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Rapid Staining Using the Shorr Method for Intraoperative Peritoneal Washing Cytology in Advanced Gastric Cancer: a Pilot Study from a Single Institution

  • Son, Sang-Yong;Choi, Hai-Young;Lee, Yoontaek;Park, Young Suk;Shin, Dong Joon;Oo, Aung Myint;Jung, Do Hyun;Ahn, Sang-Hoon;Park, Do Joong;Lee, Hye Seung;Kim, Hyung-Ho
    • Journal of Gastric Cancer
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    • v.19 no.2
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    • pp.173-182
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    • 2019
  • Purpose: Intraoperative peritoneal washing cytology (PWC) is used to determine treatment strategies for gastric cancer with suspected serosal invasion. However, a standard staining method for intraoperative PWC remains to be established. We evaluated the feasibility of a rapid and simple staining method using Shorr's stain for intraoperative PWC in advanced gastric cancer. Materials and Methods: Between November 2012 and December 2014, 77 patients with clinical T3 or higher gastric cancer were enrolled. The sensitivity, specificity, and concordance between the Shorr staining method and conventional Papanicolaou (Pap) staining with carcinoembryonic antigen (CEA) immunohistochemistry (IHC) were analyzed. Results: Intraoperative PWC was performed laparoscopically in 69 patients (89.6%). The average time of the procedure was 8.3 minutes, and the average amount of aspirated fluids was 83.3 mL. The average time for Shorr staining and pathologic review was 21.0 minutes. Of the 77 patients, 16 (20.7%) had positive cytology and 7 (9.1%) showed atypical findings; sensitivity and specificity were 73.6% and 98.2% for the Shorr method, and 78.9% and 98.2% for the Pap method with CEA IHC, respectively. Concordance of diagnosis between the 2 methods was observed in 90.9% of cases (weighted ${\kappa}$ statistic=0.875) and most disagreements in diagnoses occurred in atypical findings (6/7). In overall survival, there was no significant difference in C-index between the 2 methods (0.459 in Shorr method vs. 0.458 in Pap with CEA IHC method, P=0.987). Conclusions: Shorr staining could be a rapid and reliable method for intraoperative PWC in advanced gastric cancer.

A Pre-Feasibility Test of Introducing Renewable Energy Hybrid Systems -Case Studies for 3 Off-Grid Islands- (도서지역 신·재생복합 전력시스템 보급 타당성 분석 -3개 도서지역 분석결과-)

  • Jang, HaNa;Kim, Suduk
    • Environmental and Resource Economics Review
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    • v.15 no.4
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    • pp.693-712
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    • 2006
  • A pre-feasibility test is done for renewable energy hybrid power systems at off~grid islands in which the current power supply is provided only by diesel generation. We apply Homer (Hybrid Optimization Model for Electric Renewables) which was developed by the National Renewable Energy Laboratory (NREL) for the analysis to identify the cost-minimizing combination of power generating facilities for the given load profiles. Chuja-Do, Geomun-Do and Youngsan-Do have been selected for our analysis considering the wind resources data of the Korea Institute of Energy Research (KIER). Information on wind speed, solar radiation and temperature is also used for the analysis. System component cost information from overseas market has been used due to the lack of domestic information. Site specific Load profile for electricity demand for those islands are reconstructed based on the partial survey results obtained form other sources. The LCOE of the least cost hybrid power systems for Chuja-Do, Geomun-Do and Youngsan-Do are $0.278/kWh, $0.234/kWh and $0.353/kWh, respectively Considering the fact that diesel generation is being subsidized at the price of $0.300/kWh by the government, first 2 cases are economically feasible for the introduction of renewable energy hybrid systems to those islands. But the third case of Youngsan-Do does not meet the criteria. The basic differences of these pre-feasibility test results are from the differences of the site specific renewable energy conditions, especially wind resources. In summary, promoting hybrid systems in the off-grid remote island should be based on the economic feasibility test results. Not all the off-grid islands are feasible for introducing this renewable energy hybrid system.

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Development of Isolation and Cultivation Method for Outer Root Sheath Cells from Human Hair Follicle and Construction of Bioartificial Skin

  • Sin, Yeon-Ho;Seo, Yeong-Gwon;Lee, Du-Hun;Yu, Bo-Yeong;Song, Gye-Yong;Seo, Seong-Jun;Hwang, Seong-Ju;Kim, Yeong-Jin;Yang, Eun-Gyeong;Park, Jang-Seo;Jang, Lee-Seop;Park, Jeong-Geuk
    • 한국생물공학회:학술대회논문집
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    • 2003.04a
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    • pp.302-305
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    • 2003
  • It is difficult to obtain sufficient healthy skin for coverage of a wide area of skin wound. In the skin, an additional population of living epithelial cells is located in the outer root sheath (ORS) of hair $follicles.^{1),2)}$ ORS cells should be a good source of epithelium because they are easily obtainable and patients do not have to suffer from scar formation at donor sites. We modified ordinary primary culture technique for the purpose of solving such problem that epithelial cells have a low propagation and easy aging during culture periods. First of all, we improved primary cultivation methods. In the ordinary primary culture, average yield of human ORS cells was $2\;{\times}\;10^3$ cells/follicle by direct incubation with trypsin (0.1%)/EDTA (0.02%) solution for 15 min at $37^{\circ}C$ but we could obtain about $6.5\;{\times}\;10^3$ cells/follicle by two step enzyme digestion method with dispase (1.2 U/ml) and trypsin (0.1%)/EDTA (0.02%) solution. So we could achieve three times higher primary cultured ORS cell yield. Secondly, we could obtain total $2\;{\times}\;10^7$ cells in serum free medium and even more total $6\;{\times}\;10^7$ cells in modified E-medium with mitomycin C-treated feeder cells during 17 days. Using the cultured ORS cells, and we could make bioartificial skin equivalent in vitro and concluded that ORS cells were progenitor cells for skin epithelial cell.

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A Design of Predistortion Linearizer using 2nd Low Frequency Intermodulation Signal Injection (2차 저주파 혼변조 신호 주입을 이용한 전치 왜곡 선형 화기 설계)

  • Lee, Hyo-A;Lee, Chul-Whan;Jeong, Yong-Chae;Kim, Young;Kim, Chul-Dong
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.14 no.9
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    • pp.967-973
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    • 2003
  • This paper presents a new predistortion method which injects the 2nd low-frequency intermodulation signal of RF signals into the input bias line of the amplifier. New 2nd intermodulation signal extraction circuit is also proposed. We have shown that this method can suppress the 3rd IM apparently and sometimes do the 5th IM, through mathematical analysis, then confirmed it with simulation and verified it on the desk test. When the input signal CDMA IS-95 lFA is applied, measured ACPR improvements are 25 dBc, 22.5 dBc, and 6 dBc at 0.885 MHz, l.25 MHz and 2.25 MHz offset respectively. Also, when applying the CDMA IS-95 3FA, the measured ACPR improvement is 20 dBc at 0.885 MHz offset.

Estimated Headwater Stream Temperature Using Environmental Factors with Seasonal Variations in a Forested Catchment (환경인자를 이용한 산지계류의 계절별 수온변화 예측)

  • Nam, Sooyoun;Jang, Su-Jin;Kim, Suk-Woo;Lee, Youn-Tae;Chun, Kun-Woo
    • Korean Journal of Environment and Ecology
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    • v.34 no.1
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    • pp.55-62
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    • 2020
  • To estimate headwater stream temperature with seasonal variations, we analyzed precipitation, runoff and air temperature in experimental forest of Kangwon National University, Gangwon-do (2017~2018 years). The daily mean value of headwater stream temperature for spring was 6.9~17.7℃ and correlated with air temperature, that for summer and fall were 12.2~26.3℃ and 3.6~19.3℃, correlated with air temperature and runoff. Based on seasonal variations, we applied for stepwise multiple linear regression analyses to estimate headwater stream temperature with seasonal variations. The equations were headwater stream temperature(WT)spring=(0.553×Air temperature)+(0.086×Runoff)+4.145 (R2=0.505; p<0.01), WTsummer=(0.756×Air temperature)+(-0.072×Runoff)+2.670 (R2=0.510; p<0.01), and WTfall=(0.738×Air temperature)+(0.028×Precipitation)+2.660 (R2=0.844; p<0.01). The coefficient of determination (R2) was greater than when it was estimated by air temperature in all seasons and progressively increased from spring to winter. Therefore, we indicated difference on estimated magnitude of stepwise multiple linear regression, due to effects on headwater stream temperature of different environmental factors with seasonal variations. Furthermore, temporal factors with spatial characteristics (e.g., river versus headwater stream) could be recommended for estimating headwater stream temperature.

Micropropagation of Aronia (Aronia melaocarpa Elliot, black chokeberry) and its 5 varieties (아로니아(Aronia melanocarpa Elliot) 5개 품종의 기내번식)

  • Kwak, Myoung-Chul;Choi, Chung-Ho;Choi, Yong-Eui;Moon, Heung-Kyu
    • Journal of Plant Biotechnology
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    • v.42 no.4
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    • pp.380-387
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    • 2015
  • Aronia (Aronia melanocarpa, Black chokeberry) is an important cash crop in domestic agriculture. We investigated the effects of plant growth regulators on shoot proliferation and rooting using in vitro tissue culture. The most effective shoot multiplication was observed on WPM (woody plant medium) supplemented with 1.0 mg/L zeatin ($8.3{\pm}1.0$ shoots/explant), while the highest rooting rate was obtained from half-strength WPM with 3.0 mg/L IBA (8.8 roots/explant). The rooted plantlets all survived in the artificial soil mixture (with a mixture of peat moss : perlite : vermiculite, 1:1:1, v/v/v) and grew up relatively uniform, ranging from 14 to 16 leaves, 8 to 10 cm in stem height, and 2.3 to 2.8 mm in stem diameter. While experimenting with 5 different varieties of Aronia, we found out that each variety had different characteristics of shoot proliferation and rooting. The total numbers of proliferated shoots per variety is as follows: $17.4{\pm}0.8$ for Nero, 14 to 15 for Purple and Mackenzie, and 10 for both Viking and Odamamachiko. Rooting rates were also various depending on the variety: 88% of Odamamachiko, 80% of Viking and Purple, and 76% of Nero and 60% of Mackenzie shoots rooted. The survival rate of the rooted plantlets was from 92% to 100%, varying by type. Further growth appeared to be better in auxin-treated plantlets, compared to untreated ones. Our results showed the possibility of establishing an effective in vitro micropropagation system for Aronia melanocarpa.

Prehospital care after return of spontaneous circulation in out-of-hospital cardiac arrest patients: Based on Heart Saver laureate (병원 전 심정지 환자의 자발순환 회복에 관한 병원전 처치 - 하트세이버 수상자를 중심으로 -)

  • Koh, Bong-Yeun;Hong, Sung-Gi;Kim, Jin-Young
    • The Korean Journal of Emergency Medical Services
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    • v.18 no.2
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    • pp.125-136
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    • 2014
  • Purpose: We aimed to improve the survival rates of out-of-hospital cardiac arrest patients. Methods: We analyzed data regarding cardiopulmonary resuscitation (CPR) outcomes and clinical characteristics of out-of-hospital cardiac arrest patients. The data included prehospital emergency medical service reports of 207 patients, 135 patients of Heart Saver, who survived over 72 hours after return of spontaneous circulation (ROSC) in Gyeonggi-do from January, 2012 to December, 2013. Data were analyzed using SPSS 18.0 descriptive statistics. Results: Among patients who achieved ROSC, 87.6% were men and 73.6% were aged 41-70 years; 86.7% were cases of witnessed cardiac arrest, and cardiopulmonary resuscitation was performed by bystanders in 65.9% of cases. The initial electrocardiogram showed ventricular fibrillation or pulseless ventricular tachycardia in 96.3% of patients. The call time was 1.0 minutes, arrival time was 6.3 minutes, time spent at the scene was 8.0 minutes, hospital arrival time was 10.0 minutes, and total CPR duration was 9.6 minutes. The certificate of them was paramedics in 89.6%. Conclusion: To improve the survival rates of out-of-hospital cardiac arrest patients, standard prehospital care for these patients and educational programs regarding CPR for lay rescues should be developed.

Landform and Drainage Analysis in Geoje-Do Using GIS (GIS를 이용한 거제도 지형 및 하계 분석)

  • Kim, Woo-Kwan;Lim, Yong-Ho
    • Journal of the Korean association of regional geographers
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    • v.3 no.2
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    • pp.19-35
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    • 1997
  • The purpose of this study is to find out the characteristics of landform in Geoje-Do using GIS and DTED data. The characteristics of landform in Geoje-Do are as follows; First, the height-range of Geoje-Do is $0{\sim}580m$, and the average elevation of it is 124m. Volcanic and granite region is mainly appeared at high elevation-region. But, we can't find out outstanding difference of elevation, according to its geology. The second. the slope-range of Geoje-Do is $0{\sim}52$ degree, and the average slope of it is 17.6 degree. The slope of volcanic and granite area is more steeper than any other region. But the results of analysis of the geology in Geojo-Do, don't show outstanding difference of the slope. The third, the area-rate of the aspect of Geoje-Do is almost same in all direction. And the area-rate of south-west direction is the highest. According to the geology of Geoje-Do, granite is distributed the most widely, and the area of volcanic and granite occupy 60% of entire island's area. According to analysis of influence of geology with elevation, geology has little relationship with elevation. According to analysis of geology and drainage network, streams are inclined to be developed well in Alluvium area. Drainage network is well developed throughout the entire island, except southeast area. The highest order of stream is 4 in 1:25,000 topographic map. The density of stream in Geoje-Do is very high, such as 1.6. The bifurcation-ratio of stream is also higher than 4 in all order. The length-ratio of stream is ranged from 1.24 to 3.25. According to the relationship between order and elevation. order is the greater, elevation is the lower. According to the relationship between order and slope, order is the greater, slope is the gentler. In this study, we use DTED Data, and compare it with topographic map data. According to the comparison, there is a little difference between DTED data and topographic map data. Therefore, to use DTED data in landform analysis, it is required coordinate matching process. This process is very important, and take very long time. Thus, if you use DTED in landform analysis, some processes are required. DTED data can be taken very easily, but its using is not simple. Because coordinate adjust is very hard work.

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Two Cases of Human Thelaziasis and Brief Review of Korean cases

  • Sohn, Woon-Mok;Na, Byoung-Kuk;Yoo, Ji-Myong
    • Parasites, Hosts and Diseases
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    • v.49 no.3
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    • pp.265-271
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    • 2011
  • The present study was performed to describe 2 cases of human thelaziasis (HT) which occurred in Gyeongsangnam-do and to briefly review the previously reported Korean cases. A 58-year old woman, residing in Hadong-gun, Gyeongsangnam-do, came to Gyeongsang National University Hospital (GNUH) complaining of foreign body sensation and itching of the right eye in March 2000. Total 6 adult nematodes of Thelazia callipaeda (2 males and 4 females) were detected in her right eye. A 80-year old man, residing in Jinju-si, Gyeongsangnam-do, came to GNUH complaining of foreign body sensation, itching, and pain of the right eye in December 2007. A total of 5 worms (4 females and 1 degenerated) were removed from his right eye. We analyzed characteristics of the total 39 Korean HT cases reported to date, including the present 2 cases. Most of the cases (71.8%) occurred in Seoul and Gyeonggi-do before 2000, and 21 cases (53.8%) were males and 18 (46.2%) were females. The prevalence was higher in younger ages below 30 years (48.7%) than 31-60 years (41.0%) and over 61 years (10.3%). The seasonal prevalence showed a higher incidence in autumn (43.6%) than in other seasons. Most of the cases (94.9%) were conjunctival sac infections and only 2 (5.1%) were intraocular cases. The present 2 HT cases are the first reported cases in Gyeongsangnam-do. Some characteristics of Korean HT cases were analyzed.

Effects of Ni additions on Graphitization in High carbon steel (고탄소강의 흑연화에 미치는 Ni첨가의 영향)

  • Park, Young-Koo;Woo, Kee-Do;Jin, Young-Chul;Ryu, Jae-Hwa;Park, Jong-Hyun
    • Journal of the Korean Society for Heat Treatment
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    • v.12 no.1
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    • pp.55-65
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    • 1999
  • Graphitization of cementite in high carbon steels at subcritical temperature is reported. We have studied about the effects of Ni in these steels on graphitization. The chemical compositions of the specimens were Fe-(0.54, 0.7)%-C-1.0%Si-0.1%Mn-(0.2~1.0)%Ni. After annealing at $650^{\circ}C$, $680^{\circ}C$ and $700^{\circ}C$ during various time the microstructures and hardness change were observed. In order to recover the initial hardness of high carbon steel, dissolution treatment of graphite was performed at $870^{\circ}C$. In case of 0.7%C steel, graphitization was accelerated rather 1.0%Ni addition steel than 0.2% and 0.54%Ni addition steels but the graphite is coarser. In case of 0.54%C-0.2%Ni steel, graphite particles were distributed relatively homogeniously and finely. Nickel addition promotes graphitization of these steels but makes graphite blocky.

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