• Journal of Life Science
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• v.8 no.3
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• pp.263-271
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• 1998

One of the better-characterized transcription factor of E. coli is the cAMP receptor protein(CRP) and the CRP binds cAMP and DNA. The cAMP-CRP complex is involved in regulation of many genes at bacteria. The cAMP-CRP regulatory element represents, in some respects, a global regulatory network. The aim of this work was to study the structure and the mechanisms controlling the expression of CRP in Serratia marcescens. We have been get 5 different clones from Serratia which stimulated the cells to use maltose as a sole carbon source in E. coli TP2139. The crp gene clone, pCKB12, was confirmed by Southern hybridization with E. coli crp gene. The location of the crp gene was determined by construction subclones carrying various portions of pCKB12. To investigate the potential role of CRP in E. coli, lacZ fused plasmids were constructed and investigated the ${\beta}$-galactosidase activity of the fused plasmid. The Serratiamarcescens cAMP receptor protein can substitute the E. coli CRP in transcriptional activation at the lacZ gene. These results suggest that Serratia marcescens cAMP receptor protein complex functions to regulate several promoters in E. coli.

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.6
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• pp.912-917
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• 2011

The sterilization efficacies of various washing solutions on the surfaces of vegetables such as sesame leaves, lettuce, and mini-cabbage were investigated. The washing solutions were tap water (TW), microbubble water (MB), electrolyzed water (EW), and microbubble electrolyzed water (MB＋EW). After Escherichia coli and Bacillus cereus were artificially inoculated onto the surfaces of vegetables, each vegetable was washed for 1, 3, and 5 min with TW, MB, EW 100 (100 mg/L of available chlorine), EW 200 (200 mg/L of available chlorine), MB+EW 100, and MB＋EW 200. The washing efficacy of MB was slightly higher than that of TW, and EW was more effective than MB (p<0.05). In all instances, the sterilization efficacies of MB+EW 100 and MB+EW 200 were higher than those of EW 100 and EW 200 (p<0.05). Thus, MB+EW offers an effective means of reducing the studied microorganisms in a short time period. The MB＋EW washing method provides microbial reduction on the surfaces of various vegetables and enhances the microbiological safety of the vegetables.

• The Journal of Internal Korean Medicine
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• v.26 no.3
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• pp.551-562
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• 2005

Objectives : The aim of this study was to investigate the effect of Trichosanthis Semen Herbal-acupuncture(TS-HA) at Joksamni(ST36) on ovalbumin-induced asthma in mice. Methods : C57BL/6 mice were sensitized and challenged with ovalbumin(OVA) once a week for twelve weeks. The experimental group was treated with 1% concentrations of TS-HA at Joksamni(ST36) three times a week for the last eight weeks. Results : 1. The weight and total lung cells of the mice in the group treated with TS-HA decreased significantly compared with those of control group. 2. Total leukocytes and eosinophils in Bronchoalveoler-lavage fluid(BALF) of the mice in the group treated with TS-HA decreased significantly compared with those of control group. 3. Eosinophils in BALF of the mice in the group treated with TS-HA in photomicrographs decreased significantly compared with those of control group. 4. According to histological analysis of lung sections, adhension of collagen in TS-HA decreased significantly compared with that of control group. 5. The concentration of IgE, IL-4, IL-5 in BALF and IL-4, IL-5, IL-13 in serum of the mice in the group treated with TS-HA decreased significantly compared with that of control group. 6. The number of $Gr-^+/CD11b^+,\;CD11b^+,\;CD3e^-/CCR3^+,\;CD4^+,\;CD8^+\;CD3e^+/CD69^+,\;CD23^+/B220^+\;cells$ in the lungs of the mice in the group treated with TS-HA decreased significantly compared with those of control group. Conclusion : These results suggest that Trichosanthis Semen-herbal acupuncture at Joksamni(ST36) done on C57BL/6 mice is effective in part in relieving OVA-induced asthma in C57BL/6 mice.

• Journal of Adhesion and Interface
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• v.18 no.3
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• pp.127-133
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• 2017

In this study, to develop the adhesives with improved waterproof properties for display application, UV-curable urethane acrylate oligomers containing a fluorine moiety were synthesized from hydrogenated polybutadiene diol (HLBH-P2000), perfluoropolyether diol (HTPFPE), isophorone diisocyanate (IPDI) and 2-hydroxyethyl acrylate (HEA). The properties which were related waterproof performance such as contact angle, surface energy and water vapor transmission ratio (WVTR) of synthesized fluorine induced urethane acrylate oligomers were measured. As the HTPFPE content increased, the contact angle also increased from 97.9 degree to 104.4 degree. The surface energy and WVTR decreased as the HTPFPE content increased. The adhesion strength also decreased according to the HTPFPE content increased, and the adhesion strength reduction had greatly increased as the HTPFPE content increased. The findings of this study will be beneficial for developing waterproofing adhesives in the field of display which has recently been a waterproof issue.

• Horticultural Science & Technology
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• v.30 no.2
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• pp.224-228
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• 2012

A new $Saxifraga$ $fortunei$ cultivar 'Crystal' was bred by crossing 'Dotaerang' with light pink and light Green (RP 58D) colored petals and 'Rocksun' with light pink and light green white (RP62A + GW157A) colored petals. The selection and flower characterization were conducted from 2004 to 2007 in a greenhouse of the National Institute of Highland Agriculture. The flowering of 'Crystal' occurred from September 21 for 31 days. The number of flowers per plant was 279.0 with many flowering habit. The flower coloration was mixed light pink and green white (RP62D + GW157B) at the opening. The number of petals were 7.0, with a width of 2.1 cm and length of 1.8 cm. The plant height was 15.3 cm and number of leaves was 133.7. 'Crystal' cultivar can be used for pot flower. Fifty percent shading is a good cultural practice against direct sunlight and soft rot disease would occurs usually by over-irrigation. 'Crystal' cultivar was registered as a new variety with No. 3466 at Korea Seed and Variety Service in April of 2011 year.

• Horticultural Science & Technology
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• v.30 no.3
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• pp.329-333
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• 2012

A new Asiatic hybrid lily 'Redsky' cultivar was selected from a cross between 'Vivaldi' cultivar with pale pink color flower as mother plant and 'Prato' with orange colored flower in 2000 year. The selected bulb line was propagated and cultivated for line nomenclature as 'Gonong-F-na-10'. The survey of the growth and flower characteristics of 'Gonong-F-na-10' were conducted from 2005 to 2007 on summer cropping system under structure in Highland Agriculture Research Center, NICS, RDA. After all 'Gonong-F-na-10' was named as 'Redsky'. The flowers of the 'Redsky' cultivar had not soft hair and fragrance, and had orange red (O-R N30D) colored petals with dark brown spots in center of flower. The stem color was dark pink and brown caused by anthocyanin pigment, the stigma and pollen colors were brown. Flowering direction was upward. The flowering date was July 7 so early flowering. The plant height was 97.2 cm so long, number of flowers was 4.9 ea, and length of flower bud was 9.3 cm. The bulb weight was about 34.8 g and its bulb size was about 14.8 cm in circumference. 'Redsky' cultivar was registered as a new variety with No. 3119 in Korea Seed and Variety Service on June of 2010.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.35 no.4
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• pp.353-358
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• 2011

In this paper, we describe a process for optimally designing a ring-type permanent magnet thrust bearing. The bearing consists of two sets of permanent magnet rings. One set is located inside the other set. An axial offset between the two sets creates axial force, which results in a thrust bearing function. In order to realize an optimal design of the bearing where the required load capacity of the bearing is achieved with the least magnet volume, we derived analytical design equations by adopting the equivalent current sheet (ECS) method. We considered the following two types of magnet arrays: axial arrays and Halbach arrays. These two types of arrays are optimized using the analytical design equations. The results of the optimization are verified using three dimensional (3D) finite element analyses (FEA). The results show that the Halbach array can achieve the required load capacity with less amount of permanent magnet than the axial array does. The efficacy of the ECS method is also verified by using 3D FEA. It is found that the accuracy of ECS method is more sensitive to the underlying assumptions for the Halbach array than for the axial array.

• Journal of Korean Society for Geospatial Information Science
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• v.19 no.3
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• pp.3-11
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• 2011

The objective of this study was to select appropriate sites for reforestation CDM using satellite image and spatial data in North Korea. A suitable site for reforestation CDM can be defined as non-forest area since 1990. To detect land cover change between two different years and to delineate potential reforestation CDM site(unstocked forest, converted crop land, denuded bare land), Landsat TM satellite image which was scanned in 1988 and SPOT Pan-sharpened image which was scanned in 2007 were used. As a result of classification, 1,214 ha of forest area in 1989 had been converted to other land cover types in 2007. and 2,245 ha of the total study area was detected to be suitable for reforestation CDM. 79.2% of total potential CDM sites was converted crop land. Through topography and accessibility analysis for potential reforestation CDM sites detected by satellite image, suitability index was calculated and the potential reforestation area was reclassified into suitability grades.

• Tuberculosis and Respiratory Diseases
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• v.62 no.1
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• pp.43-50
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• 2007

Background: Mutated or deregulated expression of C-erbB-2 causes this gene to function as a potent oncogene. Vascular endothelial growth factor (VEGF) is a crucial angiogenic molecule in lung cancer. Both C-erbB-2 and VEGF can promote growth, proliferation and metastasis in non-small cell lung cancer (NSCLC). The purpose of this study was to investigate evaluate the relationship between the expressions of the C-erbB-2 and VEGF genes using immunohistochemistry. Materials and Methods: Ninety-five patients with NSCLC were involved (60 squamous cell carcinoma and 35 adenocarcinoma). The formalin-fixed paraffin embedded specimens were immunohistochemically stained for C-erbB-2 and VEGF using the avidin-biotin complex method. Results: Positive C-erbB-2 expression was observed more often in adenocarcinomas than squamous cell carcinomas (p<0.05). Although the immunohistochemical expressions of C-erbB-2 and VEGF in non-small-cell lung cancer showed increased tendencies at an advanced stage, the correlation between early and advanced cancers was insignificant. In adenocarcinomas, the expressions of VEGF and C-erbB-2 were significantly (p<0.05). Conclusion: The overexpression fo C-erbB-2 was significantly higher in adenocarcinomas than squamous cell carcinomas, and correlated with the expression of VEGF in adenocarcinomas of the lungs.

• Korean Journal of Plant Resources
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• v.23 no.4
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• pp.379-385
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• 2010

This study was carried out to develop cultivation techniques of Climacium japonicum in transparent pot. We analyzed effect of different soil moisture content, air exposure degree, bed soil and ground cover moss on the growth of Climacium japonicum. The best humidity condition in transparent pot culture was 60~80% and a mixture of bed soil with peatmoss : perlite as 7:3 ratio was effective to growth. Two holes(each diameter is 4 mm) in the cover were also effective to the growth and control moisture in the transparent pot. The best mixture of bed soil was moss with peatmoss : pelite(7:3) in the pot culture but price of moss is expensive, the moss treatment was not practical. Total of 10 moss species were selected as ground cover plant; Hypnum erectiusculum Sull. et Lesq., Thuidium kanedae Sak., Hypnum plumaeforme Wils., Trachycystis microphylla Lindb., Bryum argenteum Hedw., Hypnum oldhamii Jaeg., Funaria hygrometrica Hedw., Leucobryum glaucum Aongstr., Polytrichum commune Hedw. and Weissia controversa Hedw. The plant height of selected moss was smaller than that of Climacium japonicum so these moss were very effective to cover the ground.