• 한국균학회소식:학술대회논문집
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• 한국균학회 2016년도 춘계학술대회 및 임시총회
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• pp.27-27
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• 2016

The ascomycete fungus Fusarium graminearum is the most common pathogen of Fusarium head blight (FHB), a devastating disease for major cereal crops worldwide. FHB causes significant crop losses by reducing grain yield and quality as well as contaminating cereals with trichothecenes and zearalenone (ZEA) that pose a serious threat to animal health and food safety. ZEA is a causative agent of hyperestrogenic syndrome in mammals and can result in reproductive disorders in farm animals. In F. graminearum, the ZEA biosynthetic cluster is composed of four genes, PKS4, PKS13, ZEB1, and ZEB2, which encode a reducing polyketide synthase, a nonreducing polyketide synthase, an isoamyl alcohol oxidase, and a transcription factor, respectively. Although it is known that ZEB2 primarily acts as a regulator of ZEA biosynthetic cluster genes, the mechanism underlying this regulation remains undetermined. In this study, two isoforms (ZEB2L and ZEB2S) from the ZEB2 gene in F. graminearum were characterized. It was revealed that ZEB2L contains a basic leucine zipper (bZIP) DNA-binding domain at the N-terminus, whereas ZEB2S is an N-terminally truncated form of ZEB2L that lacks the bZIP domain. Interestingly, ZEA triggered the induction of both ZEB2L and ZEB2S transcription. In ZEA producing condition, the expression of ZEB2S transcripts via alternative promoter usage was directly or indirectly initiated by ZEA. Physical interaction between ZEB2L and ZEB2L as well as between ZEB2L and ZEB2S was observed in the nucleus. The ZEB2S-ZEB2S interaction was detected in both the cytosol and the nucleus. ZEB2L-ZEB2L oligomers activated ZEA biosynthetic cluster genes, including ZEB2L. ZEB2S inhibited ZEB2L transcription by forming ZEB2L-ZEB2S heterodimers, which reduced the DNA-binding activity of ZEB2L. This study provides insight into the autoregulation of ZEB2 expression by alternative promoter usage and a feedback loop during ZEA production.

• Journal of Microbiology and Biotechnology
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• 제26권5호
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• pp.967-974
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• 2016

Zearalenone (ZEA) is an estrogenic mycotoxin that is produced by several Fusarium species, including Fusarium graminearum. One of the ZEA biosynthetic genes, ZEB2, encodes two isoforms of Zeb2 by alternative transcription, forming an activator (Zeb2L-Zeb2L homooligomer) and an inhibitor (Zeb2L-Zeb2S heterodimer) that directly regulate the ZEA biosynthetic genes in F. graminearum. Cyclic AMP-dependent protein kinase A (PKA) signaling regulates secondary metabolic processes in several filamentous fungi. In this study, we investigated the effects of the PKA signaling pathway on ZEA biosynthesis. Through functional analyses of PKA catalytic and regulatory subunits (CPKs and PKR), we found that the PKA pathway negatively regulates ZEA production. Genetic and biochemical evidence further demonstrated that the PKA pathway specifically represses ZEB2L transcription and also takes part in posttranscriptional regulation of ZEB2L during ZEA production. Our findings reveal the intriguing mechanism that the PKA pathway regulates secondary metabolite production by reprograming alternative transcription.

• 디지털융복합연구
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• 제20권2호
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• pp.277-287
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• 2022

제로에너지 건축물(ZEB)은 건축물 자체의 에너지 자립도를 높인 건축물로서 에너지를 생산할 수 있는 신재생 요소가 필수적이며, 건물형 태양광(BIPV)이 가장 주목받고 있는 기술이다. ZEB의 설계에서 BIPV은 설계 초기에 계획되어야 하나, 초기 단계에서 BIPV 계획은 미비한 실정이다. 이에 본 연구에서는 설계 초기 BIPV의 계획과 ZEB 자립률 검토를 위해, 3차원 설계와 빅데이터의 융·복합 설계기술인 건축물 정보통합 모델링(BIM)을 기반으로, BIM과 ZEB에 대한 이론적 고찰 및 ZEB 자립률 분석을 위한 요소를 도출하고, BIPV 에너지 생산량과 건물 에너지 소비량 산출 방법을 분석하였다. 최종적으로, 프로젝트 모델에서 에너지 자립률을 산정하고, 등급 기준을 검토함으로써, 설계 초기 ZEB의 에너지 자립률 산정에 대한 기초적인 연구 방법을 제시하였다. 이를 통해, ZEB 주체자의 의사결정을 지원함으로써 설계 생산성을 향상시킬 수 있을 것으로 기대된다.

• 한국건축친환경설비학회 논문집
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• 제12권6호
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• pp.557-566
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• 2018

The ZEB certification system has come into effect since 2018 according to the ZEB road map. From 2020, the public buildings with the total floor area smaller than $3000m^2$ are the target of the certification, and it will be extended to the buildings with up to $5000m^2$ floor area in 2025. However, current mandatory regulations for public office buildings seem already to meet the ZEB certification system planned for 2025. In this work, two buildings belong to $3,000{\sim}5,000m^2$ in total floor area were selected to analyze the possibility of meeting the ZEB certification only by following current obligation regulations. Results showed that the test buildings satisfied the minimum building energy efficiency and energy self-sufficiency rates for the ZEB certification when the mandatory insulation and installation ratio of renewable energy are applied. This can be useful for revising the road map or extending the target buildings of the ZEB certification.

• 한국태양에너지학회 논문집
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• 제39권3호
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• pp.1-7
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• 2019

As part of the government's energy policy, Zero Energy Building certification was launched on January of 2017. However, the three passive-housing rental housing projects are the only ZEB-certified detached housing since the certification's launch. The reason is that, in order for a detached housing to earn ZEB certification, it has to secure self-reliance in energy, and a photovoltaic system is the only viable renewable energy system. Therefore, conducting an analysis to optimize the photovoltaic system in an early design stage is strongly recommended. This study aimed to propose an optimal photovoltaic system design for a detached housing after analyzing through the ECO2 energy simulation of 44 cases, varying in a module type and efficiency, inclination and azimuth. As a result, 15 cases out of 44 cases were analyzed to satisfy ZEB evaluation criteria, and it is thought that these data could contribute greatly to the expansion of ZEB certification dissemination.

• 한국지열·수열에너지학회논문집
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• 제19권1호
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• pp.1-13
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• 2023

Many government in the world have conducted building energy performance certification program to reduce building energy consumption. In this study, a reference building and its HVAC system was modeled, and the energy load and consumption were estimated by the ECO2 program. The software is a simple building energy simulation program based on monthly calculated method. The building energy efficiency rating the the reference building was 1+ under baseline condition. The simulation results showed that the insulation performance slightly affected building energy load and consumption, but light density had a significant effect on them. The application of geothermal heat pumps gave improvement of building energy efficiency rating but it could not make it possible to get zero energy building(ZEB) certification. The ZEB 5 certification could be achieved by using photovoltaics, however getting better grade was difficult. The simulation results showed that the ZEB 4 certification, one grade higher than ZEB 5, could be attained by using more than one renewable energy source such as geothermal and solar energy in this study.

• 한국태양에너지학회 논문집
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• 제30권2호
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• pp.39-45
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• 2010

Zero Emission Building is abuilding which emits virtually '0(zero)' carbon dioxide. Although simple in concept, ZEB requires totally different approach from conventional building in terms of design, engineering, construction and operation. There are few research on ZEB design process as ZEB design requires understanding and knowledge regarding energy and technology. The study aims to propose a design process of Zero Emission Building for architects. The study examined the concept of Zero Emission Building through intensive literature search. The examples of Zero Emission Buildings were investigated, and strategies and technologies applied to the buildings were analyzed. Various conventional design processes were identified and analyzed to examine the applicability to ZEB design, Finally, a new design process which effectively accommodate the requirement of Zero Emission Building was proposed.

• Journal of Genetic Medicine
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• 제16권2호
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• pp.71-75
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• 2019

Periventricular nodular heterotopia (PNH) is a malformation of cortical development in which normal neurons inappropriately cluster in periventricular areas. Patients with Mowat-Wilson syndrome (MWS) typically present with facial gestalt, complex neurologic problems (e.g., severe developmental delay with marked speech impairment and epilepsy), and multiple anomalies (e.g., Hirschsprung disease, urogenital anomalies, congenital heart defects, eye anomalies, and agenesis of the corpus callosum [CC]). MWS is mostly caused by haploinsufficiency of the gene encoding zinc-finger E-box-binding homeobox 2 (ZEB2) due to premature stops or large deletions. We present a case report of a 9-year-old girl with PNH, drug-responsive epilepsy, severe intellectual disability, and facial dysmorphisms only in whom we performed whole-exome sequencing and found a de novo heterozygous missense mutation (c.3134A>C; p.His1045Pro) of ZEB2 (NM_014795.3; NP_055610.1). This mild case of MWS caused by a rare novel missense mutation of ZEB2 represents the first report of MWS with isolated PNH.

• Molecules and Cells
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• 제37권5호
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• pp.383-388
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• 2014

Renal cell carcinoma (RCC) is associated with a high frequency of metastasis and only few therapies substantially prolong survival. Honokiol, isolated from Magnolia spp. bark, has been shown to exhibit pleiotropic anticancer effects in many cancer types. However, whether honokiol could suppress RCC metastasis has not been fully elucidated. In the present study, we found that honokiol suppressed renal cancer cells' metastasis via dual-blocking epithelial-mesenchymal transition (EMT) and cancer stem cell (CSC) properties. In addition, honokiol inhibited tumor growth in vivo. It was found that honokiol could upregulate miR-141, which targeted ZEB2 and modulated ZEB2 expression. Honokiol reversed EMT and suppressed CSC properties partly through the miR-141/ZEB2 axis. Our study suggested that honokiol may be a suitable therapeutic strategy for RCC treatment.

• 교육녹색환경연구
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• 제18권4호
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• pp.58-65
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• 2019

교육시설은 '공공기관 에너지이용 합리화 추진에 관한 규정'에 따라 2014년부터 '건축물에너지효율 1등급 이상'의 의무대상건축물로 지정되었으며, 동 규정에서 정의하는 공공기관 건축물에 해당되어, 2008년 9월 신·재생에너지 의무대상 건축물로 포함되었다. 또한 2019년 4월 녹색건축물 조성 지원법이 개정됨에 따라, 2020년부터 ZEB인증의 의무대상 건축물로 확정되었다. 따라서 본 연구에서는 2015년 2월부터 2019년 5월까지 "건축물에너지효율등급" 본인증을 획득한 316개의 교육시설을 대상으로 "건물에너지효율등급" 및 "신·재생에너지시스템"설치 현황을 파악하고, 에너지 소요량 및 신·재생에너지 생산량을 기준으로 에너지자립률을 분석하였다. 316개 본인증 교육시설의 "건축물에너지효율등급"에 따른 ZEB 인증등급을 추정하면 1+++ 등급 12개 시설의 경우 1곳을 제외하고 모두 ZEB 5등급 이상으로 나타났으며, 239개의 1++ 등급 시설에서 11%에 해당하는 28곳이 ZEB 5등급 수준에 도달하였다. 따라서 현 수준에서 일반 교육시설의 ZEB 인증은 무리가 있으며, 건물에너지효율 1+++등급 상향과 신·재생에너지시스템 투자가 선행되어야 할 것으로 판단된다.