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Epistatic Interaction Analysis of Two Dull Genes, wx-mq and du1, Affecting Amylose Content Using Nearly Isogenic Lines in Rice

  • Ju-Won Kang;Ji-Yoon Lee;Gi-Un Seong;Youngho Kwon;So-Myeong Lee;Dong Jin Shin;Sais-Beul Lee;Hyunnggon Mang;Dong Soo Park;Jong-Hee Lee;Jun-Hyeon Cho;Gi-Won Oh
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.267-267
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    • 2022
  • Glutinous rice is a key grain quality trait occupying an important part during rice processing in most rice growing areas. Amylose content (AC) of rice determine eating quality which is one of the major traits in rice breeding program. In this study, a gene pyramiding approach was used to introduce two dull genes, responsible for low amylose contents, for glutinous rice breeding using marker assisted selection (MAS). Two dull genes were located on chromosome 6 (wx-mq, AC: 12.7 %) and chromosome 10 (du1, AC: 10.3%), respectively. To test whether these two dull genes have an epistatic interaction, we developed an F2 population by crossing two nearly isogenic lines(NILs) harboring wx-mq and du1. Gene based marker and KASP marker were used to select NILs(NIL-nor, NIL-wxmq, NIL-du1, and NIL-wxmq/du1) from the F2 population. A two-way ANOVA revealed an epistatic interaction between the two genes in the F2 population. The mean of Amylose contents for NIL-nor, NIL-wxwq, NIL-(du1, and NIL-wxmq/du1 were 17.3%, 12.5%, 9.7%, and 7.2%, respectively. This interaction was confirmed by an analysis of NILs indicating that both genes are involved in the same genetic mechanism controlling amylose contents. This result will be useful for rice breeding related to amylose content.

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DEVELOPMENT OF THE PRE-PROCESSOR FOR CFD (CFD용 전처리장치 개발)

  • Kim, S.R.
    • Journal of computational fluids engineering
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    • v.18 no.2
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    • pp.72-77
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    • 2013
  • The Pre-processor program for CFD is being developed using wxWidgets and OpenGL libraries. This program can be run on both Windows and Linux operating systems. Undergraduate students and beginners can learn and use this very easily by menu and templates. Until now, structured mesh can be created in Cartesian or Cylindrical coordinates. This program will be used easily to make various type of meshes using templates.

Propellane 분자들의 안정성 특성에 대한 고찰

  • Sin, Chae-Su;Son, Mun-Gi
    • Proceeding of EDISON Challenge
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    • 2015.03a
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    • pp.167-170
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    • 2015
  • 이 연구에서는 다양한 유기 반응에 등장하는 propellane의 흥미로운 안정성 특성을 이해하기 위해 시뮬레이션을 통해 접근하였다. [1.1.1]propellane은 ring strain이 비교적 약한 [2.2.2]propellane보다도 안정적이다. EDISON에 업로드 되어있는 GAMESS 패키지 및 wxMacmolplt을 활용하여 이러한 현상에 대해 이론적 연구를 진행하였다. 이를 통해 실제로 보고된 이례적인 안정성을 여러 종류의 propellane 분자에 대한 시뮬레이션 결과 비교를 통해서도 확인하였으며, 분자오비탈 모양과 같은 정성적인 시뮬레이션 결과뿐만 아니라 계산된 에너지준위 간격, 진동자 강도 등의 정량적인 수치들 역시 이러한 안정성과 합치하는 결과를 보였다.

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Investigation of electrochromism thin films deposited by RF magnetron sputtering (RF 마그네트론 스퍼터링법으로 증착한 전기변색 박막의 물성평가)

  • Jo, Sang-Hyeon;Lee, Jae-Geun;Lee, Seong-Ho
    • Proceedings of the Korean Institute of Surface Engineering Conference
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    • 2015.05a
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    • pp.105-105
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    • 2015
  • 전기변색 (electrochromism) 박막은 전기화학적 산화, 환원 과정을 통해 가역적인 광학 특성의 변화를 갖는 현상을 말하며, 이를 이용한 전기변색 소자(electrochromic device)는 전력 소모가 적고 변색 효율이 크다는 장점으로 인해 Smart window, display, mirror 등에 응용 될 수 있다. 본 연구에서는 대표적인 전기 변색 물질인 WxOy, NixOy 전기 변색 재료의 Sputtering법으로 증착한 박막의 특성에 대하여 평가하였다.

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Design of an One-Chip Controller for an Electronic Dispenser (전자 디스펜서용 단일 칩 제어기 설계)

  • Kim, Tae-Sang;Won, Young-Wook;Kim, Jeong-Beom
    • Journal of IKEEE
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    • v.9 no.2 s.17
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    • pp.101-107
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    • 2005
  • This paper presents an one-chip controller for an electronic dispenser. The electronic dispenser is composed of electronic part and mechanical part. The electronic part is consisted of input keypad, micro-controller, display module, and pump module. In this paper we designed micro-controller for the electronic part. The micro-controller controls display module and pump module. The display module is composed by LCD device, and the pump module is composed by motor device . The micro-controller for an electronic dispenser is designed by VHDL. We used WX12864AP1 for the LCD device and SPS20 for the stepping motor. Also, the micro-controller is designed by Altera Quartus tool and verified with Agent 2000 Design-kit using APEX20K Device. In this paper, we present possibility to adopt of the biomedical device through the one-chip controller for the electronic dispenser.

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The Roles of Hydroxyl Substituents in Tyrosinase Inhibitory Activation of Flavone Analogues (Flavone 유도체들의 Tyrosinase 저해활성화 반응에서 Hydroxyl 치환기들의 역할)

  • Park, Joon-Ho;Sung, Nack-Do
    • Journal of Applied Biological Chemistry
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    • v.54 no.1
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    • pp.56-62
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    • 2011
  • Molecular docking of polyhydroxy substituted flavone analogues (1-25) as substrate molecules to the active site of tyrosinase (PDB ID: Deoxy-form (2ZMX) & Oxy-form (1WX2)) and Free-Wilson analysis were studied to understand the roles of hydroxyl substituents ($R_1-R_9$) in substrate molecules for the tyrosinase inhibitory activation. It is founded from Free-Wilson analysis that the $R_1$=hydroxyl among $R_1-R_9$ substituents had the strongest influence on the tyrosinase inhibitory activity. H-bonds between the hydroxyl substituents of substrate molecules and amino acid residues in the active site of tyrosinase were contributed to make a stable substrate-receptor complex compound. Particularly, it is proposed from the findings that the noncompetitive inhibitory activation would take place via H-bonding between peroxide oxygen (Per404) atom in the active site of tyrosinase and the hydroxyl substituents in substrate molecule.

Design and Prototype Implementation of Geo-browser Linked to Open Source-based DBMS and Middleware (공개소스 DBMS 미들웨어 연동 공간정보 브라우저 설계 및 프로토타입 구현)

  • Park, Yong-Jae;Lee, Ki-Won
    • Korean Journal of Remote Sensing
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    • v.26 no.2
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    • pp.99-108
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    • 2010
  • According to the recent trend on advancement of web computing technologies and diversification of spatial data types to be processed, designing and implementing of web-based applications using open sources has been regarded as one of important users-needs. In this study, a kind of geo-browser model composed of client-middleware-DBMS stack is suggested, and the prototype implementation are performed. Especially, modularization of user interfaces is contributed to increase both applicability for a certain target system and accessibility for web users. In middleware, it has functions to decrease erroneous factors on spatial data registration processes, and provides spatial data the form of OGC WxS standards. It is thought that this system is helpful to utilize as basic architecture and the related implementation model for web-based geo-spatial services and their applications.

Screening Wheat Germplasm for high Amylose Content

  • Kyeong-Hoon Kim;Jinhee Park;Mina Kim;Kyeong-Min Kim;Yurim Kim;Go Eun Lee;Chon-Sik Kang;Jiyoung Shon;Jong-Min Ko
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.300-300
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    • 2022
  • Wheat (Triticum spp.) is an important source of food worldwide and the focus of considerable efforts to identify new combinations of genetic diversity for crop improvement. In particular, wheat starch composition is a major target for changes that could benefit human health. Starches with increased levels of amylose are of interest because of the correlation between high amylose content and elevated levels of resistant starch, which has been shown to have beneficial effects on health for combating obesity and diabetes. In this study, high amylose wheat germplasms from other countries were collected and cultivated in Korea, and then the content of amylose was evaluated, we examined amylose content in 614 wheat germplasm. Furthermore, amylose content was validated using several milling processes such as roller, hammer, and grinding mill. As a result, the amylose content distribution was divided into five groups. The range of the amylose levels in whole wheat flour was 18.3% to 29.6%. In addition, the mutant lines were screened for high amylose, and two mutant lines (WX-1046 and WX-1074) exhibited a comparable amylose content to Keumkang whole wheat (19.6%). It has been established that high amylose indicated SS IIa null and necessitate GBSS. Based on these findings, it may be helpful to develop high amylose wheat germplasm and production techniques, particularly in Korea.

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3D-QSAR Analysis and Molecular Docking of Thiosemicarbazone Analogues as a Potent Tyrosinase Inhibitor

  • Park, Joon-Ho;Sung, Nack-Do
    • Bulletin of the Korean Chemical Society
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    • v.32 no.4
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    • pp.1241-1248
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    • 2011
  • Three dimensional quantitative structure-activity relationships (3D-QSARs) between new thiosemicarbazone analogues (1-31) as a substrate molecule and their inhibitory activity against tyrosinase as a receptor were performed and discussed quantitatively using CoMFA (comparative molecular field analysis) and CoMSIA (comparative molecular similarity indices analysis) methods. According to the optimized CoMSIA 2 model obtained from the above procedure, inhibitory activities were mainly dependent upon H-bond acceptor favored field (36.5%) of substrate molecules. The optimized CoMSIA 2 model, with the sensitivity of the perturbation and the prediction, produced by a progressive scrambling analysis was not dependent on chance correlation. From molecular docking studies, it is supposed that the inhibitory activation of the substrate molecules against tyrosinase (PDB code: 1WX2) would not take place via uncompetitive inhibition forming a chelate between copper atoms in the active site of tyrosinase and thiosemicarbazone moieties of the substrate molecules, but via competitive inhibition based on H-bonding.

Design of an One-Chip Controller for an Electronic Dispenser (전자 디스펜서용 단일칩 제어기 설계)

  • Won, Young-Uk;Kim, Jeong-Beom
    • Proceedings of the KIEE Conference
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    • 2005.05a
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    • pp.137-140
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    • 2005
  • The electronic dispenser is composed of electronic part and mechanical part. Electronic part is consisted of input keypad, micro-controller, display module, and pump module. In this paper we designed micro-controller for electronic part. The micro-controller controls display module and pump module. The display module is composed by LCD device, and the pump module is composed by motor device. The micro-controller for an electronic dispenser is designed by VHDL. We used WX12864APl for the LCD device and SPS20 for the stepping motor. Also, the micro-controller is designed by Altera Quartus tool and verified with Agent 2000 Design-kit using APEX20K Device. In this paper, we present possibility to adopt of biotechnology field through designing of one-chip controller for an electronic dispenser.

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