• Korean Journal of Soil Science and Fertilizer
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• v.45 no.3
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• pp.385-392
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• 2012

This study was conducted to find out the optimum cropping system for the stable production of forage crops in the newly reclaimed land located at Gwanghwal and Gyehwa region of Saemangum reclaimed tide land from October, 2009 to October, 2011. Whole crop barley (WCB), Rye, Italyan-ryegrass (IRG) as winter crops and Corn, Sorghum${\times}$sudangrass hybrid (SSH) as summer crops were cultivated. Soil chemical properties, nutrient uptake, feed value, growth and yield were examinated. The testing soil was showed saline alkali soil where the contents of organic matter, available phosphate and exchangeable calcium were very low, while exchangeable sodium and magnesium were higher. Changes of soil salinity during the growing season of forage crops were less than 0.2%, and the growth of forage crops was not affected by salt injury. Standing rates of winter crops were higher in the order of Rye, WCB, and IRG, while the dry matter yield of winter crops was higher in the order of IRG, Rye and WCB. The highest crude protein (CP) content was recorded in IRG, and total digestive nutrients (TDN) contents were increased in the order of WCB, IRG, and Rye. The TDN content was higher in corn, whereas other feed value was higher in SSH. The content of mineral nutrients on stem, leaf and grain in IRG, Corn were high. After experiment pH was lowed, contents of exchangeable magnesium, sodium and organic matter were decreased while contents of total nitrogen, available phosphate and exchangeable potassium, calcium were increased. Winer crops and summer crops after continually cultivating in cropping system, fresh matter yield increased, compared to WCB-Corn (74,740 kg $ha^{-1}$), IRG-SSH 10%, IRG-Corn 7%, Rye-SSH 6%, Rye-Corn and WCB-SSH 3%. Dry matter yield increased, compared to WCB-Corn (20,280 kg $ha^{-1}$), IRG-SSH 7%, Rye-SSH 6%, IRG-Corn/Rye-Corn/WCB-SSH 3%. The TDN yield increased, compared to WCB-Corn (13,830 kg $ha^{-1}$), IRG-SSH 2%, WCB-SSH and IRG-Corn 1%. Therefore, we suggest that the crop combination of IRG-SSH and WCB-SSH would be preferred for silage stable production.

• Journal of Animal Science and Technology
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• v.51 no.5
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• pp.369-378
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• 2009

Protein fractionation was evaluated from whole crop silages of rye (RS), wheat (WS), triticale (TS), oat (OS), barley (BS), and rice straw silage (RSS), and in vitro trial was carried out to examine the effect of silage and extraction of soluble protein on fermentation characteristics, total gas production and degradation. Soluble protein of silages was extracted with borate-phosphate buffer, and fermentation characteristics, gas production and degradation of silages were estimated by incubating anaerobically the mixed solution of strained rumen fluid and artificial saliva (1:1, v/v) containing dried and ground silages placed in nylon bag at $39^{\circ}C$ up to 48h. Soluble protein (SP) content was lowest for RSS as 2.11% in total CP compared to those for other silages. Highest A fraction (NPN) was observed from RS (74.33% of total CP) while those from TS and RSS were relatively low (48%). B2 fraction was relatively higher for RS, RSS and WS than for TS and BS. $B_3$ fraction was lowest in WS among silages. C fraction (27.07) in RSS was higher than in other silages (1.40~9.93%). pH in incubation solution was increased (P<0.01~P<0.001) for extracted silages up to 12h but decreased (P<0.01) at 48h for non-extracted ones. Contents of ammonia-N (P<0.001) and total VFA (P<0.01~P<0.001) were higher for non-extracted silages than for extracted ones. Acetate proportion was increased (P<0.001) in buffer extracted silages while those of propionate and butyrate were decreased (P<0.001) up to 24h incubation. Increased (P<0.001) total gas production was obtained from non-extracted silages up to 12h while gas production was increased (P<0.01) in extracted ones thereafter. In vitro degradation of dry matter and CP was increased (P<0.001) in non-extracted silages but that of neutral detergent fiber was increased (P<0.001) in extracted ones without difference among silages. Difference in mean values of degradability for each silage prior to- and post extraction with borate buffer, however, was not found among silages. It may be concluded that high NPN content of silages may reduce the protein availability in silages and borate buffer soluble components in silages can stimulate the early stage of fermentation.

• Research in Plant Disease
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• v.8 no.1
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• pp.1-10
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• 2002

Climate in the year of 2001 could be summarized as severe drought from March to May, unusually high temperature in whole plant growth periods and clear weather condition especially in harvesting time of September and October without any typoons. In rice, major diseases such as leaf and panicle blast, bacterial blight, sheath blight and bacterial grain rot developed little due to unfavorable weather conditions, however, brown spot occurred severely due partly to the reduced use of N-fertilizer, Rice stripe virus infection was unusually high mainly in the west coast areas with four times more diseaseD area campared to the previous year, In pepper phytophthora blight was extremely severe in Cheonnam and Cheonbuk provinces, where had frequent rainfalls during growing period. Incidence of major diseases of tomato and cucumber in 2001 was relatively mild. In watermelon, penicillium fruit rot, one of the peculiar disease, spread over major production areas, whereas CGMMV, usually severe in every years was much reduced. Watermelon plants growing in open-fields were more severely diseased than those in plastic houses. Major diseases of chinese melon and strawberry occurred slightly and in particular, sudden wilt syndrome of chinese melon which was severe in 2000 showed mild infections in 2001. Incidence of white rot of garlic and onion was much lower unlike other years due mainly to spring drought and high temperature in the growing period. Infected area of potato bacterial wilt tended to increase in 2001 while fusarium wilt of sweetpotato was decreased. Apple diseases were generally mild, but powdery mildew of pear increased sharply. Barley scab was not severe as seen in other years.

• Journal of The Korean Society of Grassland and Forage Science
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• v.29 no.4
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• pp.337-344
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• 2009

This study was conducted to investigate the effects of various cropping system applied with cattle slurry on productivity of sorghum $\times$ sudangrass hybrid (SSH) and environmental pollution in paddy land. cropping systems used in this study were consisted of two designs, such as double-cropping sorghum $\times$ sudangrass hybrid followed by whole crop barley applied with cattle slurry (DSSCS) and mono-cropping sorghum $\times$ sudangrass hybrid followed by hairy vetch used as green manure (MSSGM). The field experiments were conducted on the clay loam at Backsanmyun, Kimje, Chunlabukdo province in Korea for three years (May 2006 to Apr. 2009). This study was arranged in completely randomized design with three replicates. The yield of dry matter (DM) of SSH in DSSCS increased significantly as compared with that of MSSGM (P<0.05). The contents of crude protein (CP), acid detergent fiber (ADF), neutral detergent fiber (NDF) and total digestible nutrients (TDN) of SSH were not influenced by cattle slurry and green manure. The pH, and contents of OM, T-N and $P_2O_5$ in soil samples collected from DSSCS after the end of experiment were higher than those of MSSGM. The pH, and contents of OM in DSSCS treatment were remarkably higher than those at the beginning of the experiment (p<0.05). However, The pH, and contents of OM in MSSGM treatment were hardly influenced, as compared with those at the beginning of the experiment. The contents of T-N in soil samples collected both from DSSCS snd MSSGM treatments were remarkably higher than those at the beginning of the experiment (p<0.05). In addition, the concentrations of CEC in soil samples collected at the end of the experiment were remarkably higher than those at the beginning of the experiment (p<0.05). The concentrations of $NO_3$-N, $NH_3$-N and $PO_4$-P in leaching water were hardly influenced by the cropping system and application of cattle slurry.

• Magazine of the Korean Society of Agricultural Engineers
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• v.22 no.4
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• pp.82-95
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• 1980

This paper complies the results of the studies so far made on the subsoil improvement of subsurface drainage systems for wet paddy fields (those were located in the Gum-Ho area in Kyung Buk province) which had poor permeability and a high water table. In general, a drainage problem is an excess of water on the ground surface which can effect the productivity and bearing capacity of the soil. With drain pipe systems, (According to their depths and spacing) it may be possible to correct that problem. The experimentation consisted of three test plots, two of which included drain pipe systems with varing depths and width spacing of the pipes. The third plot (C) was an ordinary plot being exempt of a drain pipe system. In detail, the depth of plot A was 80 cm, and the width spacings began at 2. Om and increased by 2. Om up to 10. 0m. The depth of plot B was 60cm and the width spacing was the same as plot A. These tests were performed to research specific details; such as crop yeild, bearing capacity of the soil, the amount of underdrainage, surface cracks, root distribution, the water table level, the consumptive water depth and the soil moisture content. The test period lasted three years, from 1977 thru 1979. The results obtained were as follows: 1. During the test period, the weather conditions for the area tested were in accordance with the annual average for that area. Furthermore the precipitation factor during the spring cultivation season, the intermediate drainage period and the harvest drainage period was of optimum conditions for controling surface cracks, because of less precipitation than evaporation. 2. The difference in the level of the ground water table in plots A and B was hardly noticable, but the difference in the test plots and the ord. plot was greatly noticable. The test plots (A, B) were 30 to 40cm lower than the ordinary plot. On the whole, the ground water table of the ord. plot always stayed at a level of 15-20cm beneath the surface of the soil, the ground water table of the test plot A showed The difference in the depth of the pipe lower than the test plot B, while the test plots showed a remarkable descending effect. 3. The soil temperature in plot A was slightly core than in plot B with a difference of 0. 47$^{\circ}$C, but plot A was 1. 6$^{\circ}$C higher than the ord. plot during the flooding period, but after drainage the temperature difference climed to 2. 0$^{\circ}$C. 4. During the 3rd test year, the values of the cracks were recorded with the values of 59cm in plot A, 42cm in plot B and 15cm in the ordinary plot. Plots A and B had increased 2.5 times the value of the first year while the ordinary plot had remained the same. 5. The root weight of the rice was measured at a value of 77.2 gr. for plot A, 73.5 gr. for plot B and 65.3 gr. for the ord. plot. Therefore, the root growths in plots A and B were much more energetic than in the ord. plot. 6. The consumptive water depth measured during the 3rd year resulted in the values of 26. 0mm per day for plot A, and 24.9 mm per day for plot B, respectively. Therefore, both plot A and plot B maintained the optimum consumptive water depths, but the ordinary plot only obtained the value of 12.3 mm per day, which clearly showed less than the optimum consumptive water depth which is 20 to 30 mm/day. 7. The soil moisture content is in direct relationship to the ground water level. During drainage, test plot A decreased in its ground water level much more rapidly than the other two plots. Therefore, plot A had a much less soil moisture content. But this decreased water level could be directly effected by the weather conditions. 8. The relationship between the bearing capacity and the soil moisture content were directly inversely proportional. It can be assumed that the occurence of soil creaks is limited by the soil moisture content. Therefore, the greater the progress of the surface creaks resulted in a greater bearing capacity. So, tast plot A with a greater amount of surface cracks than the other test plots resulted in a greater bearing capacity. But, the bearing capacity at the harvest season could be effected by the drainage during the intermediate drainage period and by the weather conditions. 9. Comparing the production of the test plots to the ord. plot; there was an increased value of 840kg for plot A, 755kg for plot B and 695kg for the ord. plot in the rough rice. Therefore, plot A had an increase of 20% over the ordinary plot. The possibility of producing double crops was investigated. The effects on barley production in the test plots showed a value of 367kg per 10 acres, which substantiated the possibility of double crops because that value showed an increased value over the average yearly yield for those uplands. 10. So as a result, it can be recommended that by including a drain pipe system with the optimum conditions of an (80cm centimeter) depth and a (l0m) spacing will have a definite positive effect on the over all production capacity and quality of wetpaddy fields.

• The Korean Journal of Pesticide Science
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• v.15 no.4
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• pp.545-572
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• 2011

The genetic diagnosis methods by RT-PCR and Virion capture (VC)/RT-PCR against Rice stripe virus (RSV) were developed. Three diagnosis methods of seedling test, ELISA and RT-PCR were compared in virus detection sensitivity (VDS) for RSV. The VDS of ELISA for RSV viruliferous small brown plant hopper (SBPH) was higher with 40.5% than that of seedling test. The VDS of RT-PCR was higher with 21% than that of ELISA. The VDS of ELISA and VC/RT-PCR was same with 9.2% in average on the SBPH collected from fields at the areas of Gimpo, Pyungtaeg and Sihueng, Gyeonggi province in 2009. The specific primers of RSV for SBPH and rice plant were developed for the diagnosis by Real time PCR. The RQ value of Real time PCR for the viruliferous and non viruliferous SBPH was 1 for 50 heads of non viruliferous SBPH, 96.5 for 50 heads of viruliferous SBPH, 23.1 for 10 heads of viruliferous SBPH + 40 heads of non viruliferous SBPH, and 75.6 for 30 heads of viruliferous SBPH + 20 heads of non viruliferous SBPH. The RQ value was increased positively by the ratio of viruliferous SBPH. Full sequences of 4 genomes of RSV RNA1, RNA2, RNA3 and RNA4 were analysed for the 13 RSV isolates from rice plants collected from different areas. Genetic relationships among the RSV isolates of Korea, Japan and China were classified as China + Korea, and China + Korea + Japan by phylogenetic analysis for RSV RNA1 and RNA2. In case of RNA3 involved in pathogenicity, genetic relationship of RSV among the three countries was grouped into 3 as China, China + Korea, and Korea + Japan. According to the genetic relationships in RSV RNA4, RSV isolates were grouped into 4 as China, Korea, China + Korea + Japan, and Korea + Japan. Viruliferous insect rate (VIR) of RSV in average increased in each year from 2008 to 2010, and the rates were 4.3%, 6.1%, and 7.2%, respectively, at the 28 major rice production areas in 7 provinces including Gyeonggido. The highest VIR in each year was 11.3% of Gyeonggido in 2008, 20.1% of Jellanamdo in 2009 and 14.2% of Chungcheongbukdo in 2010. The highest VIR depending upon the investigated areas was 22.1% at Buan of Jellabukdo in 2008, 36% at Wando and Jindo of Jellanamdo in 2009, and 30.0% at Boeun of Chungcheongbukdo in 2010. Average population density (APD) of overwintered SBPH was 13.1 heads in 2008, 13.9 heads in 2009 and 5.6 heads in 2010. The highest APD was 39.1 and 60.4 heads at Buan of Jellabukdo in 2008 and 2009, respectively, and 14.0 heads at Pyungtaeg of Gyeonggido. The acreage of RSV occurred fields was 869 ha in the western and southern parts, mainly at Jindo and Wando areas, of Jellanamdo in 2008. In 2009, RSV occurred in the acreage of 21,541 ha covered whole country, especially, partial and whole plant death were occurred with infection rate of 55.2% at 3,025 plots in 53 Li, 39 Eup/Myun, 19 Si/Gun of Gyeonggido, Incheonsi, Chungcheongnamdo, Jeollabukdo and Jeollanamdo. Seasonal development of overwintered SBPH was investigated at Buan, Jeollabukdo, and Jindo, Jeollanamdo for 3 years from 2008. Most SBPH developed to the 3rd and 4th instar on the periods of May 20 to June 10, and they developed to the adult stage for the 1st generation on Mid and Late June. In 2009, all SBPH trapped by sky net trap were adult on May 31 to June 1 at Mid-western aeas of Taean, Seosan and Buan, and South-western areas of Sinan and Jindo. The population density of adult SBPH was 963 heads at Taean, 919 at Seocheon and 819 at Sinan area. The origin of these higher population of adult SBPH were verified from the population of non-overwintered SBPH but immigrant SBPH. From Mid May to Mid June in 2010, adult SBPH could not be counted as immigrant insects by sky net trap. The variation of RSV VIR was high with 2.1% to 9.5% for immigrant adult SBPH trapped by sky net trap at Hongsung of Chungcheongbukdo, Buan of Jeollabukdo and so forth in 2009. The highest VIR for the immigrant adult SBPH was 9.5% at Boryung of Chungcheongnamdo, followed by 7.9% at Hongsung of Chungcheongnamdo, 6.5% at Younggwang of Jeollanamdo, and 6.4% at Taean of Cheongcheongnamdo. The infection rate of RSV on rice plants induced by the immigrant adult SBPH cultivated near sky net trap after about 10 days from immigration on June 12 in 2009 was 84.6% at Taean, 65.4% at Buan and 92.9% at Jindo, and 81% in average through genetic diagnosis of RT-PCR. Barley known as a overwintering host plant of RSV had very low infection rate of 0.2% from 530 specimens collected at 10 areas covering whole country including Pyungtaeg of Gyeonggido. Twenty nine plant species were newly recorded as natural hosts of RSV. In winter annual plant species, 11 plants including Vulpia myuros showed RSV infection rate of 24.9%. The plant species in summer annual ecotype were 13 including Digitaria ciliaris with 44.9%, Echinochloa crusgalli var. echinata with 95.2% and Setaria faberi with 65.5% in infection rate of RSV. Five perennial plants including Miscanths sacchariflorus with infection rate of 33.3% were recorded as hosts of RSV. Rice cultivars, 8 susceptible cultivars including Donggin1 and 17 resistant ones including Samgwang, were screened in field conditions at 3 different areas of Buan, Iksan and Ginje in 2009. All the susceptible cultivars were showed typical symptom of mosaic and wilt. In 17 genetic resistant cultivar, 12 cultivars were susceptible, however, 5 cultivars were field-resistant plus genetic resistant to RSV as non symptom expression. When RSV was artificially inoculated at seedling stage to 4 cultivars known as genetic resistant and 3 cultivars known as genetic susceptible, the symptom expression in resistant cultivars was lower as 19.3% in average than that of 53.3% in susceptible ones. In comparison of symptom expression rate and viral infection rate using resistant Nampyung and susceptible Heugnam cultivars by artificial inoculation of RSV at seedling stage, the symptom expression of Heugnam was higher as 28% than 12% of Nampyung. However, virion infection of resistant Nampyung cultivar was higher as 12% reversely than 85% of susceptible Heugnam. Yield loss of rice was investigated by the artificial inoculation of RSV at the seedling stage of resistant cultivars of Nampyung and Onnuri, and susceptible cultivars of Donggin1 and Ungwang for 3 years from 2008. The average yield per plant was 7.8 g, 8.5 g and 13.8 g on rice plants inoculated at seedling stage, tillering stage and maximum tillering stage, respectively. The yield loss rate was increased by earlier infection of RSV with 51% at seedling stage, 46% at tillering stage and 13% at maximum tillering stage. In resistant rice cultivars, there was no statistically significant relation between infection time and yield loss. In natural fields on susceptible rice cultivar of Ungwang at Taean and Jindo areas in 2009, the yield loss rate was increased with same tendency to the infection hill rate having the corelation coefficient of 0.94 when the viral infection was over 23.4%.