• Title/Summary/Keyword: Washing procedure

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The Change of $SaO_2$, PFT and ABGA During the Bronchofiberscopy (기관지 내시경 검사에 따른 산소 포화도, 폐기능 및 동맥혈 가스의 변화)

  • Kim, Jong-Seon;Shin, Jeon-Eun;Kim, Tae-Hee;Chang, Jung-Hyun;Cheon, Seon-Hee
    • Tuberculosis and Respiratory Diseases
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    • v.45 no.3
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    • pp.574-582
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    • 1998
  • Background: Bronchofiberscopy is a procedure with a chance of airway irritation and it may cause pathophysiologic changes of respiratory system. So we tried to evaluate the influence of bronchofibercopy on $O_2$ saturation, ABGA and PIT by patient's basal status and procedure type. Method: $O_2$ saturation was measured every 1 minute from the left index finger tip with percutaneous oximetry. ABGA was done before and right after the bronchofiberscopy and PIT was done before and within 10 minutes after the bronchofiberscopy. Results: The mean time for bronchofiberscopy procedure was 14.5mim and $SaO_2$ maximally fall to 89.0 below 8% of the baseline after mean time of 8.4min, which was recovered at the end of the procedure. $SaO_2$ change amount was 8.4 % on Non-$O_2$ supply group, which was lower compared to 6.4 % of the $O_2$-supply group without statistically significance. Biopsy Group and BAL group showed more $SaO_2$ fall than washing only group. The level of $PaO_2$ and FEV1 of the patient didn't influence significantly on $SaO_2$ fall during the procedure. ABGA taken before and after the bronchofiberscopy showed mild fall of $PaO_2$ and mild rise of $PaCO_2$. Whereas PFT showed decrease of FEV1(P<0.05) and increase of RV without changes in airway resistance and pulmonary diffusion capacity. Comparing before and after the bronchofiberscopy, the washing group showed no significant changes on PIT, while the biopsy group and the BAL group showed increase of RV & decrease of $FEV_1$ after the bronchofiberscopy. BAL group showed more changing tendency rather than biopsy group although not statistically significant. Conclusion: Bronchofiberscopy is considered as a relatively safe procedure, but it would be better to be done with $O_2$ supply especially in the patient with low $PaO_2$ and in the case of biopsy and BAL.

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The Extraction Characteristics of Metal-contaminated Soil by Soil Washing (토양세척기법을 이용한 중금속 오염토양 처리에서 중금속 추출특성)

  • Hwang, Seon-Suk;Lee, Noh-Sup;NamKoong, Wan
    • Journal of Korean Society of Environmental Engineers
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    • v.27 no.10
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    • pp.1072-1080
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    • 2005
  • The extraction characteristics of heavy metals(HM) from a contaminated soil at existing lead smelters were investigated with ethylene diamine tetraacetic acid(EDTA), citrate and HCl as washing solutions. EDTA was more effective for Pb than for other heavy metals. As the mol ratio of EDTA/HM increased, the removal efficiency of heavy metals became higher. When the mol ratio of EDTA/HM approached to 6.5, it removed Pb most effectively. Citrate was effective especially in extracting Zn. The removal efficiency of HCl was comparatively high in almost all heavy metals, and at 0.3N concentration it was the highest. After soil washing process by the use of EDTA, the great part of exchangeable fractions and most of heavy metals of weakly adsorbed like carbonate fraction were extracted. For washing with citrate and HCl, four heavy metals showed the similar exchange of chemical partitioning and the exchangeable fractions of Pb which has weakly adsorbed to soil were more increased than before the process. As removal efficiency of citrate washing process depends upon the distribution of non-detrital fractions, so it can be contended that only the amount of non-detrital fractions could be removed from all the heavy metal content. EDTA and HCl could remove most of non-residual fractions in all heavy metals except Zn. As a result of EDTA washing, toxicity characteristic leaching procedure(TCLP) concentration of the processed soil met the USEPA Pb limit of 5.0 mg/L.

Effect of Washing on the Loss of Nutrients in Rice (세미(洗米)에 의한 미곡(米穀)의 영양손실(營養損失)에 관한 연구(硏究))

  • Cheigh, Hong-Sik;Ryu, Chung-Hee;Jo, Jae-Sun;Kwon, Tai-Wan
    • Korean Journal of Food Science and Technology
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    • v.9 no.2
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    • pp.170-174
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    • 1977
  • The comparative effect of washing on the loss of nutrients in rice was studied with regard to varieties of Japonica and Indica type and various polishing degrees. Washing procedure was made by usual method for the preparation of cooked rice. The average loss was 1-2% of total solid, 5-7% of protein, 18-26% of calcium, 19-47% of iron, 22-40% of vitamin $B_1$, 11-24% of vitamin $B_2$ and 36-45% of niacin during washing in the 50-90% polished rice of Japonica and Indica varieties. The Indica rice had relatively higher loss of total solid, protein, calcium and iron than that of Japonica rice. The loss of total solid, N-free extract and vitamin in 70% polished rice was lower than that of 90% polished rice. Loss of lysine in 90% polished rice amounted to more than 6% and was higher than the loss of other amino acids.

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Silver ions and nanoparticles in the making (은이온 은나노 만들기: 은나노 세탁기를 둘러싼 나노의 정의와 위험 및 규제 관련 논쟁의 분석, 2006-2012)

  • Yoo, Sang Woon
    • Journal of Science and Technology Studies
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    • v.13 no.2
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    • pp.173-206
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    • 2013
  • Launched by Samsung in 2003, nano-silver washing machines were a representative application of nanotechnology in commercial products until the US Environment Protection Agency (EPA) decided to regulate companies that produce nano-silver products in 2006. A year later, however, the EPA reclassified the washing machines not as an application of nanotechnology but as an ion-generating machine. As shown by the EPA's equivocation on this issue, establishing a category for nano-silver material should be considered in the procedure of risk assessment and regulation. This paper analyzes the controversy over Samsung's nano-silver washing machines more in detail to study how the demarcation between silver ion and nano-silver can vary according to the risk perception of nanotechnology. In Korea and the US, the boundary between silver ion and nano-silver was malleable and mobilzed depending on the contexts. Based on an analysis of the recent history of nano-silver washing machines, this paper explores the influence of risk perception over the ontological perspectives on a certain material.

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Dyeing Properties Resin Treatment Effects of the Lyocell Fabrics (리오셀 직물 수지가공 효과와 염색성)

  • Yoo, Hye-Ja;Lee, Hye-Ja
    • Journal of the Korean Society of Clothing and Textiles
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    • v.32 no.7
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    • pp.1095-1103
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    • 2008
  • The effects of resin finish and dyeabilities of four kinds of Iyocell fabrics that were manufactured by four kinds of pulps were investigated. The dyeabilities of Iyocell fabrics were similar, but differed from cotton fabric. In early stage of 30 minutes, cotton fabric was shown higher dye exhaustion ratio than Iyocell fabrics, however after then cotton fabric did not increase dye exhaustion, Iyocell fabrics increased continuously. At last, the dye exhaustion ratio of Iyocell fabrics were about 75% and that of cotton fabric was 65%. Two kinds of experimental procedures were applied for Iyocell fabrics. One was what the fabrics were treated with resins and washed with cellulase, and then dyed with reactive dyes. The other procedure was the fabrics were dyed with reactive dyes, and then applied the resin treatments and cellulase washing. After fibrillation and washing the undyed Iyocell fabric and the Iyocell fabric that was dyed with C.I.Reactive Red 120, their weight loss ratios were 3.5% and 2.8%, respectively. Dyeing with reactive dyes caused the crosslinking between cellulose and dyes and the crosslinking decreased fibrillation. The weight loss by enzyme washing of Iyocell fabrics decreased by the glyoxal and melamine resin treatments. The reduction of weight loss can be caused by fibrillation decrease. Dyeing and resin treating can be showed the synergic effect on the reduction of fibrillation. The effect of glyoxal resin on the reduction of fibrillation was a little better than that of melamine resin.

Conventional Cytology Is Not Beneficial for Predicting Peritoneal Recurrence after Curative Surgery for Gastric Cancer: Results of a Prospective Clinical Study

  • Kang, Ki-Kwan;Hur, Hoon;Byun, Cheul Su;Kim, Young Bae;Han, Sang-Uk;Cho, Yong Kwan
    • Journal of Gastric Cancer
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    • v.14 no.1
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    • pp.23-31
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    • 2014
  • Purpose: The role of peritoneal washing cytology in determining further treatment strategies after surgery for gastric cancer remains unclear. One reason for this is the fact that optimal procedures to increase the accuracy of predicting peritoneal metastasis have not been established. The aim of this study was to evaluate the efficacy of cytology using samples harvested from two different abdominal cavity sites during gastric cancer surgery. Materials and Methods: We prospectively recruited 108 patients who were clinically diagnosed with locally advanced gastric cancer (higher than cT1 stage disease). Peritoneal washing fluids were collected from the pouch of Douglas and the subphrenic area. Patients were prospectively followed up for 2 years to determine the recurrence and survival rates. Results: Thirty-three patients dropped out of the study for various reasons, so 75 patients were included in the final analysis. Seven patients (9.3%) showed positive cytology findings, of whom, three showed peritoneal recurrence. Tumor size was the only factor associated with positive cytology findings (P=0.037). The accuracy and specificity of cytology for predicting peritoneal recurrence were 90.1% and 94.2%, respectively, whereas the sensitivity was 50.0%. The survival rate did not differ between patients with positive cytology findings and those with negative cytology findings (P=0.081). Conclusions: Peritoneal washing cytology using samples harvested from two different sites in the abdominal cavity was not able to predict peritoneal recurrence or survival in gastric cancer patients. Further studies will be required to determine whether peritoneal washing cytology during gastric cancer surgery is a meaningful procedure.

Reduction of Polyspermy in Porcine in vitro Fertilization by Modified Swim-UP Method

  • Park, C.H.;B.S. Koo;Kim, M.G.;J.I. Yun;H.Y Son;Lee, S.G.;Lee, C.K.
    • Proceedings of the Korean Society of Developmental Biology Conference
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    • 2003.10a
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    • pp.110-110
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    • 2003
  • The high incidence of polyspermic fertilization is one of the major causes lowering the overall efficiency of porcine IVF. The common procedure for IVF involves the co-culture of both gametes in the medium drop, which increases sperm concentration and incidence of polyspermy. Therefore, the present study was carried out to increase the efficiency of porcine IVF by reducing polyspermy using a modified swim-up method. This method modifies conventional swim-up washing by placing oocytes directly at the time of washing. Sperm pellet was prepared in the tube and mature oocytes were placed on cell strainer with $70 \mu m$ pore size (Falcon 2350) at the top of the tube. After insemination, the oocytes were stained for examination. Also, the developmental potential of fertilized embryos was measured to evaluate for the feasibility of this method. While having similar penetration rates in both methods ($86.67 \pm 2.36% to 83.33 \pm 1.36%$), there was a significant reduction of polyspermy in modified swim-up method ($17.50 \pm 1.60%$) compare to the control ($44.1 \pm 3.70%$ (p<0.05). Subsequent culture showed higher rate of blastocyst formation in modified swim-up method (20.44$\pm$0.99%) than the control ($15.73 \pm 3.26%$) (P<0.05), even though there was no significant difference. These results suggest that, by controlling the number of spermatozoa reaching the oocytes, porcine oocytes might be protected from polyspermy in vitro. Also, the developmental potential of the fertilized embryos using this method could be improved by increasing the pool of spermatozoa with better quality. Further optimization of the procedure required to implicate this method in routine porcine IVF.

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The Preservation Treatment of Unlined Silk Jeogori Found at the Wooden Munsudongja Buddha Statue in the Sangwon Temple (상원사목조문수동자좌상 복장 명주홑저고리의 보존처리)

  • Kwon, Young-Suk;Baek, Young-Mee
    • Fashion & Textile Research Journal
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    • v.8 no.6
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    • pp.634-638
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    • 2006
  • The significance of this study is the demonstration of a new method of preservation procedure for national-treasure-class cultural-assets by examining the characteristics of the Jeogori relic, presumed to be that of King SeJo, and validating the preservation process. This Jergori as well as the wooden Munsu-donja Buddha statue from which they were found are designated as national treasure. The Jeogori relic is made of light silk and dimensions mark 77 cm in length, 67 cm in width, and 94 cm in sleeve length. It is a typical Jeogori worn in the Chosun Dynasty. Preservation procedure was in the following order: Removing dust and pollutants by vacuum, washing, removing creases, reinforcing the clothes, settling at paulownia backboard, and packing. Storage and exhibition of remains was executed with as minimal folding as possible, and microbiological damage prevention was performed by dyeing antibacterial treatment with eugenol onto supporting cloth. The relic, conservatively treated in accordance with the above procedure, is currently kept at the storage facility of the citadel Museum in the Woljeong Temple. It is displayed annually to the general public for approximately two weeks.

Characteristics and isolation of Listeria spp from poultry meat, products and environmental specimens in chicken slaughterhouse I. Isolation of Listeria spp (도계장 유래 닭고기와 부산물 및 환경재료에서 Listeria spp의 분리 및 분리균의 특성 I. Listeria spp의 분리)

  • Son, Won-geun;Kang, Ho-jo
    • Korean Journal of Veterinary Research
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    • v.31 no.3
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    • pp.271-277
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    • 1991
  • To investigate the epidemiological trait of listeriosis, Listeria spp were isolated from poultry meat, products and environmental specimens in chicken slaughterhouse. Also determined were isolation rates by the different enrichment procedures, the biochemical properties of isolates. In a total of 307 samples including poultry meat, liver, feathers, feces, chiller water, scalding water overflow and slaughterhouse floor, Listeria spp were isolated predominantly from scalding water overflow (90.0%), body skin before washing (66.7%), liver (20.0%) and feathers(15.0%) However, few Listeria spp were isolated from body skin after washing and feces. The higher isolation rates were obtained in the secondary enrichment procedure (7.2%) than in the primary enrichment (3.9%); after stored the secondary enrichment cultures for 2 weeks at $4^{\circ}C$, Listeria spp were present in 9.8%. The majority of the isolated Listeria spp were identical to those of the standards strains in biochemical and cultural properties. Overall, Listeria spp were present in 13.4% of the specimens tested, and were in order of prevalence of L innocua(11.1%), L monocytogenes(3.3%), L grayi(0.7%) and L murrayi(0.3%).

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Evaluation of Eye Irritation Potential of Solid Substance with New 3D Reconstructed Human Cornea Model, MCTT HCETM

  • Jang, Won-hee;Jung, Kyoung-mi;Yang, Hye-ri;Lee, Miri;Jung, Haeng-Sun;Lee, Su-Hyon;Park, Miyoung;Lim, Kyung-Min
    • Biomolecules & Therapeutics
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    • v.23 no.4
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    • pp.379-385
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    • 2015
  • The eye irritation potential of drug candidates or pharmaceutical ingredients should be evaluated if there is a possibility of ocular exposure. Traditionally, the ocular irritation has been evaluated by the rabbit Draize test. However, rabbit eyes are more sensitive to irritants than human eyes, therefore substantial level of false positives are unavoidable. To resolve this species difference, several three-dimensional human corneal epithelial (HCE) models have been developed as alternative eye irritation test methods. Recently, we introduced a new HCE model, MCTT HCE$^{TM}$ which is reconstructed with non-transformed human corneal cells from limbal tissues. Here, we examined if MCTT HCE$^{TM}$ can be employed to evaluate eye irritation potential of solid substances. Through optimization of washing method and exposure time, treatment time was established as 10 min and washing procedure was set up as 4 times of washing with 10 mL of PBS and shaking in 30 mL of PBS in a beaker. With the established eye irritation test protocol, 11 solid substances (5 non-irritants, 6 irritants) were evaluated which demonstrated an excellent predictive capacity (100% accuracy, 100% specificity and 100% sensitivity). We also compared the performance of our test method with rabbit Draize test results and in vitro cytotoxicity test with 2D human corneal epithelial cell lines.