• Journal of Acupuncture Research
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• v.18 no.6
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• pp.250-263
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• 2001

Objective : I refered to oriental medical records to study on the use Ohaeng-acupuncture through compared ${\ll}$Classic on Difficulty${\gg}$ with . Methods : The original text about ${\ll}$Classic on Difficulty${\gg}$ was used ${\ll}$Nan Jing Ben YI${\gg}$, annotations were excerpted and record that were necessary for this study. The structural formula was composed together to compare ${\ll}$Classic on Difficulty${\gg}$ with . Results : ${\ll}$Classic on Difficulty${\gg}$ deals with fundamental medical theories and gives differentiation of syndromes of some diseases in the form of questions and answers. ${\ll}$The Sixty nineth Difficulty, Classic on Difficulty${\gg}$ 'Xu Ze Bu Qi Mu(虛者補其母), Shi Ze Xie Qi Zi (實者瀉其子)' that united ${\ll}$Ling Shu(靈樞) - Jing Mai(經脈篇)${\gg}$ 'Sheng Ze Xie Zhi(盛則瀉之) Xu Ze Bu Zhi(虛則補之)' with Ohaeng-xiangsheng theory is the base of the 'Bu Xie (補瀉)'. ${\ll}$The seventy fifth Difficulty, Classic on Difficulty${\gg}$ 'Xie Nan Huo (瀉南方火) Bu Bei Shui (補北方火)' that based Ohaeng-xiangke theory and the 'Qu Xue(取穴)' takes the form of the 'Bu Mu Xie Zi (補母瀉子)' in standard of internal organs which are etiologic al cause named 'Shi(實)'.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.4
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• pp.1431-1437
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• 2012

The growth of many breast tumors is stimulated by IGF-1, which activates signal transduction pathways inducing cell proliferation. $ER{\alpha}$ is important in this process. The aim of the study was to investigate relationships in vitro among inhibitory effects of luteolin on the growth of MCF-7 cells, IGF-1 pathway and $ER{\alpha}$. Our results showed that luteolin could effectively block IGF-l-stimulated MCF-7 cell proliferation in a dose- and time-dependent manner and block cell cycle progression and induce apoptosis evidenced by the flow cytometric detection of sub-G1DNA content. Luteolin markedly decreased IGF-l-dependent IGF-IR and Akt phosphorylation without affecting Erk1/2 phosphorylation. Further experiments pointed out that $ER{\alpha}$ was directly involved in IGF-l induced cell growth inhibitory effects of luteolin, which significantly decreased $ER{\alpha}$ expression. Knockdown of $ER{\alpha}$ in MCF-7 cells by an $ER{\alpha}$-specific siRNA decreased the IGF-l induced cell growth inhibitory effects of luteolin. $ER{\alpha}$ is thus a possible target of luteolin. These findings indicate that the inhibitory effect of luteolin on the growth of MCF-7 cells is via inhibiting IGF-l mediated PI3K-Akt pathway dependent of $ER{\alpha}$ expression.