• 한국작물학회:학술대회논문집
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• 한국작물학회 2020년도 춘계학술대회
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• pp.120-120
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• 2020

Based on the simple sequence repeat (SSR) marker, a Korean wheat core collection were established with 616 wheat accessions. Among them, the SNP genotyping for the entire genome was performed using DNA chip array to clarify the whole genome SNP profiles. Consequently, a total of 35,143 SNPs were found and we re-established a mini-core collection with 247 accessions. Population diversity and phylogenetic analysis revealed genetic diversity and relationships from the mini core set. In addition, genome-wide association study (GWAS) was performed on 19 phenotypic traits; ear type, awn length, culm length, ear length, awn color, seed coat color, culm color, ear color, loading, leaf length, leaf width, seeding stand, cold damage, weight, auricle, plant type, heading stage, maturation period, upright habit, and degree of flag leaf. The GWAS was performed using the fixed and random model circulating probability unification (FarmCPU), which identified 14 to 258 SNP loci related to 19 phenotypic traits. Our study indicates that this Korean wheat mini-core collection is a set of germplasm useful for basic and applied research with the aim of understanding and exploiting the genetic diversity of Korean wheat varieties.

• Journal of Animal Science and Technology
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• 제66권1호
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• pp.232-236
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• 2024

Ligilactobacillus is a genus of Gram-positive lactobacilli commonly found in the intestinal tracts of vertebrates. It has been granted a Qualified Presumption of Safety (QPS) status from the European Food Safety Authority (EFSA). One specific strain, Ligilactobacillus salivarius B4311, was isolated from fecal samples of broiler chickens from a farm associated with Chung-Ang University (Anseong, Korea). This strain was observed to have inhibitory effects against Listeria monocytogenes. In this paper, we present the complete genome sequence of Lig. salivarius B4311. The whole genome of strain B4311 comprises 2,071,255 bp assembled into 3 contigs representing a chromosome, repA-type megaplasmid, and small plasmid. The genome contains 1,963 protein-coding sequences, 22 rRNA genes, and 78 tRNA genes, with a guanine + cytosine (GC) content of 33.1%. The megaplasmid of strain B4311 was found to contain the bacteriocin gene cluster for salivaricin P, a two-peptide bacteriocin belonging to class IIb.

• Animal Bioscience
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• 제34권7호
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• pp.1105-1115
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• 2021

Objective: To conserve and utilize the genetic resources of a traditional Chinese indigenous pig breed, Liangshan pig, we assessed the genetic diversity, genetic structure, and genetic distance in this study. Methods: We used 50K single nucleotide polymorphism (SNP) chip for SNP detection of 139 individuals in the Liangshan Pig Conservation Farm. Results: The genetically closed conserved population consisted of five overlapping generations, and the total effective content of the population (Ne) was 15. The whole population was divided into five boar families and one non-boar family. Among them, the effective size of each generation subpopulation continuously decreased. However, the proportion of polymorphic markers (P_N) first decreased and then increased. The average genetic distance of these 139 Liangshan pigs was 0.2823±0.0259, and the average genetic distance of the 14 boars was 0.2723±0.0384. Thus, it can be deduced that the genetic distance changed from generation to generation. In the conserved population, 983 runs of homozygosity (ROH) were detected, and the majority of ROH (80%) were within 100 Mb. The inbreeding coefficient calculated based on ROH showed an average value of 0.026 for the whole population. In addition, the inbreeding coefficient of each generation subpopulation initially increased and then decreased. In the pedigree of the whole conserved population, the error rate of paternal information was more than 11.35% while the maternal information was more than 2.13%. Conclusion: This molecular study of the population genetic structure of Liangshan pig showed loss of genetic diversity during the closed cross-generation reproduction process. It is necessary to improve the mating plan or introduce new outside blood to ensure long-term preservation of Liangshan pig.

• Journal of Biosystems Engineering
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• 제16권4호
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• pp.384-398
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• 1991

본 연구는 개별농가(個別農家), 영농단(營農團), 위탁영농회사(委託營農會社)의 농기계 적정투입모형(適正投入模型) 선정(選定)과 보유기종(保有機種)이나 구입희망기종((購入希望機種) 의 경제성을 분석(分析)할 수 있는 대화식(對話式) 시뮬레이션 프로그램을 개발(開發)하고자 수행(遂行)되었으며, 그 결과(結果)를 요약하면 다음과 같다. 가. 농기계(農機械)의 적정투입(適正投入) 규모(規模) 선정(選定) 경제성(經濟性) 분석(分析)의 현실(現實) 적용성(適用性)을 높이기 위하여 투입(投入) 농기계(農機械)의 부담면적(負擔面積) 결정요인(決定要因)과 비용분석을 위한 기초자료를 문헌(文獻) 및 자료조사(資料調査)를 통하여 수집하였으며 이를 기초로 하여 수학적 모델을 개발하였다. 나. 개별(個別) 농업경영체(農業經營體)(농가(農家) 또는 영농단(營農團))의 지역(地域), 기후지대별(氣候地帶別) 적기작업기간에 따른 농기계이용(農機械利用) 일수(日數), 임작업료(賃作業料) 등을, 농촌지도소에 보급된 행정전산용(行政電算用) PC를 이용(利用)하여, 프로그램 이용자(利用者)가 입력설정(入力設定)하고 투입농기계의 경제성(經濟性)을 분석(分析)할 수 있는 대화식(對話式) 프로그램을 개발(開發)하였다. 다. 개발된 프로그램을 이용하여 벼농사 일관작업(一貫作業)은 물론 각 농작업(農作業)을 분리(分離)하여 투입 농기계별로 상세(詳細)한 경제성(經濟性)을 분석(分析)할 수 있으며, 경운 정지작업 기종은 3형식, 이앙(移秧), 방제(防除), 수확작업(收穫作業) 기종은 2형식을 동시(同時)에 분석(分析)할 수 있다. 벼농사는 경운(耕耘), 정지(整地), 방제작업(防除作業)에 대하여 분석할 수 있다. 라. 프로그램 분석결과(分析結果)는 입력(入力)된 경지면적(耕地面積)에서 기계화 영농을 수행할 때 투입 농기계의 기계사용(機械使用) 과부족일수(過不足日數), 부담면적(負擔面積), 손익분기면적(損益分岐面積), 월간(月間) 고정비(固定費), ha당(當) 기계이용비(機械利用費) 등을 포함한다. 마. 손익분기면적(損益分岐面積) 기종별(機種別) 임작업료(賃作業料)를 기준으로 한 손익분기면적(損益分岐面積)은 경운(耕耘) 정지작업(整地作業)의 임작업료(賃作業料)가 100원/평 일때 경운기, 트랙터 22,25,35,38,50,74 마력은 각각(各各) 1.8, 6.7, 7.6, 9.7, 9.3, 17.5, 23.7 ha 였으며, 이앙작업(移秧作業)의 임작업료(賃作業料)가 100원/평일 때 이앙기 보행4조, 승용4,6조는 각각(各各) 2.2, 5.8, 7.9 ha, 수확작업(收穫作業)의 임작업료(賃作業料)가 130원/평 일때 바인더, 콤바인 2,3,4조는 각각(各各) 2.2, 7.2, 10.2, 15.7 ha로 나타났다.

• 한국유기농업학회지
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• 제21권3호
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• pp.391-401
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• 2013

본 연구는 국내 유기축산물(시유) 생산농가에 대한 사양관리 실태 및 생산수준에 관한 조사 연구를 통해 애로사항을 파악하고 개선방안을 모색하고자 수행하였다. 2010년 말 현재 전체 낙농가 6,347호 중 유기축산물(시유) 인증 농가수는 35호로 0.57%였다. 유기축산물 인증농가 35호 중 11농가에 대한 조사결과, 호당 평균 총사육두수는 142두로 그 중 평균 착유우 69두, 건유우 13두, 육성우 및 송아지 60두였다. 성우 대비 후보축의 비율은 42.4%를 차지하고 있었다. 조사농가의 평균 산유량은 분포는 20kg 미만이 14.3%, 25~30kg 미만이 57.1%, 30kg 이상 28.6%로 분포되어 있었다. 조사농가의 유성분 및 유질분포를 보면, 유지율은 3.3%로 전국 평균 유지율 3.95%보다 많이 낮았다. 평균 세균수는 5,775개/ml로 1A 등급, 체세포수는 192,500개/ml로 1등급에 해당되어 유질등급은 우수한 것으로 나타났다. 유기축산물(시유) 생산이 농가소득에 도움이 되는지에 대한 조사결과, 도움이 된다는 응답이 90%로 나타났으나 실제적인 경영자료를 분석한 연구가 추가적으로 수행되어야 할 필요가 있다고 생각된다. 일반 우유 생산에서 유기축산물(시유)로 전환한 이유는 친환경적 목장경영을 위해서라고 응답한 비율이 54.5%로 가장 높았다. 일반 낙농에 비해 유기낙농의 어려운 점으로는 유기사료 공급의 어려움이라고 대답한 비율이 45.5%로 가장 높았다. 실제적으로 유기 조사료 자가 생산 이용률은 43.0%였고, 구입하여 이용하는 비율은 57.0%로 나타나 유기 조사료 자급에 대한 비율을 더 높이는 것이 유기낙농 발전을 위해 해결해야 할 과제임을 알 수 있다. 평균 조농비율은 38.6:61.4로 조사료 급여비율이 매우 낮음을 알 수 있었다. 향후 유기축산물(시유) 확대를 위해서는 유기사료의 안정적인 공급이 우선되어야 할 과제라 생각된다.

• Asian-Australasian Journal of Animal Sciences
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• 제29권3호
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• pp.444-454
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• 2016

This study was designed to examine the characteristics of sawdust and cocopeat bedding materials, including physicochemical properties (Exp. I) and on-farm trial (Exp. II). In Exp. I, the proportion of particle size was in the order of sawdust>cocopeat India>cocopeat Vietnam (p<0.05), and cocopeat contained higher proportion of small particles ($250{\mu}m$+below $250{\mu}m$) than sawdust, causing a dust production problem. Bulk density was cocopeat India>cocopeat Vietnam>sawdust (p<0.05), thus cocopeat treatments showed 4.4 times higher bedding cost than sawdust. The water absorption rates were 702.0% in cocopeat India, 678.3% in cocopeat Vietnam, and 444.0% in sawdust, showing cocopeat had approximately 1.5 times higher water absorption rate than sawdust. Moisture evaporation rates after 12 h of air blowing (2.00 m/s) were higher (p<0.05) in cocopeat Vietnam (80.4%) than sawdust (71.2%) and cocopeat India (72.8%). In vitro ammonia emissions were higher (p<0.05) in sawdust ($2.71mg/m^2/h$) than cocopeat India ($1.59mg/m^2/h$) and Vietnam ($1.22mg/m^2/h$), and total ammonia emissions were higher (p<0.05) in sawdust ($37.02mg/m^2$) than cocopeat India ($22.51mg/m^2$) and Vietnam ($13.60mg/m^2$). In Exp. II, an on-farm trial was conducted with 48 Hanwoo cattle in 16 pens using the same bedding materials as in Exp. I, with fan (blowing 2.00 m/s) and no fan treatments, and feed bunk side (FB) and water supply side (WS) within a pen (4.5 m, $width{\times}9.0m$, length). Beddings were replaced with fresh bedding materials when moisture concentrations were over 65%. No interactions among treatments were detected for moisture concentration and increment rates, and ammonia concentrations, but a significant effect was observed (p<0.01) for each of the treatments. Both concentrations and increment rate of moisture were higher (p<0.01) in the beddings without fan than with fan. Moisture concentrations and increment rate within a pen were also higher (p<0.01) in FB than WS. Thus, the whole no-fan-FB and sawdust-fan-FB were replaced with fresh bedding material between 4 to 5 experimental weeks. The ammonia concentrations and pH of beddings were not significantly different among treatments. Therefore, using cocopeat bedding with a blowing fan can extend twice the bedding utilization period, and WS within a pen showed twice the bedding-life compared to FB. Despite the outstanding characteristics of cocopeat compared with sawdust, using cocopeat as an alternative for sawdust bedding is not recommended for cattle management, considering it has 4.4 times higher bedding cost and a dust production problem.

• 한국근적외분광분석학회:학술대회논문집
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• 한국근적외분광분석학회 2001년도 NIR-2001
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• pp.1621-1621
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• 2001

The quality of sugarcane grown on the NE Australian tropical coast ($16^{\circ}$15'- $18^{\circ}$15' S Lat.) has declined markedly in the past seven years. This has been linked to dilution of mill-supply cane with increasing levels of non mature-stalk material consisting of leaves and sucker culms. The prime research objective was to examine the transition from the pre-harvest, in-field crop to harvested material sent for processing, in terms of quality and crop fraction proportions. A secondary objective was to quantify the effects of preharvest-season crop habit and culm condition on crop quality. Ten quadrat samples from each of 54 random crop sites (17 in 1999 and 37 in 2000), covering a wide range of variables (cultivar, crop class, and edaphic, topographic, climatic, and temporal factors) were collected immediately before harvest. Samples were partitioned into four fractions:- sound and unsound mature stalks (culms), sucker culms, and extraneous matter (leaves). Material harvested from each site was sampled and partitioned into four fractions:- sound and unsound billets (culm pieces), culm-spindle pieces, and leaf. In 2000, before harvest, 14 additional sites were sampled monthly, on three occasions, from March - June. Erect and non-erect culms were divided into sound and unsound classes. All samples were disintegrated and presented to a remote reflectance module of a scanning spectrophotometer using the BSES large cassette module. Near infra-red spectroscopic (NIS) analyses were developed for the rapid determination of quality components (Brix, commercial cane sugar (CCS), fibre, moisture, and polariscope reading). Calibrations for three material groups (culm (n = 639), non-culm (n = 496), and combined) were developed for all components using the 1999 data set. Two sub-sets (n = 178, and 190) of about 10% of the preharvest-season and harvest populations scanned in 2000 also were subjected to full routine laboratory analyses. The 1999 combined calibrations were excellent, but the culm calibrations produced consistently lower standard errors. Non-culm calibrations were marginally better than the combined for only CCS and pol. reading. Analysis of the 2000 culm data with calibrations using all 1999 and 2000 culm data resulted in better predictions relative to the 1999 culm calibrations. This also was true for the combined calibrations. Assessment of quality components in pre- and post-harvest sugarcane using NIS calibrations was more cost effective than using routine laboratory techniques. Outcomes from this NIS-facilitated research will have important economic consequences for the Australian sugarcane industry. Potential CCS present in mature culms is being discounted by dilution with leaves and sucker culms, threatening farm viability. The results question the efficacy of current harvesting technology. The CCS of harvested cane is improved only marginally over that of the in-field crop. Current harvesting technology requires either supplementary, innovative pre-mill processing or a design revolution to improve mill-supply cane quality, and therefore whole of industry economics. NIS-facilitated analyses, before the harvest season, highlighted the benefits of growing erect, sound crops. Loss of CCS then, can be minimized only by a combination of crop improvement and agronomic solutions, applied as part of sound on-farm management.

• 한국식품위생안전성학회지
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• 제31권2호
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• pp.99-106
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• 2016

총호기성균, 대장균군, Escherichia coli, Escherichia coli O157:H7, Listeria monocytogenes, Salmonella spp., Staphylococcus aureus, Bacillus cereus를 대상으로 충청남도 청양의 맥문동 재배 농가 3곳의 미생물 오염도 조사하였다. 그 결과 E. coli O157:H7, L. monocytogenes, Salmonella spp. 는 전혀 검출되지 않았으며, S. aureus는 작업자가 착용한 장갑의 일부에서만 정성적으로 검출되었다. 수확한 맥문동과 접촉가능성이 있는 주변환경(토양, 수확기, 수확용기, 세척기, 세척수, 채반 등)의 미생물 오염도가 전반적으로 낮은 것으로 조사되었는데, 다만 일부 세척기에서 대장균군이 $4.35log\;CFU/100cm^2$ 검출되어 위생관리에 유의할 필요가 있는 것으로 나타났다. 맥문동은 수확 이후에 세척과정과 건조 과정을 거치면서 미생물 오염도(총호기성균, 대장균군, B. cereus)가 감소하는 것으로 나타났다.

• 한국수산과학회지
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• 제42권6호
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• pp.604-608
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• 2009

This study was conducted to evaluate extruded pellet (EP) diet compared with a raw fish moist pellet (MP) diet for flounder, Paralichthys olivaceus on field feeding experiments in Jeju area. The experimental EP diet (juvenile, growing and adult) was prepared based on the former studies on nutrient requirements and feed ingredient of flounder. Fish were distributed randomly to each aquarium as a group of 102,300 fish (initial mean weight 97 g) in field experiment I and reared randomly to each aquarium as a group of 40,000 fish (initial mean weight 120 g) in field experiment II. In field feeding experiment I conducted in commercial flounder farm, survival, wt. gain (94-1,090 g), feed efficiency and condition factor of fish fed experimental EP were comparable to those of fish fed MP, but considerably higher protein efficiency ratio were observed in fish fed experimental EP. In field feeding experiment II, no significant difference was observed in final mean weight of fish fed the experimental EP (1,234 g) and MP (1,286 g), and any noticeable problem caused by feeding the experimental EP was not found during the whole period of feeding trial. Survival of fish fed the experimental EP was lower than that of MP, and there was no significant difference in feed efficiency between the fish fed experimental EP and MP. Therefore, these results strongly suggest that diet EP could be developed to replace MP diet for the marketable size (1.3 kg) of production for flounder without adverse effects on growth performance. In particular, abdominal dropsy and reduction of condition factor, which are frequently occurred in commercial EP feeding farms, were not observed in flounder fed experimental EP.

• 한국컴퓨터정보학회논문지
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• 제20권4호
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• pp.131-138
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• 2015

구매자와의 관계지속을 위한 관계 확산형 비즈니스 모델의 가장 큰 특징은 한번이라도 물품을 구매한 고객은 SNS(Social Networking Service)의 팔로우(Follow) 기능을 이용하여 자동으로 단골고객으로 등록하여, 구매자와 생산자와의 관계가 일회성에 그치지 않고 향후에도 지속될 수 있게 하여 잠재고객이 되고, 장기적으로 재구매가 이루어지게 한다. 단골이 된 고객에게 생산자는 신상품 출하 시 객관적인 물품정보 외에 재배하는 동안의 농장 모습이나 농작물의 성장과정 등 생생한 근황과, 파종에서 수확까지의 숨겨진 이야기를 통해 자신이 농사지으며 전원생활을 하는듯한 감성을 자극한다. 또한, 생산자는 저장법이나 요리법 등의 다양한 사용법을 안내하며 새로운 물품을 추천하거나 홍보를 할 수 있다. 이러한 장점은 기존의 전자상거래에서 상품의 판매와 홍보가 분리되어 링크를 통해 외부로 연결되어야 하는 문제에서 벗어나 판매와 홍보가 하나의 계정 안에서 수행하도록 하여 사이트 접근성을 높여준다. 또한 구매자간에도 상품을 추천하고 소식을 확산하게 하여 구매자는 구매한 상품에 대한 구매경험을 공유하고, 추천, 구매후기 작성 및 기존 구매후기를 재배포하여 서로 알지 못하던 구매자 사이의 소통을 가능하게 한다는 것이다.