• 한국지역사회생활과학회지
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• 제15권1호
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• pp.49-55
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• 2004

The purpose of this study is to research the antimutagenic and antioxidative effects of water dropwort and small water dropwort by Ames test and by measuring malondialdehyde(MDA) production. Water dropwort and small water dropwort were extracted with methanol and then further fractionated to hexane, chloroform, ethyl acetate, butanol and water, stepwise. The methanol extracts from both samples reduced the mutagenicities by aflatoxin $B_1(AFB_1)$ in Salmonella typhimurium TA 98. The production of MDA also decreased when the methanol extracts were added to the system. The hexane, chloroform and ethyl acetate fractions revealed higher antimutagenic activities against $AFB_1$ than the butanol and water fractions. Among the five fractions, the ethyl acetate fraction showed the highest level of antioxidant activity. From the results of the experiment, water dropwort and small water dropwort seem to be good antimutagenic and antioxidative sources of food.

• Preventive Nutrition and Food Science
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• 제13권2호
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• pp.117-121
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• 2008

The effects of water dropwort on the physical characteristics of tofu were investigated and consumer acceptance test was conducted. pH decreased significantly with increasing water dropwort concentration from 0 to 3%, whereas titratable acidity showed the reverse trend (p<0.05). The amount of water dropwort did not significantly influence the moisture content up to 2% substitution (p>0.05). Turbidity increased significantly with increasing water dropwort concentration (p<0.05). A significantly lower moisture content in 3% water dropwort tofu may be attributed to the denser structure and hardness of that was significantly higher than those of the other samples (p<0.05). Even though $a^*$-values of 3% water dropwort tofu was lower than that of control, $a^*$-values increased significantly with increasing water dropwort content (p<0.05). On the contrary, $b^*$-values decreased significantly with increasing water dropwort content (p<0.05). 2% water dropwort tofu was found to be the best with respect to its overall acceptability score.

• 한국유기농업학회지
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• 제8권1호
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• pp.79-88
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• 1999

In order top investigate the growth of water dropwort grown by sandculture irrigated with recirculated tilapia aquaculture water, these experiments were carried out. Fish(tilapia) production and biofiltration provided by sand cultured water dropwort(Oenanthe stolonifera DC.) were linked in a closed system of recirculation water. Water dropwort was irrigated with water drawn from the tilapia tank and drainage from sand beds was returned to the fish tank. The temperature, pH and EC of tilapia culture water were stable. The growth of water dropwort grown by sandculture with aquaculture water was normal. Microbial activity of the biofilterbed irrigated by tilapia rearing water was higher than that of biofilterbed irrigated by tapwater. The feasibility of an integrated, recirculatory system for concurrent production of water dropwort and fish with no additional fertilization application was demonstrated.

• 동아시아식생활학회지
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• 제20권4호
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• pp.589-595
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• 2010

The purpose of this study was to investigate the practical application and optimum conditions for adding functional dropwort, which is rich in physiological activity to Sulgidduk, toward developing dropwort-added dduk as a healthy food. To this end, samples of Sulgidduk with 0, 1, 3, 5 and 7% added dropwort powder were prepared, stored for 2 days at $20^{\circ}C$, after which the water content, chromaticity, and mechanical and sensory quality characteristics were measured. The results were as follows. The water content of nonglutinous rice powder and dropwort powder used for dropwort-added Sulgidduk was 41.73% and 3.5%, respectively. The water content decreased with increasing amounts of dropwort powder the group with 7% added dropwort powder was 33.88%, the lowest. For chromaticity, the L value decreased with increasing amount of dropwort powder with significant differences between groups with different added amounts (p<0.001). The a and b values increased with increasing amount of dropwort powder while they were 7.21 and 29.22, respectively, in the 7% dropwort powder-added group with significant differences between samples (p<0.001). For mechanical quality characteristics hardness decreased with increasing of amount of dropwort powder with significant differences between samples (p<0.001). There was no difference between samples in cohesiveness and springiness. Gumminess increased with increasing amount of dropwort powder with significant differences between samples (p<0.05). Chewiness and adhesiveness had a tendency to decrease with increasing amounts of dropwort powder. Overall acceptability was in the order of 3%, 5%, 1%, 7%, and 0% dropwort powder-added groups. Overall, for dropwort powder added Sulgidduk, the quality of flavor, color and taste was acceptable, compared to other powder-added groups. The 3% dropwort powder-added group was highly rated in general preference making it most desirable for making dropwort powder-added Sulgidduk.

• 한국식품영양과학회지
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• 제24권6호
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• pp.1016-1019
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• 1995

This study was conducted to investigate the nutrient composition of hydroponic water dropwort and the effect of blanching condition on ascorbic acid content. Nutrient composition of hydroponic water dropwort of mid harvest on the 30th day was measured in three portions of leaves, petioles and stems, and was compared with that of late harvest on the 40th day. Hydroponic water dropwort was obtained from the Department of Horticultural Science, Seoul National University. The results were as follows. The nutrients content of leaves were significantly higher in ash and ascorbic acid and lower in moisture, crude fat and crude fiber than those of stems and petioles. There was no significant difference in total vitamin A and thiamin contents between three portions. Ascorbic acid content of leaves harvested on the 30th day was the highest, 57mg/100g, and decreased to 18mg/100g in the leaves harvested on the 40th day. Marked increase in crude fiber and vitamin A content of leaves was observed in late harvest may be due to the maturation. The results of nutrient composition analysis suggest that the leaves of hydroponic water dropwort is important in ascorbic acid and ash. It is recommended that shorter blanching time and addition of 0.5% NaCl to the blanching water are better for higher ascorbic acid retention of hydroponic water dropwort. In conclusion, as hydroponic water dropwort has high content in ash, calcium, vitamin and free sugar with alkalinity, leaves as well as stems and petioles can be recommonded as a vegetable of high nutritional quality.

• Parasites, Hosts and Diseases
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• 제53권5호
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• pp.641-645
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• 2015

Fascioliasis, a food-borne trematode zoonosis, is a disease primarily in cattle and sheep and occasionally in humans. Water dropwort (Oenanthe javanica), an aquatic perennial herb, is a common second intermediate host of Fasciola, and the fresh stems and leaves are widely used as a seasoning in the Korean diet. However, no information regarding Fasciola species contamination in water dropwort is available. Here, we collected 500 samples of water dropwort in 3 areas in Korea during February and March 2015, and the water dropwort contamination of Fasciola species was monitored by DNA sequencing analysis of the Fasciola hepatica and Fasciola gigantica specific mitochondrial cytochrome c oxidase subunit 1 (cox1) and nuclear ribosomal internal transcribed spacer 2 (ITS-2). Among the 500 samples assessed, the presence of F. hepatica cox1 and 1TS-2 markers were detected in 2 samples, and F. hepatica contamination was confirmed by sequencing analysis. The nucleotide sequences of cox1 PCR products from the 2 F. hepatica-contaminated samples were 96.5% identical to the F. hepatica cox1 sequences in GenBank, whereas F. gigantica cox1 sequences were 46.8% similar with the sequence detected from the cox1 positive samples. However, F. gigantica cox1 and ITS-2 markers were not detected by PCR in the 500 samples of water dropwort. Collectively, in this survey of the water dropwort contamination with Fasciola species, very low prevalence of F. hepatica contamination was detected in the samples.

• 한국토양비료학회지
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• 제40권2호
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• pp.164-171
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• 2007

공단인근의 여러 중금속이 복합적으로 오염된 토양에서 중금속의 존재형태와 미나리 중 함량과의 관계를 평가하기 위하여 미나리 재배토양, 농수로 퇴적 저니토 및 대조토양과 동일지점에서의 미나리를 채취하여 분석 검토하였다. 토양의 중금속 총함량 중 Cd, Cu 및 Ni의 평균농도가 Kloke (1979)가 제안한 중금속의 허용한계치를 초과하였다. 중금속별 토양 내 주된 존재형태는 Cd이 치환태였고, 다른 중금속 (Cu, Zn, Ni 및 Pb)은 Fe-Mn 산화물 및 잔류 형태로 나타났다. 재배토양과 농수로 저니토의 형태별 함량에 의하여 환산된 잠재적인 중금속 이동계수는 Cd>Zn>Ni>Cu>Pb 순이였고, 특히 Cd의 경우 대조토양에 비하여 매우 높은 것으로 알수 있었다. 토양의 Cd 전함량과 토양 내 이동성이 높은 치환성 및 Fe-Mn 산화물 분포비율과는 정의 상관, 이동성이 낮은 잔류태와는 부의 상관을 보였다. 미나리 부위별 중금속은 대부분이 뿌리에 축적되었으며, 지상부로의 이행성은 Cd과 Zn가 줄기에서 높았고, Cu와 Ni 함량은 오히려 잎에서 높았다. 토양 중금속 중 Cd, Cu 및 Ni의형태별 함량과 미나리 중 함량과 관련성이 높았고, 특히 치환성 Cd과 Ni 함량은 미나리 중 함량과 고도의 정의상관을 보였다.

• 한국식품영양과학회지
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• 제22권4호
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• pp.405-411
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• 1993

본 연구는 녹황색채소류에서 추출되어 항암효과를 나타내는 활성 물질로 보고되어 있는 phytol과 들미나리 추출물을 sarcoma 180마우스에 주사한 후 적출한 비장세포내 T임프구와 T subset, 그리고 asialo GM1$^{＋}$세포를 정량하여 다음과 같은 결과를 얻었다. 1) 종양 마우스에 phytol 을 투여하였을 때 비장세포내의 T cell과 T-subset은 종양세포이식에 의해서 상승된 치를 더욱 증가시켰다. 그러나 들미나리추출물의 경우는 대동소이하였다. 2) Asialo GM1$^{＋}$세포는 종양마우스에 phytol이나 들미나리추출물을 주사하였을 때 모두 상승하였으며 정상마우스에 phytol 을 투여하였을 때도 대조군에 비하여 상승했지만 들미나리추출물을 작용시켰을 때는 저하되었다. 3) L3T4$^{＋}$/Lyt-2$^{＋}$세포비는 종양마우스에 phytol을 주사하였을 때 감소를 보였지만 정상 마우스에 투여하였을 때는 더욱 크게 낮아졌다. 그러나 들미나리추출물을 정상마우스에 투여 하였을때는 크게 감소하던 것이 종양마우스에 적요 시켰을 때는 증가현상을 보였다. 이상의 결과로 미루어 볼 때 phytol이나 들미나리추출물은 종양마우스에서 작용자 세포인 자연살해세포(natural killer cell)의 활성 인자로서 작용할 가능성이 높을 것으로 사료된다.

• The Plant Pathology Journal
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• 제18권3호
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• pp.109-114
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• 2002

To evaluate the phylogenetic and taxonomic status of the phytoplasmas associated with water dropwort (Oenanthe javanica DC) disease in Korea and Japan, their 16S rDNA was analyzed. DNAs extracted from water dropworts collected in Korea (Kyongnam province) and Japan (Chiba prefecture) affected by witches' broom and yellows were subjected to PCR using phytoplasma-specific primers, which amplified a 1.4-kbp fragment that included the 16S rDNA. Phytoplasmas were characterized by RFLP analysis using AluI, HaeIII, HhaI, KpnI, MseI, and RsaI restriction enzymes and by sequence analysis of the PCR products. The mater dropwort witches'broom (WDWB) and water dropwort yellows (WDY) 16S rDNA sequences were identical and closely related to onion yellows (OY, 99.9% identity), which belong to the aster yellows (AY) 16S-subgroup. However, the KpnI RFLP analyses clearly distinguished the WDY and WDWB phytoplasmas from the OY phytoplasma. The phylogenetic analysis based on 16S rDNA showed that WDWE and WDY phytoplasmas are members of a relatively homogeneous group that evolved from a common ancestor.

• 한국지역사회생활과학회지
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• 제16권2호
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• pp.3-9
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• 2005

The study was carried out to evaluate the antimutagenic and anticancer effects of small water dropwort. The methanol extracts from small water dropwort significantly reduced the mutagenicity induced by aflatoxin $B_1\;(AFB_1)$ and N-methyl-N'-nitro-N-nitrosoguanidine(MNNG) in Salmonella typhimutium TA 100. Also, the methanol extracts inhibited the growth of AZ-521 human gastric cancer cells and HT-29 colon cancer cells. The chloroform fraction from methanol extracts of small water dropwort inhibited $40\;to\;80\%$ of the mutagenicity by $AFB_1$ in Sal. typhimurium TA 100 by the addition of 2.5 to $10\%$. To separate active compounds, the chloroform fraction was subjected to column chromatography on a silica gel and separated into five fractions. Among the five fractions, fraction 4 showed the highest antimutagenic effect against $AFB_1$ and an anticancer effect in the HT-29 colon cancer cell. As the result of the analysis in GC-MS, 1-napthalene carbonitrile, 5,6,7,8-tetrahydrol and benzene, 1,1'-(1,4-pentadiene-1,5-diyl) bis-,(E,E) were identified potentially from fraction 4.