• Biomolecules & Therapeutics
• /
• v.29 no.6
• /
• pp.685-696
• /
• 2021

In this study, we investigated the inhibitory effect of 5-aminolevulinic acid (ALA), a heme precursor, on inflammatory and oxidative stress activated by lipopolysaccharide (LPS) in RAW 264.7 macrophages by estimating nitric oxide (NO), prostaglandin E2 (PGE2), cytokines, and reactive oxygen species (ROS). We also evaluated the molecular mechanisms through analysis of the expression of their regulatory genes, and further evaluated the anti-inflammatory and antioxidant efficacy of ALA against LPS in the zebrafish model. Our results indicated that ALA treatment significantly attenuated the LPS-induced release of pro-inflammatory mediators including NO and PGE2, which was associated with decreased inducible NO synthase and cyclooxygenase-2 expression. ALA also inhibited the LPS-induced expression of pro-inflammatory cytokines, such as tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6, reducing their extracellular secretion. Additionally, ALA abolished ROS generation, improved the mitochondrial mass, and enhanced the expression of heme oxygenase-1 (HO-1) and the activation of nuclear translocation of nuclear factor-E2-related factor 2 (Nrf2) in LPS-stimulated RAW 264.7 macrophages. However, zinc protoporphyrin, a specific inhibitor of HO-1, reversed the ALA-mediated inhibition of pro-inflammatory cytokines production and activation of mitochondrial function in LPS-treated RAW 264.7 macrophages. Furthermore, ALA significantly abolished the expression of LPS-induced pro-inflammatory mediators and cytokines, and showed strong protective effects against NO and ROS production in zebrafish larvae. In conclusion, our findings suggest that ALA exerts LPS-induced anti-inflammatory and antioxidant effects by upregulating the Nrf2/HO-1 signaling pathway, and that ALA can be a potential functional agent to prevent inflammatory and oxidative damage.

• Journal of Korea Entertainment Industry Association
• /
• v.13 no.8
• /
• pp.181-187
• /
• 2019

The theme of "youth love film" is a strong point in the Korean film industry and has made remarkable achievements in the overseas market. Jae-young Kwak is a famous Korean film director and screenwriter. He is good at shooting "youth love film" with beautiful pictures, exquisite emotions and rich imagination. His films have unique charm and characteristics. This paper expounds the image style, characterization and theme. First of all, from the perspective of image style for this paper, analysis of the film is how to use different narratives and lens language to create a movie's atmosphere, makes the transfer of drunk the oriental beauty. Secondly, the main focus is on the director to find a new way to shape the characters in the film, and pay attention to the description of details, foreshadowing and explaining the plot. Thirdly, based on the theme, this paper analyzes the profound meaning behind romantic films, which reflect the yearning and pursuit of modern people, and people should pay attention to and think deeply. In the modern economic globalization, countries culture mutual exchanges and cooperation, South Korea many outstanding movie worth exploring and research, by studying the Jae-young director Kwak youth love films, excavate its popular film in the market of reason, analysis of the works reflect the aesthetic characteristics, which sums up the experience of the youth love film.