B_2$, and vitamin C were below the KDRIs. Intakes of lipids, animal food, Na, and cholesterol in the MS group were higher compared to the non MS group, whereas intake of dietary fiber was lower. Our results indicate that continuous, systematic nutritional education program must implemented to reduce the risk factors associated with MS.

• Economic and Environmental Geology
• /
• v.20 no.4
• /
• pp.289-303
• /
• 1987

당(當) 광산(鑛山)은 1936년(年) 금(金), 은(銀) 광종(鑛種)으로 출원(出願)하였다가 1940년(年) 망간을 추가(追加)하여 망간 광산(鑛山)으로 1975년(年)까지 Mn(30~35%) 110,000여(餘)톤을 생산(生産), 국내생산량(國內生産量)의 70%를 점(占)하였고 1976년(年) Mn광상(鑛床) 하부(下部)에 연(鉛), 아연(亞鉛) 유화광(硫化鑛)을 발견(發見), 현재(現在)까지 Pb十Zn=10% 이상(以上) 원광석(原鑛石) 500,000여(餘)톤을 처리(處理), 연정광(鉛精鑛)(Pb : 62%) 37,000여(餘)톤, 아연정광(亞鉛精鑛)(Zn : 46.5%) 37,000여(餘)톤, 유비광정광(硫砒鑛精鑛)(As : 30%) 5,000여(餘)톤을 생산(生産)하였다. 현재(現在) 일처리(日處理) 220톤 선광장(選鑛場)을 일처리(日處理) 400톤 규모(規模)로 증설계획중(增設計劃中)이다. 당(當) 광산(鑛山)에서 현재(現在)까지 시행(施行)한 갱외시추(坑外試錐)는 75개공(個孔) 18,500여(餘)m, 갱내시추(坑內試錐) 750개공(個孔) 40,000여(餘)m 갱도(坑道) 총연장(總延長) 13,000m에 달(達)하며 지표(地表)(623ML)로 부터 수직(垂直) 300m 하부(下部)까지 갱도(坑道)가 개착(開鑿)되어 있다. 당(當) 광산(鑛山)의 지질(地質)은 여러 조사서(調査書)에 의(依)하여 견해(見解) 차이(差異)를 보여주고 있으나 대체(大體)로 다음과 같은 쪽으로 인정되고 있다. 즉(卽) 본지역(本地域) 루층군(累層群)의 층순(層順)을 하위(下位)로 부터 상위(上位)로 향(向)하여 원남층(遠南層)${\rightarrow}$율리통(栗里統)${\rightarrow}$장산규암층(壯山珪岩層)${\rightarrow}$두음리층(斗音里層)${\rightarrow}$장군석회암층(將軍石灰岩層)${\rightarrow}$동수곡층(東水谷層)${\rightarrow}$재산층(才山層)의 순위(順位)로 보며 장산규암층(壯山珪岩層)과 두음리층(斗音里層)을 조선계(朝鮮系)의 양덕통(陽德統)으로, 장군석회암층(將軍石灰岩層)을 대석회암통(大石灰岩統)으로, 동수곡층(東水谷層)과 함탄층(含炭層)인 재산층(才山層)을 평안계(平安系) 지층(地層)으로 대비(對比)한다. 이들은 본지역(本地域) 북(北)쪽에서는 선(先)캠브리아기(紀)의 원남층(遠南層)과 율리통(栗里統)을 불정합(不整合)으로 덮고 남측(南側)에서는 재산층(才山層)과 원남층(遠南層)이 단층접촉(斷層接觸)하고 있다. 이들 지층(地層)의 주향(走向)은 $N60^{\circ}{\sim}80^{\circ}W$, $N60^{\circ}{\sim}80^{\circ}E$이며 경사(傾斜)는 대체(大體)로 $50^{\circ}{\sim}80^{\circ}N$이며 전체적(全體的)으로 역전(逆轉)된 층서(層序)를 보여주는 바 지질구조(地質構造)에 있어서 단사구조(單斜構造)인지 등사(等斜)습곡의 향사(向斜), 또는 등사(等斜)습곡이 배사구조(背斜構造)인지 아직 밝혀지지 않고 있다. 화성암체(火成岩體)는 본지역(本地域) 서측(西側)에 쥬라기(紀) 춘양화강암(春陽花崗岩)이 불규칙(不規則)한 실입(實入) 접촉면(接觸面)을 보여주며 시대미상(時代未詳)(백악기(白堊紀)?)의 거정화강암(巨晶花崗岩), 반화강암(半花崗岩)이 소암주상(小岩株狀)으로 몇 곳 실입(實入)하고 산성(酸性)~중성(中性)의 맥암(脈岩)과 염기성(鹽基性) 안산암질암(安山岩質岩)이 실입(實入)해 있다. 광상(鑛床)은 장군석회암층(將軍石灰岩層)에 배태(胚胎)되어 있는 열수교대(熱水交代) 연(鉛), 아연(亞鉛), 은등(銀等)의 혼합(混合) 유화광상(硫化鑛床)으로 다량(多量)의 Mn분(分)을 수반(隨伴)하며 지표부(地表部)에 Mn광상(鑛床)을 형성(形成)하고 있다. 광상(鑛床)의 형태(形態)는 괴상(塊狀), 각력(角礫)pipe상(狀), 맥상(脈狀)으로 나타난다. 광상(鑛床)의 성인(成因)과 생성시기(生成時期)에 대(對)하여 많은 논란(論難)이 있다. 즉(卽) 열수교대(熱水交代)냐, 접촉교대(接觸交代)냐, 동시퇴적기원(同時堆積起源)이냐, 또는 생성시기(生成時期)가 쥬라기(紀)인지 백악기(白堊紀)인지에 대해 이론(異論)이 있다. 본지역(本地域) 광상(鑛床)은 남본(南本), 100우(右), 북(北), 유비철(硫砒鐵), 동(東), 서(西), 재남(才南), 재동(才東), 110호(號) 등(等)이 지표(地表) Mn로두광화대(露頭鑛化帶)와 관련(關聯) 명명(命名)된 바 전(前)4자(者)는 하부(下部)에서 유화광상(硫化鑛床)이 확인(確認)되었으나 나머지 후자(後者)에서는 아직 하부(下部)에 유화광상(硫化鑛床)이 확인(確認)되지 않고 있으며 남본광상(南本鑛床)으로 부터 남동(南東) 300여(餘)m 지점에 장군석회암층(將軍石灰岩層)과 동수곡층(東水谷層) 경계부(境界部)에 Fe 55~60% 자철광상(磁鐵鑛床)이 확인(確認)된 바 신례미(新禮美) 자철광상(磁鐵鑛床)과 유사성(類似性)이 있는 것 같아 흥미(興味)롭다. 당(當) 광산(鑛山)의 현재(現在)까지의 탐광(探鑛)은 남본광상(南本鑛床) 지표로두(地表露頭)(Mn) 하부(下部)에서 확인(確認)된 연(鉛), 아연(亞鉛), 은(銀) 유화광체(硫化鑛體) 하부(下部)와 전탐(電探)에 의(依)해 확인(確認)된 북광체(北鑛體), 갱도접근중(坑道接近中)에 확인(確認)된 100우광체(右鑛體), 유비철광체(硫砒鐵鑛體) 등(等)의 하부(下部) 탐광(探鑛)을 주(主)로 하고 지표(地表) Mn로두(露頭) 하부(下部)에 대(對)한 시추탐광(試錐探鑛0을 병행(竝行)하고 있으며 시추(試錐)에 의(依)해서 지표(地表)로 부터 790m 하부(下部)(해발(海拔) 200ML)까지 광화대(鑛化帶)가 확인(確認)되었다. 향후(向後) 탐광방침(探鑛方針)을 확고(確固)히 수립(樹立)하기 위(爲)하여는 광상(鑛床)의 성인구명(成因究明)은 물론(勿論) 광상(鑛床)의 배태조건(胚胎條件)에 있어 지질구조규제(地質構造規制)와 화강암(花崗岩)의 실입상(實入狀)과의 관계(關係), 광액(鑛液)의 통로(通路)에 대(對)한 지질구조(地質構造), 모암(母岩)의 화학(化學) 물리적(物理的) 특성(特性)에 대(對)한 연구(硏究) 검토(檢討)가 었어야 하겠다.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.15 no.1
• /
• pp.1-25
• /
• 1982

Limnological study on the physico-chemical properties and biological characteristics of the Lake Ok-Jeong was made from May 1980 to August 1981. For the planktonic organisms in the lake, species composition, seasonal change and diurnal vertical distribution based on the monthly plankton samples were investigated in conjunction with the physico-chemical properties of the body of water in the lake. Analysis of temperature revealed that there were three distinctive periods in terms of vertical mixing of the water column. During the winter season (November-March) the vertical column was completely mixed, and no temperature gradient was observed. In February temperature of the whole column from the surface to the bottom was $3.5^{\circ}C$, which was the minimum value. With seasonal warming in spring, surface water forms thermoclines at the depth of 0-10 m from April to June. In summer (July-October) the surface mixing layer was deepened to form a strong thermocline at the depth of 15-25 m. At this time surface water reached up to $28.2^{\circ}C$ in August, accompanied by a significant increase in the temperature of bottom layer. Maximum bottom temperature was $r5^{\circ}C$ which occurred in September, thus showing that this lake keeps a significant turbulence Aehgh the hypolimnial layer. As autumn cooling proceeded summer stratification was destroyed from the end of October resulting in vertical mixing. In surface layer seasonal changes of pH were within the range from 6.8 in January to 9.0 in guutuost. Thighest value observed in August was mainly due to the photosynthetic activity of the phytoplankton. In the surface layer DO was always saturated throughout the year. Particularly in winter (January-April) the surface water was oversaturated (Max. 15.2 ppm in March). Vertical variation of DO was not remarkable, and bottom water was fairly well oxygenated. Transparency was closely related to the phytoplankton bloom. The highest value (4.6 m) was recorded in February when the primary production was low. During summer transparency decreased hand the lowest value (0.9 m) was recorded in August. It is mainly due to the dense blooming of gnabaena spiroides var. crassa in the surface layer. A. The amount of inorganic matters (Ca, Mg, Fe) reveals that Lake Ok-Jeong is classified as a soft-water lake. The amount of Cl, $NO_3-N$ and COD in 1981 was slightly higher than those in 1980. Heavy metals (Zn, Cu, Pb, Cd and Hg) were not detectable throughout the study period. During the study period 107 species of planktonic organisms representing 72 genera were identified. They include 12 species of Cyanophyta, 19 species of Bacillariophyta, 23 species of Chlorophyta, 14 species of Protozoa, 29 species of Rotifera, 4 species of Cladocera and 6 species of Copepoda. Bimodal blooming of phytoplankton was observed. A large blooming ($1,504\times10^3\;cells/l$ in October) was observed from July to October; a small blooming was present ($236\times10^3\;cells/l$ in February) from January to April. The dominant phytoplankton species include Melosira granulata, Anabaena spiroides, Asterionella gracillima and Microcystis aeruginota, which were classified into three seasonal groups : summer group, winter group and the whole year group. The sumner group includes Melosira granulate and Anabaena spiroides ; the winter group includes Asterionella gracillima and Synedra acus, S. ulna: the whole year group includes Microtystis aeruginosa and Ankistrodesmus falcatus. It is noted that M. granulate tends to aggregate in the bottom layer from January to August. The dominant zooplankters were Thermocpclops taihokuensis, Difflugia corona, Bosmina longirostris, Bosminopsis deitersi, Keratelle quadrata and Asplanchna priodonta. A single peak of zooplankton growth was observed and maximum zooplankton occurrence was present in July. Diurnal vertical migration was revealed by Microcystis aeruginosa, M. incerta, Anabaena spiroides, Melosira granulata, and Bosmina longirostris. Of these, M. granulata descends to the bottom and forms aggregation after sunset. B. longirostris shows fairly typical nocturnal migration. They ascends to the surface after sunset and disperse in the whole water column during night. Foully one species of fish representing 31 genera were collected. Of these 13 species including Pseudoperilnmpus uyekii and Coreoleuciscus splendidus were indigenous species of Korean inland waters. The indicator species of water quality determination include Microcystis aeruginosa, Melosira granulata, Asterionelta gracillima, Brachionus calyciflorus, Filinia longiseta, Conochiloides natans, Asplanchna priodonta, Difflugia corona, Eudorina elegans, Ceratium hirundinella, Bosmina longirostris, Bosminopsis deitersi, Heliodiaptomus kikuchii and Thermocyclops taihokuensis. These species have been known the indicator groups which are commonly found in the eutrophic lakes. Based on these planktonic indicators Lake Ok-Jeong can be classified into an eutrophic lake.

• Applied Biological Chemistry
• /
• v.21 no.3
• /
• pp.150-184
• /
• 1978

Nutritional characteristics and physio-chemical properties of mycelial growth and fruitbody formation of oyster mushroom(Pleurotus ostreatus)in synthetic media, the curtural condition for the commerical production in the rice straw and poplar sawdust media, and the changes of the chemical components of the media and mushroom during the cultivation were investigated. The results can be summarized as follows: 1. Among the carbon sources mannitol and sucrose gave rapid mycelial growth and rapid formation of fruit-body with higher yield, while lactose and rhamnose gave no mycelial growth. Also, citric acid, succinic acid, ethyl alcohol and glycerol gave poor fruit-body formation, and acetic acid, formic acid, fumaric acid, n-butyl alcohol, n-propyl alcohol and iso-butyl alcohol inhibited mycelial growth. 2. Among the nitrogen sources peptone gave rapid mycelial growth and rapid formation of fruit-body with higher yield, while D,L-alanine, asparatic acid, glycine and serine gave very poor fruit-body formation, and nitrite nitrogens, L-tryptophan and L-tyrosine inhibited mycelial growth. Inorganic nitrogens and amino acids added to peptone were effective for fruit-body growth, and thus addition of ammonium sulfate, ammonium tartarate, D,L-alanine and L-leucine resulted in about 10% increase fruit-body yield. L-asparic acid about 15%, L-arginine about 20%, L-glutamic acid, and L-lysine about 25%. 3. At C/N ratio of 15.23 fruit-body formation was fast, but the yield decreased, and at C/N ratio of 11.42 fruit-body formation was slow, but the yield increased. Also, at the same C/N ratio the higher the concentration of mannitol and petone, the higher yield was produced. Thus, from the view point of both yield of fruit-body and time required for fruiting the optimum C/N ratio would be 30. 46. 4. Thiamine, potassium dihydrogen phosphate and magnecium sulfate at the concentration of $50{\mu}g%$. 0.2% and 0.02-0.03%, respectively, gave excellent mycelial and fruit-body growth. Among the micronutrients ferrous sulfate, zinc sulfate and manganese sulfate showed synergetic growth promoting effect but lack of manganese resulted in a little reduction in mycelial and fruit-body growth. The optimum concentrati on of each these nutrients was 0.02mg%. 5. Cytosine and indole acetic acid at 0.2-1mg% and 0.01mg%, respectively, increased amount of mycelia, but had no effect on yield of fruit-body. The other purine and pyrimidine bases and plant hormones also had no effect on mycelial and fruit-belly yield. 6. Illumination inhibited mycelial growth, but illumination during the latter part of vegetative growth induced primordia formation. The optimum light intensity and exposure time was 100 to 500 lux and 6-12 hours per day, respectively. Higher intensity of light was injurous, and in darkness only vegetative growth without primordia formation was continued. 7. The optimum temperature for mycelial growth was $25^{\circ}C$ and for fruit-body formation 10 to $15^{\circi}C$. The optimum pH range was from 5.0 to 6.5. The most excellent fry it-body formation were produced from the mycelium grown for 7 to 10 days. The lesser the volume of media, the more rapid the formation of fruit-body; and the lower the yield of fruit-body; and the more the volume of media, the slower the formation of fruit-body, and the higher the yield of fruit-body. The primordia formation was inhibited by $CO_2$. 8. The optimum moisture content for mycelial growth was over 70% in the bottle media of rice straw and poplar sawdust. 10% addition of rice bran to the media exhibited excellent mycelial growth and fruit-body formation, and the addition of calciumcarbonate alone was effective, but the addition of calcium carbonate was ineffective in the presence of rice bran. 9. In the cultivation experiments the total yield of mushroom from the rice straw media was $14.99kg/m^2$, and from the sawdust media $6.52kg/m^2$, 90% of which was produced from the first and second cropping period. The total yield from the rice straw media was about 2.3 times as high as that from the sawdust media. 10. Among the chemical components of the media little change was observed in the content of ash on the dry weight basis, and organic matter content decreased as the cultivation progressed. Moisture content, which was about 79% at the time of spawning, decreased a little during the period of mycelial propagation, after which no change was observed. 11. During the period from spawning to the fourth cropping about 16.7% of the dry matter, about 19.3% of organic matter, and about 40% of nitrogen were lost from the rice straw media; about 7.5% of dry mallet, about 7.6% of organic matter, and about 20% of nitrogen were lost from the sawdust media. For the production of 1kg of mushroom about 232g of organic matter and about 7.0g of nitrogen were consumed from the rice straw media; about 235g of organic matter and about 6.8g of nitrogen were consumed from the sawdust media, 1㎏ of mushroom from either of media contains 82.4 and 82.3g of organic matter and 5.6 and 5.4g of nitrogen, respectively. 12. Total nitrogen content of the two media decreased gradually as the cultivation progressed, and total loss of insoluble nitrogen was greater than that of soluble nitrogen. Content of amino nitrogen continued to increase up to the third cropping time, after which it decreased. 13. In the rice straw media 28.0 and 13.8% of the total pentosan and ${\alpha}$-cellulose, respectively, lost during the whole cultivation period was lost during the period of mycelial growth; in the sawdust media 24.1 and 11.9% of the total pentosan and ${\alpha}$-cellulose, respectively, was lost during the period of mycelial growth. Lignin content in the media began to decrease slightly from the second cropping time, while the content of reduced sugar, trehalose and mannitol continued to increase. C/N ratio of the rice straw media decreased from 33.2 at spawining to 30.0 at ending; that of the sawdust media decreased from 61.3 to 60.0. 14. In both media phosphorus, potassium, manganese and zinc decreased, at magnesium, calcium and copper showed irregular changes, and iron had a tendency to be increased. 15. Enzyme activities are much higher in the rice straw media than in the sawdust media. CMC saccharifying and liquefying activity gradually increased from after mycelial propagation to the second cropping, after which it decreased in both media. Xylanase activity rapidly and greatly increased during the second cropping period rather than the first period. At the start of the third cropping period the activity decreased rapidly in the rice straw media, which was not observed in the sawdust media. Protease activity was highest after mycelial propagation, after which it gradually decreased. The pH of the rice straw media decreased from 6.3 at spawning to 5.0 after fourth cropping; that of the sawdust media decreased from 5.7 to 4.9. 16. The contents of all the components except crude fibre of the mushroom from the rice straw media were higher than those from the sawdust media. Little change was observed in the content of the components of mushroom cropped from the first to the third period, but slight decrease was noticed at the fourth cropping.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.38 no.10
• /
• pp.1302-1309
• /
• 2009

This study was carried out to investigate physicochemical and functional properties of dried Brassica campestris ssp rapa (BR) sprouts. The proximate compositions of BR sprouts as dry matter basis were 2.35% of moisture content, 22.51% of crude protein, 21.60% of crude lipid, 4.35% of crude ash, and 49.19% of carbohydrate, respectively. The free sugars were identified as glucose and fructose. Analyzing total amino acids, 18 kinds of components were isolated from BR sprouts. The essential amino acid contained in BR sprouts accounted for 47.00% of total amino acid, while the non-essential amino acid accounted for 53.00%. The contents of vitamin A and vitamin E were 0.09 mg% and 3.06 mg%, respectively. Tartaric acid was the major organic acid. Among the minerals in dried BR sprouts, the content of potassium was the highest (882.50 mg%) and those of magnesium and calcium were comparatively high (342.85 mg%, 274.30 mg%). BR sprouts ethanol extract significantly inhibited the HMG-CoA reductase activity in a concentration-dependent manner in vitro. Furthermore, nitrite scavenging ability and DPPH radical scavenging activity of the ethanol extract of BR sprouts were 64.25% and 69.29% at a concentration of 1,000 ${\mu}g$/mL, respectively. These results suggest that BR sprouts possess potential antioxidative capacity and HMG-CoA reductase inhibitory activity.