• 한방안이비인후피부과학회지
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• 제20권2호통권33호
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• pp.102-107
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• 2007

Objectives : This experiment study was performed to investigate the antioxidant effects of Yeouigeumhwang-san(YUGHS). Methods : Antioxidant effects of YUGHS on HaCaT cell, that were measured by the scavenging for DPPH radical, the formation of intracellular GSH and the inhibition for ROS. Results : 1. YUGHS showed an oxidation inhibitory effect by decreasing the DPPH radicals. 2. Concentrations of 20 ${\mu}g/ml$, 50 ${\mu}g/ml$ YUGHS slightly increased the formation of intracellular GSH in HaCaT cell. 3. Concentration of 50 ${\mu}g/ml$ YUGHS slightly inhibited the production of ROS in the HaCaT cell stimulated with $H_2O_2$, but it is not significant. Conclusions : These results indicate that YUGHS has antioxidant effects. If further study is performed, the use of YUGHS will be valuable and benificial in the therapy of inflammation.

• 한방안이비인후피부과학회지
• /
• 제20권2호통권33호
• /
• pp.77-88
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• 2007

Objectives : This experimental study was performed to investigate the effects of Yeouigeumhwang-san(YUGHS) on anti-inflammation and anti-Propionibacterium acnes. Methods : The cytotoxicity of YUGHS about viability of Raw 264.7 cell was tested by using a colorimetric tetrazolium assay(MTT assay). To investigate the anti-inflammatory effets of YUGHS on LPS-induced macrophage Raw 264.7 cell, we used ELISA kit and Western blots. Inhibitory effects of YUGHS on Propionibactrium acnes were investigated by using paper disk diffusion method. Results : 1. YUGHS has no cytotoxicity under 50 ${\mu}g/ml$ concentration but over 50 ${\mu}g/ml$ has a little cytotoxicity in Raw 264.7 cell. 2. Concentration of 100 ${\mu}g/ml$ YUGHS inhibited the production of NO in the Raw 264.7 cell stimulated with LPS. 3. All concentrations of YUGHS did not inhibit the production of $TNF-{\alpha}$ in the Raw 264.7 cell stimulated with LPS. 4. All concentrations of YUGHS significantly inhibited the production of $PGE_2$ in the Raw 264.7 cell stimulated with LPS. 5. YUGHS did not inhibit the expression of COX-2 but concentration of 50 ${\mu}g/ml$ YUGHS inhibited iNOS expression in the Raw 264.7 cell stimulated with LPS. 6. YUGHS has the effect of blocking $NF-{\kappa}B$ into nucleus in LPS-induced macrophage Raw 264.7 cell 7. YUGHS did not have the inhibitory effect of Propionibactrium acnes. Conclusions : These results indicate that Yeouigeumhwang-san has anti-inflammatory effets. If further study is performed, the use of Yeouigeumhwang-san will be valuable and benificial in the therapy of acnes.