• Archives of Plastic Surgery
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• 제41권3호
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• pp.277-284
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• 2014

Background The most crucial factor in hair transplantation for male pattern baldness (MPB) patients is the efficient utilization of the donor-recipient ratio. However, there is no known factor that scientifically predicts the rate of progression of alopecia or indicates a permanently safe donor area. Methods The study considered 1,008 Korean adult males with MPB; of these, it excluded 56 males with an absence of parietal whorls (PWs). The authors investigated the distance from the vertical bimeatal line (VM) to the PW, from the PW to the upper border of the helical rim (HR), and the distance from the PW to the occipital fringe (OF) in 952 subjects with a PW. Furthermore, we examined the distance from the PW to the OF considering the duration of alopecia and age in 322 subjects with vertex alopecia. Results The distance between the VM and PW varied from 1.5 to 11 cm, with an average distance of 6.25 cm. The PW-HR distance ranged from 3.4 to 17.5 cm, and the average distance was 7.79 cm. The PW-OF distance ranged from 0.5 to 5.5 cm, and the average distance was 2.37 cm. Conclusions For the PW, very large variations existed in the vertical direction. The position of the PW could predict the progression range of the total alopecia of the vertex. Alopecia mostly progresses within 6 cm of the PW toward the occipital side.

• 한국패류학회지
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• 제27권1호
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• pp.77-86
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• 2011

The unwanted artificial oil-spill has severely contaminated the coastal environment in the world. Level of contamination has so far been monitered by various indicator species including mussel, oysters, flounder, and cockle. In this study, we decided to use the oyster as a model organism to observe the morphological changes being exposed to the artificial oil-spill in the coastal areas in Taean, Korea. The oysters were collected from four local sites (Sindu-ri, Uiwang-ri, Jonghyeon-dong, Ansan and Uihang-ri) exposed to various levels of pollution after an oil spill in Taean. Microscopic analysis of the hepatopancreatic microstructure in the digestive gland from the collected oysters show that the swelling, whorl, and destruction phenomenon of the nuclear membrane, a well-known microstructure induced by heavy metal exposure, was observed. Nuclear body (Nb), another typical characteristic of contamination or infection were also observed in some samples. Necrosis was observed in tissue samples collected from the area with a high degree of oil pollution. In addition, parasite-like particles (virus, perkinsus) were observed in most samples. Taken together, these results suggest that oil contamination in the oyster habitats influences the cytopathological changes in Crassostrea gigas.

• 한국지능시스템학회논문지
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• 제13권6호
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• pp.679-685
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• 2003

지문의 중심점과 삼각점은 융선의 흐름이 급격히 변하는 부분에 생기는 부위를 지칭하는 것으로 중심점과 삼각점 주위의 융선의 변화를 관찰하면 중심점과 삼각점의 존재 여부를 추측할 수 있다. 융선의 전역적인 방향성분을 구한 후 같은 방향 성분끼리 군집화를 수행하면 군집화된 부분의 경계에 중심점과 삼각점이 형성되는 것을 관찰할 수 있으며, 궁상문, 솟은 궁상문, 좌제상문, 우제상문, 와상문등 크게 5종류로 분류되는 지문의 문양별 방향성문 군집화의 특성이 다르다는 것을 관찰할 수 있다. 본 논문에서는 삼각점을 획득하지 못한 지문을 이용하여 지문의 중심점을 구한 후 방향성분 군집화를 통하여 지문분류를 효과적으로 할 수 있는 방법을 제안하고 실험을 통하여 이를 증명한다.

• 정보처리학회논문지B
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• 제10B권5호
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• pp.497-502
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• 2003

지문의 분류(Classification)는 대용량 지문 데이터베이스에서 정합시간의 단축과 정확도를 높여주는 역할을 한다. 지문의 종류는 크게 궁상문, 솟은 궁상문, 좌제상문, 우제상문, 와상문의 5종류로 분류되며, 이는 중심점과 삼각점의 개수 및 위치등을 이용하여 분류하고 있다. 기존의 지문 분류는 중심점과 삼각점을 모두 획득하는 회전날인의 경우에 사용 가능한 분류방법이나 현대의 자동화된 실시간 지문인식 시스템에서는 입력센서의 크기 및 입력방법의 문제등으로 인하여 적용할 수 없는 문제점이 있다. 본 논문에서는 이러한 문제점을 해결하기 위하여, 중심점을 획득한 지문을 이용하여 중심점에서 융선의 엔트로피 계측을 기반으로 하며 지문 분류의 정확성을 향상시킬 수 있는 방법을 제안하고 실험을 통하여 이를 증명한다.

• 한국동물학회지
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• 제16권1호
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• pp.43-53
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• 1973

年齡에 따른 미토콘드리아의 構造的變化를 調査하기 위하여 電子顯微鏡을 利用하여 배추흰나비, Pieris rapae L.의 飛筋을 觀察하였다. 미토콘드리아는 크리스타가 잘 발달되지 않은 A型과 크리스타가 복잡하게 발달된 B型의 두種類가 存在하였다. 羽化直後의 것에서는 A型과 B型미토콘드리아가 꼭같이 存在하였고 羽化後 10日 經過한 것에서는 B型미토콘드리아에 比하여 A型이 急速히 減少하였다. 羽化後 10日이 經過된 것의 筋細胞에서는 B型미토콘드리아가 年齡의 增加에 따른 微細構造的變化를 이르켰다. 이러한 老衰된 미토콘드리아의 微細構造的 變化像은 部分的으로 그 內膜이 髓　狀構造를 再形成하는 것으로 나타났는데 이것은 곧 年齡에 따른 退化를 意味한다. 髓　狀構造를 形成하는 것은 많은 同心圓體이며 이 同心圓體의 數와 크기는 年齡과 比例하면서 增加했다. 筋細胞의 細胞質에서 미토콘드리아 內로 侵入한 글리코겐 粒子는 同心圓體의 中央에서도 觀察되었다.

• 대한전기학회논문지:전기물성ㆍ응용부문C
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• 제51권10호
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• pp.500-505
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• 2002

In this paper, fluorescent discharge type ozonizer has been designed and manufactured. Ozone discharge and generation characteristics have been investigated in accordance with a sort of internal electrodes, output voltage of power supply, air flow rate and discharge power. Air has been used as the supplied gas of the ozonizer In this paper, when disuse fluorescent lamp was used as an internal electrode. Air flow rate was varied from 2[1/min] to 10[1/min]. The maximum ozone concentration and yield were 3495[ppm] and 23.4[g/kWh] respectively at 2[1/min] air flow rate and 35.2[W] input power. Illumination was appeared as 5[lx] within 15[cm] from external electrode. Whorl fluorescent discharge type ozonizer was used, air flow rate was also varied from 2[1/min] to 10[1/min]. At this moment, the maximum ozone concentration and yield were 2824[ppm] and 21.7[g/kWh] respectively at 2[1/min] air flow rate and 30.5[W] input power. Illumination was also appeared as 5[lx] within 15[cm] from external electrode like used fluorescent lamp.

• Mycobiology
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• 제48권5호
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• pp.427-430
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• 2020

Ganoderma lingzhi is a well-known source of natural fungal medicines which has been given for the treatment of several diseases. China is one of the major commercial producers of Ganoderma mushroom worldwide. However, with the expansion of the commercial cultivation, the occurrence of the fungal diseases on G. lingzhi has also been increased. The green mold disease symptoms were observed in the cultivation base of G. lingzhi in Zuojia Town, Jilin City, Jilin Province, China, causing the basidiomes to be rotten and withered, and the green mycelium layer generated gradually. The pathogenicity tests showed the same symptoms as appeared naturally in Zuojia mushroom base. Morphology characters revealed conidia green, ellipsoid, globose, 2.56-4.83 × 2.09-4.22 ㎛, length-width ratio was 1.1-1.2 (n = 10). Conidiophores trichoderma-like, often asymmetry, branches solitary, paired or in whorls of 3 phialides formed solitary, paired or in whorl, variable in shape, lageniform, sometimes ampulliform or subulate. While using molecular methodology, comparing with the sequences of Trichoderma hengshanicum from GenBank, the analyzed sequence showed 97.32% homology with the RPB2 sequences, 100% with the TEF1-a sequences. A fungus isolated from the diseased tissues was identified based on morphology and molecular studies as T. hengshanicum. This is the first report of T. hengshanicum causing the green mold disease of G. lingzhi in China.

• 한국가축번식학회지
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• 제16권1호
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• pp.21-38
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• 1992

The present study was carried out to investigate morphologic changes in the corpus luteum of the pregnant rat by electron microscope after administration of prostaglandin F2$\alpha$(PGF2$\alpha$). Pregnant rates were treated with PGF2$\alpha$(1,500$\mu\textrm{g}$/rat) and their corpura lutea were observed morphologically. The results obtained in this study were summarized as follows ; 1. The weight of the ovaries and corpura lutea were decreased slightly at 8~24 hours after PGF2$\alpha$ administratin but no significant differences were observed. 2. The number of corpora lutea and luteal cells decreased slightly at 12~48 hours and 18~24 hours after PGF2$\alpha$ tretment but there were no signifciant differences between control and treatment. 3. The weight of uterus and the unmber of embryo decreased slightly at 96 hours and at 18~96 hours after PGF2$\alpha$ administration but no significant differences were obtained. 4. In the electron microscopic observatons, lipid droplets which are electron dense and appear in the cytoplasm moderately increased in number after PGF2$\alpha$ treatment. The lipid droplets were surrounded by mitochodria and appeared in the autophagic vacuoles. 5. Moderated and high electron dense mitochondria which are round or elongated in shape showed pleomorphism from 3 hours after PGF2$\alpha$ treatment. Destruction of tubular of vesicular cristae was observed at 6 hours after the treatment. Dense body and myelin figures in matrix of mitochondria were also appeared. 6. Well-developed smooth endoplasmic reticulum(sER) showed tubular or vesicular cisternae. A number of whorl membranes containing ribosomes, mitochondria and lipid droplets were observed at 1.5 hour after treatment. sER was abundant in luteal cells at 12 hours were treatment. 7. Well-developed Golgi pparatus appeared obviously 6 hours and more prominently at 12 hours. Those Golgi vesicles were remarkably dilated. 8. Generally, a few rough endoplasmic reticulum (rER) were appeared after treatment and cisternae showed slight dilatation. No differences among the treatments were observed. However, slight dilation of cisternae was observed at 1.5 hours after treatment. 9. Ribosomes composed of free and polyribosomes were abundant before treatment but polyribosomes were appeared at 12 to 24 hours after treatment. 10. Intercellular space were slightly extended at 3 hours and markedly extended at 12 hours. Numerous microvillous protrusions were observed at these times. Membranous multivesicular structures and autophagic vacuoles were also appeared in the intercellular space. 11. At 3 hours after the treatment, autophagic vacuoles appeared in the cytoplasm of the cell. They increased in number with time and were observed to transfer to the intercellular space. Lysosomal dense body appeared in the cytoplasm and the inclusion body was also observed in nucleus at 12 to 24 hours after treatment.

• 한국버섯학회지
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• 제5권1호
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• pp.25-28
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• 2007

본 연구에서는 상황버섯 (P. baumii)의 자실체에 발생하는 Penicillium sp.균을 동정하기 위하여, 분리균의 배양적 형태적 특징을 조사하였다. 분리된 Penicillium sp.의 형태적 특성을 조사한 결과, 분생포자의 모양은 타원형이고 직경이 1.8~2.3 ${\mu}m$ 크기로 분생포자를 많이 형성하였다. 분생포자경은 균사로부터 one-stage branch 모양을 형성하였고, matulae는 3~5개로 길이가 5.2~7.8 ${\mu}m$이며, phialide는 플라스크상이고 크기가 $5.7{\sim}7.5{\times}2.2{\sim}2.7{\mu}m$이였다. Czapek's agar 배지에서 생육시 $25^{\circ}C$에서 8일간 균체가 23 mm 생장하였다. PDA 배지 상에서 균총의 형태는 처음에는 조밀한 솜털모양의 백색을 띄었으며 차츰 기중균사가 형성되었고, 점차 회녹색으로 변하였다. 이상과 같은 형태 및 배양적 특징으로 이 균을 P. rubrum으로 동정하였다. 상황버섯 (P. baumii)에서 P. rubrum에 의한 병은 아직 국내에 보고된바 없어, P. rubrum에 의한 상황버섯 (P. baumii) 푸른곰팡이병을 최초로 보고한다.

• 원예과학기술지
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• 제34권6호
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• pp.924-939
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• 2016

The great economic losses caused by Cucumber mosaic virus (CMV) infection of peppers has led to the development of genetically modified (GM) CMV-resistant peppers. We developed virus-resistant pepper plants using Agrobacterium tumefaciens -mediated transformation. The expressed recombinant protein was purified using nickel-nitrilotriacetic acid resin and immunoaffinity chromatography, and purity was assessed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Immunoblot analysis revealed the purified CMV coat protein (CMV-CP) had a molecular mass of 25 kDa. After in-gel digestion and desalting, the internal peptide fragments of CMV-CP were sequenced by matrix-assisted laser desorption/ionization-time of flight. Most GM pepper and Escherichia coli BL21 internal peptides had identical peptide sequences and contained 137 of 183 whole peptides in CMV-CP. A quantitative enzyme-linked immunosorbent assay was performed to detect CMV-resistant GM peppers. We also provide basic information about the expressed protein in GM peppers for further safety assessment. The contents of soluble protein and CMV-CP were measured in GM and control peppers cultivated in three different areas of Korea. Statistical significance in terms of cultivation areas, harvest times, generations, and plant tissue origin were determined based on a P value of 0.05. The highest amount of CMV-CP was detected at the seedling stage from plant grown in each region. T3 and T5 showed significantly different levels of CMV-CP from T4 in leaves in the whorl stage. No statistical differences were observed among GM peppers at different stages of maturity in any cultivation area. The results from this study contribute to the safety evaluation of newly designed CMV-resistant GM peppers and provide a standard against which to compare other virus-resistant GM peppers.