• 한국응용약물학회:학술대회논문집
• /
• 한국응용약물학회 1998년도 Proceedings of UNESCO-internetwork Cooperative Regional Seminar and Workshop on Bioassay Guided Isolation of Bioactive Substances from Natural Products and Microbial Products
• /
• pp.172-173
• /
• 1998

A research collection of Pseudomons solanacearum bacteria, a pathogen causing ‘bacteria wilt’ disease of more than 265 plant species, represented for northern provinces of Vietnam has recently been established and was saved for examination of antifungal activity in their culture fluids. All strains used in this work have been isolated from infected tomato, potato, and groundnut collected from production fields and they express different levels of virulence on their host plants. Cell-free culture fluids of these strains were tested for antifungal activity (to inhibit growth of mycelium and to destroy germination tube of fungal spores) on a number of fungi that either infect or associate with vegetable crops of Solanaceae family (tomato, potato, pepers...), fruit plants (banana), and even well-known by Vietnamese traditional medicine herbal plants belonging to Trifoliatus, Schefflera, Homalomena and Panax genera (Araliaceae family) of which roots are used as a resource of the herbal material. The antifungal activity was found in nearly all strains tested. Result of study on chitin, CMC, tween 80 and casein degradation abilities of the latter suggested that antifungal activity of positively-found strains may be due to their ability of extracelluar chitinase's excretion that destroy fungal cell wall.

• The Korean Journal of Pain
• /
• 제1권1호
• /
• pp.98-102
• /
• 1988

In order to control the pain after hemorrhoidectomy and anal fistulectomy, 6 mg of 0.5% hyperbaric tetracaine without(control, group I) or wilt 0.3 mg(group II) or 0.5 mg (group III) of 0.1% morphine was injected with a 22 gauge spinal needle into the subarachnoid space through L 3-4 interspace of patients in lateral position. About 30 minutes in Fowler' sposition after injection, operation was performed in lithotomy position. All the patients who ha4 morphine showed remarkable relief of postoperative pain for an average of 27 hours. However, the dosage(0.3 or 0.5 mg) of morphine administered did not affect the duration of pain relief. Blood pressure, pulse rate and pupil size were unchanged in all patients. Dysuria after block developed for on average of 5,6, 13.2 and 14.6 hours in group I, II and III respectively. Most of these cases required urethral catheterization. Minor complications such as nausea, vomiting, itching, fever, burning sensation and paresthesia were observed 16.7, 20 and 20% of cases in group I, II and III respectively; however, no treatment was required.

• 지구물리와물리탐사
• /
• 제7권1호
• /
• pp.51-55
• /
• 2004

This paper presents a fast and stable imaging scheme using the localized nonlinear (LN) approximation of integral equation (IE) solutions for inverting electromagnetic data obtained in a crosswell survey. The medium is assumed to be cylindrically symmetric about a source borehole, and to maintain the symmetry a vertical magnetic dipole is used as a source. To find an optimum balance between data fitting and smoothness constraint, we introduce an automatic selection scheme for a Lagrange multiplier, which is sought at each iteration with a least misfit criterion. In this selection scheme, the IE algorithm is quite attractive for saving computing time because Green's functions, whose calculation is a most time-consuming part in IE methods, are repeatedly re-usable throughout the inversion process. The inversion scheme using the LN approximation has been tested to show its stability and efficiency, using both synthetic and field data. The inverted image derived from the field data, collected in a pilot experiment of water-flood monitoring in an oil field, is successfully compared with that derived by a 2.5-dimensional inversion scheme.

• Mycobiology
• /
• 제30권2호
• /
• pp.102-104
• /
• 2002

In July 2001, a destructive stem rot of tatarian aster(Aster taturicus) was occurred sporadically in exhibition farm of Gyeongsangnam-do Agricultural Research and Extension Services, in Hamyang, Korea. The fungus also caused collar and crown rot and systemic wilt or blight of whole plant. White mycelium spread over stems and petioles of infected plants and sclerotia formed on the old lesions and near the soil surface. The fungus showed maximum mycelial growth was obtained around $30^{\circ}C$ but did not grow below $5^{\circ}C$ or above $45^{\circ}C$. The mycelial width ranges $4.2{\sim}10.4{\mu}m$. and the color is white, usually many narrow mycelial stand grow in the aerial mycelium and formed clamp connection. Numerous sclerotia were formed in artificial media like PDA at $30^{\circ}C$. The shape of sclerotia were sphere and $1.0{\sim}3.2{\mu}m$ in diameter. The fungus was isolated repeatedly from the infected, tissues and confirmed its pathogenecity to aster and identified as Sclerotium rolfsii. This is the first report that Sclerotium rolfsii causes stem rot of tatarian aster in Korea.

• Journal of Microbiology and Biotechnology
• /
• 제22권5호
• /
• pp.674-683
• /
• 2012

A new strain, SD12, was isolated from tannery waste polluted soil and identified as Pseudomonas aeruginosa on the basis of phenotypic traits and by comparison of 16S rRNA sequences. This bacterium exhibited broad-spectrum antagonistic activity against phytopathogenic fungi. The strain produced phosphatases, cellulases, proteases, pectinases, and HCN and also retained its ability to produce hydroxamate-type siderophore. A bioactive metabolite was isolated from P. aeruginosa SD12 and was characterized as 1-hydroxyphenazine ((1-OH-PHZ) by nuclear magnetic resonance (NMR) spectral analysis. The strain was used as a biocontrol agent against root rot and wilt disease of pyrethrum caused by Rhizoctonia solani. The stain is also reported to increase the growth and biomass of Plantago ovata. The purified compound, 1-hydroxyphenazine, also showed broad-spectrum antagonistic activity towards a range of phytopathogenic fungi, which is the first report of its kind.

• 한국과학교육학회지
• /
• 제12권2호
• /
• pp.81-95
• /
• 1992

The purpose of the study is to specify the evaluative objectives of science process skills and to serve as an evaluative criterion for the development of university competency test. The followings are the results of this study. (l) Five steps as a teaching and learning model of science are suggested for the improvement of science inquiry skills. (2) Three dimensional taxomony was presented to evaluate the science process skills of university competency test. (3) Sixteen behavioral elements were selected from the science process model and defined operationally. (4) 146 evaluative objectives were specified according to each behavioral element based on the results presented above, the science inquiry model and the evaluative objectives wilt be contributed to teaching and learning strategies for the improvement of science process skills including basic concepts and contents, and problematic situation in science.

• 미생물학회지
• /
• 제54권2호
• /
• pp.136-139
• /
• 2018

풋마름병 균에 대해 강한 저해활성을 갖는 활성물질 Compound S와 conversion product인 compound S'이 Bacillus subtilis JW-1 배양액에서 일련의 크로마토그래피 방법으로 분리 정제되었고, $^1H$ NMR, $^{13}C$ NMR, $^1H-^1H$ COSY와 HMBC 등의 spectra 분석에 의해 구조가 alanyl-L-${\beta}$-(2,3-epoxycyclohexyl-4-one)alanine와 alanyl-L-${\beta}$-(2,3-dihydroxycyclohexyl-4-one) alanine로 각각 동정되었다. Compound S는 $G^+$, $G^-$ bacteria, yeast와 Candida albicans 등에 대해 광범위한 antimicrobial activity를 나타내며, conversion product의 활성 상실을 통해 intact oxirane ring이 Compound S의 활성에 필수적임이 밝혀졌다.

• 한국작물학회지
• /
• 제48권5호
• /
• pp.388-391
• /
• 2003

Tobacco mosaic virus (TMV), TMV-common strain, causes severe damage at growth, yield and leaf quality on flue-cured tobacco in Korea. Controlling TMV outbreak with cultivating practices and chemicals are limited, thus, the best way would be developing TMV resistant variety. A new flue-cured hybrid, KF118, was developed from a cross of maternal parent MSTC175 and TC853 at the Agro-tech. Research Group, KT＆G Central Research Institute. KF118 exhibited more vigorous growth characteristics in early stage than that of NC82, standard variety in Korea, while other characters were favorable to those of NC82, It is highly resistant to TMV and bacterial wilt(Ral-stonia solanacerum), and black shank(phytophthora parasitica var. nicotianae) is comparable to that of NC82. It should adapt well to the flue-cured production area, and can reduce premature flowering under unfavorable weather conditions. Yield of KF118 is 1 to 2% higher, and leaf quality is 4% higher than NC82. Fl hybrid, KF118, met acceptable standards for chemical and physical characteristics of cured leaf, and ranked high in good smoking taste by panel members in KT＆G.

• 한국방재학회:학술대회논문집
• /
• 한국방재학회 2008년도 정기총회 및 학술발표대회
• /
• pp.767-770
• /
• 2008

우리나라의 해안방재림의 조성실태를 파악하여 미래지향적인 관리 및 조성방안을 강구하기 위해 연구를 진행한 결과, 전국에 기 조성되어 있는 해안방재림의 면적은 약 1,479ha로 나타났다. 따라서 기존에 조성되어 있는 약 1,479ha에 대한 간벌지침 등을 마련하여 방재기능이 저하된 빈약한 임분에 대한 대비책을 마련해야 할 것이다. 또한 일부 지역에서는 소나무 재선충병과 해안침식의 위협을 받고 있으므로 이에 대한 적극적인 대비책을 마련해야 할 것이다.

• 한국연초학회지
• /
• 제16권1호
• /
• pp.69-75
• /
• 1994

A new flue - cured tobacco variety KF113 was developed by Nicotiana africana method from a cross of NC82 and Coker 347 at the Suwon Experiment Station. It was tested in the 0fficial Variety Test in 1991-1992 and the flue - cured Regional Farm Test in 1992. KFl13 flowers 4 days later than WC82 (standard variety in Korea) and its harvestable leaves are 2 more than those of NC82. The leaf type and shape of KFl13 resembles Coker 347. It has high resistance to bacterial wilt (Pseudomonas solanacearum) and black shank (Phytophthora parasitica var. nicotianae), and is susceptible to tobacco mosaic virus. It should adapt well to the flue - cured production area and can reduce premature flowering under unfavorable weather conditions. Yield of KFl13 is 5% higher than that of NC82, and nearly equal in value per kg compare with NC82. This variety met acceptable standards for chemical and physical characteristics of cured leaf and for smoking taste evaluated by panel members in Korea Ginseng & Tobacco Research Institute.