• Asian-Australasian Journal of Animal Sciences
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• v.22 no.8
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• pp.1069-1077
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• 2009

Castration of male pig produces significant negative effects on skeletal muscle development. The androgen receptor (AR), two splice variants of insulin-like growth factor-I (IGF-I Ea and MGF) and the myostatin gene may play important roles in this process. In the present study, the expression of AR, IGF-I Ea, MGF and myostatin genes in three skeletal muscles, the brachialis, longissimus and semitendinosus, were studied using real-time quantitative RT-PCR. Our experimental design used 14 pairs of male Landrace sire${\times}$Yorkshire dam piglets. The two piglets in each pair were full sibs, one of which was castrated at 21 d of age; the other remained intact. The study group was divided into subgroups of equal size. Animals in the first subgroup were slaughtered at 147 d and those of the second at 210 d of age. Carcass weight and lean meat yield were similar between boars and barrows at 147 d of age (p>0.05), whereas barrows had lower carcass weight and less lean meat yield at 210 d of age (p<0.05). Castration caused down-regulation of AR gene expression at both 147 and 210 d of age (p<0.05). The two splice variants of the IGF-I gene from porcine skeletal muscle were cloned using RT-PCR, and it was found that MGF differs from IGF-I Ea in having a 52-base insert in the last coding exon of the mRNA. Both splice variants were down-regulated by castration only at 210 d of age (p<0.05). No differences in expression of the myostatin gene were observed between boars and barrows at either 147 or 210 d of age (p>0.05). These results suggest that the downregulation of AR, IGF-I Ea and MGF gene expression following castration helps to explain the negative effect of castration on skeletal muscle development.

• Korean Journal of Agricultural Science
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• v.3 no.2
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• pp.192-197
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• 1976

Study was conducted to estimate the nutritional effect of substitution levels of "IKUTON" (R) inocculate fermented sweet potato pulp for the growing-finnishing pig rations. Twenty five Large white X Landrace cross-breed (15 kg). mixed sex were used in group feeding at the substitution levels of 0, 10, 20, 30, and 40%. The results obtained were summarized as follow: Voluntary feed intake of experimental diets was lowest in 40%. No significant difference in voluntary feed intake was found in 10,20, and 30% levels except 40% P<0.05). The feed efficiency was highest in 20%; (3.43), 30%; (3.80), 10%; (3.97), control; (4.53), and 40%; (4.20) respectively. There was tendency toward increasing feed effioiency in 10,20,30% levels than control. However, the lowest feed efficiency was found in 40% level. The weeks required to gain 80 kg of live weight were 12 weeks for 10%, 13 weeks for control. 14 weeks for 20%, and 30%, but in 40% level of substitution, no 80kg live weight was found up to the end of experiment (15 weeks). Pigs in 40% level were gained live weight only 64% of control animals. The digestibility of experimental diets was 75.2-78.5%, and water intake per kg dry matter intake was $1.95-2.01{\ell}$. No significant effects of feed offer interval to the digestibility and water consumption.

• Korean Journal of Agricultural Science
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• v.42 no.4
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• pp.375-382
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• 2015

In the present study, offsprings of 14 sows were used to evaluate the effect of fermented milk substituted for creep feed to analyze its effect on growth performance, diarrhea score and blood profiles. The piglets of sows were randomly allocated into 1 of 2 treatments (1 treatment separated piglets of 7 sows). The dietary treatments includes: CF (creep feed) and FM (fermented milk). The CP and FM diets were given from 3 d after piglets were born to the day of weaning (21 d after piglets were born). No significant difference (P>0.05) were observed in number of born piglets, weaning pigs, survival rate, body weight of piglets at d 0, 7, 14, and 21, ADG and ADFI. At d 21, piglets fed with CP had a higher (P<0.05) IgG compared with piglets fed with FM. No significant difference (P>0.05) was observed in WBC among the treatments at d 21. At d 6, piglets fed with CP had a lower (P<0.05) number of diarrhea piglets compared to piglets fed with FM. In conclusion, FM substituted for CP had no adverse effect on growth performance in lactating piglets.

• Journal of Veterinary Clinics
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• v.9 no.2
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• pp.407-416
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• 1992

This experiment was conducted to investigate the feed value of dried aloe leaf meal and the changes of blood values in growing pigs A total of 48 three crossbred pig(Landrace${\times}$Yorkshire${\times}$Hampshire) weighing average 25kg initially were randomly distributed into 12 groups of 4 heads(2 females and 2 males) each There groups were alloted on one of the following 3 dietary treatment : non-supplumented diet (control group), diet supplemented with 3% of dried aloe leaf meal(Aloe 3% group), diet supplemented with 6% dried aloe leaf meal(Aloe 6% group). The results obstained in feeding trial for 6 weeks and analysis of blood were summarized as follows : 1. In the chemical composition of dried aloe leaf meal contents of crude protein and crude ash were 9.43% and 15.10%, respectively. Amino acid composition was also inferior to other grain and bran feeds. 2. Daily gain of control, Aloe 3% and 6% groups were 740.5, 658.1 and 197.1 g respectively. Three were significant difference in daily gain among groups( p<0.05) and tended to be decreased with increasing levels of dried aloe leaf meal. 3. Daily feed intake of control, Aloe 3% and Aloe 6% groups were 1,960.5, 1,737.0 and 1,123.0 g, respectively. There were significant differences in daily feed intake among treatments(p<0.05) and tended to be decreased with increasing levels of dried aloe leaf meal. 4. Feed efficiency of control, Aloe 3% and Aloe 6% groups were 2.72, 2.63 and 5.70 respectively. Feed efficiency for pig fed diet supplemented with 3% of dried aloe leaf meal was a little superior to control, although no statistical difference was obstained between two treatments. 5. The digestibilities of dry matter, crude protein, nitrogen free extract and extract were significantly (p<0.05) higher for control group than for Aloe 3% and Aloe 6% groups. But there were no significant differences in digestibility of crude fat and crude fiber between control and Aloe 3% groups. 6. There were no difference between aloe leaf meal administrated groups and control group in blood picture and serum chemistry.

• Food Science of Animal Resources
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• v.27 no.2
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• pp.150-156
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• 2007

This study was conducted to analyze the genetic characteristics of Korean Native Pigs(KNP), and to establish an individual identification system comprising many microsatellite markers located on different pig autosomes. Genotype data from 13 microsatellites typed in 446 animals was used to determine the validation of a method of individual identification in 4 KNP. A total of 112 alleles of the 13 microsatellites were detected and average heterozygosities(polymorphic information content) ranged from 0.286(0.423) to 0.686(0.796) in this study. Comparing the pattern of allele frequency among the KNP, Yorkshire, Landrace and Duroc breeds, there was specific differentiation between populations at multi-allelic loci. The cumulative power of discrimination(CPD) was 99.999% by including 10 microsatellite loci for the individual identification system. The probability that two different individuals incidentally have same genotype was estimated to be $0.36{\times}10^{-9}$. The system employing these 10 markers therefore proved to be applicable to the individual identification of KNP.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.7
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• pp.974-979
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• 2000

A total of 80 weaned piglets (Landrace $\times$ Yorkshire $\times$ Large White) were used in a 28-day growth assay to detennine the optimal inclusion level of sodium sulfite ($Na_{2}SO_{3}$) as an extrusion enhancer of soybean meal for nursery piglets. piglets (21 d of age, 6.04 kg of BW) were grouped into 4 treatments in a completely randomized block design. Treatments were: 1) Extruded SBM (Control), 2) Extruded SBM with 0.5% $Na_{2}SO_{3}$ (0.5 ESBM), 3) Extruded SBM with 1.0% $Na_{2}SO_{3}$ (1.0 ESBM) and 4) Extruded SBM with 1.5% $Na_{2}SO_{3}$ (1.5 ESBM). Each treatment has 4 replicates of 5 heads per pen. In phase I (d 0 to 14), diets supplied 3,400 kcal ME/kg, 23% crude protein, 1.65% lysine, 0.50% methionine, 0.9% Ca and 0.8% P. Phase II (d 14 to 28) diets contained 3,300 kcal ME/kg, 21% crude protein, 1.45% lysine, 0.45% methionine, 0.9% Ca and 0.8% P. For d 0 to 14, piglets fed 1.5 ESBM had greater ADG, ADFI and FCR compared to piglets fed control and 0.5 ESBM diet. ADG was significantly higher in piglets fed 1.5 ESBM diet than other groups (p<0.05) except 1.0 ESBM. In phase II (d 14 to 28), there was no significant differences in production traits among treatments. For overall period (d 0 to 28), piglets fed diets with high sodium sulfite grew faster than piglets fed control and 0.5 ESBM diets. The highest ADG and the best FeR were obtained in piglets fed diets with 1.5 ESBM during the entire period. Piglets fed 1.5 ESBM diet showed significantly higher crude protein digestibility than 0.5 ESBM (p<0.05) at d 14 post-weanling, but not at d 28 post-weanling. There were no significant differences in digestibilities of total amino acids. In conclusion, the addition level of 1~1.5% sodium sulfite for SBM extrusion could be favorable for rate and efficiency of growth in weaning pigs.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.3
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• pp.324-328
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• 2006

Carbonic anhydrase III (CA3) is a member of a multigene family that encode carbonic anhydrase isozymes. In this study, a complete coding sequence of the pig CA3 gene which encodes a 260 amino-acid protein was determined. The amino acid comparison showed high sequence similarities with previously identified human (86.5%) CA3 gene and mouse (91.5%) Car3 gene. The partial genomic DNA sequences were also investigated. The length of intron 1 was 727 bp. Comparative sequencing of three pig breeds revealed that there was a T${\rightarrow}$C substitution at position 363 within intron 1. The substitution was situated within a NcoI recognition site and was developed as a PCR-restriction fragment length polymorphism (RFLP) marker for further use in population variation investigations and association analysis. Two alleles (A and B) were identified, and 617 bp fragments were observed for the AA genotype and 236 bp and 381 bp fragments for the BB genotype. The polymorphism of CA3 was detected in 8 pig breeds. Allele B was predominant in the Western pig breeds. In addition, association studies of the CA3 polymorphism with carcass traits in 140 $Yorkshire{\times}Meishan$ $F_2$ offspring showed that the NcoI PCR- RFLP genotype may be associated with variation in several carcass traits of interest for pig breeding. Allele B was associated with increases in lean meat percentage, loin eye height and loin eye area. Statistically significant association with backfat thickness was also found; pigs with the AB genotype had much less backfat thickness than AA or BB genotypes.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.6
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• pp.862-869
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• 2015

The current study was designed to estimate the pork quality traits using metabolites from exsanguination blood and postmortem muscle simultaneously under the Korean standard pre- and post-slaughter conditions. A total of 111 Yorkshire (pure breed and castrated male) pigs were evaluated under the Korean standard conditions. Measurements were taken of the levels of blood glucose and lactate at exsanguination, and muscle glycogen and lactate content at 45 min and 24 h postmortem. Certain pork quality traits were also evaluated. Correlation analysis and multiple regression analysis including stepwise regression were performed. Exsanguination blood glucose and lactate levels were positively correlated with each other, negatively related to postmortem muscle glycogen content and positively associated with postmortem muscle lactate content. A rapid and extended postmortem glycolysis was associated with high levels of blood glucose and lactate, with high muscle lactate content, and with low muscle glycogen content during postmortem. In addition, these were also correlated with paler meat color and reduced water holding capacity. The results of multiple regression analyses also showed that metabolites in exsanguination blood and postmortem muscle explained variations in pork quality traits. Especially, levels of blood glucose and lactate and content of muscle glycogen at early postmortem were significantly associated with an elevated early glycolytic rate. Furthermore, muscle lactate content at 24 h postmortem alone accounted for a considerable portion of the variation in pork quality traits. Based on these results, the current study confirmed that the main factor influencing pork quality traits is the ultimate lactate content in muscle via postmortem glycolysis, and that levels of blood glucose and lactate at exsanguination and contents of muscle glycogen and lactate at postmortem can explain a large portion of the variation in pork quality even under the standard slaughter conditions.

• Journal of Animal Science and Technology
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• v.51 no.4
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• pp.283-288
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• 2009

This study was carried out to compare Msp I polymorphisms in the 3rd intron of porcine gene encoding the pituitary-1 transcription factor (POU1F1) from 286 pigs (Landrace $\times$ Yorkshire $\times$ Duroc, LYD) and to determine the associations between its genotypes and carcass traits by using the PCR-RFLP technique. The frequency of the single nucleotide polymorphism (SNP) genotype DD (84.33%) was very higher than that of CC genotype (0.75%). Allelic frequencies for C and D were 0.082 and 0.918, respectively. Each population followed the Hardy-Weinberg equilibrium. Meat colours of Hunter $L^*$ values and visual colour according to two genotypes were all significantly different. However, no significant difference in crossbred (LYD) was found between CD and DD genotypes for other traits. Therefore, this suggests that POU1F1 may be a major gene or marker for carcass traits.

• Journal of Animal Science and Technology
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• v.52 no.3
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• pp.187-190
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• 2010

The objective of this study was to estimate genetic parameters for total number of born (TNB), number of born alive (NBA) and teat numbers (TN) of Landrace and Yorkshire breeds in Korean swine population using multiple trait animal model procedures. Total numbers of 4,653 records for teat numbers and 8,907 records for TNB and NBA collected from 2004 to 2008 on imported breeding pigs and their litter size records were used in this study. To find the appropriate model for estimation of genetic parameters (heritabilities and genetic correlations), five statistical models (two models for reproductive traits, two models for teat numbers, one model for combining these traits) considering only direct additive genetic effects, including maternal effects were used and Akaike information criteria (AIC) of each two models for reproductive traits and teat trait were compared. The means and standard deviations of TNB, NBA, and TN were $11.52{\pm}3.34$, $10.55{\pm}2.96$ and $14.30{\pm}0.83$, respectively. Estimated heritabilities for TNB and NBA traits using the model which considered only additive genetic effect were low (0.06 and 0.05, respectively). However, estimated heritabilities considering maternal genetic effects were a little bit higher than that of the model considering only additive genetic effect (0.09 for TNB and NBA, respectively). Estimated heritability for TN using the model which considered only additive genetic effect was 0.40. However, estimated heritability of direct genetic effects from a model considering maternal genetic effect was high (0.60). All results of AIC statistics, the models considering maternal effect was more appropriate than the models considering only additive genetic effect. Genetic correlations of direct additive genetic effect between litter size (TNB, NBA) and teat numbers were low (-0.18 and -0.14, respectively). However, genetic correlations of maternal effect between litter size (TNB, NBA) and teat numbers were a little bit higher than those of direct additive genetic effect (0.08 and 0.16, respectively).