• Journal of Microbiology and Biotechnology
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• 제21권6호
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• pp.637-645
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• 2011

Production of recombinant proteins by excretory expression has many advantages over intracellular expression in Escherichia coli. Hyperexpression of a secretory exoglucanase, Exg, of Cellulomonas fimi was previously shown to saturate the SecYEG pathway and result in dramatic cell death of E. coli. In this study, we demonstrated that overexpression of the PspA in the JM101(pM1VegGcexL-pspA) strain enhanced excretion of Exg to 1.65 U/ml using shake-flask cultivation, which was 80% higher than the highest yield previously obtained from the optimized JM101(pM1VegGcexL) strain. A much higher excreted Exg activity of 4.5 U/ml was further achieved with high cell density cultivation using rich media. Furthermore, we showed that the PspA overexpression strain enjoyed an elevated critical value (CV), which was defined as the largest quotient between the intracellular unprocessed precursor and its secreted mature counterpart that was still tolerable by the host cells prior to the onset of cell death, improving from the previously determined CV of 20/80 to the currently achieved CV of 45/55 for Exg. The results suggested that the PspA overexpression strain might tolerate a higher level of precursor Exg making use of the SecYEG pathway for secretion. The reduced lethal effect might be attributable to the overexpressed PspA, which was postulated to be able to reduce membrane depolarization and damage. Our findings introduce a novel strategy of the combined application of metabolic engineering and construct optimization to the attainment of the best possible E. coli producers for secretory/excretory production of recombinant proteins, using Exg as the model protein.

• Asian-Australasian Journal of Animal Sciences
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• 제27권4호
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• pp.543-550
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• 2014

Fat and fatty acids in muscle and adipose tissues are among the major factors influencing meat quality particularly nutritional value and palatability. The present study was carried out to examine the effects of supplementing inorganic selenium (Se), iodine (I) and a combination of both on fatty acid compositions in serum, and supraspinatus (SS), longissimus lumborum (LL), and semitendinosus (ST) muscles in goats. Twenty-four, 7 to 8 months old, Kacang male goats with a mean live weight of $22.00{\pm}1.17kg$ were individually and randomly assigned into four groups of six animals each for 100 d of feeding prior to slaughter. The animals were offered the same concentrate (basal) diet as 1% of body weight with ad libitum amount of fresh guinea grass. The four groups were as follows: T1 (control) - basal diet without supplementation; T2 - basal diet with 0.6 mg Se/kg DM; T3 - basal diet with 0.6 mg I/kg DM; T4 - basal diet with combination of 0.6 mg Se/kg DM and 0.6 mg I/kg DM. The major fatty acids (FAs) detected in the serum were palmitic (C16:0), stearic (C18:0), oleic (C18:1n9) and linoleic (C18:2n-6), while the major FAs in the selected muscles were C16:0, C18:0 and C18:1n9 acids. The main polyunsaturated fatty acids (PUFA) detected in muscles and serum were (CI8:2n-6), linolenic acid (C18:3n-3), and arachidonic acid (C20:4n-6). No significant differences (p>0.05) were observed in the concentration of total saturated fatty acids (SFA) among the four groups. PUFA concentrations in the goats supplemented with Se (T2) were significantly higher (p<0.05) than the goats of the control group (T1). The PUFA: SFA ratio was significantly higher in the animals supplemented with dietary Se (T2) than those of control ones (T1). It is concluded that dietary supplementation of inorganic Se increased the unsaturated fatty acids in muscle. The supplementation of iodine with or without Se had negligible effects on muscle fatty acid content of Kacang crossbred male goats.

• 대한치과교정학회지
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• 제25권6호
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• pp.675-688
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• 1995

The purposes of this study were:1) to determine the normal range of CR-CO discrepancy in normal occlusion group ; 2) to evaluate the changes of condylar position and craniofacial morphology between centric relation and centric occlusion before and after stabilization splint therapy in malocclusion group outside the normal range of CR-CO discrepancy. The normal occlusion group consisted of 80 subjects who had well-balanced faces and good occlusions with acceptable Class I molar relationship. They had not been treated orthodontically and had no signs or symptoms of temporomandibular joint dysfunction. 71 malocclusion patients enrolled for orthodontic treatment at the Department of Orthodontics, College of Dentistry, Chosun University comprised the malocclusion group, little variation of growth factor by the second molar eruption. They had CR-CO discrepancy beyond normal range and were subdivided into anterior-posteriorly -[25 Class I (0$\geq$4), and 24 Class III (ANB$\leq$0)] : vertically - [20 Normodivergentscy (30$\geq$34), and 18 Hypodivergency (SNGoMe$\leq$30)] ; and sexually - [26 Male and 45 Female]. For malocclusion group, stabilization splint with mutually protected type of occlusal scheme was applied for three months. Panadent articulators, Panadent condylar position indicator (CPI), and lateral headfilm were used to investigate the influence of stabilization splint on condylar position and craniofacial morphology. The results of this study were as follows. 1. The amounts of CR-CO discrepancy in normal occlusion were that the antero-Posterior component (${\bigtriangledown}X$) was $0.56\pm0.46mm$ (Male:$0.63\pm0.42mm$, Female:$0.49\pm0.50mm$) ; the supero-inferior component (${\bigtriangledown}Y$) was $-0.75\pm0.48mm$ (Male:$-0.76\pm0.52mm$, Female:-$0.73\pm0.43mm$) : and the transverse component (${\bigtriangledown}Z$) was $-0.33\pm0.28mm$ (Male : $-0.38\pm0.29mm$, Female:$-0.31\pm0.27mm$). 2. The condylar position was in normal range after stabilization splint therapy. 3. The mandible was always rotated infero-posteriorly after stabilization splint therapy. 4. Antero-posteriorly, Class III malocclusion responded very well to the stabilization splint therapy. 5. Vertically, Hyperdivergency responded very well to the stabilization splint therapy 6. Sexually, Male responded very well to the stabilization splint therapy.

• 한국식품영양과학회지
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• 제23권3호
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• pp.502-508
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• 1994

부산 공동어시장에서 구입된 까치복어, Fugu xanthopterus의 조직부위별 독력을 mouse bioassay법에 의하여 비교 검사하였다. 또한 bio gel P-2 column chromatography으로 복어독을 부분 정제하여, 박층 chromatography, 전기영동, GC-MS 및 HPLC에 의하여 독의 조성을 분석하였다. 즉, 까치복의 개체당 전체 독력은 1,099~167,694 M로서 개체에 따라 뚜렷한 독력 차이가 있었다. 간, 난소 그리고 내장의 평균독력은 각각 $231.0{\pm}51.0,\;175.0{\pm}38.0\;및\;78.8{\pm}16.8MU/g(평균값{\pm}표준오차)$으로 나타난 반면에 근육과 껍질조직의 평균독력은 각각 $3.3{\pm}1.4와\;19.3{\pm}4.3MU/g$으로 개체에 따라 약독 내지 무독인 것으로 나타났다. 또한 각부위별 최고독력의 순서는 간(917MU/g), 난소(459MU/g), 내장(312MU/g), 담즙(101MU/g), 껍질(79MU/g), 정소(72MU/g), 그리고 근육조직 (27MU/g)의 순이었다. 한편, TLC, 전기영동, HPLC로 분석한 결과 복어 개체에 따라 독의 조성이 다름을 알 수 있었고, TTX를 알칼리분해 시켜 생성된 ${C_9}-base$를 TMS화 시켜 GC-MS에 주입시킨 결과 ${C_9}-base$ TMS 유도체의 특성인 406(molecular ion peak), 392(base), 380 및 376 m/z에서 fragment ions에 의해 TTX의 peak는 동일한 mass spectra로 나타났다. 따라서, 이들의 독성에 관한 구체적인 연구자료는 최근들어 수입 복어류의 증가, 양식어업의 발달, 식생활의 다양화 등에 따른 수산 식품의 위생적 안정성을 확보하는데 절실히 필요한 것이라 생각된다.

• Asian-Australasian Journal of Animal Sciences
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• 제22권6호
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• pp.857-864
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• 2009

This experiment was conducted to investigate the effects of interaction between $1{\alpha}$-hydroxycholecalciferol ($1{\alpha}$-OH $D_3$) and phytase on growth performance, parameters of tibia and plasma, and meat quality of 1- to 21-d-old broilers. Two hundred and forty male, 1-d-old Arbor Acres broilers were randomly assigned to 20 cages, with 12 chicks per cage. Five treatments were designed, with four cages each. A 2${\times}$2 factorial experiment was designed to test 0 and 5 ${\mu}g/kg$ of $1{\alpha}$-OH $D_3$ in combination with 0 and 500 U/kg of phytase. A basal diet was formulated to contain 2.9 g/kg of non-phytate phosphorus (NPP), and the control diet was formulated to contain a normal level of NPP (4.5 g/kg). Results showed that $1{\alpha}$-OH $D_3$ alone increased tibia ash, contents of calcium and phosphate, breaking strength, concentrations of plasma calcium and phosphate, and water-holding capacity of breast and thigh meat, while it decreased growth of broilers. Phytase alone improved performance and tibia quality. Although growth of broilers was lower than that of the positive control when the diet was supplemented with $1{\alpha}$-OH $D_3$ and phytase, tibia quality was significantly improved by the addition of $1{\alpha}$-OH $D_3$ and phytase. These data suggest that interaction between $1{\alpha}$-OH $D_3$ and phytase at 2.9 g/kg of dietary NPP could significantly increase bone quality of 1- to 21-d-old broilers, while not improving growth performance.

• 생물정신의학
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• 제20권2호
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• pp.45-53
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• 2013

Objectives Non-major depression with fewer symptoms than required for a Diagnostic and Statistical Manual of Mental Disorders-4th edition diagnosis of major depressive disorder (MDD) has consistently been found to be associated with functional impairment. In this study, we aim to estimate the cognitive impairment and the quality of life in elderly patients with subsyndromal depression (SSD) compared with non-depressive elderly (NDE). Methods The Korean version of Mini International Neuropsychiatric Interview was administered to 194 outpatients with depression and 108 normal controls. SSD is defined as having five or more current depressive symptoms with core depressive symptoms (depressive mood or loss of interest or pleasure) during more than half a day and more than seven days over two weeks. Depression was evaluated by the Korean form of Geriatric Depression Scale of a 15-item short version. Global cognition was assessed by Mini-Mental State Examination in the Korean version of CERAD assessment packet (MMSE-KC). Subjective cognitive impairment was assessed by the Subjective Memory Complaint Questionnaire. Quality of life was evaluated by the Korean Version of Short-Form 36-Item Health Survey. Results The mean score of the MMSE-KC in the SSD group was lower than that in the NDE group with adjustment for age, gender, and education [F = 4.270, p = 0.04, analysis of covariance (ANCOVA)]. If we defined those having Z-score of MMSE-KC < -1.5 as a high risk group of cognitive impairment, the odds ratio for the high risk group of cognitive impairment was 1.86 [95% confidence intervals (CI) 1.04-3.34] in SSD and 7.57 (95% CI 3.50-16.40) in MDD compared to NDE. The scores of physical component summary (F = 9.274, p = 0.003, ANCOVA) and mental component summary (F = 53.166, p < 0.001, ANCOVA) in the SSD group were lower than those in the NDE group with adjustment for age, gender, and education. Conclusions The subjects with SSD, as well as those with MDD, showed impairment of global cognition and also experienced low quality of life in both physical and mental aspects, compared to the NDE group.

• 한국환경농학회지
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• 제32권3호
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• pp.185-192
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• 2013

농촌인구의 감소와 잉여생산작물로 변화된 쌀의 수요에 대비해 최근 20-30년간 휴경논, 즉 묵논이 증가하여 왔으나, 새로운 작물재배지로의 전환 또는 용도지역 변경으로 인한 형질변경이 수반되지 않는 오지의 묵논은 점차 식물생태복원의 주요한 입지로 새롭게 부각되고 있다. 묵논의 입지적, 수리적 여건상 묵밭에 비해 식물의 천이가 활발하고, 습지로서의 기능과 다양한 생물 서식지로서의 기능 등 생태적으로 긍정적인 역할을 담당하게 될 묵논은 연차별로 식물군락의 변화양상이 달라진다. 본 연구에서는 우리나라 전역에 분포하는 묵논을 대상으로 초기, 중기, 후기묵논으로 구분짓고, 각 시기별 식물군락의 변화양상에 대한 식물사회학적 연구가 수행되었다. 강원 경기 5개 권역, 충청남 북 2개 권역, 경상남 북 18개 권역, 전라남 북 10개 권역 등 총 35개 권역을 대상으로 총 203개소의 조사구에서 획득한 식생조사표를 이용하여 군락분류, 군락생태, 군락동태에 대한 비교분석이 이루어졌다. 본 연구를 통해 우리나라 초기, 중기, 후기의 천이단계별 식물군락은 각각 뚝새풀군락, 골풀군락, 버드나무군락으로 특징지어졌으며, 삼림 내 묵논 일부 지역에서 드물게 후기천이 단계에서 오리나무군락이 분포하는 것으로 확인되었다. 이러한 군락의 발달은 대기후적, 지리적 조건과 관계없이 유사한 패턴을 보였다. 묵논식생의 천이단계는 일년생식물기-다년생식물기-관목식물기-교목림기로 발달해가는 천이단계로 구분할 수 있으나 중기묵논에서는 단계별 우점종이 뚜렷이 구분되지 않고 여러 종이 유사한 우점도로 혼생하였다. 군락분류를 통한 우리나라의 연차별 묵논의 식생특성을 분석한 결과, 새로운 생태복원모델로 관심이 높아지는 묵논식생의 변화양상을 파악하였으며, 이러한 결과는 휴경지의 생태복원 및 보전을 위한 기초 자료로써 매우 유효한 정보를 제공하게 될 것으로 기대된다.

• Biomolecules & Therapeutics
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• 제28권2호
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• pp.202-210
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• 2020

Fluoxetine is used widely as an antidepressant for the treatment of cancer-related depression, but has been reported to also have anti-cancer activity. In this study, we investigated the cytotoxicity of fluoxetine to human gastric adenocarcinoma cells; as shown by the MTT assay, fluoxetine induced cell death. Subsequently, cells were treated with 10 or 20 µM fluoxetine for 24 h and analyzed. Apoptosis was confirmed by the increased number of early apoptotic cells, shown by Annexin V- propidium iodide staining. Nuclear condensation was visualized by DAPI staining. A significant increase in the expression of cleaved PARP was observed by western blotting. The pan-caspase inhibitor Z-VAD-FMK was used to detect the extent of caspase-dependent cell death. The induction of autophagy was determined by the formation of acidic vesicular organelles (AVOs), which was visualized by acridine orange staining, and the increased expression of autophagy markers, such as LC3B, Beclin 1, and p62/SQSTM 1, observed by western blotting. The expression of upstream proteins, such as p-Akt and p-mTOR, were decreased. Autophagic degradation was evaluated by using bafilomycin, an inhibitor of late-stage autophagy. Bafilomycin did not significantly enhance LC3B expression induced by fluoxetine, which suggested autophagic degradation was impaired. In addition, the co-administration of the autophagy inhibitor 3-methyladenine and fluoxetine significantly increased fluoxetine-induced apoptosis, with decreased p-Akt and markedly increased death receptor 4 and 5 expression. Our results suggested that fluoxetine simultaneously induced both protective autophagy and apoptosis and that the inhibition of autophagy enhanced fluoxetine-induced apoptosis through increased death receptor expression.

• 한국미생물·생명공학회지
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• 제7권3호
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• pp.135-140
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• 1979

특이하게도 일단 어떤 원인으로든지 변성이 일어나서 침전이 형성되고 난 후에는 그 변성으로 인하여 다시 용해되지 않는 penicillinase를 생성분비하는 Streptomyces 속 균주 AS-727을 토양으로부터 얻었으며, 이 균의 효소생산성과 이 효소의 일반적성질을 검토한 결과는 다음과 같다. 1) 본 효소의 생산을 위해서는 질소원으로서 sodium nitrate, 탄소원으로서 glucose 나 maltose를 사용하여 4 일간 배양하는 것이 바람직했으며 2) 정제는ammonium sulfate로 완전포화시켜 생성된 침전을 원심분리로 모아 그것을 투석해서 조정제효소를 얻는데, 이때 투석할 때 침전은 용해 되지 않는다. 3) 본 효소의 최적 pH는 중성부근 (6.0~8.0)이며 최적온도는 4$0^{\circ}C$ 근처이고, 4) 안정 pH 범위는 4.0~9.0으로서 비교적 넓었으며 열에 대해서는 4$0^{\circ}C$에서는 안정했으며 6$0^{\circ}C$와 8$0^{\circ}C$에서 60 분간 처리하면 각각 약 30%와 40%가 실활되었다. 5) 본 효소의 활성에 있어서 zinc chloride가 $10^{-3}$ M 농도에서 약 20%, $10^{-2}$ M 이상의 고농도에서는 95% 이상을 조해하였다. 6) 그리고 동결이나 명종 고류 또는 유기용매로 일단 형성된 효소단백침전은 다시 용해되지 않는 특이한 성질을 가졌다.

• Journal of Ecology and Environment
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• 제44권1호
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• pp.8-25
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• 2020

Background: Species of the genera Eucalyptus, Cupressus, and Pinus are the most widely planted tree species in the country in general and in Chilimo dry Afromontane forest in particular. Eucalyptus covers 90% of the total planted forest area in the country. However, only limited information exists in the country regarding aboveground biomass (AGB), belowground biomass (BGB), growth, and yield. This study was conducted to assess the variables on 25 and 30 years of age for three planted species: Cupressus lusitanica, Eucalyptus saligna, and Pinus patula in Chilimo plantation forest, in the Central Highlands of Ethiopia. A two-times inventory was conducted in 2012 and 2017. A total of nine square sampled plots of 400 ㎡ each, three plots under Cupressus lusitanica, 3 Eucalyptus saligna, and 3 Pinus patula were used for data collection. Data on height, diameter, soil, and tree stumps were collected. Percent C, % N, and bulk density was performed following chemical procedure. Results: The aboveground biomass ranged from 125.76 to 228.67 t C ha^-1 and the basal area and number of stems from 3.76 to 25.50 ㎡ ha^-1 and 483 to 1175 N ha^-1, respectively. The mean annual basal area and volume increment were between 0.97 and 1.20 ㎡ ha^-1 year^-1 and 10.79 and 16.22 ㎥ ha^-1 year^-1. Both carbon and nitrogen stock of the planted forest was non-significant among the tree species. Conclusion: The aboveground biomass, growth, and yield significantly varied among the species. Cupressus lusitanica had the highest aboveground biomass, volume, and basal area, while Eucalyptus saligna had the lowest value. To a depth of 1 m, total carbon stored ranged from 130.13 to 234.26 t C ha^-1. The total annual carbon sequestration potential was 12,575.18 t CO₂ eq. Eucalyptus has the highest carbon stock density and growth rate than other species.