• 한국식품영양과학회지
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• 제14권2호
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• pp.188-191
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• 1985

퇴비 숙성초기에 고온성 cellulose 분해이용균으로 분리된 Herpetosiphon geysericola CUM 317균주는 전분분해 효소인 ${\alpha}-amylase,\;{\beta}-amylase$ 및 glucoamylase를 모두 생성한다. 이 균을 사용하여 그 배양조건을 달리하여 각 amylase의 생성관계를 서로 비교한바 50℃의 밀기울 고체배지나 $40^{\circ}C$의 액체배지상에서 ${\beta}-amylase$는 배양초기 10시간만에 최대의 생성력가를 보였는 반면, ${\alpha}-amylase$와 glucoamylase는 30 내지 40시간 정도의 배양말기에 최대를 이루었다. Polypeptone을 함유한 액체배지에 탄소원의 첨가나 무기질소원의 첨가는 전반적으로 amylase들의 생성이 크게 저하되었으나 cellulose에 의해서 glucoamylase의 경우 150% 정도 증가되었다. 액체배지에 $CuSO_4$를 첨가해 줌으로서 ${\alpha}-amylase$만의 생성증가 효과를 얻었고 $CdSO_4$에 의하여 ${\beta}-amylase$만의 생성증가가 있었으며, 그리고 $CaCl_2$에 의하여 glucoamylase만의 증가효과가 있은 반면, 상대적으로 ${\beta}-amylase$의 급격한 감소가 일어났다. 이들 amylase들의 최적 효소생성 pH는 7.5였으며, 최적온도는 ${\alpha}-amylase$와 glucoamylase의 경우 $40^{\circ}C$였고 ${\beta}-amylase$는 $30^{\circ}C$였다.

• BMB Reports
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• 제37권4호
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• pp.422-428
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• 2004

Raw-starch-digesting $\alpha$-amylase (Amyl III) was purified to an electrophoretically pure state from the extract of a koji culture of Aspergillus awamori KT-11 using wheat bran in the medium. The purified Amyl III digested not only soluble starch but also raw corn starch. The major products from the raw starch using Amyl III were maltotriose and maltose, although a small amount of glucose was produced. Amyl III acted on all raw starch granules that it has been tested on. However, it was considered that the action mode of the Amyl III on starch granules was different from that of glucoamylase judging from the observation of granules under a scanning electron microscope before and after enzyme reaction, and also from the reaction products. Glucoamylase (GA I) was also isolated and it was purified to an electrophoretically pure state from the extract. It was found that the electron micrographic features of the granules after treatment with the enzymes were quite different. A synergistic effect of Amyl III and GA I was observed for the digestion of raw starch granules.

• Mycobiology
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• 제43권4호
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• pp.467-474
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• 2015

Development of efficient substrate formulas to improve yield and shorten production time is one of the prerequisites for commercial cultivation of edible mushrooms. In this study, fifteen substrate formulas consisting of varying ratios of palm press fibre (PPF), mahogany sawdust (MS), Gmelina sawdust, wheat bran (WB), and fixed proportions of 1% calcium carbonate ($CaCO_3$) and 1% sucrose were assessed for efficient Lentinus squarrosulus production. Proximate compositions of mushrooms produced on the different substrate formulas were also analysed and compared. Substrate formulations containing 85% PPF, 13% WB, 1% $CaCO_3$, and 1% sucrose were found to produce the highest carpophore yield, biological efficiency and size (206.5 g/kg, 61.96%, and 7.26 g, respectively). Days to production (first harvest) tended to increase with an increase in the amount of WB in the substrate formulas, except for PPF based formulas. The addition of WB in amounts equivalent to 8~18% in substrate formulas containing 80~90% PPF resulted in a decrease in the time to first harvest by an average of 17.7 days compared to 80~90% MS with similar treatment. Nutritional content of mushrooms was affected by the different substrate formulas. Protein content was high for mushrooms produced on formulas containing PPF as the basal substrate. Thus, formulas comprising PPF, WB, $CaCO_3$, and sucrose at 85% : 13% : 1% : 1%) respectively could be explored as starter basal ingredients for efficient large scale production of L. squarrosulus.

• Journal of Ginseng Research
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• 제3권1호
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• pp.54-65
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• 1979

To investigate the feeding value of the concentrated feedstuff including the Korean ginseng meal instead of wheat bran, Holstein milk cows were fed by this feedstuff (10kg/day) for 80 days, and the amounts of milk production and quality of milk were checked. The results are as follows: 1. Milk production of cows fed by the concentrated feedstuff including the ginseng meal was increased as averaged as 1.25kg/day compared with those of controlled cows during same period(SA) and before feeding treatment (SB). Significant different of milk production was found between cows treated and cows of SB plot, but there was no significant difference of milk production between cows treated and cows of SA plot. 2. Milk fat content was significantly increased as moth as 0.22%, 0.69% and 0.455% by the feeding of ginseng meal compared with that of SB Plat and that of SA Plot, and as average value, respectively. 3. Solid not fat (SNF) of milk from cows fed by the ginseng meal was also significantly increased as much as 0.845%, 1.339% and 1.0925 tempered with these of cows before treatment (SB) and cows controlled (SA), and as average value, respectively. 4. Specific gravity of milk produced from cows treated was higher as average as 0.0055 than those of controlled plots (SA and SB). Significant difference of specific gravity was found between cows treated and cows of SA plot, but there was no significant difference between tows treated and cods of SB plot. 5. Acidity of milk produced from cows treated was lower as averaged as 0.0045 than those of controlled plots (SA and SB). Significant difference between cows treated and cows of SA plot was found, but there was no significant difference between cows treated and cows treated and cows of SB plot.

• 미생물학회지
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• 제5권2호
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• pp.43-54
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• 1967

The characteristics of enzymic sources and its effective uses concerned with brewing of Yack-Tack Joo which is traditional and popular liquors for all Korean have been studied. Results obtained are as follows; 1)Kock Ja (enrich of fungi and yeast produced in Korean brewery) is found to be weak in its liquifying(600U.) and saccharifying activity(1300U.), so that it is useful to conbine two factors effectively for better brewing. 2) The additional ratio of Kok Ja per materials is seems proper at line of 20 percent for better fermentation and the enzymic preparations inoculated of microorganisms in wheat bran is seems proper at 25 percent line. 3) Adding the enzymic preperation in which the strain Rhyzopus had been inoculated to the experimental mash at 5 percent per material, the rate of fermentation was revealed highest degree than those of else. 4) It is not proper to add a single Bun Kok in fermentation, as it produce much acid in mash during brewing. 5) However, the enzymic preparation composed of Asp usami and Rhyzopus sp. produced less acid in brewing. 6) The increasing of temparature in enzymic samples, temparatures of the mixtured Kuk(Kok Ja and enzymic preparation) are higher than those of single addition at the first stage in pre-fermentation, but there are no differences at the late stage of post-fermentation. 7) Amount of amino acids in the plot of enzymic prepation are found much more than those of single use at late stage of post-fermentation. In the plot of single use of Kock Ja, the amount was the most than else, the proteinase activity is strongest more than else. 8) In the brewing of Korean Tack-Yack-Joo, it is desirable less amount of acidity, more amount of amino acid, stronger liquifaction of starch and vigorous saccharification. Thren it was found that the application of two prepations(Kock Ja and Bun kok) is most effective to get moderate quality in Tack-Yack-Joo brewing.

• 한국식품영양과학회지
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• 제21권2호
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• pp.187-194
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• 1992

Rhizopus oryzae CJ-2114의 polygalacturonase 생성을 위한 최적조건은 수분이 60% 함유된 밀기울 배지에 1% albumin, 0.2% (NH$_4$)$_2$C$_2$O$_4$, 1% sorbitol을 첨가하여 96시간 배양시 최대활성을 나타내었으며, Sep-hadex G-75 및 G-150을 사용한 gel filtration과 DEAE-cellulose에 의한 이온교환 크로마토그라피를 통하여 이 효소를 11.13배 정제할 수 있었고, 수율은 40.3% 였다. 정제효소는 polyacrylamide gel 전기 영동에 의하여 단일밴드로 확인되었으며 분자량은 SDS-polyacryl-amide 전기영동에 의하여 47,000정도로 측정되었다. 효소의 결정구조는 표면이 거친 기둥모양을 형성하고 있었으며 아미노산 조상은 17종류로써 glutamic acid 함량이 198.74mg/g enzyme로 가장 많았다.

• 한국수정란이식학회지
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• 제28권4호
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• pp.325-335
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• 2013

The study was set for one year to measure the effects of concentrate supplementation on reproductive performances and semen quality in indigenous rams. The study was conducted at the Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh during the period from May 2011 to April 2012. Forteen ram lambs (4~5 months) were randomly divided into two equal groups (n=7); supplemented vs control. The animals of control group were maintained on natural grazing. Along with natural grazing the supplemented group was on supplemented feeding. The concentrate supplementation (Wheat bran, Crushed maize, Soy bean meal, Fish meal, DCP powder, Vitamin mineral premix, Salt) was provided @ 300 g/head /day to the supplemented group. Body weight, scrotal circumference, BCS and libido index were measured weekly. Age, body weight and scrotal circumference at puberty were recorded. Semen was collected once in a weak using artificial vagina and chilled at $5^{\circ}C$ for 48h for evaluation. Concentrate supplementation did not influence (p>0.05) body condition score, age, weight, scrotal circumference at puberty and libido index. Final body weight (kg), growth rate (g/d), scrotal circumference (cm) and scrotal growth rate (mm/15d) were significantly (p<0.05) higher in supplemented group of rams compared to control. Volume, concentration, motility and membrane potentiality of spermatozoa were varied significantly (p<0.05) in supplemented and control groups. However, density, mass motility, viability and sperm with normal acrosome, midpiece and tail were not differed insignificantly (p>0.05) in different observation times. It was concluded that concentrate supplementation with free grazing improved weight and scrotal circumference gain and semen production with increased quality in indigenous ram.

• Animal Bioscience
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• 제34권4호
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• pp.633-641
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• 2021

Objective: The effects of adding fermented feed to a pelleted total mixed ration (PTMR) on the growth performance of lambs remain unclear. The present study aimed to investigate the feed efficiency and productivity of lambs that were fed PTMR containing fermented soybean meal (FSM) or wheat bran (FWB). Methods: Sixty 90-d-old hybrid lambs were randomly allocated into 12 pens (5 lambs/pen) that were randomly assigned to 4 dietary treatments (3 pens/treatment) with PTMR (basal diet), 2% FSM, or Lactobacillus- or yeast-FWB (L-FWB or Y-FWB) addition in the basal diet. Results: The findings showed that lambs fed 2% FSM supplemented diet had enhanced (p<0.05) average daily gain (ADG) and carcass yield (p = 0.015), while they had a decreased (p = 0.006) feed conversion ratio compared to that of other three groups. Inclusion of FSM or FWB in PTMR improved (p<0.05) the nutrient digestibility, while it reduced the urea nitrogen content in serum compared to the PTMR group. Additionally, the decreased ratio of N excretion to ADG (p<0.01) was observed with FSM and L-FWB supplementation compared with the PTMR and Y-FWB groups. Conclusion: In conclusion, feeding the fermented feed-supplemented diet improved nutrient digestibility and growth performance, and 2% FSM-supplemented diet exhibited superior production-promoting efficiency to lambs.

• Journal of Microbiology and Biotechnology
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• 제32권4호
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• pp.437-446
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• 2022

In this study, to obtain icaritin with high pharmacological activities from icariin, which has a content ratio of over 58% in the total flavonoids of Epimedium herb, a special Epimedium flavonoid-glycosidase was produced, purified and characterized from Aspergillus sp.y848 strain. The optimal enzyme production was gained in a medium containing 5% (w/v) wheat bran extract and 0.7% (w/v) Epimedium leaf powder as the enzyme inducer, and strain culture at 30℃ for 6-7 days. The molecular weight of the enzyme was approximately 73.2 kDa; the optimal pH and temperature were 5.0 and 40℃. The enzyme Km and Vmax values for icariin were 15.63 mM and 55.56 mM/h. Moreover, the enzyme hydrolyzed the 7-O-glucosides of icariin into icariside II, and finally hydrolyzed 3-Orhamnoside of icariside II into icaritin. The enzyme also hydrolyzed 7-O-glucosides of epimedin B to sagittatoside B, and then further hydrolyzed terminal 3-O-xyloside of sagittatoside B to icarisiede II, before finally hydrolyzing 3-O-rhamnoside of icarisiede II into icaritin. The enzyme only hydrolyzed 7-O-glucoside of epimedin A or epimedin C into sagittatoside A or sagittatoside C. It is possible to prepare icaritin from the high-content icariin in Epimedium herb using this enzyme. When 2.5% icariin was reacted at 40℃ for 18-20 h by the low-cost crude enzyme, 5.04 g icaritin with 98% purity was obtained from 10 g icariin. Also, the icaritin molar yield was 92.5%. Our results showed icaritin was successfully produced via cost-effective and relatively simple methods from icariin by crude enzyme. Our results should be very useful for the development of medicines from Epimedium herb.

• 한국포장학회지
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• 제28권1호
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• pp.23-30
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• 2022

본 연구에서는 바이오매스 부산물을 바이오 플라스틱에 첨가할 수 있는지 여부를 찾고자 바이오매스 부산물인 왕겨, 옥 피, 밀박, 대두박에 대해 일반성분을 조사하였다. 그리고 왕겨에 대해 분체화, 표면 개질 특성을 조사하였다. 부산물 중에서는 왕겨의 입도가 6.44 ㎛로 가장 적합하였으며, silane 처리 후 ESO(Epoxidiz soybean oil)와 3-aminopropyl triethoxysilane를 1:1로 혼합한 용액을 2% 첨가 시 표면개질 상태가 가장 우수하였고, SEM에 의한 입자형상도 가장 좋은 것으로 나타났다. 따라서 ACM(air classifying mill) 처리한 왕겨를 silane 처리한 후 혼합용액을 2% 첨가하는 것이 표면이 개질된 바이오플라스틱 원료로 첨가하여 사용할 수 있을 것으로 사료되었다.