• 한국응용과학기술학회지
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• 제36권4호
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• pp.1338-1348
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• 2019

The Geomunoreum Lava Tube System, declared as a UNESCO World Heritage Site, has a unique natural ecosystem. The information available about this ecosystem, which contains lava caves with secondary carbonate speleothems, is sparse. Hence, extensive research is warranted for establishing a conservation standard. We commenced microbial research on the system and have been studying the microorganisms coating the lava tube wall to acquire fundamental information for understanding the lava cave ecology of Jeju Island. Samples were collected from yellow-colored walls in six caves that are part of the system-the Bengdwi, Utsanjeon, Bukoreum, Manjang, Gimnyeong, and Yongcheon caves. This study focused on yellow walls as it is the most easily distinguished color. According to previous studies, the color of cave walls is attributed to microorganisms or their components. To determine whether the yellow mats from the Jeju lava tube walls are caused by microorganisms, we examined samples at the microscopic scale, by staining mats and analyzing bacterial isolates from glitter particles. As a result, we found that the yellow walls of lava tubes are comprised of microbial mats.

• 동아시아식생활학회지
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• 제20권2호
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• pp.272-278
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• 2010

Various quality attributes were determined in muffins prepared using purple-colored sweet potato flour. Height of control muffins was 5.8 cm lease clarify. Height decreased with increasing quantity of sweet potato powder. Muffin volume was not appreciably affected. Moisture of control muffins was 21.84. The moisture value decreased with increasing quantity of sweet potato powder. Scanning electron microscopy examination revealed thick cell wall and rough stoma in muffins prepared with purple-colored sweet potato powder. The amount of gluten decreased as the quantity of purple-colored sweet potato powder increased. Brightness L values significantly changed as the amount of powder increased, indicative of a progressively darker product. Red index a values decreased as the quantity of purple-colored sweet potato powder increased. Yellow index b values decreased significantly in the control group as powder quantity increased. Hardness increased with increasing addition of powder, but no significant difference was evident between 6% and 9% amended samples. There were also no significant differences in springing in samples containing 3% and 6% powder but 9% powder produced a significant difference. Cohesiveness did not differ in unamended samples and samples amended with 3% and 9% powder, however a significant difference was evident in samples prepared with 6% powder. Gumminess and chewiness increased as the quantity of purple-colored sweet potato powder increased. Color did not differ appreciably in sample prepared with 3% and 9% Sample prepared with 3% additives showed the highest What? Color score? Something else? Please clarify with 6.4. There was no significant difference of the flavor in samples prepared with 3% and 6% powder, or in taste among samples prepared 3%, 6%, and 9% powder. No significant differences in texture were evident in any sample Overall acceptability was highest (7.2) in samples prepared with 3% of purple-colored sweet potato.

• 한국수산과학회지
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• 제31권4호
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• pp.516-522
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• 1998

검정해면 (Halichondlia okadai)에 공생하는 것으로 추정되는 항균성을 가진 방선균주를 분리하여 SEM, 형태학적, 화학 분류학적 방법에 의하여 균주를 동정하였으며, 이 균주를 marin broth 2216 배지에서 대량 b배양하여 균사체로부터 항균물질을 각종 HPLC에 의하여 분리하고 X선 결정 구조 해석. MS, NMR spectrum에 의하여 구조를 확인하였다. ISP 2 배지에서 $30^{\circ}C$, 7일간 배양 결과, 기질균사는 갈색을 띄었고 그 위로 ivory색의 기중균사를 형성하였다. spore chain은 flexibilis로서 10개 이상의 oval 형의 포자가 사슬을 형성하고 있었으며 표면은 smooth하였다. 한편. 화학적 특성으로는 L-form의 DAP를 가지며 특징적인 당이 없는 cell wall type I 이었고. 인지질은 PII형 이었으며, 주요 지방산은 iso-14 : 0, anteiso-15 : 0, iso-16 : 0, 16 : 0 및 iso-17:0 이었다. 이들 결과와 Bergey's Manual에 의해 이 균주는 Strptomyces sp.로 동정하였다. 대량 배양한 동결 건조 균체 70g으로 부터 10mg의 연한 노란색의 항균 물질을 분리 정제하였다. 질량 분석에서 이 물질의 분자량은 466으로 확인되었고, 이를 benzene-acetone 용매중에서 결정화하여 X선 결정 구조 해석을 행한 결과 indolo[2,3-a]carbazole alkaloid의 staurosporine 으로 판명되었으며 proton 및 carbon NMR data도 이와 잘 일치하였다.