• Korean Journal of Soil Science and Fertilizer
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• v.32 no.1
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• pp.76-83
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• 1999

Studies were conducted during 2 months from May of 1997 to evaluate the effects of pig manure compost(PMC) on soil microbial flora. To do so, a field experiment of Chinese cabbage(Brassica campestris L.) was conducted in a randomized block design on a sandy loam soil and microbial floral characteristics in soils were analyzed. Treatments to control included the application of PMC at (A) $8Mg\;ha^{-1}$CM-8), (B) $29Mg\;ha^{-1}$(CM-2,9), and (C) $57Mg\;ha^{-1}$(CM-57), and of chemical fertilizer(D) at $320N-80P_2O_5-200K_2O\;kg\;ha^{-1}$(NPK). In each treatment, the rhizosphere and non-rhizosphere soils were tested for the analysis of microbial populations. The populations of bacteria, actinomycetes, and fungi increased in soils with the applications of PMC and chemical fertilizer, but that of Bacillus sp. decreased. However, the population of fluorescent Pseudomonas sp. was reduced in NPK plots only. With increasing application rates of PMC, the number of colony forming units(cfu) of bacteria (Pseudomonas sp. and actinomycetes) and fungi increased. in all PMC-treated plots, the population density peaked at early growth stage for bacteria(including Bacillus sp.), at late growth for fluorscent Pseudomonas sp., and at harvest for fungi and actinomycetes. The rhizosphere effect was greatest for fluorscent Pseudomonas sp. As the application rates of PMC increased, Total N, organic matter, available phosphate, and exchangeable -K, -Ca, and -Mg increased compared to control, but soil pH was lowered. In NPK plots, EC was 3.4-fold and exchangeable K was 5-fold higher than control.

• Korean Journal of Soil Science and Fertilizer
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• v.32 no.1
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• pp.22-30
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• 1999

This experiment was carried out to determine the forage yield and mineral contents of silage corn(Zea mays L.), and N balance, soil chemical characteristics and nitrate-N level in infiltration water by different types and N rates of liquid manure applied. Seven treatments consisting of chemical fertilizer $200kg\;N;ha^{-1}$, liquid cattle manure 200, 320 and $440kg\;N;ha^{-1}$, liquid swine manure 200, 320 and $440kg\;N;ha^{-1}$ were arranged in a randomized complete block design with three replications. The results obtained at National Livestock Research Institute, RDA, in Suweon from May 1997 to Aug. 1997 are summarized as follows : There were no significant differences in percentage of dry matter among the treatments, but significant dry matter yield differences were found (P<0.05), and also the mean dry matter yield of liquid swine manure plot was higher than that of liquid cattle manure plot. Potassium, calcium and magnesium contents of silage corn increased with increasing liquid manure application rates (P<0.05). Nitrate-N content of silage corn in the liquid cattle manure treatments was not influenced by liquid manure application rates, but that in the liquid swine manure treatments increased with increasing the N rates of liquid manure application. pH and contents of exchangeable canons of the soil after experiment were raised by increasing the amount of liquid manure application. Available $P_2O_5$ content in topsoil(0-10cm) was highest as $340mg\;kg^{-1}$ at the plot of liquid swine manure $440kg\;N;ha^{-1}$. Concentration of nitrate-N in infiltration water increased at the plot of liquid swine manure $440kg\;N;ha^{-1}$. Amount of nitrogen balance increased with increasing liquid manure application rates. Based on the results of this experiment, it is suggested that the mean dry matter yield of liquid swine manure plot was higher than that of liquid cattle manure plot, and the amount of nitrogen balance increased with increasing liquid manure application.

• Tuberculosis and Respiratory Diseases
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• v.58 no.2
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• pp.120-128
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• 2005

Background : Insulin-like growth factor binding protein (IGFBP)-3 regulates non-small cell lung cancer(NSCLC) cell proliferation in vitro and in vivo by inhibiting IGF-mediated signaling pathways. To have better strategies for the treatment of lung cancer, we analyzed the combining effects of adenovirus expressing IGFBP-3 (Ad5CMV-BP3) and SCH66336, a farnesyl transferase inhibitor (FTI) designed to block Ras-mediated proliferative signaling pathways. Methods : To measure the combining effects of Ad5CMV-BP3 and SCH66336 on the proliferation of NSCLC cells, human NSCLC cell lines (H1299, H596, A549, H460, and H358), SCH66336, recombinant adenovirus expressing IGFBP-3 (Ad5CMV-BP3) and athymic nude mice were used in these experiments. Results : The combination of Ad5CMV-BP3 and SCH66336 produced a synergistic enhancement in antiproliferative effects over a range of clinically achievable concentrations in a variety of NSCLC cell lines. Furthermore, we observed a significant reduction in growth of NSCLC xenograft induced in athymic nude mice. Conclusion : In conclusion, this study demonstrated for the first time that the FTI SCH66336 synergizes with IGFBP-3 and enhances its apoptotic activity in NSCLC cells in vitro and in vivo. The combined treatment of Ad5CMV-BP3 and SCH66336 raises the possibility of using this regimen in clinic for the treatment of NSCLC.

• Journal of The Korean Society of Grassland and Forage Science
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• v.19 no.1
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• pp.17-22
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• 1999

Experiment was carried out to determine the agronomic characteristics, forage yield of silage corn(Zea mays L.) soil chemical characterictics and $NO_3-N$ level in infiltration water by different application rates of daily liquid manures. Four treatment consisting of chemical fetilizer, $200kg\;N\;ha^{-1}$, daily liquid manure $200kg\;N\;ha^{-1}$, $300kg\;N\;ha^{-1}$ and $400kg\;N\;ha^{-1}$ were arranged in a randonmized complete block design with three replicates. The results obtained at National Livestock Research Institute, RDA., in Suwon from 1996 to 1997 are summarized as follows; In plant height and crude protein content, it were appeared to highest by 264cm, 6.8% at the cattle slurry 150% plot(T3), respectively. but Dry matter yield(14.5MT/ha) and TDN(9.5MT/ha) production of chemical fertilizer plot(T1) were highest. Among daily liquid manure amount, dry matter yield(14.0MT/ha) and TDN yield(9.1MT/ha) of daily liquid manure 100% plot(T2) were resulted to 96% and 97% of chemical fertilizer plot(T1). End year's soil organic matter and phosphate content were appeared to high than those of beginning year in daily liquid manure plots, but it was not in proportioned to increase according to slurry amount. $NO_3-N$ level in infiltration water lower than 60cm of all plots were lowed than a permitted limit of cattle drinkable. As a result, it was appeared that the optimum application amount of dairy liquid manure was 200kg/ha by manure-N.

• Journal of The Korean Society of Grassland and Forage Science
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• v.19 no.1
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• pp.41-48
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• 1999

This experiment was carried out to determine the effect of the silage additives on improvement of quality of fresh rice straw silage using round bale at the forage experimental field, grassland and forage crops division, National Livestock Research Institute, RDA, Suwon from 1997 to 1998. The experiment was arranged in a randomized block design with three replication. The treatments used in this study were consisted of different additives(control, formic acid, molasses, molasses+urea and inoculant). The rice straw silage with molasses+urea treatment resulted in high crude protein content and in vitro dry matter digestibility were increased with molasses of inoculant treatments compare with the control. The mean dry matter of formic acid treatment material was higher than with control but there was no significant difference in dry matter content among the additives treatments. The pH of molasses treatments significantly increased the proportion of lactic acid(P<0.05) and decreased the proportion of butyric acid. The total organic acid content of all treatments had low around 2%. Ammonia-N of molasses+urea treatment was significantly(P<0.05) higher than that of others, but formic acid or inoculant treatments was lower below 10% per total nitrogen. Over a 7d feeding period, the dry matter intake per cattle on the inoculant treatment was higher that on both the untreated round bale silage of fresh rice straw and rice straw hay. Producing cost per kilogram of round bale silage of fresh rice straw was decreased according to the increasement of harvesting area. It is suggested that application of round bale silage system to fresh rice straw with molasses or inoculant was the best treatment for improving preservation as silage, and that animal intake was enhanced by addition of inoculant to fresh rice straw.

• Journal of East-West Nursing Research
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• v.7 no.1
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• pp.32-47
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• 2002

This study is a descriptive comparative investigated one to analyze nursing education system and curriculum of Korea and China trans-culturally. 1) Education System The basic level of nursing education in Korea consisted of 65 3-year- junior college of nursing (7379 students) and 48 4-year-bachelor of nursing college (2345 students) in 2000 showing more 3-year-junior college of nursing and its students. In China, western nursing as well as Chinese nursing education system were operating. In 2000, 501 western school of middle technical nursing, 29 school of middle technical nursing of middle level education, and 89 3-year western and 24 Chinese junior college of nursing, and 42 4-year western bachelor of nursing college and 10 Chinese of high level education have been established. The presence of Chinese school of middle technical nursing system seemed to be in slower development in nursing than Korea, but that of Chinese nursing education seemed to be advanced with its national identification prior to Korea. Post graduate continuous education for RN-Diploma and RN-BSN program has been opened as in Korea. The Hosa(護士) system in school of middle technical nursing in China reflects lower level of education than Korea. But it can be a merit, other than in Korea, without nurses aids, when they are acting under supervision of nurses and led by them, and there presents a special course for promotion up to high level education. Graduate school in Korea is divided into general type opening a curriculum for MS in 1960 and as of 2000, 21 general types for majoring in nursing. The PhD course in Korea was established in 1978, and after that the PhD courses have been opening in 14 universities at present. China established master degree course in 1991 and as of 7 colleges are ongoing, and the doctoral course is now under planning, resulting slower development than Korea. 2) Education of theory and clinical practice in Korea and Chine (1) Korea's 3-year junior nursing college have 51 subjects, 49 subjects in China, which was not different. China was following education of ideology and medical. 4-year Bachelor of Science College has 92 subjects in Korea with cultural subjects and essential major studies/elective in theory education in Korea, while 63 subjects in China, showed wider selection in Korea's education. (2) Korea's 3-year and 4-year nursing colleges performed clinical practice education parallel with theoretical education for a certain period, block or theory/practice system. While China's 3-year and 4 or 5-year-colleges educated the theory first and then practice for one year in the last grade, integrating each situation of the departments and the theory. (3) Korea's oriental nursing theory in nursing education was performed in 28 colleges of 65 nationwide ones of 3-year junior nursing colleges, but only one school was educating clinical practice. In 4-year bachelor of nursing colleges, the oriental nursing theory was done in 14 among 48 investigated. And 1-4 subjects of them were doing, and 4 schools performed of clinical practice, showing more reinforced than the junior colleges. China's 3-year and 4-5-year western nursing colleges taught two subjects of Chinese medicine nursing. China's 3-year & 4-5-year College of Chinese medicine nursing, theory of Chines medicine nursing education taught eight subjects. (4) 5-year colleges of Chinese medicine western integrated nursing, theory of Chinese medicine nursing education consisted of twelve Chinese medicine nursing subjects and two of Chinese medicine western integrated nursing subjects. China was tempting a new development of a pattern of Chinese medicine nursing subjects reinforced. 3) The verification of Korean and Chinese nurse's license. The verification test of Chinese nurses license is differentiated at the level of education other than in Korea. Expire date is 2 years and a qualified test must be done to a renewal. And the continuing education hours are 72 per year, which is more enforced than Korean nurses (10 hours a year). In accordance with WTO regulations, we should prepare for opening foreign hospital, educating oriental nursing subjects. And on this, it is recommendable to settle a basic frame research to run the oriental nursing practice ongoing. 1. It is desirable to develop the oriental nursing subjects to apply its idea to the western nursing and differentiate Korean nursing. 2. It is desirable to certify oriental nurse's characters, to expand and develop the nursing areas to practice it, and to establish the oriental nursing system. 3. It is expectable to promote Korean nursing specialization to develop the oriental nursing as a professional and to create its demand.

• Journal of Animal Science and Technology
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• v.52 no.1
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• pp.29-36
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• 2010

This experiment was conducted to evaluate the influence of phase feeding methods on growth performance, carcass characteristics, pork quality, and economical efficiency in growing-finishing pigs. A total of 120 crossbred pigs ([Yorkshire ${\times}$ Landrace] ${\times}$ Duroc), average initial body weight 25.23 ${\pm}$ 2.66 kg, were allotted to 5 treatments by body weight and sex in a randomized complete block (RCB) design. Each treatment had 6 replicates with 4 pigs per pen. The treatments were 1) A (fed late weaner feed during whole phase), 2) B (fed late weaner feed in growing phase and grower feed in finishing phase), 3) C (fed grower feed during whole phase), 4) D (fed grower feed in growing phase and early finisher feed in early and late finishing phase), 5) E (fed grower feed in growing phase, early finisher feed in early finishing phase and late finisher feed in late finishing phase). Commercial diets bought from private feed company were used for this experiment. During the early growing phase (6 wks), treatments A and B showed higher growth performance than that of other treatments (P<0.05). However, growth performance tended to be similar among treatments at 10 wks after feeding and there was no difference in time of market weight (13 wks). Moreover, blood urea nitrogen (BUN) concentration was lowered in treatment E than other treatments during whole experimental period (P<0.01). Carcass characteristics and pork quality were measured when body weight of pigs reached at 110.33 ${\pm}$ 2.1 kg. Days to market weight (110 kg), quality grade, pork color, and pork pH did not show any significant difference among treatments. These results suggested that phase feeding based upon growth stages of pigs showed no detrimental effects on growth performance and the pork quality. Moreover, feed cost was saved approximately 37% by phase feeding method without delaying of days to market weight compared to general conventional feeding method (B) of swine farm in Korea.

• Applied Microscopy
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• v.30 no.4
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• pp.357-365
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• 2000

Paneth cells have been suggested to contribute to the elimination of excess metals into the intestinal lumen. The purpose of this study wat to investigate the changes of the zinc pools in rats subjected to functional loading with zinc salt by mean of both light and electron microscopical autometallography (AMG). Wistar rats 4 were administrated with zinc chloride (20 mg/kg body weight) intraperitoneally dissolved in 1 ml distilled water. The control group received 1 ml saline IP. After further one hour the animals were transcardially perfused with 0.4% sodium sulphide dissolved in 0.1 M PB fellowed by 3% glutaraldehyde solution for 10 minutes. Pieces of ileum were frozen with solid $CO_2$ and sectioned on a cryostat. The sections $(20{\mu}m)$ were autometallographically developed. Sections selected for EM were reembedded on top of a blank Epon block, from which ultrathin sections (100 nm) were cut. The ultrathin sections were double stained with uranyl acetate (30 min) and lead citrate (5 min), then examined under electron microscope. Studies of comparable sections from control and zinc loaded animals with the AMG selenium method gave quite different results. The control animals demonstrated a weakly positive staining in the cytoplasm of the Paneth cells. In the electron microscope the AMG silver grains were found to be located in the cytoplasm, while the electron dense secretary granules and other cell organelles were void of staining. Few AMG grains were located at the apical surface of the Paneth cells. In sections from zinc loaded rats, the AMG grains were seen in abundance in the lumen of the Lieberkuhn crypts at light microscopic levels. At EM levels the zinc revealing silver grains were located in the cytoplasm as in the controls, but much more AMG grains were shifted into the secretary granules. Furthermore, profound AMG grains were found in the lumen of the crypts and surrounding vessels. And a few grains were seen in the endothelium. The AMG technique demonstrated a pattern of AMG grains in the Paneth cells that strongly suggests a transport of zinc ions through these cells.

• The Korean Journal of Pharmacology
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• v.31 no.1 s.57
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• pp.39-51
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• 1995

The roles of ${\beta}-adrenoceptor$ were well known in hyperthyroidal heart, but not with ${\alpha}-adrenoceptor$. So we studied the effects of phenylephrine on membrane potential, intracellular sodium activity ($a^{i}_{Na}$), twitch force, and intracellular pH ($pH_i$) by continuous intracellular recordings with ion-selective and conventional microelectrodes in the papillary muscles of hyperthyroid guinea pig heart. ${\alpha}_1-adrenoceptor$ stimulation by phenylephrine (10^{-5}\;or\;3{\times}10^{-5}M$) produced the following changes: variable changes in action potential duration, a hyperpolarization ($1.5{\pm}0.1mM$) of the diastolic membrane potential, an increase in $a^{i}_{Na}\;(0.4{\pm}0.15mM)$, a stronger positive inotropic effect ($220{\pm}15%$), an increase in $pH_i\;(0.06{\pm}0.002\;unit)$. These changes were flocked by prazosin and atenolol. This indicated that the changes in membrane potential, $a^{i}_{Na}$ twitch force, and $pH_i$ are mediated by a stimulation of the ${\alpha}_1-adrenoceptor$. Ethylisopropylamiloride ($10^{-5}$) also blocked the increase in $a^{i}_{Na}$ and twitch force. On the other hand, strophanthidin, tetrodotoxin, $Cs^+$ or verapamil did not block the increase in $a^{i}_{Na}$ and twitch force. Thus, it was suggested that ${\alpha}_1-adrenoceptor$ stimulation increased $a^{i}_{Na}\;and\;pH_i$ by stimulation of $Na^{+}-H^{+}$ exchange, thereby allowing intracellular alkalinization and $a^{i}_{Na}$ increase. These results were very different from euthyroidal heart which showed ${\alpha}_1-adrenoceptor$-induced decrease in $a^{i}_{Na}$ and initial negative inotropic effect. From the above results, it was concluded that ${\alpha}_1-adrenoceptor$ had a important role in hyperthy-roidal heart.

• Journal of The Korean Society of Grassland and Forage Science
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• v.26 no.4
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• pp.257-266
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• 2006

Studies were carried out to evaluate the effect of liquid pig manure on the production of Japanese millet, the chemical characteristics of pasture soil and leaching water. The study was undertaken from June to September 2005. Randomized complete block design was used to allot four treatments: T1 (no fertilizer), T2 (N : 200 kg/ha, p : 150 kg/ha, K : 150 kg/ha), T3 (liquid pig manure containing 1.7% DM 200 kg N/ha) and T4 (liquid pig manure containing 7.0% DM 200 kg N/ha). Leaching water was sampled at 21 August (1st time), 9 September (2nd time) and 26 September (3rd time), 2005, respectively. No significant differences in the dry matter yield of Japanese millet was found among the four treatments, whereas the plant lengths of Japanese millet were higher in the T2, T3 and T4 than in T1 (p<0.05). Nitrogen, P and K uptake of J. millet tended to be influenced by application of chemical fertilizer or 7.0% DM liquid pig manure compared with T1 or 1.8% DM liquid pig manure. The organic matter (OM) content of soil was higher in T2, T3 and T4 than in T1. Na content was highest in T3 among the four treatments. $NO_{3^-}N\;or\;NH_{4^-}N$ content in leaching was not different among the four treatments. $SO_4$ content in leaching water sampled in 1st time was high in T4, but in T3 from 3rd time (p<0.05). Cl, Mg and Na contents were high in leaching water sampled in the 1st time from T4, whereas high in those from T3 in 2nd or 3rd time. Results show that the application of a high DM liquid pig manure is not better for producing Japanese millet and improving the properties of pasture soil than a low DM liquid pig manure. However, the contents of $SO_4$, Cl, Mg and Na in leaching water sampled in 1st time were high in a high DM liquid pig manure.