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Metabolic Flux Distribution for $\gamma$-Linolenic Acid Synthetic Pathways in Spirulina platensis

  • Meechai Asawin;Pongakarakun Siriluk;Deshnium Patcharaporn;Cheevadhanarak Supapon;Bhumiratana Sakarindr
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.9 no.6
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    • pp.506-513
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    • 2004
  • Spirulina produces $\gamma$-linolenic acid (GLA), an important pharmaceutical substance, in a relatively low level compared with fungi and plants, prompting more research to improve its GLA yield. In this study, metabolic flux analysis was applied to determine the cellular metabolic flux distributions in the GLA synthetic pathways of two Spiru/ina strains, wild type BP and a high­GLA producing mutant Z19/2. Simplified pathways involving the GLA synthesis of S. platensis formulated comprise of photosynthesis, gluconeogenesis, the pentose phosphate pathway, the anaplerotic pathway, the tricarboxylic cycle, the GLA synthesis pathway, and the biomass syn­thesis pathway. A stoichiometric model reflecting these pathways contains 17 intermediates and 22 reactions. Three fluxes - the bicarbonate (C-source) uptake rate, the specific growth rate, and the GLA synthesis rate - were measured and the remaining fluxes were calculated using lin­ear optimization. The calculation showed that the flux through the reaction converting acetyl­CoA into malonyl-CoA in the mutant strain was nearly three times higher than that in the wild­type strain. This finding implies that this reaction is rate controlling. This suggestion was sup­ported by experiments, in which the stimulating factors for this reaction $(NADPH\;and\;MgCl_{2})$ were added into the culture medium, resulting in an increased GLA-synthesis rate in the wild type strain.

Interaction of Beef Growth Type${\times}$Production System for Carcass Traits of Steers

  • Brown , A.H. Jr.;Camfield, P.K.;Johnson, Z.B.;Rakes, L.Y.;Pohlman, F.W.;Brown, C.J.;Sandelin, B.A.;Baublits, R.T.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.2
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    • pp.259-266
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    • 2005
  • Steers (n=335) of known genetic backgrounds from four fundamentally different growth types were subjected to two production systems to study differences in carcass traits. Growth types were animals with genetic potential for large mature weight-late maturing, intermediate mature weight-late maturing, intermediate mature weight-early maturing and small mature weight-early maturing. Each year, in a nine-year study, calves of each growth type were weaned and five steers of each growth type were developed on pasture or feedlot and slaughtered at approximately 20 and 14 months of age, respectively. Data collected were pre-slaughter shrunk body weight (SBW); hot carcass weight (HCW); dressing percentage (DRESS); fat thickness at the $12^{th}$ and $13^{th}$ rib interface (FAT); percentage kidney, pelvic, and heart fat (KPH); longissimus muscle area (LMA); marbling score (MARB); quality grade (QG); and yield grade (YG). Year and growth type were significant for all carcass traits. The growth type${\times}$production system interaction was an important source of variation in SBW, HCW; FAT, YG and MARB. The same interaction was non-significant for DRESS, KPH, LMA and QG. Carcass differences in measures of fatness were greater in the feedlot system than in the pasture system. These data could aid producers in matching beef growth type to the production system most suitable for efficient use of resources.

Comparison of Major Black-Box Testing Methods in Object-Oriented Software (객체지향 소프트웨어를 위한 주요 블랙박스 테스트 기법들의 비고)

  • Seo, Kwang-Ik;Choi, Eun-Man
    • Journal of KIISE:Software and Applications
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    • v.33 no.1
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    • pp.1-16
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    • 2006
  • As the size of software is getting huge, it has been impossible for testers to test all of source coed. Therefore, the method which uses information of requirements for testing has been researched and accepted. Although there are a lot of test methods based on requirement specification, each method has a different approach and coverage. So we should consider their various approach and coverage in the stage of test planning. This paper presents the empirical comparison and the result by applying various black-box testing methods to software system. For this experiment we studied five black-box testing methods including UML based specification technique. We applied the five testing methods to application system and evaluated the coverage of extracted test case.

HOT GAS HALOS IN EARLY-TYPE GALAXIES AND ENVIRONMENTS

  • Kim, Eunbin;Choi, Yun-Young;Kim, Sungsoo S.
    • Journal of The Korean Astronomical Society
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    • v.46 no.1
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    • pp.33-40
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    • 2013
  • We investigate the dependence of the extended X-ray emission from the halos of optically luminous early-type galaxies on the small-scale (the nearest neighbor distance) and large-scale (the average density inside the 20 nearest galaxies) environments. We cross-match the 3rd Data Release of the Second XMMNewton Serendipitous Source Catalog (2XMMi-DR3) to a volume-limited sample of the Sloan Digital Sky Survey (SDSS) Data Release 7 with $M_r$ < -19.5 and 0.020 < z < 0.085, and find 20 early-type galaxies that have extended X-ray detections. The X-ray luminosity of the galaxies is found to have a tighter correlation with the optical and near infrared luminosities when the galaxy is situated in the low large-scale density region than in the high large-scale density region. Furthermore, the X-ray to optical (r-band) luminosity ratio, $L_X/L_r$, shows a clear correlation with the distance to the nearest neighbor and with large-scale density environment only where the galaxies in pair interact hydrodynamically with seperations of $r_p$ < $r_{vir}$. These findings indicate that the galaxies in the high local density region have other mechanisms that are responsible for their halo X-ray luminosities than the current presence of a close encounter, or alternatively, in the high local density region the cooling time of the heated gas halo is longer than the typical time between the subsequent encounters.

Near-Five-Vector SVPWM Algorithm for Five-Phase Six-Leg Inverters under Unbalanced Load Conditions

  • Zheng, Ping;Wang, Pengfei;Sui, Yi;Tong, Chengde;Wu, Fan;Li, Tiecai
    • Journal of Power Electronics
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    • v.14 no.1
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    • pp.61-73
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    • 2014
  • Multiphase machines are characterized by high power density, enhanced fault-tolerant capacity, and low torque pulsation. For a voltage source inverter supplied multiphase machine, the probability of load imbalances becomes greater and unwanted low-order stator voltage harmonics occur. This paper deals with the PWM control of multiphase inverters under unbalanced load conditions and it proposes a novel near-five-vector SVPWM algorithm based on the five-phase six-leg inverter. The proposed algorithm can output symmetrical phase voltages under unbalanced load conditions, which is not possible for the conventional SVPWM algorithms based on the five-phase five-leg inverters. The cause of extra harmonics in the phase voltages is analyzed, and an xy coordinate system orthogonal to the ${\alpha}{\beta}z$ coordinate system is introduced to eliminate low-order harmonics in the output phase voltages. Moreover, the digital implementation of the near-five-vector SVPWM algorithm is discussed, and the optimal approach with reduced complexity and low execution time is elaborated. A comparison of the proposed algorithm and other existing PWM algorithms is provided, and the pros and cons of the proposed algorithm are concluded. Simulation and experimental results are also given. It is shown that the proposed algorithm works well under unbalanced load conditions. However, its maximum modulation index is reduced by 5.15% in the linear modulation region, and its algorithm complexity and memory requirement increase. The basic principle in this paper can be easily extended to other inverters with different phase numbers.

Effects of extrusion of rice bran on performance and phosphorous bioavailability in broiler chickens

  • Zare-Sheibani, Ali Akbar;Arab, Masoud;Zamiri, Mohammad Javad;Rezvani, Mohammad Reza;Dadpasand, Mohammad;Ahmadi, Farhad
    • Journal of Animal Science and Technology
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    • v.57 no.7
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    • pp.26.1-26.5
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    • 2015
  • Background: Rice bran is a by-product of the rice-milling process, which remains largely underutilized; however, efficient processing treatments may improve its feeding value for chickens. This is of great economic and environmental importance, as this can lower the production costs, and offer an opportunity for valorization of a low-quality agricultural by-product, to a high-value feed source. Methods: This experiment was conducted to study the effect of extruded rice bran on performance and phosphorous (P) bioavailability in broiler chickens. In a completely randomized design, 200 seven-day-old broilers (Cobb 500) were allotted to five treatments with five replicates per treatment and 8 chicks per replicate, and fed with their respective diet during the starter (8 to 21 days) and grower (22 to 42 days) periods. Diets were a basal corn-soybean based diet (T1), or diets containing 20 % rice bran (T2), 30 % rice bran (T3), 20 % extruded rice bran (T4), or 30 % extruded rice bran (T5). Results: Birds feeding on T4 and T5 diets had a higher body weight gain and lower feed-to-gain ratio compared to those feeding on T2 and T3 diets (p < 0.05). Birds receiving diets containing extruded rice bran had higher total P availability and tibia ash content, as compared with those receiving diets containing un-extruded rice bran (p < 0.05). Relative weight of the pancreas was higher in birds receiving T2 and T3 diets. Conclusions: The results confirmed the beneficial effect of extrusion treatment of rice bran on performance and P availability in broilers. Up to 30 % extruded rice bran may be included in the broiler diet without apparent adverse effects on the performance.

Comparative Evaluation of Resolution according to Frequency Change for SPECT (단일광자방출전산화단층촬영(SPECT)을 위한 주파수 변동에 따른 분해능 비교평가)

  • Kim, Sung-Chul;Park, Soo-Yeun;Cho, Young-Kwon;Ahn, Sung-Min
    • The Journal of the Korea Contents Association
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    • v.9 no.12
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    • pp.322-331
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    • 2009
  • In clinical SPECT, FWHM provides quality information of the images obtained under different observing conditions. Therefore, the purpose of this study was to determine the optimum cut off level with comparison of FWHM according to cut off levels in each filter - Band limited, Sheep-Logan, Hanning, Hamming, Low pass cosine, Parazen and Butterworth filter in a SPECT camera. I recorded images along the X, Y, Z-axis with 99mTcO4 point source and measured FWHM with profile curves. In conclusion, all filters showed the longest figures of FWHM with cut off level 0.4, which has the worst image resolution. The images with cut off level 0.7 showed best image resolution. The shortest average of FWHM in MS2 was $11.07\pm0.07mm$ using the Butterworth filter, in MS3 it was $8.44\pm0.19mm$ through using the Hanning filter.

Effects of Replacing Soybean Meal with Fermented Rapeseed Meal on Performance, Serum Biochemical Variables and Intestinal Morphology of Broilers

  • Xu, F.Z.;Zeng, X.G.;Ding, X.L.
    • Asian-Australasian Journal of Animal Sciences
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    • v.25 no.12
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    • pp.1734-1741
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    • 2012
  • This trial was performed to study the effects of replacing soybean meal (SBM) with fermented rapeseed meal (RSM) on growth performance, serum biochemistry variable and intestinal morphology of broilers. A total of 640 d-old Arbor Acres broiler chicks were randomly allocated to 4 dietary treatments, 4 pens per treatment and 40 birds per pen for a 6-wk feeding trial. In the four treatment groups, fermented RSM replaced soybean meal at 0, 5, 10, and 15%, respectively. On 21 d and 42 d, two birds from each pen were randomly selected and slaughtered. Blood samples and sections of duodenum, jejunum, and ileum were collected for measurement of serum biochemical variables and intestinal morphology, respectively. Results showed that body weight gain (BWG) and feed conversion (FC) were significantly (p<0.01) poorer for birds fed the 15% fermented RSM diet than those fed with 0, 5 and 10% fermented RSM diets during all periods. Compared with 0 and 5% fermented RSM groups, IgG content in the serum of birds in 10 and 15% fermented RSM groups was improved (p<0.01) urea nitrogen content of serum was reduced (p<0.01) during both growing and finishing periods. However, IgM, phosphorus and calcium levels increased (p<0.05) only during the growing period. Increased (p<0.05) villus height was observed in the duodenum and jejunum of broilers fed the diet with 10% fermented RSM. In addition, villus height to crypt depth ratio in the jejunum was significantly higher (p<0.01) for birds fed the diet with 10% fermented RSM than for those fed diets with 0, 5 and 15% fermented RSM. The present results suggest that RSM fermented with Lactobacillus fermentum and Bacillus subtilis is a promising alternative protein source and that it could be safely used replace up to 10% SBM in broiler diets.

Power Generation Characteristics of Uni-morph Piezoelectric Cantilever with Different Vibration Angle (진동 각도에 따른 유니몰프 압전 캔틸레버의 발전특성연구)

  • Kim, Chang Il;Yun, Ji Sun;Park, Woon-Ik;Jeong, Young-Hun;Hong, Youn Woo;Cho, Jeong-Ho;Paik, Jong Hoo
    • Journal of Sensor Science and Technology
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    • v.26 no.2
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    • pp.107-113
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    • 2017
  • Energy source of a piezo-electric harvester is vibration. Sources of vibration are machineries operated with high frequencies, constructions and people operated with low frequencies and etc. In this study, we tried to figure out power generation properties over vibrations upon angles of a piezo-cantilever for applying them to movements of the construction and/or people, which are vibration sources at low frequencies. A uni-morph cantilever with a $59mm{\times}29mm{\times}0.2mm$ piezo-electric element attached on a $71mm{\times}46mm{\times}0.25mm$ copperplate was used. A spring was attached to the lower side of the cantilever and a mass was attached on the opposite side. Also, a structure with a specific angle which is an angle in between the ground and the cantilever was prepared and then, connected to a spring or the cantilever. Then, this structure was divided into the A-type and B-type and excited in the direction of z- axis. After that, the angle between the ground and the cantilever was changed and excited by 1 to 10 Hz upon the existence of a spring and/or a mass to compare power generation properties.

A Study on the Dry Matter Yield and Qualityof Festulolium braunii at Different Growing Stages (생육단계별 Festulolium braunii의 건물수량 및 사료가치 구명)

  • 이인덕;이형석
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.17 no.2
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    • pp.117-122
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    • 1997
  • This experiment was conducted to evaluate the forage yield and quality and the possibility to provide Festulolium bmunii(Festuca pratensis $Huds{\times}Lolium$ mult~jlotum Lam.) as forage source in Korea. The Festuloliurn braunii were harvested at boot, heading and anthesis stage, respectively. The field trials were performed 6om 1994 to 1996 at the forage experimental field, College of agriculture, Chunpam National University. The results obtained were summarized as follows: 1. The date of boot, heading and anthesis stage of Festulolium bmunii observed in Taejon were May 12, May 23 and June 1 in 1995, and June 1, June 4 and June 9 in 1996, respectively. Winter survival percentage of Festulolium bmunii war ranged 6om 96% to 98% and summer survival percentage were 75-86%. It would suggest that Festulolium brawlii persisted well in the aspect of overwintering, while it wasn' t widely adapted to drought and high temperature in Taejon regions. 2. The CP content and DMD of Festulolium bmunii tended to decline as the growth stage advanced. The CP content and DMD of Festulolium bmunii were high at the boot stage and those at the anthesis stage were low. But fiber contents at the boot stage were lower than those at anthesis stage(P< 0.05). The yields of the DM and DDM with advancing the growing stage tended to increase, while there was no difference in the CPDM yields among stages. On the otherhand, the yields of DM and DDM at the anthesis stage were the highest (P< 0.05), but there was no difference in CPDM yield among stages. Based on the results mentioned above, it is suggested that Festulolium b m n i i has a possibility to use until the anthesis stage under the cutting regimes and that it has a potentiality to provide one of a good forage sources. %'ifqqiZ 43q *(College of Agriculture, Chungnam National Un~versity, Taejon 305-764, Korea)

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