• Title/Summary/Keyword: White rot

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Preliminary Approaches On Decolorization of Blue-Stained Wood Chips By Basidial Fungi

  • Pashenova, N.V.;Hop, Pham Thi Bich;Cho, Nam-Seok
    • Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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    • 2003.11a
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    • pp.9-16
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    • 2003
  • Interaction between wood-destroying basidiomycetes and blue stain fungi were studied during conjoint cultivation on 2% malt extract agar. The ability of basidial cultures to decolourise dark pigments of ophiostomatoid fungi was the main characteristics estimated during this investigation. Antrodiella hoehnelii(S28/91), Bjerkandera fumosa(137), Gleophyllum odoratum(124), Trametes versicolor (B18/91) cultures were found to be the most active in decreasing dark colour of blue stain fungi colonies. The cultures were recommended for further development as agents of biopulping and control of blue stain fungi in wood chips.

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Enhanced Expression of Glucose 2-Oxidase in Phlebia tremellosa by Addition of Phthalates

  • Kim, Baik-Joong;Kim, Hye-Won;Choi, Hyoung-T.
    • Mycobiology
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    • v.39 no.1
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    • pp.64-66
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    • 2011
  • Most fungi possess several hydrogen peroxide-generating enzymes, glucose oxidase and pyranose oxidase. Pyranose oxidase can use glucose as its substrate to generate hydrogen peroxide. White rot fungi, which degrade diverse recalcitrant compounds, contain lignin-degrading enzymes, and lignin peroxidase and manganese peroxidase require hydrogen peroxide for their enzymatic reactions. In this study, we isolated a cDNA fragment of pyranose oxidase from Phlebia tremellosa using PCR and examined its expression under the degradation conditions of diethylphthalate (DEP). Pyranose oxidase expression was enhanced up to 30% by the addition of DEP, and this result supports the possible involvement of pyranose oxidase in the degradation of recalcitrant compounds.

Isolation of a Wood-rotting Fungus to Decolorize a Wide Range of Structurally Different Synthetic Dyes. (다양한 염료의 탈색이 가능한 목재부후균 분리)

    • Microbiology and Biotechnology Letters
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    • v.31 no.3
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    • pp.301-306
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    • 2003
  • Twenty-one different fungi were tested for their ability to decolorize a wide range of structurally different dyes. Twenty fungal strains were isolated from fruiting bodies which were collected at the Kwangneung National Arboretum, Korea. One fungal strain were isolated from a rotting wood at Soongsil University, Korea. Nine kinds of dyes were used: three anthraquinone dyes and six azo dyes. The five fungal strains, Laetiporus sulphureus, Polyporus arcularius. Auricularia polytricha, Stereum ostrea, and Bjerkandera sp. UK-l showed decolorization ability. Except Auricularia polytricha, the four fungal strains were wood rotting fungi, and belonged to Aphyllophorales. Bjerkandera sp. UK-I, which was a white rot fungus, could decolorize all kinds of dyes tested in this study, indicating this fungus is one of candidates for applying in biological methods of dye waste treatment.

Trials for Developing the Measures to Reduce the Inoculum Sources of Apple White Rot (사과 겹무늬썩음병의 전염원 경감법 개발을 위한 시도)

  • 김대희;김이부;엄재열
    • Korean Journal Plant Pathology
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    • v.12 no.2
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    • pp.219-255
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    • 1996
  • 사과 겹무늬썩음병의 주 전염원은 가지 병반에서 형성되는 자낭포자 및 병포자이므로, 가지에서의 포자형성 또는 포자의 분산을 화학적 또는 물리적 방법으로 저해할 수만 있다면 효과적으로 병을 방제할 수 있을 것으로 생각했다. 전염원을 줄이기 위한 첫 번째 시도로 1992년 이른봄 가지에 형성된 사마귀를 호미로 제거한 결과, 병포자의 분산과 과실발병 억제효과에 있어서 무처리와 유의적인 차이가 인정되지 않았다. 두 번째 시도로 포자의 분산을 막기 위해 1993년 이른봄에 겹무늬썩음병에 걸린 가지를 비닐필름으로 감고 5월부터 9월까지 20일 간격으로 살균제를 살포한 결과, 통계적으로 유의성이 인정되는 병 방제효과가 있었고, 나무의 생육에 있어서도 콜크층이 이상 비대하는 것 이외에 별다른 이상이 없었다. 이 개념을 더욱 발전시켜 1994년 이른봄에는 병든 가지를 수용성 고분자 물질인 polyvinyl alcohol로 도포하고 수배 분산 포자의 수를 조사한 결과, 포자의 분산량이 크게 줄어져 실용화의 가능성이 시사되었다.

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Submerged Culture of Phanerochaete chrysosporium and Lignin Peroxidase Production (Phanerochaete chrysosporium의 액체 배양 및 Lignin Peroxidase 생산)

  • Park, Se-Keun;Jeong, Myoung-Sun;Kim, Yeong-Kwan
    • Journal of Industrial Technology
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    • v.21 no.A
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    • pp.343-349
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    • 2001
  • This study characterizes the growth of white rot fungi Phanerochaete chrysosporium IFO 31249) and lignin peroxidase(LiP) activity in different submerged culture media. P. chrysosporium was grown in the form of pellet of various sizes from a spore inoculum under shaking liquid culture condition. While the growth of mycelia was higher under the nitrogen-sufficient culture than under the nitrogen-limited culture, ligninase activity was relatively lower. The lignin peroxidase appeared in nitrogen-limited culture and was suppressed by excess nitrogen. High level(40U/l) of lignin peroxidase activity was obtained in the growth medium containing 1.5mM veratryl alcohol, a secondary metabolite of P. chrysosporium. Lignin peroxidase production was not observed under conditions of nitrogen sufficiency or in balanced media, suggesting that control parameters could increase the activity by manipulating the secondary metabolism.

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Cytochalasin E Production by Rosellinia necatrix and Its Pathogenicity on Apple (사과흰날개무늬병균의 Cytochalasin E 독소 생산과 병원성)

  • Lee, Dong-Hyuk;Choi, Kyung-Hee;Uhm, Jae-Youl
    • Research in Plant Disease
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    • v.15 no.1
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    • pp.46-50
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    • 2009
  • Cytochalasin E (CE) is a secondary metabolite secreted by Rosellinia necatrix, caused by white root rot, and has toxicity to apple as a toxin during disease progress. This study was conducted to demonstrate the relationship between the production of CE and its pathogenicity. CE producing isolates and non-producing isolates of R. nectatrix were isolated from the mycerial mat of diseased roots and was detected on that using a TLC and HPLC analysis and in vivo pathogenicity test. CE non-producing isolates were not pathogenic to apple roots and not detected CE by TLC and HPLC analysis. It was shown that the production of CE was related to the pathogenicity of R. nectatrix.

Studies on the Ligninolytic Enzyme Activities During Biological Bleaching of Kraft Pulp with Newly Isolated Lignin-Degrading Fungi

  • Lee, Seon-Ho
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.31 no.2
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    • pp.8-14
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    • 1999
  • A screening has been performed to find hyper-ligninolytic fungi, which degtrade beech and pine lignin extensively in order to broaden the understanding of the ligninolytic enzymes elaborated by various white-rot fungi. One hundred and twenty two ligninolytic strains were selected from decayed woods with a selective medium for screening ligninolytic wood-rotting fungi. Two of them, Phanerochaete sordida YK-624 and YK-472, showed much higher ligninolytic activity and selectivity in beech-wood degradation than typical lignin-degrading fungi, phanerochaete chrysosporium and Coriolus versicolor. They also degraded birch dioxane lignin and residual lignin in unbleached kraft pulp(UKP) much more extensively than P. chrysosporium and C. versicolor. During fungal treatment of beech wood-powder, the fungus strain P. sordida YK-624 showed higher activity of extracellular manganese peroxidase (MnP) in the medium than P. chrysosporium. It also showed MnP activity, which would not be lignin peroxidast during treatment of oxygen-bleached kraft pulp(OKP) and under enzyme-inducing conditin.

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Studies on the Treatment of Pulp Bleaching Effluent with KS-62 Fungus (KS-62 균주에 의한 펄프 표백 폐액처리에 관한 연구)

  • 조준형;은주영
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.32 no.1
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    • pp.86-93
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    • 2000
  • High Colored kraft bleaching effluent is one of the main constrains in pulp and paper industry due to its dissloved lignin derivatives. The degradation of lignin in pulp and paper mill effluent is mainly caused by white-rot fungi. This paper showed that the treatment with KS-62 fungus significantly reduced the color and chemical oxygen demand in the effluent. The amounts of Mn ions in the wastewater would play roles in the induction and activity of MnP (Managanese peroxidase). Extracellular MnP was isolated from the fungus KS-62. The treatment with the MnP had the most effective decolorizatiion in the wastewater treatment using nutrients mediu.

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