• Asian Pacific Journal of Cancer Prevention
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• v.14 no.4
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• pp.2393-2399
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• 2013

Objective: Mitochondrial DNA (mtDNA) is considered a hotspot of mutations in various tumors. However, the relationship between microsatellite instability (MSI) and mtDNA copy number alterations in lung cancer has yet to be fully clarifieds. In the current study, we investigated the copy number and MSI of mitochondrial genome in lung carcinomas, as well as their significance for cancer development. Methods: The copy number and MSI of mtDNA in 37 matched lung carcinoma/adjacent histological normal lung tissue samples were examined by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) assays for sequence variation, followed by sequence analysis and fluorogenic 5'-nuclease real-time PCR. Student's t test and linear regression analyses were employed to analyze the association between mtDNA copy number alterations and mitochondrial MSI (mtMSI). Results: The mean copy number of mtDNA in lung carcinoma tissue samples was significantly lower than that of the adjacent histologically normal lung tissue samples (p<0.001). mtMSI was detected in 32.4% (12/37) of lung carcinoma samples. The average copy number of mtDNA in lung carcinoma samples containing mtMSI was significantly lower than that in the other lung carcinoma samples (P<0.05). Conclusions: Results suggest that mtMSI may be an early and important event in the progression of lung carcinogenesis, particularly in association with variation in mtDNA copy number.

• Journal of Korean Philosophical Society
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• v.141
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• pp.167-185
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• 2017

Chu Hsi inherited the proposition of Cheng Yi, and it spent him over ten years to finish writing the works of Xi Ming Jie, thus, making the thought of "Li Yi Fen Shu" bethe explanatory model of Xi Ming, therefore, playing the role to determine the tone of Xi Ming. At first, the thought of "Li Yi Fen Shu is a concept to embody the ethical significance of Xi Ming. But in terms of all the discussion about "Li Yi Fen Shu" of Chu Hsi in his life, this proposition is not only for the ethical significance of Xi Ming, but also includes much more general philosophical significance, revealing the general and special relationship of things. The former is the narrow "Li Yi Fen Shu", but the latter is the generalized one. This article won't discuss the generalized one, and it will take the narrow one as the research object. In the past research in academic circles, some scholars thinks that the proposition of "Li Yi Fen Shu" accords with the aim of Xi Ming, some others don't think so. Contrary to both of the two views, this article thinks that there is some conformity and inconformity between the explanatory model of "Li Yi Fen Shu" of Chu Hsi and the aim of Xi Ming. In other words, Contributions and limitations coexist when Chu Hsi explains Xi Ming in the model of "Li Yi Fen Shu", and there is not only the development to the intention of Xi Ming, but alsothe far meaning away from the aim of Xi Ming.