• 한국균학회소식:학술대회논문집
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• 한국균학회 2014년도 춘계학술대회 및 임시총회
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• pp.30-30
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• 2014

We isolated various yeasts from wild flowers in main islands, Jejudo, Ulleungdo and Yokjido of Korea and their yeasts were identified by comparison of their PCR-amplified D1/D2 regions of 26S rDNA using the BLAST database. Thirty two yeast strains of fourteen species were isolated from wild flowers of Jejudo. Forty eight yeast strains of twenty two species were isolated and identified from wild flowers of Ulleungdo, Korea. Sixty yeast strains belonged to twenty five species were isolated identified from wild flowers of Yokjido in Tongyeong, Korea. Only Metschnikowia reukaufii was overlapped from the three different islands areas. Two species overlapped from Jejudo and Ulleungdo: Pichia guilliermondii, Metschnikowia reukaufii. Seven species were overlapped from Ulleungdo and Yokjido: Cryptococcus albidus, Cryptococcus laurentii, Metschnikowia reukafii, Pichia scolyti, Rhodotorula glutinis, Rhodotorula graminis and Rhodotorula mucilaginosa. Four species were overlapped from Jejudo and Yokjido: Candida sp. Cryptococcus aureus, Metschnikowia reukafii and Pseudozyma sp.

• 한국균학회지
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• 제41권2호
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• pp.74-80
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• 2013

국내 해안 근접지역과 내륙 일부 지역에 서식하고 있는 야생화들로부터 분리, 동정한 효모들 중에서 국내 미기록 효모들을 선별하여 이들의 미생물학적 특성들을 조사하였다. 동해시의 야생화들에서 Candida silvae와 Cryptococcus terrestris, 안동시 부근의 야생화에서는 Auriculibuller fuscus의 국내에서 보고되지 않은 효모들을 분리하였다. 충남 내륙지역인 금산군에서는 Candida quercitrusa, Hanseniaspora opuntiae등의 미기록 효모들을 분리하였다. 또한 서해안 지역의 대천시에서는 Hannaella zeae, 태안시의 야생화에서는 Ca-ndida saopaulonensis, Sporobolomyces carnicolor, sporobolomyces phaffii등이 미기록 효모들로 분리, 선별하였고 예산군에서는 Candida oleophila와 Jaminaea angkoriensis 미기록 효모들을 분리하였다. 우리나라 서남부지역인 광주시 야생화에서는 Hanseniaspora vineae, 최남단인 완도군에서는 Pseudozyma hubeiensis, Pichia mexicana 등 모두 8곳에서 14종의 미기록 효모들을 분리하였다. 이들 미기록 효모들의 형태적, 배양학적 성질들이 조사한 결과 유포자 효모가 3종, 무포자 효모가 11종이었고 위균사 형성 효모가 7종이었으며 각기 다른 배양학적 특성을 보였다.

• 한국환경농학회지
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• 제34권3호
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• pp.238-243
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• 2015

BACKGROUND: Yeasts associated with fleabane flowers were identified using isolation methods previously applied in yeast biotechnology. A culture-based approach was required for isolation of many yeast strains associated with fleabane. METHODS AND RESULTS: We spread homogenized fleabane flowers onto GPY medium containing chloramphenicol, streptomycin, Triton X-100, and L-sorbose. We isolated 79 yeast strains from the flowers of wild fleabane, and identified the yeasts via phylogenetic analysis of isolates from agar plates. The yeast species included 39 isolates of Aureobasidium pullulans, 17 of the genus Candida, 14 of the genus Rhodosporidium, 6 of the genus Cryptococcus, and 3 of the genus Rhodotorula. CONCLUSION: Yeast isolates associated with fleabane flowers included A. pullulans (39 isolates) and other yeast species (40 isolates). Such yeast isolates may have biotechnological potential.

• 한국의상디자인학회지
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• 제10권2호
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• pp.1-10
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• 2008

Yellow, one of the five direction colors, is a prevailing color in artificial dyeing. The color has implied and symbolized wealth and power since ancient times. Yellow has been extracted mainly from flowers. Shapes, colors and scents of flowers not only have enriched emotional mentality, but have also been used for medicinal herbs, and dyestuff since the very old days. Coloring matter from flowers is very beautiful, and it has been used for dyeing textiles. Textile dyeing have formed a color culture and developed a dyeing culture since ancient times. Flowers include a variety of color matters, and can be easily obtained around us. Therefore, flowers have been a widespread natural dyestuff. It is well known that beautiful colors can be extracted from flowers, which are eco-friendly and non-polluting. In addition, flowers are easily provided. In this study, yellow wild flowers were selected as subjects. Seven flowers with color matters distiguishable through the naked eye were used: Korean forsythia, golden-wave, Mongolian dandelion, sunflower, conflower, chelidonium and chrysanthemum. Coloring matters were extracted from the seven flowers, which have been used to create a variety of colors through various dyestuffs; and the colors have been analyzed and presented. Dyeing and coloring matters were tested and analyzed, and several types of dyestuff had also been scientifically measured after treatment.

• 한국자원식물학회지
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• 제12권1호
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• pp.43-49
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• 1999

전라북도 지역내에 분포하고 있는 야생초목화를 개발하기 위한 자원식물을 조사하였다. 본 조사결과 봄철에 꽃피는 식물은 131종류(전체의 34.7%), 여름에는 219종류(58.1%), 가을에 25종류(6.6%) 그리고 겨울에는 2종류(0.5%)이었고, 봄부터 겨울까지 피는 꽃의 색깔은 모두 26종류로 다양했으며 가장 많은 꽃색은 백색으로 전체의 28.9%, 그다음은 황색 20.7%, 자주색 16.4%, 홍자색 7.9%의 순으로 나타났다. 시군지역별로 많이 심고있는 야생화는 애기똥풀, 원추리, 붓꽃, 산철쭉, 진달래, 할미꽃, 구절초, 민들레, 산국, 맥문동, 석산 등을 들 수 있다. 야생하고 있는 식물을 채취하여 그대로 시판하고 있는 식물은 26종류로 조사되었는데 보춘화, 원추리, 석산, 구절초가 그 대표적인 예에 속한다. 우리나라 야생화를 외국에 반출당하지 않고 개량하여 우수상품으로 만들 대상으로는 총 343종이며 국화과 식물 41종류, 장미과 25종류, 백합과 20종류 그리고 미나리아재비과 18종류의 순으로 조사되었다. 앞으로 보호해야 할 대상으로는 창포, 광릉요강 꽃, 개불알꽃, 새우난초, 금낭화, 호랑가시나무, 노각나무, 송악, 미선나무, 산개나리, 자주꽃방망이 등이다. 이들은 식물수가 격감되어서 희귀상태이거나 멸종위기 또는 학술상 중요하기 때문이다. 전북지역에는 식물분포상 남방인자와 북방인자가 섞여 분포하고 있을 뿐만 아니라 풍부하고 다양한 야생화 자원식물이 존재하고 있으므로 이의개발을 위한 기초자료를 마련하였다.

• 한국균학회지
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• 제40권3호
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• pp.141-144
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• 2012

자연환경에서 분포하고 있는 효모의 mycoflora를 확인하고 궁극적으로 이들을 고부가가치의 산업에 응용하기 위한 연구의 일환으로 대전과 인근 충남지역 일부의 야생화들을 채집하여 효모들을 분리, 동정하였다. 대전지역 야생화 42점에서 32종의 효모들을 54균주 분리하였고 이들 중 Cryptococcus 속균과 Phodotonula 속균 등이 우점균이었으며 Metschnikowia 속균과 같이 희귀 효모들을 20여종 분리하였다. 대전 인근의 충남 금산군과 논산군 일부지역의 야생화 28점에서 모두 18종의 효모들을 26균주 분리하였다. 이들 균 중에서 Candida 속균과 Pseudozyma 속균 등이 우점균으로 분리되었다.

• Mycobiology
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• 제42권2호
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• pp.198-202
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• 2014

Two new yeast records, Cryptococcus adeliensis YJ19-2 and Cryptococcus uzbekistanensis YJ10-4 were screened from 60 yeasts strains that were isolated and identified from wild flowers in Yokjido, Gyeongsangnam-do, Korea. The morphological and cultural characteristics of the newly recorded yeasts and the physiological functionalities of the supernatants and cell-free extracts obtained from their cultures were investigated. The two newly recorded yeasts did not form ascospores and pseudomycelia. They also grew well in yeast extract-peptone-dextrose broth. C. uzbekistanensis YJ10-4 grew in a vitamin-free medium and was also tolerant to sugar and salt. Antihypertensive angiotensin I-converting enzyme inhibitory activity of the supernatant from C. adeliensis YJ19-2 was high (71.8%) and its cell-free extract also showed very high (81.2%) antidiabetic $\acute{a}$-glucosidase inhibitory activity.

• 농업과학연구
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• 제37권3호
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• pp.383-386
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• 2010

This experiment was conducted to investigate the quality of wild flowers and mixed wild flower silages. Silage materials employed ochardgrass and mixed wild flower (orchardgrass, white clover, Achillea sibirica Ledeb., Coreopsistinctoria Nutt., Rudbeckiabicolor Nutt., Centaureacyanus L.). Six kilograms of silage materials cut from each treatment were divided into 3 containers, packed by vacuum, and fermented for 40 days. The orchardgrass silage showed significantly higher contents of crude protein than that of mixed wild flower silage. But the neutral detergent fiber and acid detergent fiber contents of orchardgrass silage were significantly lower than those of mixed wild flower silage. pH and butyric acid of orchardgrass silage were significantly lower than those of mixed wild flower silage, and significantly higher in lactic acid contents. It appears that water soluble carbohydrates needs to be supplemented, being grounded on the results above, into wild flower materials both with high fiber materials and with low substances which need lactic acid fermentation.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2014년도 추계학술대회 및 정기총회
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• pp.44-44
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• 2014

Yeasts were isolated from wild flowers of some islands and mountains such as Jeju-do, Ulleungdo, Yokjido, Seonyudo and Gyejoksan, Oseosan, Beakamsan and Deogyusan in Korea and were identified by comparison of nucleotide sequences for PCR-amplified D1/D2 region of 26S rDNA or internal transcribed pacer(ITS) 1 and 2 including 5.8S rDNA using BLAST. Seventy two yeast strains of two hundred eighty nine species were isolated from wild flowers in islands and mountains, Korea. Among them, Cryptococcus species were isolated the most dominantly, and Metschnikowia reukaufii were also isolated thirty species, 10.3% of total strains. Twenty-three species including Cryptococcus aureus were overlapped between yeast strains of the islands and mountains. Some physiological functionality of the culture broth and cell-free extracts from two hundred eighty nine yeast strains were determined. The supernatant of Candida sp. 78-J-2 showed antioxidant activity of 22.5%, and supernatant of Metschnilowia reukaufii SY44-6 showed anti-gout xanthine oxidase inhibitory activity of 49.6% and whitening tyrosinase inhibitory activity of 38.4%, respectively.

• 한국균학회지
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• 제41권1호
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• pp.47-51
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• 2013

국내 자연환경의 효모 균총을 확인함으로써 유용한 효모자원을 확보하기 위한 연구의 일환으로 대전 계족산, 충남 오서산 그리고 전북 백암산 지역에 서식하는 야생화들을 채집하여 이들로부터 효모들을 분리, 동정하였다. 대전의 계족산에서 수집한 야생화로부터는 10종에 속하는 12균주의 효모들을 분리하였고, 충남의 오서산에서 수집한 야생화로부터는 10종의 효모들 17균주를 분리하였다. 또한 전북 백암산의 야생화로부터 모두 24종에 속하는 38균주의 효모들을 분리하였다. 계족산, 오서산, 그리고 백암산에서 모두 발견된 효모는 Cryptococcus 속, Pseudozygoma 속, Rhodotorula 속, Sporobolomyces 속에 속하는 9종의 효모들이었다. 특히 3개 지역에서 수집한 야생화들로부터 Cryptococcus 속에 속하는 Cryptococcus aureus만이 모두 분리되었는데, 이 결과는 각 지역마다 독특한 효모 다양성을 보이고 있음을 시사하는 것이다.