• Journal of the Korean Wood Science and Technology
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• v.24 no.1
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• pp.34-47
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• 1996

Veneers of two nominal thicknesses, 1.5 and 2.5mm, were rotary peeled from Japanese larch (Larix leptolepis). Dahurian larch (Larix gmelinei) and Radiata pine (Pinus radiata) bolts unheated and heated in the water vat temperature of $66^{\circ}C$ by rising a final temperature at 10cm core of $60^{\circ}C$ to remain core diameter of 11.4cm. 1.5 and 2.5mm thick veneer cut from the heated Japanese larch were significantly thinner than those of the unheated bolt and 2.5mm thick veneer cut from the heated Dahurian larch were significantly thicker than that of the unheated bolt. 1.5mm thick Dahurian larch veneer and 1.5 and 2.5mm thick Radiata pine veneers showed insignificant difference between the unheated and heated bolts, respectively. Check distance on the loose side of 1.5mm thick veneer cut from the heated Radiata pine was significantly wider than that of the unheated bolt. However check distances on the loose side of 1.5 and 2.5mm thick Japanese larch and Dahurian larch veneers and 2.5mm thick Radiata pine veneers showed insignificant difference between the unheated and heated bolts. Also the depth of check on the loose side of three species showed insignificantly difference between the unheated and heated bolts. Arithmatic mean deviation($R_a$) and maximum height($R_{max}$) of the profile on the loose side of dried veneer by preheating the bolt compared with unheated bolts were different among apecies and between veneer thickness. The preheating treatment slightly affected qualities of these thin veneers such as 1.5 and 2.5mm. The yield of 2.5mm thick veneer from the heated radiata pine was significantly higher than that of the unheated bolt. However the yield of 2.5mm thick veneer for other two species and 1.5mm thick veneer for three species showed insignificant difference between the unheated and heated bolts. The yield of 2.5mm thick veneer for three species were higher than those of 1.5mm thick veneer. The average yields of green veneer of Japanese larch. Dahurian larch and Radiata pine were 57.1, 55.1 and 54.0 percent, respectively. Variables such as initial MC. drying time and veneer thickness had strong effect for Japanese larch veneer, less effect for Radiata pine veneer and nuch less effect on final MC for Dahurian larch veneer in jet drying. Correlation between the current MC and the drying time of Dahurian larch with low variation of initial MC was higher than those of Japanese larch and Radiata pine veneer with high variation of initial MC in high temperature drying. Thickness shrinkages of 2.5mm thick veneer for Japanese larch and Radiata pine were higher than those of 1.5mm thick veneers, but shrinkages of Dahurian larch veneer were similar between two nominal veneer thicknesses.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.34 no.6
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• pp.643-651
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• 2001

Paddlewheel-driven circulation in a culture pond has been simulated based on the depth integrated 2 dimensional hydrodynamic model. Acceleration by paddlewheel is expressed as shaft force divided by water mass discharged by paddlewheel blades. The model has been calibrated and applied to culture ponds as following steps:- i) The model predicted velocities at every 10 m along longitudinal direction from the paddlewheel. The model was calibrated comparing the results with the measured values at mass correction factor $\alpha$ and dimensionless eddy viscosity constant $\gamma$, respectively, in a range $15\~20$ and 6. ii) Wind shear stress was simulated under conditions of direction $0^{\circ}C,\;90^{\circ}C\;and\;180^{\circ}C$ and speed 0.0, 2.5, 5.0 and 7.5 m/s. Change rate of current speed was <$1\%$ at wind in parallel or opposite direction to the paddlewheel-driven jet flow, while $4\%$ at orthogonal angle. iii) The model was then applied to 2 culture ponds located at the Western coast of Korea. The measured and predicted currents for the ponds were compared using the regression analysis. Analysis of flow direction and speed showed correlation coefficients 0.8928 and 0.6782 in pond A, 0.8539 and 0.7071 in pond B, respectively. Hence, the model is concluded to accurately predict circulation driven by paddlewheel such that it can be a useful tool to provide pond management strategy relating to paddlewheel operation and water quality.

• Applied Microscopy
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• v.24 no.2
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• pp.78-92
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• 1994

Lysozyme has been reported to be present in the secretory granules of the Paneth cell, and lysozyme immunoreactivity has been detected by immunogold method in Paneth cells of the intestine of human, mouse and rat. The present study was aimed at clarifying the intracellular distribution and changes of the lysozyme immunoreactivity in rabbit Paneth cell after common bile duct ligation of rabbit, using the electron microscope immunogold technique. Healthy adult rabbits weighing about 2kg body weight were divided into normal and bile duct ligated groups. Common bile duct ligation was performed aseptically under ether anesthesia. Experimental animals were sacrificed on the 1st, the 3rd, the 5th, the 7th and the 14th day after the operation. Mucosal specimens from the intestinal gland of ileum were fixed in 2.5% glutaraldehyde-1.5% paraformaldehyde, followed by 1% osmium tetroxide, embedded in araldite mixture, cut with LKB-V ultratome. Ultrathin sections were placed on parlodion coated nickel grids (200mesh). The section-bearing grids were floated upside down on the added substance in a moist chamber at room temperature except for the primary antibody step, which was at $4^{\circ}C$. Sections were etched with a saturated solution of sodium m-periodate for 60min. After etching, sections were pretreated with 0.02M tris buffered saline (TBS), pH 8.4, with 1% bovine serum albumin (BSA, Sigma) for 60min, then treated polyclonal rabbit anti-human lysozyme (Dakipatts) diluted 1 : 50 in TBS with 0.1% BSA for 20hr. Subsequently, grids were incubated 60min in biotinylated goat anti rabbit IgG (Amersham) diluted 1 : 100 in TBS with 0.1% BSA. After this, sections were incubated 60min on streptavidin gold G10 (Amersham) diluted 1 : 50 in TBS with 0.1% BSA. After each step, the grids were briefly rinsed with TBS with 0.1% BSA. After the strepavidin gold step, the sections were jet washed with distilled water. Counterstain of the sections performed by uranyl acetate and lead citrate, and observed with JEM 100 CX II electron microscope. Observed results were as follow; 1. Secretory granules of mouse Paneth cells have a lysozyme immunoreactivity and also eosinophil leucocyte of rabbit applied for the positive-control stain, are well labeld with gold particles. 2. Normal rabbit Paneth cells have a lysozyme immunoreactivity restricted on the secretory granules. 3. Amount lysosomes containing myelin figures in the Paneth cells were significantly increased from 5th day after the common bile duct ligation. 4. Immunoreactivity of Paneth cell secretory granules were more activated on the 3rd day after the common bile duct ligation as compared with those of the normal animal. But the lysozyme immunoreactivity were decreased from the 5th day after the common bile duct ligation. 5. Considering the above finding, lysozyme contained Paneth cell are affected following of common bile duct ligation, whereas lysosomes containing myelin-figure do not exhibit any immunoreactive relationship with those of secretory granules.

• Journal of Embryo Transfer
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• v.32 no.1
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• pp.1-8
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• 2017

Cryopreservation of germ cells from genetically proven animals could be a source of restoration tools from the risk of extinction or disappearance of wanted characteristics. Using frozen semen, the genetic gains of Korean native cattle have been increased greatly for 70 years. The preservation of genetic resources as a form of frozen semen straw has limited availability due to the numbers. To circumvent this weakness of frozen semen, we tested two re-freezing methods with different initial thawing temperatures using frozen Korean proven semen and rare breed semen from albino, black and chikso breeders. It has been known that human sperm could resist to cryo-damages by repeated freeze-thaw cycles, but not for Korean proven bulls number (KPN) or for rare breeds. Total 7 frozen semem from brindled(2), black(1), Korean Albino(2) and KPN(1) bulls were used for our research. After thawing straws under $5^{\circ}C/2min$ or $37^{\circ}C/40sec$ with low temperature water bath and thermo jug, spermatozoa were re-diluted with triladyl diluents after first thawing and re-frozen. Sperm motilities were compared between animals and treated groups after re-thawing. Mean values of motility and viability of refrozen/thawed sperm for expansion of the number of straws were significantly higher in $5^{\circ}C$ than in $37^{\circ}C$ (P < 0.05). However, the activity of viable sperm thawed at $5^{\circ}C$ was significantly decreased before refreezing. It is estimated that re-freezing of frozen semen from rare Korean native cattle is possible with resistant properties of survived spermatozoa.