• Journal of Microbiology and Biotechnology
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• v.22 no.9
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• pp.1193-1201
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• 2012

The analysis and quantification of ammonia-oxidizing bacteria (AOB) is crucial, as they initiate the biological removal of ammonia-nitrogen from sewage. Previous methods for analyzing the microbial community structure, which involve the plating of samples or culture media over agar plates, have been inadequate because many microorganisms found in a sewage plant are unculturable. In this study, to exclusively detect AOB, the analysis was carried out via denaturing gradient gel electrophoresis using a primer specific to the amoA gene, which is one of the functional genes known as ammonia monooxygenase. An AOB consortium (S1 sample) that could oxidize an unprecedented 100% of ammonia in 24 h was obtained from sewage sludge. In addition, real-time PCR was used to quantify the AOB. Results of the microbial community analysis in terms of carbon utilization ability of samples showed that the aeration tank water sample (S2), influent water sample (S3), and effluent water sample (S4) used all the 31 substrates considered, whereas the AOB consortium (S1) used only Tween 80, D-galacturonic acid, itaconic acid, D-malic acid, and $_L$-serine after 192 h. The largest concentration of AOB was detected in S1 ($7.6{\times}10^6copies/{\mu}l$), followed by S2 ($3.2{\times}10^6copies/{\mu}l$), S4 ($2.8{\times}10^6copies/{\mu}l$), and S3 ($2.4{\times}10^6copies/{\mu}l$).

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.26 no.3
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• pp.174-183
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• 2014

Seabed beneath and near coastal structures may undergo large excess pore water pressure composed of oscillatory and residual components in the case of long durations of high wave loading. This excess pore water pressure may reduce effective stress and, consequently, the seabed may liquefy. If liquefaction occurs in the seabed, the structure may sink, overturn, and eventually increase the failure potential. In this study, to evaluate the liquefaction potential on the seabed, numerical analysis was conducted using the expanded 2-dimensional numerical wave tank to account for an irregular wave field. In the condition of an irregular wave field, the dynamic wave pressure and water flow velocity acting on the seabed and the surface boundary of the composite breakwater structure were estimated. Simulation results were used as input data in a finite element computer program for elastoplastic seabed response. Simulations evaluated the time and spatial variations in excess pore water pressure, effective stress, and liquefaction potential in the seabed. Additionally, the deformation of the seabed and the displacement of the structure as a function of time were quantitatively evaluated. From the results of the analysis, the liquefaction potential at the seabed in front and rear of the composite breakwater was identified. Since the liquefied seabed particles have no resistance to force, scour potential could increase on the seabed. In addition, the strength decrease of the seabed due to the liquefaction can increase the structural motion and significantly influence the stability of the composite breakwater. Due to limitations of allowable paper length, the studied results were divided into two portions; (I) focusing on the dynamic response of structure, acceleration, deformation of seabed, and (II) focusing on the time variation in excess pore water pressure, liquefaction, effective stress path in the seabed. This paper corresponds to (II).

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.26 no.3
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• pp.160-173
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• 2014

Seabed beneath and near coastal structures may undergo large excess pore water pressure composed of oscillatory and residual components in the case of long durations of high wave loading. This excess pore water pressure may reduce effective stress and, consequently, the seabed may liquefy. If liquefaction occurs in the seabed, the structure may sink, overturn, and eventually increase the failure potential. In this study, to evaluate the liquefaction potential on the seabed, numerical analysis was conducted using the expanded 2-dimensional numerical wave tank to account for an irregular wave field. In the condition of an irregular wave field, the dynamic wave pressure and water flow velocity acting on the seabed and the surface boundary of the composite breakwater structure were estimated. Simulation results were used as input data in a finite element computer program for elastoplastic seabed response. Simulations evaluated the time and spatial variations in excess pore water pressure, effective stress, and liquefaction potential in the seabed. Additionally, the deformation of the seabed and the displacement of the structure as a function of time were quantitatively evaluated. From the results of the analysis, the liquefaction potential at the seabed in front and rear of the composite breakwater was identified. Since the liquefied seabed particles have no resistance to force, scour potential could increase on the seabed. In addition, the strength decrease of the seabed due to the liquefaction can increase the structural motion and significantly influence the stability of the composite breakwater. Due to limitations of allowable paper length, the studied results were divided into two portions; (I) focusing on the dynamic response of structure, acceleration, deformation of seabed, and (II) focusing on the time variation in excess pore water pressure, liquefaction, effective stress path in the seabed. This paper corresponds to (I).

• Journal of Ocean Engineering and Technology
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• v.28 no.5
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• pp.429-439
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• 2014

The target echoes contain information on the target such as the orientation, kinematics, and internal structure, as well as the external geometrical shape of the target. In addition, the pattern of the target echoes depends on the arrangement of the transmitters and receivers in space. Therefore, the study of the target echoes in a multi-static SONAR system can be useful for detecting and tracking submerged objects using an underwater surveillance system. For this purpose, an acoustic measuring system for multi-static target echoes was designed and tested in an acoustic water tank. Some preliminary data are presented and discussed.

• 유체기계공업학회:학술대회논문집
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• 2002.12a
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• pp.419-424
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• 2002

The HANARO, a multi-purpose research reactor of 30 MWth open-tank-in-pool type, has been under normal operation since its initial criticality In February, 1995. Many experiments should be safely performed to activate the utilization of the HANARO. A flow simulation facility is being developed for the endurance test of reactivity control units for extended life times and the verification of structural integrity of those experimental facilities prior to loading in the HANARO. This test facility is composed of three major parts; a half-core structure assembly, flow circulation system and support system. The flow circulation system is composed of a circulation pump, a core flow pipe, a core bypass flow pipe and instruments. The system is to be filled with de-mineralized water and the flow should be met the design flow to simulate similar flow characteristics in the core channel of the half-core test facility to the HANARO. This paper, therefore, describes an analytical analysis to study the flow behavior of the system. The computational flow analysis has been performed for the verification of system pressure variation through the three-dimensional analysis program with standard k-$\epsilon$ turbulence model and for the verification of the structural piping integrity through the finite element method. The results of the analysis are satisfied the design requirements and structural piping integrity of flow circulation system.

• 보존과학연구
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• s.6
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• pp.247-258
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• 1985

Conservation laboratory was attached to Keimyung University Museum inMarch 1980 and ever since it has been devoted mainly to the processing andconservation of metal objects. A number of objects have been processed inthis laboratory during the period, including those already in the collection ofthe Museum, those which were discovered during the three major excavationsof Kaya tombs conducted by the Museum, and those processed on commissionfrom other museums in the country,The activities of this laboratory include: (1) conserving the objects againstfurther erosion; (2) raising the archaeological value of the objects by revealingthe structure of such parts of the objects as concealed under rust; and (3)recovering the original shape of damaged objects.The methods adopted by the laboratory include: (1) removing from theobjects the ionized chlorine which usually are the major cause of erosion; (2)strengthening the objects by soaking them in acrylic resins; and (3) applyingresins to the surface of the objects to protect them from further erosion.Chemicals much employed by the laboratory includes the acrylic resin(Ruschot; developed jointly by the Cultural Property Research Institute ofKorea and Samwha Paint Company), the sodium sesquicarbonate, the sodiumhydroxide, the lithium hydroxide, and the benzotriazole.Major apparatus in the laboratory includes the vacuum immersion tank, theairbrasive, the ultrasonic cleaner, the pH-ion meter, the water bath, the zoomstereo microscope, the drying oven, and the drill.

• Journal of Ocean Engineering and Technology
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• v.22 no.4
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• pp.78-83
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• 2008

In recent years, small boats used for marine leisure have been steadily increasing because of the increase in national income and the desire for marine leisure. But the repair of such small boats in dry dock has pointed out many faults in small FRP-shipbuilding in terms if workspace and manpower. Lifting a boat from the water to land is done with a crane or by hand using a sling around the bottom of the boat. But dry dock work is limited by the scale of the boat, which corresponds to the crane capacity, with carelessness making it possible to capsize a boat and endanger life. The purpose of this study was the development of a marine docking system that would improve economical efficiency and safety, for which we carried out concept design, model tests, structural analysis, etc.

• Geomechanics and Engineering
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• v.14 no.1
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• pp.43-50
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• 2018

Applicability of constructing piled raft foundations on soft clay has been given attention in recent years. Lack of sufficient stiffness for soil and thus excessive settlements to allow higher contribution of piles is the major concern in this regard. This paper presents a numerical investigation of performance of piled-raft foundations on soft clay with focusing on a case study. A 3D FEM numerical model is developed using ABAQUS. The model was calibrated by comparing physical and numerical modeling results of other researchers. Then the possibility of using piled-raft system in construction of foundation for a water storage tank in Sarbandar, Iran is assessed. Soil strength parameters in the numerical model were calibrated using the instrumentation data of a heavily instrumented preloading project at the construction site. The results indicate that choosing the proper combination of length and spacing for piles can lead to acceptable differential and total settlements while a high percentage of total bearing capacity of piles can be mobilized, which is an efficient solution for the project. Overall, the construction of piled-rafts on soft clays is promising as long as the total settlement of the structure is not imposing restrictions such as the common 25 mm allowable settlement. But instead, if higher allowable settlements are adopted, for example in the case of rigid steel tanks, the method shall be applicable with considerable cost savings.

• Journal of the Korean Society of Industry Convergence
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• v.16 no.3
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• pp.95-102
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• 2013

It is indispensable to modern society metal processing since the industrialized rapidly, but it is a metalworking cutting fluid immediately. In addition, this means selecting a emulsion on the basis of quality criteria processing method, the material of the material, cutting depth, cutting speed, Djourou fence Liang, and surface roughness, cutting oil, the shape of the device based on the emulsion, I will be the structure of the tank, filtration equipment also changes. In particular, acting bacteria is now breeding in response to the passage of time due to metal ion degradation due to heat generated hydraulic fluid leakage, humidity tung, during processing, seep from processing material at the time of processing the water-soluble cutting oil for generating the malodor by dropping significantly the performance of the cutting oil to corruption from, sometimes by introducing various additives to suppress spoilage in advance. In this study, we expect the effect of the cost reduction in the extension of fluid replacement cycle through the application of the management apparatus and deep understanding in the management of cutting fluid, the working environment through the understanding and interest of workers in the production site more than anything I try to become useful for the improvement.

• Journal of The Korean Society of Agricultural Engineers
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• v.53 no.4
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• pp.11-19
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• 2011

PADDIMOD2 was deloped to estimate nonpoint source pollution from paddy rice fields. The PADDIMOD2 was enhanced to estimate runoff and pollutant load during non-growing as well as growing season and to be easily used for public by development of Excel based system. Nutrient concentration and hydrology were based on Dirac delta function and continuous source function, and tank model for growing season and Event Mean Concentrations (EMCs) and SCS-Curve Number method for non-growing season. The PADDIMOD2 consists of three main component (input data, parameters data, and output data) by including eight Excel spread sheets. As a result of model application, total precipitation and irrigation were 1,051.7 mm and 439.2 mm, respectivley and surface runoff and water loss including infiltration and evapotranspiration were 463.0 mm and 947.9 mm, respectively. Annual nutrient loadings of T-N and T-P from study area were 6.7 kg/$km^2$/day and 0.5 kg/$km^2$/day, respectively. Development of PADDIMOD2 was focused on minimizing input data and maximizing user friendly system and is expected to be useful tool to evaluate various non-structure BMPs and estimate unit load from paddy rice fields for application at Korean TMDL.