• Title/Summary/Keyword: Young Pigs

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Isolation and Identification of Porcine Rotavirus from Piglets with Diarrhoea (하리자돈(下痢子豚) 분변(糞便)에서 Rotavirus 분리(分離) 동정(同定))

  • Kim, Hee-sun;Choi, Chung-ok;Kim, Doo-hee;Lyoo, Young-soo;An, Soo-hwan
    • Korean Journal of Veterinary Research
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    • v.28 no.1
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    • pp.89-97
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    • 1988
  • Twenty-eight porcine rotavirus were isolated from piglets with diarrhoea in chonnam province. According to the age, 41 to 60 day old pigs showed the highest isolation frequency among the post weaning pigs. The characteristics of the field isolates were determined by electronmicroscopy(EM), immunofluorescent assay(FA), and electrophoretic migration patterns of the genome profiles. Some of the isolates showed remarkable haemagglutination activity against rabbit and dog erythrocytes, ranged from 4 to 2848, respectively. At least 3 serotypes of porcine rotavirus were recognized by serum neutralization test using serotype specific rotavirus hyperimmune sera.

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Protective Effect of Diallyl disulfide(DADS) from Garlic Extract on Noise Induced Hearing Loss in the Guinea pigs (소음으로 인한 청력소실에 대한 마늘 추출물 diallyl disulfide(DADS)의 효과)

  • Yoon, Min-Young;Cho, Nam-Geun;Hwang, Woo-Jun;Jang, Chul-Ho
    • Journal of Acupuncture Research
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    • v.19 no.2
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    • pp.105-113
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    • 2002
  • Objective : The purpose of this study is to investigate the protective effect of DADS on the noise induced hearing loss Methods : Twenty healthy guinea pigs (DADS pretreated group and control) were exposed to 100dB a collected environmental noise for 9 hours. An auditory evoked brainstem response (ABR) threshold was measured before and after a noise exposure. For morphological study, cochlea was studied by scanning electron microscope (SEM). Results : The DADS pretreated group showed statistically significant less changes in ABR threshold and SEM findings compared the control group. Conclusion : From these results suggest that the DADS from garlic may have a protective effect in noise induced hearing loss.

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Postweaning Multisystemic Wasting Syndrome - Case studies

  • Kim, Jae-Hoon;Roh, In-Soon;Hwang, Eui-Kyung;Woo, Gyu-Hywong;Jean, Young-Hwa;Sohn, Hyun-Joo;Yoon, Kyoung-Jin;Janke, Bruce H.;Park, Choi-Kyu;Kim, Ki-Seuk
    • Proceedings of the Korean Society of Veterinary Pathology Conference
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    • 2000.09a
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    • pp.28-28
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    • 2000
  • Postweaning Multisystemic Wasting Syndrome (PMWS) is a recently described disease of growing pigs characterized by progressive weight loss and chronic pneumonia. PMWS was first identified in western Canada in 1995, and has now been diagnosed in the US, Europe and Asia. Since 1999, 57 pigs from 23 farms of PMWS have been diagnosed in the National Veterinary Research and Quarantine Service (NVRQS). The most common age of onest was clustered around 6-week age. (omitted)

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Chemically-Induced Delayed Cutaneous Hypersensitivity in Guinea Pigs and Rabbits Experimentally Infected with Mycobacterium bovis (Mycobacterium bovis 감염(感染)이 화학적(化學的)으로 유발(誘發)된 지연형(遲延型) 피부과민증(皮膚過敏症)에 미치는 영향(影響))

  • Lee, Chai-yong;Lee, Chung-gil;Park, Young-jun
    • Korean Journal of Veterinary Research
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    • v.26 no.2
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    • pp.293-299
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    • 1986
  • In order to evaluate the effects of the cell-mediated immunity of the animal in the chronic diseases the guinea pigs and rabbits were inoculated with Mycobacterium bovis. After 6 weeks these animals were sensitized and challenged with 2,4-dinitrochlorobenzene. The cutaneous reactions observed in these animal species were similar each other. Macroscopic and microscopic responses in the animal experimentally infected with M. bovis were markedly reduced compared to those in the control animals. The results indicated that the cell-mediated immunity of the animals was depressed by infection of M. bovis.

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Cross-Reactivity of Porcine Immunoglobulin A Antibodies with Fecal Immunoglobulins of Wild Boar (Sus scrofa) and Other Animal Species

  • Sang won Seo;Sung J. Yoo;Sunyoung Sunwoo;Bang hun Hyun;Young S. Lyoo
    • IMMUNE NETWORK
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    • v.16 no.3
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    • pp.195-199
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    • 2016
  • Fecal samples obtained from wild boar habitats are useful for the surveillance of diseases in wild boar populations; however, it is difficult to determine the species of origin of feces collected in natural habitats. In this study, a fecal IgA ELISA was evaluated as a method for identifying the porcine species from fecal samples. Both domestic pigs (Sus scrofa domestica) and wild boars (Sus scrofa coreanus) showed significantly higher levels of fecal IgA than other animal species. Additionally, age dependent changes in the level of Ig A in wild boars and domestic pigs were identified; Titers of Ig A were highest in suckling period and lowest in weanling period.

Effect of Zizyphus vulgaris Supplementation on Growth Performance, Blood Cortisol and Meat Quality Characteristics in Finishing Pig (비육돈 사료내 산조인(Zizyphus vulgaris)의 급여가 성장 혈액내 Cortisol 및 육질 특성에 미치는 영향)

  • Cho Jin-Ho;Han Young-Geun;Kwon Oh-Suk;Min Byoung-Joon;Son Kyoung-Seung;Chen Ying-Jie;Kim In-Ho
    • Food Science of Animal Resources
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    • v.25 no.1
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    • pp.20-25
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    • 2005
  • This study was conducted to evaluate the effects of Zizyphus vulgaris supplementation on growth performance, blood cortisol and meat quality characteristics in finishing pigs. The total of thirty-six [Duroc${\times}$Yorkshir${\times}$Landrace] pigs ($91{\pm}2.11$ kg average initial body weight) were used in a 30-days assay. Dietary treatments included 1) CON (basal diet), 2) T1 (basal diet for 15 days and 0.1 % Zizyphus vulgaris for 15 days) and 3) T2 (0.1 % Zizyphus vulgaris for 30 days). The ADG (Average daily gain), ADFI (Average daily feed intake) and ADG/ADFI during the feeding period were not significantly differences among the treatments (p>0.05). Backfat thickness of pigs fed CON was higher than those of T1 and T2 (p<0.05). The appearance rate of A or B carcass grade was in T1 (74%) and T2 (84%) was significantly higher than that in CON (58%) (p<0.05). Pigs fed Zizyphus vulgaris 0.1 % for 30 days tended to decrease on blood cortisol compared with pigs fed CON and T1. But, there was not significantly difference among the treatments (p>0.05). The Hunter's L/sup */ (lightness) value of loin in the pork fed CON was higher than that of loin in the pork fed T1 and T2 (p<0.05). After 7 days, the L/sup */ value of loin in the pigs fed T2 was higher increased than that of pigs fed T1 and CON (p<0.05). However, a/sup */ and b/sup */ values were not affected by dietary Zizyphus vulgaris (p>0.05). There were not found remarkable differences in sensory properties (marbling, firmness and color) among the treatments. The results from the present study suggest that Zizyphus vulgaris could be a effective feed additive to improve meat quality of pigs. However, further research is needed to investigate effects of carcass characteristics.

Agricultural and Marine By-Products Fermented Diet and Its Economic Value in Pigs (농수부산물 발효사료가 양돈에 미치는 경제적 효과 분석)

  • Chu, Gyo-Moon;Kim, Hoi-Yun;Ha, Ji-Hee;Yang, Jeong-Mo;Yang, Bo-Seock;Park, Cheol-Jin;Song, Young-Min
    • Journal of agriculture & life science
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    • v.46 no.3
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    • pp.59-68
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    • 2012
  • This study was conducted to investigate the economic analysis of fermented diet made of agricultural and marine by-products such as, dropped apples, persimmon shells, mushroom (Flammulina velutipes) by-products, king oyster mushroom by-products and brewer's grain in Berkshire or crossed pigs. The daily diet cost were decreased by 2.16~3.44%, 3.83~13.35% and 7.47~41.61% when fermented dropped apples diet, fermented persimmon shells diet and fermented mushroom (F. velutipes) by-products diet were fed to Berkshire pigs. The daily feed cost was also decreased by 1.59~4.05% and 59.09~66.90% when fermented king oyster mushroom by-products diet and fermented brewer's grain diet were fed to crossed pigs. Carcass prices were increased by 3.09%, 1.20~3.40% and 1.13% when 2% fermented dropped apples diet, fermented persimmon shells diet and 0.5~1.0% fermented mushroom (F. velutipes) by-products diet were fed to Berkshire pigs. Carcass prices were increased by 2.87~4.19% and 41.31~49.14% when fermented king oyster mushroom by-products diet and fermented brewer's grain were fed to crossed pigs. Therefore, fermented agro by-products diet of dropped apples, persimmon shells, king oyster mushroom, mushroom (F. velutipes) and brewer's grain was expected to improve swine farms income due to decreased diet cost and increased carcass prices in Berkshire pigs or crossed pigs.

Alpha-1,3-galactosyltransferase-deficient miniature pigs produced by serial cloning using neonatal skin fibroblasts with loss of heterozygosity

  • Kim, Young June;Ahn, Kwang Sung;Kim, Minjeong;Kim, Min Ju;Ahn, Jin Seop;Ryu, Junghyun;Heo, Soon Young;Park, Sang-Min;Kang, Jee Hyun;Choi, You Jung;Shim, Hosup
    • Asian-Australasian Journal of Animal Sciences
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    • v.30 no.3
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    • pp.439-445
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    • 2017
  • Objective: Production of alpha-1,3-galactosyltransferase (${\alpha}GT$)-deficient pigs is essential to overcome xenograft rejection in pig-to-human xenotransplantation. However, the production of such pigs requires a great deal of cost, time, and labor. Heterozygous ${\alpha}GT$ knockout pigs should be bred at least for two generations to ultimately obtain homozygote progenies. The present study was conducted to produce ${\alpha}GT$-deficient miniature pigs in much reduced time using mitotic recombination in neonatal ear skin fibroblasts. Methods: Miniature pig fibroblasts were transfected with ${\alpha}GT$ gene-targeting vector. Resulting gene-targeted fibroblasts were used for nuclear transfer (NT) to produce heterozygous ${\alpha}GT$ gene-targeted piglets. Fibroblasts isolated from ear skin biopsies of these piglets were cultured for 6 to 8 passages to induce loss of heterozygosity (LOH) and treated with biotin-conjugated IB4 that binds to galactose-${\alpha}$-1,3-galactose, an epitope produced by ${\alpha}GT$. Using magnetic activated cell sorting, cells with monoallelic disruption of ${\alpha}GT$ were removed. Remaining cells with LOH carrying biallelic disruption of ${\alpha}GT$ were used for the second round NT to produce homozygous ${\alpha}GT$ gene-targeted piglets. Results: Monoallelic mutation of ${\alpha}GT$ gene was confirmed by polymerase chain reaction in fibroblasts. Using these cells as nuclear donors, three heterozygous ${\alpha}GT$ gene-targeted piglets were produced by NT. Fibroblasts were collected from ear skin biopsies of these piglets, and homozygosity was induced by LOH. The second round NT using these fibroblasts resulted in production of three homozygous ${\alpha}GT$ knockout piglets. Conclusion: The present study demonstrates that the time required for the production of ${\alpha}GT$-deficient miniature pigs could be reduced significantly by postnatal skin biopsies and subsequent selection of mitotic recombinants. Such procedure may be beneficial for the production of homozygote knockout animals, especially in species, such as pigs, that require a substantial length of time for breeding.

Effects of a lipid-encapsulated zinc oxide supplement on growth performance and intestinal morphology and digestive enzyme activities in weanling pigs

  • Jang, Insurk;Kwon, Chang Hoon;Ha, Duck Min;Jung, Dae Yun;Kang, Sun Young;Park, Man Jong;Han, Jeong Hee;Park, Byung-Chul;Lee, Chul Young
    • Journal of Animal Science and Technology
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    • v.56 no.8
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    • pp.29.1-29.6
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    • 2014
  • This study compared the effects of varying lipid content and dietary concentration of a lipid-encapsulated (LE) ZnO product to those of native ZnO and thereby to find insights into optimal lipid coating and dosage of the Zn supplement. A total of 192 21-d-old weanling pigs were allotted to 48 pens, after which each six pens received a ZnO-free basal diet supplemented with 125 ppm ZnO (100 ppm Zn; BASAL), 2,500 ppm Zn as native ZnO (HIGH), or 100 or 200 ppm Zn as LE ZnO (LE-100 or LE-250) containing 8%, 10%, or 12% lipid [LE-8%, LE-10%, or LE-12%, respectively; $2{\times}3$ factorial arrangement within the LE-ZnO diets (LE-ALL)] for 14 d. Forty pigs were killed at the end for histological and biochemical examinations. None of ADG, ADFI, gain:feed, and fecal consistency score differed between the LE-ALL and either of the BASAL and HIGH groups. Hepatic and serum Zn concentrations were greater (p <0.05) in the HIGH vs. LE-ALL group, but did not differ between LE-ALL and BASAL, between LE-100 and -250, or among LE-8%, -10%, and -12% groups. Villus height (VH), crypt depth (CD), and the VH:CD ratio in the duodenum, jejunum, and ileum did not differ between the LE-ALL and either of the BASAL and HIGH groups, except for a greater CD in the duodenum in the LE-ALL vs. HIGH group. Additionally, VH and CD in the duodenum and VH:CD in the jejunum were greater in the LE-250 vs. LE-100 group. Specific activities of sucrase, maltase, and leucine aminopeptidase in these intestinal regions and those of amylase and trypsin in the pancreas were not influenced by the lipid content or dietary concentration of LE ZnO and also did not differ between the LE-ALL and either of the BASAL and HIGH groups, except for a greater pancreatic amylase activity in the former vs. HIGH group. In conclusion, the present results indicate that the LE ZnO, regardless of its lipid percentage or supplementation level examined in this study, has no significant effect on growth performance, fecal consistency, or digestive enzyme activities of weanling pigs under the experimental conditions.