• Journal of Sasang Constitutional Medicine
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• v.1 no.1
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• pp.87-112
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• 1989

In order to investigate experimentally the effects of Sung-yangikgibujat'ang (SIT) and Kwangyebujalijungt'ang (KBT) on Yang-insufficient syndrome (陽虛證) induced by Hydrocortisone acetate (H.A.) in experimental animals (Mice and Rat), the author experimented various activities. Delayed type hypersensitivity (DTH), Rosette forming cells (RFC), hemagglutinin (HA) titers, Hemolysin (HL) titers, Body weight, Whole blood viscosity, Plasma Viscosity, Hematocrit, RBC, Albumin, Total protein, Triglyceride, cholesterol and Glucose were measured. The results summerized as follows; 1. In DTH and RFC all the experimental groups were increased significantly in comparison to the H.A.-treated group. 2. In HA titers SIT_treated group were increased significantly and KBT-treated group showed increasing tendancy, but showed no significance. 3. In HL titers all the experimental groups showed increasing tendancy, but showed no significance. 4. In body weight all the experimental groups showed increasing tendancy, but showed no significance. 5. In whole blood viscosity all the experimental groups were decreased significantly in comparison to the H.A.-treated group. 5. In whole blood viscosity all the experimental groups were decreased significantly in comparison to the H.A.-treated group. 6. In plasma viscosity KBT-treated group were decreased significantly and SIT-treated group showed decreasing tendancy, but showed no significance. 7. In Hematocrit SIT-treated group were decreased significantly and KBT-treated group showed decreasing tendancy, but showed no significance. 8. In RBC, albumin and cholesterol all the experimental groups were decreased significantly in comparison to the H.A.-treated group. 9. In total protein and triglyceride KBT-treated group were decreased significantly and SIT-treated group showed decreasing tendancy, but showed no significance. 10. In Glucose SIT-treated group were decreased significantly and KBT-treated group showed decreasing tendancy, but showed no significance. From above findings, it has been demonstrated that Sungyangikgibujat'ang and Kwangyebujalijungt'ang seem to produce the effectiveness on the recovery from depression of the cell-mediated immune response, blood circulation and energy metabolic rate, induced by Hydrocortisone acetate, and in the humoral immune response Sungyangikgibujat'ang have the effectiveness on the recovery, and in cellular component of blood Sungyangikgibujat'ang was more effective than Kwangyebujalijungt' ang, and in plasma of blood Kwangyebujalijungt'ang was more effective than Sungyangikgibujat'ang. Therefore it is suggested that Sungyangikgibujat'ang and Kwangyebujalijungt'ang have the effectiveness on the recovery from Yang-insufficient syndrome more or less.

• Journal of Preventive Medicine and Public Health
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• v.22 no.4 s.28
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• pp.486-494
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• 1989

Lead, iron, and zinc concentrations in whole blood were determined by atomic absorption spectrophotometry, using a simple one-step dilution procedure, which were measured in 3 groups, 98 officers unexposed to dust or to metal, 58 coal miners without pneumoconiosis, and 113 coal workers' pneumoconiosis (CWP) patients. The results were as follows : 1. The precisions (C. V.%) of lead, iron, and zinc in blood were $12.65{\pm}6.95%,\;1.47{\pm}1.25%\;and\;6.35{\pm}3.34%$, respectively. 2. Lead and zinc in blood showed the log-normal distribution unlike iron in blood which showed normal distribution. 3. Lead, iron, and zinc concentrations in blood of 3 groups were follows : There was significant difference of concentration for zinc in blood by groups statistically. 4. The difference of lead, iron, and zinc concentrations in blood was not significant (p>0.05) by profusion on chest radiographs.

• The Korean Journal of Blood Transfusion
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• v.29 no.3
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• pp.240-252
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• 2018

Viscoelastic coagulation tests provide simultaneous measurements of multiple aspects of whole-blood coagulation, including interactions between the plasma components and cellular components of the coagulation cascade. This can be carried out immediately using a point of care technique. Viscoelastic tests could predict the patient's outcome, including mortality, and detect coagulopathy more sensitively, resulted in reduced blood loss. The transfusion strategy based on the viscoelastic parameters rather than a conventional coagulation test has been shown to reduce the transfusion requirements. Although there are concerns about the reliability and accuracy of this method, viscoelastic tests, including ROTEM, would be a useful method to guide patient blood management strategies.

• Journal of Oral Medicine and Pain
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• v.30 no.1
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• pp.15-23
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• 2005

Mucin glycoproteins are the primary carriers of the oligosaccharide moieties that constitute the blood group substances in human saliva. The aim of this study was to determine whether or not the conversion of either the A or B blood group antigens to the H antigen can occur during the degradation process of stored saliva samples. Forty subjects (20 subjects in each A and B blood group) identified as secretors were enrolled in this study. Fresh whole saliva samples and their clarified supernatants were stored at room temperature for 1 week. The conversion of the blood group antigens was detected by SDS-PAGE and immunoblotting. Among the subjects showing the conversion in whole saliva, glandular saliva samples were obtained from 8 subjects (4 subjects in each A and B blood group). Submandibular-sublingual saliva (SMSL) and a mixture of SMSL and parotid saliva (PS) were stored at room temperature for 1 week. The conversion of the blood group antigens was detected by the same method. The obtained results were as follows: 1. In the clarified samples of whole saliva, the A antigen was detected as being either intact (5%) or degraded molecules (95%) after the 1 week period. Conversion of the A antigen to the H antigen was detected in 5 subjects (25%). In the unclarified samples, the A antigen was either detected as degraded molecules (90%) or was not detected (10%). Conversion of the antigen had occurred in 4 subjects (20%). 2. In the clarified samples of whole saliva, the B antigen was detected as intact (20%) or as degraded molecules (65%) or was not detected (15%) after the 1 week period. Conversion of the B antigen to the H antigen was detected in 7 subjects (35%). In the unclarified samples, the B antigen was detected as intact (5%) or as degraded molecules (65%), or was not detected (30%). Conversion of the antigen was observed in 2 subjects (10%). 3. In the glandular saliva samples, only one of the four subjects displayed an antigenic conversion from the A to H antigen or from the B to H antigen. The conversion had occurred in both the SMSL samples and the SMSL and PS mixture. No degradation of the antigens was detected in the other three samples of the A or B blood groups, nor was there any conversion. The results demonstrated that conversion of the blood group antigens could occur in saliva, and suggested that the enzymes responsible for the conversion are present in saliva. Further studies on the origin and activity of the specific glycosidases in saliva as well as quantitative measurements of the antigenic conversion will be needed.

• Journal of the Korean Chemical Society
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• v.45 no.3
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• pp.223-229
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• 2001

A method for the determination of glucose in human whole blood by chemiluminescence method using a stopped flow injection system has been studied. The method is based on the differences in the chemiluminescence intensities of luminol due to the different amounts of hydrogen peroxide produced from the glucose oxidase catalyzed reaction. The enzyme reactor was prepared by immobilization of glucose oxidase on aminopropyl glass beads and the chemiluminescence from a flow cell was measured by means of an optical fiber bundle. In order to obtain the optimum experimental conditions, effects of pH for the chemiluminogenic solution and enzyme reactor, flow rate and temperature on the chemiluminescence intensity were investigated. The calibration curve obtained under optimum experimental conditions was linear over the range from $1.0{\times}10^{-1}$ mM to 7.0 mM and the detection limit was $6.0{\times}10^{-2}$ mM. The proposed method was applied to the determination of glucose in whole human blood sample and the results were compared with those obtained by an official method. The present method was also evaluated by the results of recovery experiments.

• Biomedical Science Letters
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• v.27 no.4
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• pp.216-222
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• 2021

Colorectal cancer (CRC) is major cancer with high incidence and mortality worldwide. It is known that most CRCs arise from precursor adenomatous polyps (APs). Recently, microRNA (miRNA) has been proposed as a biomarker for various cancers including CRC. In this study, the expression patterns of miR-29a in the whole blood (WB) of CRC, AP, and control groups were analyzed by reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) to evaluate the expression level of miR-29a in patients with colorectal neoplasm (CRN) including CRC and AP. As a result, the relative expression of miR-29a was significantly decreased in the patients with CRN compared to the control group (P<0.001). The results were in agreement with previous in vitro cell studies and studies that used tissue and feces samples, suggesting that miR-29a in WB may be useful in demonstrating the status of colorectal tissue. Additionally, we divided the control group into healthy control (HC) without any colorectal symptoms and non-tumor control (NTC) with colorectal symptoms but without any CRN. And then the relative expression of miR-29a was also significantly decreased in the NTC group compared to the HC group (P<0.001). Therefore, our study revealed that miR-29a can differentiate patients with CRN from HC group, but they are also involved in the early stage of inflammatory response and cannot be specific biomarkers for CRN.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.4 no.2
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• pp.167-174
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• 2001

Purpose: Adenovirus infection with swollen Peyer patches in the ileum, known as one of the causes of intussusception, may cause vitamin C depletion in human body because vitamin C is a first line antioxidant. Or low vitamin C status in human body makes the man more susceptible to infection of adenovirus in the ileum with a markedly swollen lymph node. In this study, we tried to find out the relationship between pediatric intussusceptions and vitamin C concentrations of whole blood and plasma. Methods: Whole blood and plasma vitamin C concentrations of fifty-seven patients with intussusceptions from May 1995 to December 1998 at the Gyeongsang National University Hospital were compared with those of 256 normal healthy children. Vitamin C was measured by the 2, 4-dinitrophenylhydrazine methods. Results: The average age of patients (male=39, female=18) with intussusceptions was fifteen months of age. Whole blood vitamin C concentrations of patients and healthy children were $1.49{\pm}0.64$ mg/dL, and $2.18{\pm}0.49$ mg/dL, respectively. Plasma vitamin C concentrations of patients and healthy children were $0.59{\pm}0.36$ mg/dL, $1.47{\pm}0.56$ mg/dL, respectively. But no differences in the vitamin C concentrations of whole blood and plasma according to age, degree of leukocytosis, fever, interval from onset, hematochezia, and need for operation were found. Conclusion: Whole blood and plasma vitamin C concentrations of patients with intussusceptions were lower than those of healthy children (P=0.0001). Prospective studies are needed to elucidate whether these results were consequences or causes of intussusceptions.

• Proceedings of the PSK Conference
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• 2003.04a
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• pp.159.2-160
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• 2003

The aim of this study was to investigate the protective effects of selenium and its combination with ${\beta}$-carotene treatments prior to whole-body irradiation in mice. This was obtained the radioprotective effect of selenium and its combination with ${\beta}$-carotene by evaluation of DNA damage levels in mice spleen and blood after irradiation. (omitted)

• Proceedings of the Korean Society of Veterinary Clinics Conference
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• 2008.05a
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• pp.132-132
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• 2008

• Proceedings of the Korean Society of Life Science Conference
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• 2007.10a
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• pp.89.1-89.1
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• 2007

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