• 자원ㆍ환경경제연구
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• 제15권4호
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• pp.761-782
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• 2006

미국의 농업경영, 특히 축산업으로 인한 잠재적인 환경피해는 많은 우려를 낳고 있다. 축산업간에도 종별로 생산과정과 축산폐수관리 과정 등이 다르고 특히 각기 다른 산업별 구조변화로 환경정책의 영향은 각각 다를 것이다. 비육우의 경우 전통적으로 강한 주들이 지금도 주요한 생산지이지만 양돈의 경우에는 규모별 입지가 급격히 변해왔고 이 과정에서 환경정책에 대한 반영이 비육우의 경우보다 더욱 능동적이었을 것으로 사료된다. 본 연구는 이 같은 가정 하에 양돈업이 축산규제로부터 더 강한 영향을 받을 뿐 아니라 더 많은 영향을 줄 것이라는 가설을 세웠다. 결과를 보면 주 정부의 집행의사가 없는 규제정책은 큰 효율성이 없는 것으로 나타났다. 반면 집행의지는 상당한 영향을 주는 것으로 분석되었다. 그러나 양돈업과 같이 내부적인 구조변화가 심하게 진행되고 있는 경우가 양돈업의 규모와 입지선정에 보다 민감함을 보였다. 환경순응비는 전체 생산비 중 극히 일부분에 불과하다. 따라서 육우산업의 경우에는 높은 고정비 때문에 환경순응에 상대적으로 덜 민감한 반면 양돈업의 경우에는 구조변화 과정에서 환경순응비를 고려한 위치와 규모를 민감하게 반영하고 있는 것으로 판단된다. 미국의 많은 주들이 양돈을 대상으로 한 새로운 규제를 시행하고 있는 점도 이를 시사하는 것이다.

• 분석과학
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• 제26권4호
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• pp.276-286
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• 2013

국내 유해폐기물의 적정 관리를 위해 폐유 및 액상연료 공정에서 발생한 폐기물 중 규제 무기물질류의 배출특성을 조사하였다. 사업장은 올바로시스템에 등록된 폐기물 배출업체를 대상으로, 유럽폐기물 분류체계(EWC, European Waste Catalogue)의 폐유 및 액상연료 공정(EWC 13)과 유사한 폐기물 발생업체를 선정하였다. 조사대상 사업장 37 개 업체를 현지 방문하여 원료, 생산제품, 생산 공정, 폐기물의 종류 및 배출과정을 조사하고 생산 공정에서 배출되는 51 개의 폐기물을 채취하여 무기물질류 16 항목(Cd, Pb, Cu, Hg, As, CN, Cr, $Cr^{6+}$, Ba, Be, F, Ni, Sb, Se, V, Zn)에 대한 함량분석을 수행하였다. 분석결과, 모든 공정에서 발생된 폐기물에서 주로 Sb가 검출되었고, 6~419(평균 98) mg/kg의 농도 범위를 나타내었다. EWC 판정 절차를 통해 국내 폐유 및 액상연료 공정에서 발생된 폐기물을 판정한 결과, EWC 중분류 24공정 중 절대유해폐기물로 분류해서 관리해야할 공정은 16 개, 상대유해폐기물로 관리가 필요한 공정은 8 개로 판단되었다. 폐기물의 배출특성을 통해 유해물질 함유 자료를 확보하고 폐기물 목록에 대한 데이터베이스를 구축함으로써 유해폐기물의 안전처리를 통한 환경오염을 방지할 수 있을 것으로 기대된다.

• 한국산학기술학회논문지
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• 제19권2호
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• pp.347-356
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• 2018

본 연구는 보육교사의 스트레스 수준, 건강상태가 소진에 미치는 영향을 파악하기 위하여 시도되었다. 연구 대상자는 시 소재 보육시설에서 일하고 있는 보육교사 236명을 대상으로 자료 수집을 실시하여 이중 부적절한 응답을 제외한 총 220부를 분석하였다. 자료분석은 SPSS/WIN 21.0 program을 이용하여 통계 처리하였다. 연구 결과 보육교사의 인구사회학적 특성에 따른 스트레스 수준, 건강상태, 소진과의 관련 요인은 연령, 자녀유무, 1일 평균 근무시간, 주관적 건강상태가 관련이 있는 것으로 파악되었다. 보육교사의 스트레스 수준, 건강상태 및 소진 간의 관계에서 소진은 스트레스 수준과 통계적으로 유의한 양의 상관관계를 보였으며, 건강상태와는 통계적으로 유의한 음의 상관관계를 나타냈다. 보육교사의 스트레스 수준과 건강상태가 소진에 미치는 영향에 대한 본 연구의 회귀모형은 통계적으로 적합하였으며(F=12.769, p<.001), 전체 모형의 설명력은 47.6%(Adjusted $R^2=.476$)로, 심리적 건강(${\beta}=-.297$, p<.001), 사회적 건강(${\beta}=-.257$, p<.001), 스트레스 수준(${\beta}=.247$, p<.001) 순으로 나타났다. 따라서 보육교사의 소진을 낮추기 위해서는 심리적, 사회적 건강 프로그램을 적용하고, 스트레스 감소를 위한 지속적인 관리프로그램 개발과 정부 및 민간 차원의 다양한 제도 개선과 대책방안 마련이 필요할 것으로 사료된다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권3호
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• pp.1099-1104
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• 2014

Aims and Background: Ginsenoside Rh2, which exerts the potent anticancer action both in vitro and in vivo, is one of the most well characterized ginsenosides extracted from ginseng. Although its effects on cancer are significant, the underlying mechanisms remain unknown. In this study, we sought to elucidate possible links between ginsenoside Rh2 and phosphoglucose isomerase/autocrine motility factor (PGI/AMF). Methods: $KG1{\alpha}$, a leukemia cell line highly expressing PGI/AMF was assessed by western blot analysis and reverse transcription- PCR (RT-PCR) assay after transfection of a small interfering (si)-RNA to silence PGI/AMF. The effect of PGI/AMF on proliferation was measured by typan blue assay and antibody array. A cell counting kit (CCK)-8 and flow cytometry (FCM) were adopted to investigate the effects of Rh2 on PGI/AMF. The relationships between PGI/AMF and Rh2 associated with Akt, mTOR, Raptor, Rag were detected by western blot analysis. Results: KG1${\alpha}$ cells expressed PGI/AMF and its down-regulation significantly inhibited proliferation. The antibody array indicated that the probable mechanism was reduced expression of PARP, State1, SAPK/JNK and Erk1/2, while those of PRAS40 and p38 were up-regulated. Silencing of PGI/AMF enhanced the sensibility of $KG1{\alpha}$ to Rh2 by suppressing the expression of mTOR, Raptor and Akt. Conclusion: These results suggested that ginsenoside Rh2 suppressed the proliferation of $KG1{\alpha}$, the same as down-regulation of PGI/AMF. Down-regulation of PGI/AMF enhanced the pharmacological effects of ginsenoside Rh2 on KG1${\alpha}$ by reducing Akt/mTOR signaling.

• 기술혁신학회지
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• 제10권3호
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• pp.406-430
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• 2007

본고는 제약분야 라이센싱 거래의 기초가 되는 주요 경제적 조건에 근거한 분석적 접근방법을 검토하기 위한 것이다. 그 목적은 관련된 주요 요인들 간의 관계에 대한 이해를 높이는 것이다. 여기에서 제기되는 몇 가지 논점은 다음과 같다. 즉, 신약개발의 특징인 대규모 투자, 고위험 및 긴 개발기간 등의 수익에 대한 효과, 최종 매출수준과 영업이익이 적정 로열티율에 미치는 효과, 선불금 및 신약개발 단계별로 지불될 마일스톤 기술료와 약품이 시장에 진입된 후에 지불될 기술료간의 관계 등이 그것이다. 본고에서는 먼저 제약분야 기술이전 거래의 로열티 결정을 위한 사전 단계로서, 기술가치평가의 주요 방법과 특징을 살펴보고, 다음으로 로열티 결정을 위한 주요요소인 기대이익, 매출액, 각종 비용, 투자 등의 측정방법을 검토한다. 그리고 이러한 주요 요소의 측정에 기초하여 로열티와 이윤율, 기술제공자와 기술도입자 간의 이익배분, 로열티의 산출과 결정방식에 대해 고찰하고자 한다.

• 대한약침학회지
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• 제15권3호
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• pp.13-18
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• 2012

Objectives: The purpose of this study is to examine whether Hominis Placental pharmacopuncture solution (HPPS) combined with zinc-oxide nanoparticles (ZnO NP) activates RAW 264.7 cells. Methods: We soaked ZnO nanoparticles in the Hominis Placenta pharmacopuncture solution, thereby making a combined form (ZnO NP HPPS). The effect of ZnO NP HPPS on the intracellular reactive oxygen species (ROS) production was measured by 2', 7'-dichlorofluorescin diacetate (DCFH-DA) assay. The effect of ZnO NP HPPS on NF-${\kappa}B$ was measured by using a luciferase assay. The effect of ZnO NP HPPS on the cytokine expression was assessed by semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR). The cellular uptake of ZnO NP HPPS was measured by using a flow cytometric analysis, and cellular structural alterations were analyzed by using transmission electron microscopy (TEM). Results: Neither the HPPS nor the ZnO NPs induced intracellular ROS production in RAW 264.7 cells. Neither of the materials activated NF-${\kappa}B$ or it's dependent genes, such as TNF-${\alpha}$, IL-1, and MCP-1. However, ZnO NP HPPS, the combined form of ZnO NPs and HPPS, did induce the intracellular ROS production, as well as prominently activating NF-${\kappa}B$ and it's dependent genes. Also, compared to ZnO NPs, it effectively increa-sed the uptake by RAW 264.7 cells. In addition, cellular structural alterations were observed in groups treated with ZnO NP HPPS. Conclusions: Neither ZnO NP nor HPPS activated RAW 264.7 cells, which is likely due to a low cellular uptake. The ZnO NP HPPS, however, significantly activated NF-${\kappa}B$ and up-regulated its dependent genes such as TNF-${\alpha}$, IL-1, and MCP-1. ZnO NP HPPS was also more easily taken into the RAW 264.7 cells than either ZnO NP or HPPS.

• 유통과학연구
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• 제12권11호
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• pp.55-65
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• 2014

Purpose - This study aims to investigate the process of political changes in Japan, which has introduced regulatory policies for large-scale retail stores since the 1930s, as well as the examples, and suggests improvement schemes for our policies in Korea, which imposes restrictions on business hours and forced holidays in accordance with the current Distribution Industry Development Act. Research design, data, and methodology - After examining the political change processes related to large-scale retail stores in japan, this study analyzes individually regulated cases based on the ordinances enacted by each local government. Through case analysis in Japan, this study makes political suggestions that may be helpful for our country substantially. Results - Since there is an obvious possibility that our economic restrictions on business hours and mandatory holidays do not coincide with WTO GATS, it is necessary for large-scale distributors to introduce new social and environmental regulations similar to Japan, rather than imposing controls to restrict free competition and also introduce a policy to induce cooperation with small businesses for the advancement of the distribution industry. Thus, it is desirable to take measures on noise, waste, traffic, and parking for the preservation of the living environment in the surroundings when building new large-scale retail stores. It is also important to establish measures to improve the welfare of neighborhood residents and consumers, create a pleasant urban environment, and make it mandatory to make presentations at public hearings among residents. Furthermore, it should be mandatory to establish regional contribution plans when a retail store is established, and take measures to solve various civil complaints or problems that may occur after entering the market. Moreover, it is desirable for large-scale retail stores that entered the market to induce cooperation in performing various activities in the area with a strong sense that they are all members of the local economy. Conclusions - If introducing social regulations like in Japan, there is probably an advantage that the conflicts seen when large-scale retail stores enter the market are absorbed by adjusting the persons concerned within the established institution in order to establish a field to solve such conflicts systematically. In contrast, there are still concerns regarding chaotic operation without any active attempts to have a conversation with large-scale retail stores and local small merchants due to a sharp conflict among the persons concerned, and if it is a briefing session without any decision of the restrictions on their opening itself, there may be doubts with regard to their effectiveness. Moreover, if the de facto opening is restricted by the introduction of such a briefing session procedure, the choice of whether to protect the existing rights of large-scale retail stores might become problematic. However, such problems could be minimized in a way by forming a separate consultative group for all persons concerned including residents, local governments, professionals, civic organizations, small merchants, and massive retail store-related persons.

• Journal of Microbiology and Biotechnology
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• 제30권10호
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• pp.1458-1466
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• 2020

Oligomeric proanthocyanidins (OPCs), classified as condensed tannins, have significant antioxidation, anti-inflammation and anti-cancer effects. This study was performed to investigate the anti-inflammatory effects of OPCs and the mechanism underlying these effects in lipopolysaccharide (LPS)-stimulated bovine mammary epithelial cells (MAC-T). Real-time PCR and ELISA assays indicated that OPC treatment at 1, 3 and 5 ㎍/ml significantly reduced the mRNA and protein, respectively, of oxidant indicators cyclooxygenase-2 (COX-2) (p < 0.05) and inducible nitric oxide synthase (iNOS) (p < 0.01) as well as inflammation cytokines interleukin (IL)-6 (p < 0.01), IL-1β (p < 0.01) and tumor necrosis factor-α (TNF-α) (p < 0.05) in LPS-induced MAC-T cells. Moreover, OPCs downregulated LPS-induced phosphorylation of p65 and inhibitor of nuclear factor kappa B (NF-κB) (IκB) in the NF-κB signaling pathway (p < 0.01), and they inhibited p65 translocation from the cytoplasm to the nucleus as revealed by immunofluorescence test and western blot. Additionally, OPCs decreased phosphorylation of p38, extracellular signal regulated kinase and c-jun NH₂-terminal kinase in the MAPK signaling pathway (p < 0.01). In conclusion, the anti-inflammatory and antioxidant activities of OPCs involve NF-κB and MAPK signaling pathways, thus inhibiting expression of pro-inflammatory factors and oxidation indicators. These findings provide novel experimental evidence for the further practical application of OPCs in prevention and treatment of bovine mastitis.

• The Korean Journal of Physiology and Pharmacology
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• 제6권6호
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• pp.305-309
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• 2002

There are some evidences that $K^+$ efflux evoked by muscarinic stimulation is not mainly mediated by large conductance $K^+$ (BK) channels in salivary gland. In this experiment, we therefore characterised non BK channels in rat submandibular gland acinar cells and examined the possibility of agonist effect on this channel using a patch clamp technique. Two types of $K^+$ channels were observed in these cells. BK channels were observed in 3 cells from total 6 cells and its average conductance was $152{\pm}7$ pS (n=3). The conductance of the another types of $K^+$ channel was estimated as $71{\pm}7$ pS (n=6). On the basis of the conductance of this channel, we defined this channel as intermediate conductance $K^+$ (IK) channels, which were observed from all 6 cells we studied. When we increased $Ca^{2+}$ concentration of the bath solution in inside-out mode, the IK channel activity was greatly increased, suggesting this channel is $Ca^{2+}$ sensitive. We next examined the effect of carbachol (CCh) and isoproterenol on the activity of the IK channels. $10^{-5}$ M isoproterenol significantly increased the open probability (Po) from $0.08{\pm}0.02$ to $0.21{\pm}0.03$ (n=4, P<0.05). Application of $10^{-5}$ M CCh also increased Po from $0.048{\pm}0.03$ to $0.55{\pm}0.33$ (n=5, P<0.05) at the maximum channel activity. The degree of BK channel activation induced by the same concentration of CCh was lower than that of IK channels; Po value was $0.011{\pm}0.003$ and $0.027{\pm}0.005$ in control and during CCh stimulation (n=3), respectively. The result suggests that IK channels exist in salivary acinar cells and its channel activity is regulated by muscaricinic and ${\beta}-adrenergic$ agonist. We conclude that IK channels also play a putative role in secretion as well as the BK channels in rat submandibular gland acinar cells.

• 약학회지
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• 제53권5호
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• pp.286-292
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• 2009

In this study, we investigated the anti-obese activity of HPJ extract in C57BL/6J mice. The C57BL/6J mice were randomly divided into five groups: normal control group (Con), high fat diet control group (HFD), treatment groups with HPJ at 125 mg/kg (HPJ125), 250 mg/kg (HPJ250), or 500 mg/kg (HPJ500). To induce an obesity, mice were fed by a high fat diet for 6 weeks, and mice were administered with HPJ extract once a day for 8 weeks. At the end of treatment, we examined the effect of HPJ extract on body weight, plasma lipid, and lipogenic enzymes. HPJ extract was found to lower whole body and epididymal adipose tissue weights and lowered plasma levels of glucose, insulin, triglyceride (TG), total cholesterol (TC), non-esterified fatty acid (NEFA) and leptin, compared to those in HFD group. Histological analyses of the liver and fat tissues of mice treated with HPJ extract revealed significantly decreased number of lipid droplets and decreased size of adipocytes compared to the HFD group. In addition, HPJ extract preserved the morphological integrity of pancreatic islets. To elucidate an action mechanism of HPJ extract, Western blot and RT-PCR were performed using epididymal adipose tissues. HPJ extract up-regulated the levels of phosphorylated adenosine monophosphate-activated protein kinase (AMPK) and its substrate, acetyl-CoA carboxylasse (ACC). HPJ extract also attenuated lipogenic gene expressions of sterol regulatory element-binding protein $1{\alpha}$ (SREBP$1{\alpha}$), fatty acid synthase (FAS), sterol-CoA desaturase 1 (SCD1) and glycerol-3-phosphate acyltransferase (GPAT) in dose-dependent manners. In contrast, expressions of lipolytic genes such as peroxisome proliferator-activated receptor-$\alpha$ (PPAR-${\alpha}$) and CD36, and fatty acid $\beta$-oxidation gene, carnitine palmitoyltransferase-1 (CPT-1) were increased. These results suggest that HPJ extract ameliorates obesity through inhibiting synthesis of lipogenic enzymes as well as stimulating fatty acid oxidation resulting from activation of AMPK, and HPJ extract could be developed as a potential therapeutic agent for obese patients.