• Biomolecules & Therapeutics
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• v.30 no.4
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• pp.348-359
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• 2022

Gastric adenocarcinoma is among the top causes of cancer-related death and is one of the most commonly diagnosed carcinomas worldwide. Benzyl isothiocyanate (BITC) has been reported to inhibit the gastric cancer metastasis. In our previous study, BITC induced apoptosis in AGS cells. The purpose of the present study was to investigate the effect of BITC on autophagy mechanism in AGS cells. First, the AGS cells were treated with 5, 10, or 15 μM BITC for 24 h, followed by an analysis of the autophagy mechanism. The expression level of autophagy proteins involved in different steps of autophagy, such as LC3B, p62/SQSTM1, Atg5-Atg12, Beclin1, p-mTOR/mTOR ratio, and class III PI3K was measured in the BITC-treated cells. Lysosomal function was investigated using cathepsin activity and Bafilomycin A1, an autophagy degradation stage inhibitor. Methods including qPCR, western blotting, and immunocytochemistry were employed to detect the protein expression levels. Acridine orange staining and omnicathepsin assay were conducted to analyze the lysosomal function. siRNA transfection was performed to knock down the LC3B gene. BITC reduced the level of autophagy protein such as Beclin 1, class III PI3K, and Atg5-Atg12. BITC also induced lysosomal dysfunction which was shown as reducing cathepsin activity, protein level of cathepsin, and enlargement of acidic vesicle. Overall, the results showed that the BITC-induced AGS cell death mechanism also comprises the inhibition of the cytoprotective autophagy at both initiation and degradation steps.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.20 no.2
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• pp.161-166
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• 2009

In this paper, we propose the internal mobile antenna for TDMB and KPCS. The proposed antenna is made of different dielectric substrate and it has small size ($45{\times}8{\times}8\;mm$, about 2.8 cc) for mobile device. TDMB antenna is designed spiral structure that makes maximum current for each cell and KPCS antenna is PIFA that is usually used for internal antenna. In order to compensate length of resonance TDMB antenna has a large inductor above 100 nH. In this case, the inductor isolate KPCS signal at TDMB by cutting high frequency. Also the antenna has good isolation because TDMB radiator is parasitic element in KPCS band. We simulated the antenna by using CST microwave studio and measured performance of the antenna in anechoic chamber Proposed antenna has $-6{\sim}-14\;dBi$ gain for TDMB and $-3.5{\sim}-5\;dBi$ gain for KPCS.

• Clinical and Experimental Reproductive Medicine
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• v.27 no.2
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• pp.201-209
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• 2000

Objective: This study has been carried out to evaluation the effect of fertilization promoting peptide (FPP) on the kinematic parameters, capacitation and acrosome reaction of the frozen-thawed human spermatozoa. Methods: After FPP treatment, we examined kinematic parameters, capacitation and acrosome reaction, using the methods of computer-aided sperm analysis (CASA) and chlortetracycline (CTC) fluorescence analysis. Results: We have obtained the evidence that FPP can promote the capacitation and inhibit the spontaneous acrosome reaction of frozen-thawed human spermatozoa in vitro. FPP ($25{\sim}100$ nM) induced a significant increase in the proportion of B-pattem capacitated spermatozoa, and a significant decrease in the proportion of F-pattem uncapacitated ones without significant stimulation of acrosomal exocytosis. In the kinematic parameters treatment, FPP treated groups maint3ined higher LIN, BCF and STR than those of control. The VAP, VSL, VCL and ALH were not different. Therefore it is suggested that FPP in human seminal plasma may play a positive role in promoting human sperm function.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.18 no.4 s.119
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• pp.358-363
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• 2007

The multi-band and wide-band antenna for a portable broadcasting terminal is proposed. The proposed antenna consists of two radiators with a parallel structure. The antenna has an enough wide impedance bandwidth for the DVB-H(Digital Video Broadcasting-Handheld) service band since two radiators have adjacent resonance frequencies and operates in the DAB(Digital Audio Broadcasting) service band using the third harmonic of the radiator 1. The fabricated antenna has VSWR characteristics of less than 2:1 in the frequency band $470{\sim}740\;MHz$ for DVB-H and $1,450{\sim}1,480\;MHz$ for DAB. The measured peak gain of the antenna is $1.97{\sim}4.10\;dBi$ in the DVB-H band and $1.98{\sim}2.04\;dBi$ in the DAB band.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.19 no.9
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• pp.978-984
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• 2008

In this paper, we propose a RFID metal tag antenna with a minimum by size of a metal surface to attach. This proposed tag antenna is a patch antenna which is able to stick on metal surface and designed for very slim structure ($119{\times}30{\times}1.6$ mm) antenna that is matched to a chip impedance. This has a loop coupling feeding and consists of a inner radiator and a outer radiator. The outer radiator activates the current to concentrate on the inner radiator regardless of metal size to attach. Also the tag antenna is designed by CST microwave tool and the performance is measured in the anechoic chamber. The optimum antenna has 3.77 % of the matching bandwidth($S_{11}<-10$ dB). The readable range of the tag antenna is about 2.9 m on metal(max. size $700{\times}700$ mm) and 5.5 m in free space according to the measurement results.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.19 no.7
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• pp.719-725
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• 2008

In this paper, we have proposed a printed monopole antenna using parasitic elements. The broadband characteristics of the antenna is obtained by using the printed monopole and parasitic elements. To confirm the broadband characteristics of the antenna, of have designed and fabricated the proposed antenna. The return loss and radiation patterns are measured in the frequency range of$0.8{\sim}1.2\;GHz$. The measured results show that the proposed antenna has return loss less than -10 dB in the operating frequency band and the radiation pattern is the dipole-like patterns. The antenna gain varies from 1.7 to 4.6 dBi in the operating frequency band. Thus, the proposed antenna can be used for the broadband repeater antenna $0.806{\sim}0.960\;GHz$(TRS-800, CDMA and GSM-900).

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.18 no.2 s.117
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• pp.227-232
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• 2007

In this paper, we propose Triple-band antenna, which is practicable for the transmission and the reception of the frequency band used in mobile broadcast and mobile internet. We choose T-DMB(Band-III), DAB(L-Band) for a mobile broadcast and Wibro for a mobile internet. The size of proposal antenna measures $10{\times}74{\times}0.6[mm^3]$. Although being defective in that T-DMB bandwidth is rather narrow, the antenna, considering the reception environment, has an advantage of a realistic possibility of reception in 8 channel($180{\sim}186$ MHz)and 12 channel($206{\sim}210$ MHz) of T-DMB band, being given broadcast services. Also It has gains similar characteristics of ideal dipole antenna in DAB and Wibro band.

• Clinical and Experimental Pediatrics
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• v.50 no.11
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• pp.1067-1071
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• 2007

Purpose : The aim of this study was to determine whether improved survival of extremely low birth weight infants (ELBWI) was associated with decreased neurodevelopmental disability later in life, and also to identify the factors influencing this disability. Methods : ELBWI admitted to the neonatal intensive care unit of Samsung Medical Center, survived, and followed up until the corrected age of 18 months were enrolled. They were divided into two groups according to admission time: period I (1994-1999, n=36) and period II (2000-2004, n=98). Clinical data were collected retrospectively from the medical records. Results : Survival rates increased from 60.0% to 74.7%, cerebral palsy rates decreased from 22.2% to 8.2% and catch-up growth rate increased from 25.0% to 51.0% during period I and II. Despite less gestational age and birth weight, ELBWI during period II had less periventricular leukomalacia (PVL), sepsis and bronchopulmonary dysplasia compared to period I. The highest risk factors for cerebral palsy were intraventricular hemorrhage (IVH) (${\geq}$Grade III), failure of catch-up growth and PVL. Conclusion : In summary, improved viability was associated with decreased neurodevelopmental disability in ELBWI. Improved neonatal care with resultant decrease in PVL and IVH, and better nutritional support seem to be primarily responsible for this improved outcome.

• Journal of Veterinary Clinics
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• v.24 no.4
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• pp.667-672
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• 2007

This report describes the use of conventional computed tomography(CT) for the diagnosis of head trauma in three canine patients. According to physical and neurologic examinations, survey radiography and computed tomography, these patients were diagnosed as traumatic brain injury. Especially, CT is the imaging modality of first choice for head trauma patients. It provides rapid acquisition of images, superior bone detail, and better visualization of acute hemorrhage than magnetic resonance imaging. It is also less expensive and more readily available. Pre-contrast computed tomography was used to image the head. Then, post-contrast CT was performed using the same technique. The Modified Glasgow Coma Scale(MGCS) score was used to predict their probability of survival rate after head trauma in these dogs. Computed tomogram showed fluid filled tympanic bulla, fracture of the left temporal bone and cerebral parenchymal hemorrhage with post contrast ring enhancement. However, in one case, computed tomographic examination didn't delineate cerebellar parenchymal hemorrhage, which was found at postmortem examination. Treatments for patients placed in intensive care were focused to maintain cerebral perfusion pressure and to normalize intracranial pressure. In these cases, diagnostic computed tomography was a useful procedure. It revealed accurate location of the hemorrhage lesion.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.18 no.1 s.116
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• pp.44-52
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• 2007

In this paper, An active antenna of T-DMB was developed to equip to handhold gadgets by using LNA and a parasitic element allowing to miniature. The size of the fabricated active antenna is $80{\times}6{\times}0.4\;mm$ and FR4 is used for the substrate. The size of the proposed antenna is reduced by 38.8% at the operating frequency compared to one without a parasitic element, and a short stub. The proposed antenna shows improved performance at the measurement especially in the ratio of S/N compared with conventional monopole of 300 mm. The proposed antenna is well able to adapt into handhold gadgets for receiving T-DMB.