• 한국미생물·생명공학회지
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• 제29권2호
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• pp.110-114
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• 2001

가축 사료첨가용 probiotics용 균주를 얻기 위해 특히 내열성이 높아 가공사료에 적합한 Bacillus sp.를 위주로 하여 가축에게 유용한 효소인 phytase를 비롯하여 protease, cellulase, xylanase, amylase의 활성을 모두 나타내는 4-3 균주를 얻어 동정한 결과 Bacillus subtilis로 밝혀졌으며, 이를 B. subtilis 4-3으로 명명하였다. 본 균주를 원료사료에 순수배양하여 사료의 항영양인자인 phytic acid 분해율을 검토한 결과 대두박 및 쌀겨에 있어서는 phytic acid 분해율이 낮았으나, 밀기울의 경우 80.63%로 상대적으로 높은 phytic acid 분해율을 나타내었다. 원료 사료를 이용한 균주의 생산 조건은 대두박 1%(w/v)와 당밀 2%(w/v)가 가장 적합한 균주 생산을 위한 배지조성으로 검토되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제23권9호
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• pp.1198-1204
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• 2010

This study was conducted to investigate the effects of dietary supplementation with multiple enzymes composed of phytase plus carbohydrolases (ROVABIO$^{(R)}$ Max, RM) on growth performance, nutritional availability and intestinal viscosity in broiler chicks. A total of one thousand, one-day-old male broiler chicks were randomly allotted into treatment groups that received one of five experimental diets for 32 days. Each group consisted of 40 birds and all experiments included five replicates. The dietary treatments included PC (a positive control diet), NC1 (65 kcal/kg, 0.15% and 0.10% less ME, available phosphorus and calcium levels, respectively, than the PC diet), NC2 (85 kcal/kg, 0.20% and 0.10% less ME, available phosphorus and calcium levels, respectively, than the PC diet), NC1+RM (NC1 plus ROVABIO$^{(R)}$ Max) and NC2+RM (NC2 plus ROVABIO$^{(R)}$ Max). The average body weights, daily body weight gains and feed conversion rates of the chicks fed a diet containing RM improved significantly or tended to improve. The treatments also had no effect on the carcass characteristics or blood parameters, but the viscosity of the intestinal contents of the chicks fed the diet containing RM was significantly lower than that of chicks in the NC without RM groups. Additionally, chicks fed the dietary RM showed increased breaking strength and ash content of the tibia when compared to chicks that received the non-RM diets. Taken together, the results of the present study indicated that the addition of multiple enzymes consisting of phytase plus NSP enzymes improved the growth performance and mineral status of the tibia in broiler chickens fed corn-wheat-soybean meal-based diets with reduced levels of nutrients. Further, these findings suggest that the improved animal performance is associated with reduced intestinal viscosity by the dietary enzyme complex.

• Asian-Australasian Journal of Animal Sciences
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• 제14권10호
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• pp.1440-1443
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• 2001

Forty eight $Duroc{\times}Yorkshire{\times}Landrace$ pigs ($15.2{\pm}0.3kg$ average initial BW) were used conducted to evaluate the effects dietary phytase (Phytezyme, WOOJIN, Co. Ltd) supplementation on the growth performance and nutrient digestibility in growing pigs. There were three pigs per pen and four pens per treatment. Treatments were 1) Con (corn-wheat-SBM), 2) PE0.1 (low-P iet+0.1% Phytezyme), 3) PE0.2 (Iow-P diet+0.2% Phytezyme), 4) PE0.3 (low-P diet+0.3% Phytezyme). During d 0 to 28, average daily gain was not significantly different among the treatments. Pigs fed PE0.3 diet significantly decreased (p<0.05) their average daily feed intake compared to pigs fed Con diet. Also, gain/feed in pigs fed PE0.1 and PE0.3 diet was improved (p<0.05) compared to pigs fed Con and PE0.2 diet (p<0.05). For d 28 to 56, pigs fed PE0.2 diet grew significantly faster (p<0.05) than pigs fed Con and PE0.1 diet. Gain/feed was greater (p<0.05) for PE0.2 and PE0.3 treatments than for Con. For overall period, average daily gain was increased (P<0.05) by the addition of 0.2% Phytezyme compared with Con. Gain/feed was significantly improved (p<0.05) by supplementation of PE0.3. Pigs fed PE0.2 showed increased DM and N digestibilities compared to pigs fed other treatments. Supplemented diets PE0.2 and PE0.3 improved (p<0.05) the apparent digestibility of Ca and P compared to other treatments. In conclusion, the results obtained from these feeding trials suggest that the Phytezyme supplementation of diets for growing-finishing pigs had improved growth performance and nutrient availability.