• Journal of the Korean Society of Food Science and Nutrition
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• v.32 no.1
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• pp.35-41
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• 2003

The study was conducted to develope a rapid method for the detection of Yersinia enterocolitica in spring water and vegetables via multiplex polymerase chain reaction (PCR) technique using ail, yst, uirF and subgenus-specific Y16S primers. Specificity and sensitivity of multiplex PCR and application of best primers for the detection of Y. enterocolitica from spring water and vegetables were investigeted. Y. enterocolitica ATCC 27729 strains gave 356 bP and 200 bp (Y16S) and 134 bp (yst) bands. but Y. enterocolitica ATCC 9610 and ATCC 23715 strains gave 200 bp and 134 bp bands.In the meanwhile, non-pathogenic Yersinia species, such as Y. frederikseni, Y. inter-media, Y. kristenseni and Y. pseudotuberculosis gave only single 200 bp band, and other bacteria including Escherichia coli O157:H7 ATCC 25392, Shigella dysenteri. Staphylococcu aureus ATCC 25923 and Listeria mo-nocytogenes ATCC 19111 did not show any bands. Among primers, yst and Y16S primer showed the best sensitivity. Seven CFU/mL Y. enterocolitica cells could be detected with yst and Y16S primers and the sensitivity was significantly improved by the further 2nd PCR after 38 cycles of first PCR amplication. Spring water, cabbage and mushroom were inoculated with Y. enterocolitica to determine the sensitivity of multiplex-PCR for the rapid detection of Y. enterocolitica. Multiplex-PCR assay could detect 7 or 70 cells in spring water and vegetables using whole cell lysate with repeating PCR amplication.

• The Journal of the Korean Society for Microbiology
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• v.15 no.1
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• pp.3-8
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• 1980

Yersinia enterocolitica has been known to be an important enteric pathogen especially in Scandinavian countries and Canada. In Korea, the authors reported the first case of Y. pseudotuberculosis septicemia in 1979. In 1980, three isolates of Y enterocolitica were obtained from 3 adult patients with enteritis, besides the already reported one in a 5-month-old child, during March to June 1980. Difficulty in the isolation was experienced; ie., the organism was isolated only from the SS primary isolation plate in one case and in the other two cases only from the SS plates inoculated with overnight culture of selenite broth. The isolates showed typical cultural and biochemical characteristics except for the nonmotility even at room temperature. Two isolates were indole negative possibly belonging to Wauter's biotype 3 and the other one was indole positive belonging to biotype 2. One patient was tested for the serum agglutinin titer on the 8th hospital day and it was found to be 1:128. All of the isolates were susceptible to chloramphenicol, colistin, gentamicin, kanamycin, tetracyclne, and tobramycin by the Kirby-Bauer disc diffusion method. All of the infections were controled by ampicillin, amoxicillin, amikacin, or gentamicin treatment. It is considered urgent to broaden our knowledge on yersiniosis in Korea not only by isolating, serotyping and biotyping of the organism, but also by surveying serum agglutinin titer of enteritis patients and normal individuals.

• Journal of the korean veterinary medical association
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• v.38 no.9
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• pp.781-800
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• 2002

Recently, biological weapons (BWs) prepared with pathogenic microorganisms, toxins and biological vectors have been used maliciously for biological warfare, bioterrorism and/or agroterrorism by hostile countries and terrorists. In this review, historical background of disease and malicious use of BWs pathogenicity of microorganisms, advanced methodology involved in laboratory diagnosis, and prevention and control of anthrax (Bacillus anthracis), plague (Yersinia pseudotuberculosis subs. pestis), glanders (Burkholderia mallei), and smallpox (Variola virus) which have been abused for biological warfare or bioterrorism were discussed. In addition, the pathogenicity of microorganisms and the methodology needed to diagnose and control 6 diseases identified by WHO/CDC, ie., smallpox, inhalation anthrax, pneumonic plague, botulism, tularemia, and hemorrhagic fevers that would wreak havoc if terrorists successfully disseminated the germs by air were described.

• Journal of Microbiology and Biotechnology
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• v.26 no.6
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• pp.1115-1123
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• 2016

_L-Asparaginase (E.C. 3.5.1.1) is an enzyme involved in asparagine hydrolysis and has the potential to effect leukemic cells and various other cancer cells. We identified the _L-asparaginase gene (_L-ASPG86) in the genus Mesoflavibacter, which consists of a 1,035 bp open reading frame encoding 344 amino acids. Following phylogenetic analysis, the deduced amino acid sequence of _L-ASPG86 (_L-ASPG86) was grouped as a type I asparaginase with respective homologs in Escherichia coli and Yersinia pseudotuberculosis. The _L-ASPG86 gene was cloned into the pET-16b vector to express the respective protein in E. coli BL21 (DE3) cells. Recombinant _L-asparaginase (r-_L-ASPG86) showed optimum conditions at 37-40℃, pH 9. Moreover, r-_L-ASPG86 did not exhibit glutaminase activity. In the metal ions test, its enzymatic activity was highly improved upon addition of 5 mM manganese (3.97-fold) and magnesium (3.35-fold) compared with the untreated control. The specific activity of r-_L-ASPG86 was 687.1 units/mg under optimum conditions (37℃, pH 9, and 5 mM MnSO₄).