• Title/Summary/Keyword: Yield line method

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Application of Oxygen Uptake Rate Measured by a Dynamic Method for Analysis of Related Fermentation Parameters in Cyclosporin A Fermentation:Suspended and Immobilized Cell Cultures

  • Chun, Gie-Taek;Agathos, S.N.
    • Journal of Microbiology and Biotechnology
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    • v.11 no.6
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    • pp.1055-1060
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    • 2001
  • Experimental data for the on-line estimation of cell concentration and growth rate are presented. For this purpose, we utilized the on-line calculation of the oxygen uptake rate (OUR), which was derived from a liquid phase dynamic mass balance for the oxygen during the active growth phase in cyclosporin A (CyA) fermentation. The cell yield coefficient, based on the oxygen $(Y_{x/o})$for both suspended and immobilized cells of Tolypocladium inflatum, was estimated as $1.9 gDCW/gO_2$ from a very good linear correlation between the cell mass produced and the total oxygen consumed. The calculated yield showed a good agreement with the value of $(Y_{x/o})$ generated from the correlation between the cell growth rate and the oxygen uptake rate. In addition, further experimental data are given, which were also applied to determine the specific oxygen uptake rate of T. inflatum cells during the exponential phase of CyA fermentation. A theoretical basis for the analysis of these fermentation parameters is also provided.

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FEA based optimization of semi-submersible floater considering buckling and yield strength

  • Jang, Beom-Seon;Kim, Jae Dong;Park, Tae-Yoon;Jeon, Sang Bae
    • International Journal of Naval Architecture and Ocean Engineering
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    • v.11 no.1
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    • pp.82-96
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    • 2019
  • A semi-submersible structure has been widely used for offshore drilling and production of oil and gas. The small water plane area makes the structure very sensitive to weight increase in terms of payload and stability. Therefore, it is necessary to lighten the substructure from the early design stage. This study aims at an optimization of hull structure based on a sophisticated yield and buckling strength in accordance with classification rules. An in-house strength assessment system is developed to automate the procedure such as a generation of buckling panels, a collection of required panel information, automatic buckling and yield check and so on. The developed system enables an automatic yield and buckling strength check of all panels composing the hull structure at each iteration of the optimization. Design variables are plate thickness and stiffener section profiles. In order to overcome the difficulty of large number of design variables and the computational burden of FE analysis, various methods are proposed. The steepest descent method is selected as the optimization algorithm for an efficient search. For a reduction of the number of design variables and a direct application to practical design, the stiffener section variable is determined by selecting one from a pre-defined standard library. Plate thickness is also discretized at 0.5t interval. The number of FE analysis is reduced by using equations to analytically estimating the stress changes in gradient calculation and line search steps. As an endeavor to robust optimization, the number of design variables to be simultaneously optimized is divided by grouping the scantling variables by the plane. A sequential optimization is performed group by group. As a verification example, a central column of a semi-submersible structure is optimized and compared with a conventional optimization of all design variables at once.

Study on the Compositions of Photosensitive Ag Paste for Patterning Embedded Fine-Line Inductor in LTCC (LTCC 내장형 미세 라인 인덕터 구현을 위한 감광성 Ag Paste 조성에 관한 연구)

  • Lee, Sang-Myoung;Park, Seong-Dae;Yoo, Myong-Jae;Lee, Woo-Sung;Kang, Nam-Kee;Nahm, Sahn
    • Journal of the Korean Ceramic Society
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    • v.44 no.3 s.298
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    • pp.157-161
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    • 2007
  • Line width under $100\;{\mu}m$ with good resolution is difficult to achieve using conventional thick-film process utilizing screen printing method. However combined with lithography technology finer line and space for miniaturization and highly integrated package is achievable. In this study, photosensitive Ag paste of optimum formulation used for thick film lithography technology was fabricated by various Ag powder, glass powder and additives. As the result, line width of $30\;{\mu}m$ with good definition and reduced mismatch during co-firing with LTCC substrate was acquired. Formulated Ag paste was used to pattern embedded fine line inductor with over 90% yield.

Study on Plastic Deformation of Interior Support at the Continuous I-Beam Bridge (I-Beam연속교 내측지점의 소성변형에 관한 연구)

  • Chung, Kyung-Hee;Kim, Jin-Sung;Yang, Seung-Ie
    • Journal of the Korean Society of Safety
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    • v.17 no.4
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    • pp.146-152
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    • 2002
  • The steel shows plastic deformation after the yield point exceeds. Because of overloads, the plastic deformation occurs at the interior support of a continuous bridge. The plastic deformation is concentrated at the interior support, and the permanence deformation at the interior support remains after loads pass. Because local yielding causes the positive moment at the interior support, it is called "auto moment". Auto moment redistributes the elastic moment. Because of redistribution, auto moment decreases the negative moment at the interior support of a continuous bridge. In this paper, the moment-rotation curve from Schalling is used. The Plastic rotation is computed by using Beam-line method, and auto moment is calculated based on the experiment curve. The design example is presented using limit state criterion.

Enhanced extraction and Antioxidant activity analysis of Flavone C-glycosides from Black bamboo using Ultrasonic wave (초음파를 이용한 오죽으로부터 Flavone C-glycosioes의 추출 및 항산화활성분석 향상)

  • Choi, Sun-Do;Lee, Kwang-Jin
    • KSBB Journal
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    • v.23 no.4
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    • pp.297-302
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    • 2008
  • In this work, the amounts of Flavone C-glycosides homoorientin, orientin extracted from Black bamboo by various ultrasonic waves frequency (35, 72, 170 KHz, 300 Watt $\pm$1) time (15, 30, 60 min) and temperature ($25^{\circ}C$) were compared using 50% aqueous ethanol solution. And describesanalysis of the antioxidant potential of Black bamboo using an high-performance liquid chromatography (HPLC) on-line $ABTS^+$antioxidant screening method. In conjunction with the analysis of their 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid ($ABTS^+$) radical scavenging ability. The optimum operating conditions were experimentally determined to analyze the Flavone C-glycosides homoorientin, orientin in the pretreatment ultrasonic extracts. From the results, the effect on extraction yield of variations in frequency, solvent composition and extraction time was investigated. The highest yield of Black bamboo was obtained by ultrasonic waves with a frequency of 72 KHz and an extraction time of 60 min, The extraction frequency 35 KHz ($25^{\circ}C$), time 30 min was selected as an optimal antioxidant activity condition.

Stereo Matching Algorithm Based on Line Constraint and Reliability Space (신뢰도 공간과 선형 제어를 통한 스테레오 정합 기법)

  • An, Xiao-Wei;Han, Young-Joon;Hahn, Hern-Soo
    • Proceedings of the Korean Society of Computer Information Conference
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    • 2011.01a
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    • pp.59-62
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    • 2011
  • A new method is proposed for stereo vision where solution to disparity map is presented in terms of Line constraint and Reliability space -- the first constraint proposes a progressive framework for stereo matching which applies local area pixel-values from corresponding lines in the left and right image pairs. The second states that reliability space based on corresponding points records the disparity and then we are able to apply the median filter in order to reduce the noises which occur in the process. A coarse to fine result is presented after the median filtering, which improves the final result qualitatively. Experiment is evaluated by rectified stereo matching images pairs from Middlebury datasets and has proved that those two adopted strategies yield good matching quantitative results in terms of fast running speed.

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Evaluation of Rotation Capacity of Steel Moment Connections ConsideringInelastic Local Buckling - Model Development (비탄성 국부좌굴을 고려한 철골 모멘트 접합부 회전능력 평가를 위한 모델 개발)

  • Lee, Kyung Koo
    • Journal of Korean Society of Steel Construction
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    • v.20 no.5
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    • pp.617-624
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    • 2008
  • Well-designed steel moment connections will undergo local buckling before they exhaust their available rotation capacity, and inelastic post-buckling deformation plays a major role in defining the connection rotation capacity. An approximate analytical method to model strength degradation and failure of beam plastic hinges due to local buckling and estimation of the seismic rotation capacity of fully restrained beam-column connections in special steel moment-resisting frames under both monotonic and cyclic loading conditions is proposed in this study. This method is based on the plastic mechanism and a yield line plastic hinge (YLPH) model whose geometry is determined using the shapes of the buckled plastic hinges observed in experiments. The proposed YLPH model was developed for the improved WUF-W and RBS connections and validated in comparison with experimental data. The effects of the beam section geometric parameters on the rotation capacity were discussed in the companion paper (parametric studies).

Increase of Cell Concentration by the Automatic Addition of Glucose and Ammonium to an Alcohol Distillery Wastewater Reutilized for Cultivating a Baker's Yeast : Automatic Analysis and Control of Ammonium Concentration with an On-line Flow Injection Analysis System (알콜증류폐액을 이용한 빵효모배양에서 Glucose와 Ammonium의 자동첨가에 의한 증균 : 온라인 FIA 시스템에 의한 Ammonium의 자동분석 및 제어)

  • 이형춘
    • KSBB Journal
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    • v.15 no.2
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    • pp.139-144
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    • 2000
  • Automatic addition of glucose and ammonium to an alcohol distille깨 wastewater and the control of them at low $\infty$ncentration were tried to efficiently increase the cell concentration of a baker's yeast c비tivated in that wastewater. Added g glucose was indirectly controlled to less than 116 mg/L by a method which used DO as control parameter. Ammonium was a automatically measured and controlled within the range of 7.0~27.7 mM by a homemade on-line system which adopted FIA a as measurement method. Maximum specific growth rate and biomass yield to glucose were $0.21 hr^{-1}$ and about 0.78 g/g, w which were significantly increased values in contrast to those of an experiment without ammonium control. Biomass yield to a ammonium was 11.3 gIg. Cell cone엉ntration could be increased from 2.6 g/L to 18.5 g/L by the add ion of glucose and a ammonium.

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Calculation of Failure Load of V-shaped Rock Notch Using Slip-line Method (Slip-line법을 이용한 V형 암석 노치의 파괴하중 계산)

  • Lee, Youn-Kyou
    • Tunnel and Underground Space
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    • v.30 no.4
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    • pp.404-416
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    • 2020
  • An analytical procedure for calculating the failure load of a V-shaped rock notch under two-dimensional stress conditions was developed based on the slip-line plastic analysis method. The key idea utilized in the development is the fact that the α-line, one of the slip-lines, extends from the rock notch surface to the horizontal surface outside the notch when the rock around the notch is in the plastic state, and that there exists an invariant which is constant along the α-line. Since the stress boundary condition of the horizontal surface outside the rock notch is known, it is possible to calculate the normal and shear stresses acting on the rock notch surface by solving the invariant equation. The notch failure load exerted by the wedge was calculated using the calculated stress components for the notch surface. Rock notch failure analysis was performed by applying the developed analytical procedure. The analysis results show that the failure load of the rock notch increases with exponential nonlinearity as the angle of the notch and the friction of the notch surface increase. The analytical procedure developed in this study is expected to have applications to the study of fracture initiation in rocks through wedge-shaped notch formation, calculation of bearing capacity of the rock foundation, and stability analysis of rock slopes and circular tunnels.

Studies on the Manifestation of Hybrid vigour and Combining Ability in Polyvoltine X Bivoltine (cross breed) Hybrids of Mulberry Silkworm Bombyx mori L.

  • Seshagiri, S.V.;Naik, S. Sankar;Seetharamulu, J.;Goel, A.K.;Raju, P.J.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.24 no.1
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    • pp.7-16
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    • 2012
  • To meet the local requirement i.e. the tropical stress of Andhra Pradesh and marginal farmers, twenty four new hybrid combinations were tested in Line x Tester method involving eight newly developed polyvoltine oval lines viz., MSO1, MSO2, MSO3, MSO4, MSO5, MSO6, MSO7 and MSO8 and three bivoltine silkworm breeds viz., APS12, APS45 and APS8 as testers. These hybrids were reared to evaluate their performance. Data for eight economically important metric traits viz., fecundity, cocoon yield per 10,000 larvae by number, cocoon yield per 10,000 larvae by weight, survival rate, cocoon weight, cocoon shell weight, cocoon shell ratio and filament length was recorded. General combining ability (gca), specific combining ability (sca) and heterosis were analyzed. Further, the relative merit of the hybrids for all the traits under study was also assessed adopting Evaluation Index method. Based on combining ability effects, heterosis and evaluation index, five hybrid combinations viz., MSO3 $\times$ APS45, MSO1 $\times$ APS12, MSO7 $\times$ APS8, MSO2 $\times$ APS45 and MSO1 $\times$ APS45 were selected for further evaluation in different seasons.