• Title/Summary/Keyword: Western Region

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Identification of a Regulatory Region within the luxR Structural Gene in a Marine Symbiotic Bacterium, Vibrio fischeri

  • Choi, Sang-Ho
    • Journal of Microbiology and Biotechnology
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    • v.4 no.3
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    • pp.176-182
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    • 1994
  • The light-organ symbiont of pine cone fish, Vibrio fischeri, senses its presence in the host and responds to environmental changes by differentially expressing its symbiosis-related luminescence genes. The V. fischeri luminescence genes are activated by LuxR protein in the presence of an autoinducer. In an effort to elucidate the mechanism of regulation of luxR, a plasmid containing luxR was mutagenized in vitro with hydroxylamine and a luxR mutant plasmid was isolated by its ability to activate luminescence genes cloned in E. coli in the absence of the autoinducer. The specific base change identified by DNA sequencing was only single base transition at +78 from the transcriptional start of luxR. Based on a Western immunoblot analysis, the nucleotide change directed the synthesis of much higher level of LuxR protein without any amino acid substitutions. The results suggest that the region including the +78th base is presumably internal operator required for autorepression of luxR, and the increased cellular level of LuxR results in activation of luminescence genes by autoinducer independent fashion.

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The Treatment of Gunshot Wound with Maxillofacial Fracture in a Dog

  • Kim, Young-ung;Lee, Dong-bin;Heo, Su-young;Kim, Nam-soo
    • Journal of Veterinary Clinics
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    • v.35 no.5
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    • pp.215-217
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    • 2018
  • A one-year-old, intact male, 24 kg, mixed breed dog was referred to the Animal Medical Center, Iksan, Chonbuk, Korea for treatment of a gunshot wound to the head. Physical examinations revealed bilateral nasal bleeding and open-mouth breathing. Radiographic examination showed fracture of the right maxilla bone and multiple fractures of the nasal bone. A $1cm{\times}1cm{\times}1.8cm$ region of mineral opacity material was observed in the right-cranial ventralnasal cavity and a $6mm{\times}6mm{\times}9mm$ region of mineral opacity material was present in the left-cranial dorsal-nasal cavity. The surgical procedure involved removal of bone fragments and the lodged bullet as well as the installation of three intraosseous wires. At two weeks after surgery, the patient exhibited no complications and had a good prognosis.

Sero-epidemiology of transmissible gastroenteritis of pigs in Gyeongbuk province (경북지방 돼지의 전염성 위장염에 대한 혈청학적 역학조사)

  • 조광현;박최규;김영환;박인화;김성국;박노찬;정종식
    • Korean Journal of Veterinary Service
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    • v.24 no.3
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    • pp.271-278
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    • 2001
  • Swine sera collected from January to December 2000 were tested for the survey on transmissible gastroenteritis(TGE) infection in Gyeongbuk province. Serum neutralization(SN) test was peformed in finishing pigs of 50 pigs industry without clinical signs of TGE and sows, gilts and growing pigs of four pigs industry with TGE. As the results of SN test in fifty industry, antibody titers of 90.6%(474/523 samples) showed below 4. Antibody titers of them showed highly in two pigs industry of western region and three pigs industry of southern region of Gyeongbuk province. The results indicated that TGE had been occurred before in five pigs industry. Most of antibody titers showed of highly in four pigs industry having had TGE. There were no significant differences of the antibody titers of TGE according to age when the survey was made. The above results indicated that the pigs of Gyeongbuk province were not almost exposed in porcine respiratory coronavirus(PRCV) by present.

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Epidemiology of Esophageal Cancer in Kazakhstan

  • Igissinov, Saginbek;Igissinov, Nurbek;Moore, Malcolm A.;Kalieva, Zhansaya;Kozhakhmetov, Saken
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.3
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    • pp.833-836
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    • 2012
  • The incidence of esophageal cancer in Kazakhstan was analysed for the period of 1989-2010 years, with a focus on trends by age, gender and region. The highest rates of incidence were evident in the Western regions. The dynamics for cancer of the esophagus in the Republic demonstrated decrease, except in the Karaganda region, where a tendency for increase was noted. Considerable variation between regions pointed to differences in risk factor exposure which need to be targeted for future control efforts.

Anti-correlated hard and soft X-ray lags in the Z source GX 5-1

  • Sriram, K.;Choi, C.S.;Rao, A.R.
    • The Bulletin of The Korean Astronomical Society
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    • v.36 no.2
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    • pp.149.2-149.2
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    • 2011
  • We performed the cross-correlation analysis on energy-dependent light curves of the Z-type source GX 5-1. We observed X-ray delays of a few hundred seconds between hard (16-30 keV) and soft (2-5 keV) X-ray light curves. During these phenomena, the centroid frequency of horizontal branch oscillation (HBO) was found to shift to lower or higher frequency indicating towards the dynamical movement of a Compton cloud or an inner disk front. Both eastern and western approaches were used to unfold the X-ray continuum and systematic changes were observed in soft and hard X-ray spectral components. Simultaneous energy spectral and power density spectral study shows that the production of HBOs is closely related to the Comptonizing region rather than the accretion disk. We discuss the results in the context of re-condensation of coronal material in the inner accretion disk region.

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Using spatial misalignment Method to Measure and Evaluate unbalanced reginal tourism development in Southwest China

  • Lee, Rui;Kim, Hyung-Ho
    • International Journal of Advanced Culture Technology
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    • v.9 no.3
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    • pp.23-33
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    • 2021
  • "China's Western Development Policy" has brought multiple opportunities to the development of tourism in Southwest China including Sichuan, Guizhou, Yunnan, Chongqing and Tibet. The 4 provinces and 1 municipality overall show a certain degree of accumulation effect and coordinated development in tourism due to their location, traffic and traditional economic cooperation. This study takes the Southwest China as the research object and utilized the spatial dislocation model and the tourism spatial misalignment index to estimate the mismatch degree between tourism resources and tourism income among provinces and try to find out the internal reason background. The results show that each of the five provinces has its own advantages in index of economy, tourism resources, human resource, and transportation, leading to differences in the center of gravity of the entire region in all aspects. In view of the results of spatial dislocation analysis, suggestions for improvement and optimization are put forward to promote the high-quality development of tourism in Southwest region. development.

Between Orientalism and Ornamentalism: Colonial Perceptions of Southeast Asian Rulers: 1850-1914

  • Keck, Stephen
    • SUVANNABHUMI
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    • v.10 no.1
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    • pp.7-34
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    • 2018
  • Finding distinguishing characteristics of Southeast Asia has proven to be a significant challenge: by focusing on the encounters which primarily colonial British writers had with the region's state rulers, it becomes possible to recover the early conceptualizations of regional governance. The writings of Henry Yule, Anna Leonowens, Sir George Scott, and Hugh Clifford all document the "orientalist" features of Western discourses because these writers at once were affected by it as they contributed to it. The discourse about royalty and rulers was central to many of the tropes associated with orientalism, but also with 'ornamentalism'. David Cannadine has shown that ornamentalism (in which British conceptualized many imperial practices in relation to their own hierarchical conceptions of society) was as critical a feature of imperial outlook as was orientalism. The need to understand ruling elites was at the heart of the imperialist project. Tracing the ways in which colonizing powers represented the region's ruling elite offers a new avenue for recognizing the affinities of the regional experience. Beyond orientalism, the paper explores questions about the representation and presentation of authority. Understanding the conceptualizations of rulers is connected to the comprehension of social organization-including representations of "traditional society."

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Gene Editing for Major Allergy Genes using Multiplex CRISPR-Cas9 System & Prime editing in Peanuts (Arachis hypogaea L.)

  • Min-cheol Kim;Tae-Hwan Jun
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.194-194
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    • 2022
  • Recently, food-induced allergies have emerged as major global concerns. In the past ten years, it has doubled in western nations, and it has also increased in Asia and Africa. In many cases of food allergy, peanut allergy is prevalent, typically permanent, and frequently life-threatening. Therefore, we utilized gene editing techniques on the three major allergen genes in peanuts, Ara h 1, Ara h 2, and Ara h 3. Using gibson assembly and golden gate assembly, we created two vectors, the gRNA-tRNA array CRISPR-Cas9 system and Prime-editing. Using LBA4404 strain and agrobacterium-mediated transformation, the vectors were transferred to two elite Korean peanut lines. After co-cultivation and tissue culture, we extracted the tissue cultured peanut DNA amplified the hygromycin resistance gene and Cas9 gene in the T-DNA region. The integration of the T-DNA region into the host genome was demonstrated by the presence of a specific band in some samples. There have only been a few reported peanut gene editing studies. So, this study will contribute to peanut allergy and gene editing research.

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Gene Editing for Major Allergy Genes using Multiplex CRISPR-Cas9 System & Prime Editing in Peanuts (Arachis hypogaea L.)

  • Min-cheol Kim;Tae-Hwan Jun
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.200-200
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    • 2022
  • Recently, food-induced allergies have emerged as major global concerns. In the past ten years, it has doubled in western nations, and it has also increased in Asia and Africa. In many cases of food allergy, peanut allergy is prevalent, typically permanent, and frequently life-threatening. Therefore, we utilized gene editing techniques on the three major allergen genes in peanuts, Ara h 1, Ara h 2, and Ara h 3. Using gibson assembly and golden gate assembly, we created two vectors, the gRNA-tRNA array CRISPR-Cas9 system and Prime-editing. Using LBA4404 strain and agrobacterium-mediated transformation, the vectors were transferred to two elite Korean peanut lines. After co-cultivation and tissue culture, we extracted the tissue cultured peanut DNA amplified the hygromycin resistance gene and Cas9 gene in the T-DNA region. The integration of the T-DNA region into the host genome was demonstrated by the presence of a specific band in some samples. There have only been a few reported peanut gene editing studies. So, this study will contribute to peanut allergy and gene editing research.

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A new species of the genus Monoblastus Hartig (Hymenoptera: Ichneumonidae: Tryphoninae) with a key to species from South Korea

  • Jin-Kyung Choi
    • Journal of Species Research
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    • v.12 no.4
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    • pp.321-325
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    • 2023
  • A new species, Monoblastus forsythia sp. nov. is described from South Korea. All specimens of this new species were collected on Forsythia koreana (Nakai). A taxonomic study of South Korean Monoblastus was initiated by Uchida in 1930, and until now four species have been recorded from South Korea. The genus Monoblastus is a Holarctic genus with 31 described species, 14 species are Nearctic, seven are Western Palaearctic region, and nine species occur in Eastern Palaearctic region. In the study, I provide description and photos of both sexes of this new species. Also, modified key to species of the South Korean Monoblastus is provided.